Objective To analyze the impact of soldiers’ single-leg landing with load carriage on knee joint, and investigate its relationship with muscle strength, so as to provide references for daily load carriage training. Methods A total of 12 soldiers were required to perform single-leg landing from the 30 cm height without load carriage and with 15 kg load carriage,respectively.The kinematic and kinetic parameters were collected using Vicon motion capture system and AMTI force platform. The surface electromyography (sEMG) of anterior and posterior thigh muscles were also collected simultaneously, and the isokinetic strength during knee flexion and extension was tested. Results Compared with landing without load carriage, knee flexion angle and joint reaction force significantly increased during landing with 15 kg load carriage (P<0.05).The maximum sEMG of rectus femoris, lateral and medial femoral, biceps femoris increased significantly(P<0.05), but there were no significant differences in other indexes without and with load carriage(P>0.05). There was a significant negative correlation between peak moment of knee extension and vertical ground reaction force during single-leg landing without load carriage (P<0.05), while other indexes had no significant correlations (P>0.05). Conclusions Knee flexion angle and joint reaction force significantly increase during single-leg landing with 15 kg load carriage, the activation of anterior and posterior thigh muscles can relieved the ground reaction force during landing, and muscle strength plays some role in preventing landing injury.