Xintai Tablets,mainly composed of is the National Grade C of Chinese herb medicine newly developed by the Acadamy of TCM of Hunan Province,Institute of Chinese Herb Medicine.The new certification number of the Ministry of health is WEIYAOZHENGZI 1997-Z-055.Its clinical investigation was undertaken during four hospitals(the First Hospital Affiliated to Hunan College of TCM,etc.)in 1995.It suits well to those patients who suf- fered from the chest pain with the heart-blood stasis or heart-blood stasis and defifiency of heart-yin concomitantly.The clinical investigation on 307 cases indicated that the obvious ef- fective rate for the chest pain with angina pectoris is 56.02% with 93.48% of overall effec- tive rate,for the electrocardiogram is 34.38% with 62.28% of overall effective rate,for over 80% of stopping or reducing nitroglycerin is up to 40.69% ,over 50% of stopping or reducing nitroglycerin is up to 87.75%.The obvious effective rate of synthesis curative effect is 50.49% with 91.86% of overall effective rate.

BACKGROUND:Recent studies have found that stem cellpluripotency and differentiation is regulated by many long non-coding RNAs (LncRNAs). The expression and effect of LncRNA AK089560 during differentiation of stem cells is unclear. OBJECTIVE:To investigate the expression of LncRNA AK089560 in mesenchymal stem cells C3H10T1/2 undergoing osteogenic and adipogenic differentiation. METHODS:Osteogenic differentiation of mesenchymal stem cells C3H10T1/2 was induced by recombinant human bone morphogenetic protein-2 and evaluated using alkaline phosphatase staining. The adipogenic differentiation of mesenchymal stem cells C3H10T1/2 was induced by three factors (dexamethasone, indomethacin and insulin) and evaluated by oil red O staining. The dynamical expression of LncRNA AK089560 was detected by qRT-PCR assay. The AK089560 secondary structure was predicted using RNAfold software. The relationship between AK089560 and neighboring protein-coding genes was analyzed using UCSC genome browser and visualized by fancyGENE online software. RESULTS AND CONCLUSION:Over 70%of C3H10T1/2 cells were positive for alkaline phosphatase after osteogenic induction and more than 80%of the cells positive for oil red O staining after adipogenic induction. qRT-PCR results showed that the expression of LncRNA AK089560 at days 2, 4, 6 of both osteogenic and adipogenic differentiation was significantly decreased compared with the control group (P<0.05). Bioinformatics analysis showed that there was a stem-loop structure for AK089560 and sense overlap relationship between AK089560 and protein-encoding gene Sema3a. These findings indicate that LncRNA AK089560 expression is reduced during osteogenic differentiation and adipogenic differentiation, showing that AK089560 may be involved in regulating the multi-directional differentiation of stem cells.