Microwave-assisted extraction was optimized with response surface methodology for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii.The optimized extraction procedure was achieved by soaking the sample with 70% methanol（1∶15,v/v）for 30 min,and then microwave irradiation for 11 min at a power of 600 W.Coupling the extraction process with HPLC-fluorescence presented good recovery,satisfactory precision,and good linear relation.Compared with a method from the Chinese Pharmacopoeia,the proposed method enables higher extraction efficiency and more accurate analytical results.It can be of potential value in quality assessment of Radix Puerariae thomsonii medicinal materials.

Objective To investigate the related risk factors for dyed cornea of orthokeratology, and to analyze the related risk factors, and to determine the independent risk factors, Provide evidence for intervention measures. Methods The clinical date were investigated for 990 patients with fitting orthokeratology lens between may.2014 and may.2016 in our hospital,through access to medical records, follow-up visit,questionnaire investigation to find out the cause of orthokeratology adverse reactions, the related risk factors were analyzed by univariate and multivariate Logistic regression analysis, using SPSS17.0 statistical processing. Results The incidence rate of dyed cornea of orthokeratology lens fitting in was 14.55%(144 in 990 patients); the single factor analysis found gender, age, region, refraction, lens position, family members, no significant difference . Eye disease combined (χ2= 28.73, P＜0.01), Schirmer I test (χ2=17.68, P＜0.01), lens activity (χ2=67.1, P＜0.01), Lens deposit(χ2=64.29, P＜0.01), lens wearing time (χ2=43.25, P＜0.01), health habits (χ2=38.01, P＜0.01) and water resources (χ2=3.81, P＜0.05), the difference was statistically significant; Logistic Logistic regression analysis found that Schirmer I test ( OR=4.126, P=0.003), lens activity ( OR=1.733, P=0.104), Lens deposit( OR=3.723, P=0.038), lens wearing time ( OR=5.034, P=0.002), health habits ( OR=6.544, P=0.002) and water resources ( OR=7.501, P=0.002) were independent risk factors for adverse reactions of orthokeratology. Conclusions Intervention measures that improve the fitting technology, complete removal of lens depositthe, Control wearing time, improve the health behaviors of the patients with the habit, use saline and professional cleaning are of great significance to reduce the incidence of adverse reactions of orthokeratology.

Objective To explore the risk factors for adverse reaction of Ortho-K lens so as to provide a reference for formulating targeted intervention measures.Methods From May 2014 to May 2016, retrospective analysis was used to clinical data from 990 patients with Ortho-K lens of Hankou Hospital, Wuhan Aier Eye Hospital. The reasons for adverse reaction of Ortho-K lens were found out through consulting medical record, telephone follow-up or asking and the related risk factors were analyzed.Results Among 990 patients with Ortho-K lens, there were 144 of them with adverse reaction with 14.55％ for the incidence. Single factor analysis showed that there were statistical differences in history of ocular disease, schirmer tests, mobility of eyeglass, sediment of eyeglass, time of wearing glasses, health habit, cleaning solution for eyeglass among patients with Ortho-K lens (χ2=28.73,17.68,67.1,64.29,43.25,38.01,3.81;P<0.05). Multivariate Logistic regression analysis revealed that the independent risk factors for adverse reaction of Ortho-K lens included secretion of tears, mobility of eyeglass, sediment of eyeglass, time of wearing glasses, health habit and cleaning solution.Conclusions The major factors for adverse reaction of Ortho-K lens are schirmer tests, mobility of eyeglass, sediment of eyeglass, time of wearing glasses, health habit and cleaning solution. Hospitals should constantly optimize the process, improve optometry technology, promote healthy behaviors of patients to make them form a good living and health habit as well as eye care habit, strengthen education and guidance to ensure patients master correct cleaning and disinfection methods, effectively control time of wearingglasses. We recommend the 0.9％ sodium chloride solution and professional cleaning solution to deal with eyeglass and nursing devices.