1.Effects of rolipram on learning and memory and the activity of PDE4 in hippocampus following the focal brain injury induced by ischemia-reperfusion in rats
Xu LIU ; Yufang CHENG ; Hanting ZHANG ; Jiangping XU
Chinese Journal of Pathophysiology 1989;0(06):-
AIM:To investigate the effects of rolipram on the ability of learning and memory and the activity of PDE4 in hippocampus following the focal brain injury induced by ischemia-reperfusion in rats.METHODS:The cerebral ischemia-reperfusion injury model was made by middle cerebral artery occlusion(MCAO) in rats.The rats were randomly divided into sham-operated group,model group,and rolipram group.Rolipram was administered once a day(1 mg/kg,ip) from 6 h after the onset of the operation for 2 weeks.Then the learning and memory abilities were tested after Morris water maze and step-though training.The activity of PDE4 in hippocampus was evaluated by HPLC.RESULTS:In the Morris water maze test,compared to sham-operated group,the platform-finding time and swimming distance in model group were significantly longer(P
2.Biological assessment of a new synthetic macromolecular resin class of comfort denture adhesive.
Ke ZHAO ; Man WANG ; Xiangrong CHENG ; Qun XIAO ; Zhian LI ; Hanting CHENG
Journal of Biomedical Engineering 2003;20(3):415-417
This article devotes itself to evaluating the biocompatibility of synthetic macromolecular resin class of Comfort denture adhesive, which was developed by the present authors. Acute toxicity, haemolysis, cytotoxicity, sensitization and oral mucous stimulation were tested with standard method (ISO7406-1997 and YY0268-1995). The results showed that no toxic effect was observed with in vivo tests and no cytotoxic effect was observed with in vitro MTT assay. Haemolysis rate of the material (2.95%) indicated good hemaocompatibility. No local mucous membrane irritation reaction was noted after the relevant tests. The developed Comfort denture adhesive exhibited good biocompatiblility.
Adhesives
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pharmacology
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Animals
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Cells, Cultured
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Denture Retention
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Denture, Complete
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Humans
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Materials Testing
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Mouth Mucosa
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drug effects
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physiology
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Rabbits
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Rats
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Resins, Synthetic
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pharmacology
4.A new diphenyl ether from the endophytic fungus Verticillium sp. isolated from Rehmannia glutinosa.
Wei PENG ; Fei YOU ; Xiao-Li LI ; Min JIA ; Cheng-Jian ZHENG ; Ting HAN ; Lu-Ping QIN
Chinese Journal of Natural Medicines (English Ed.) 2013;11(6):673-675
AIM:
To investigate the chemical constituents of the endophytic fungus Verticillium sp. isolated from Rehmannia glutinosa.
METHODS:
The compounds were isolated and purified by repeated column chromatography, and their structures were determined on the basis of physicochemical properties and spectral analysis. Their cytotoxic and antifungal activities were evaluated.
RESULTS:
Ten compounds were obtained and their structures were identified as 2, 4-dihydroxy-2', 6-diacetoxy-3'-methoxy-5'-methyl-diphenyl ether (1), paecilospirone (2), α-acetylorcinol (3), 2-methoxy-1,8-dimethyl-xanthen-9-one (4), 4-hydroxy-α-lapachone (5), enalin A (6), 2,3,4-trimethyl-5,7-dihydroxy-2,3-dihydrobenzofuran (7), 4-hydroxyethyl-phenol (8), 2,4-dihydroxy-3,5,6-trimethyl- methylbenzoate (9), and 3-isopropenyl-(Z)-monomethyl maleate (10).
CONCLUSIONS
Compound 1 is a new diphenyl ether, and showed cytotoxic activity against HL-60 cells (IC50 2.24 μg · mL(-1)), and antifungal activities against Candida albicans (MIC 8 μg · mL(-1)) and Aspergillus fumigatus (MIC 16 μg · mL(-1)).
Antifungal Agents
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chemistry
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isolation & purification
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metabolism
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pharmacokinetics
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Antineoplastic Agents
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chemistry
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isolation & purification
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metabolism
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pharmacokinetics
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Aspergillus fumigatus
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drug effects
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Candida albicans
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drug effects
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Cell Line, Tumor
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Endophytes
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chemistry
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metabolism
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Humans
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Phenyl Ethers
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chemistry
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isolation & purification
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metabolism
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pharmacokinetics
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Rehmannia
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microbiology
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Verticillium
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chemistry
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metabolism