Objective To evaluate the clinical application value of influence of chylemia specimen before and after blood plasma exchange on the detection results of hematological analyzer.Methods 22 chylemia specimens were collected,then the hematological analyzer was used to test the chylemia specimens before and after blood plasma exchange,and the results were compared with those detected by the manual microscopic counting.Results The detection results of WBC,NEU,LY,MO,RBC,HGB and PLT in the non-chylemia specimens and the original blood specimens before blood plasma exchange had no statistically significant differences (P >0.05);in 22 cases of chylemia specimens,the WBC count and classification,and the mean value of HGB and PLT before and after blood plasma exchange in the chylemia specimens had statistically significant differences (P <0.05),the WBC count and clas-sification,and PLT mean value after blood plasma exchange in the chylemia specimens by the hematological analyzer had good corre-lation with those detectd by the microscopic counting method(r2 =0.936,r2 =0.918).In the triglyceride interference test,the WBC count and classification,and the mean value of HGB and PLT could be changed with the increase of the triglyceride concentration. Conclusion Adopting the blood plasma exchange method could obviously improve the influence of chylemia specimens on the detec-tion results by the hematological analyzer and provide the accurate results for clinic.

Objective To evaluate the usefulness and necessity of spiral computer tomography (SCT) and color-Doppler ultrasonography (CDU) in primary hepatocellular carcinoma (HCC) after transcatheter arterial chemoembolization (TACE). Methods Plain SCT scanning and contrast-enhanced SCT scanning or / and CDU were performed for 68 HCCs before and four weeks after TACE. Factors related to overall survival (OS) were observed as tumor size necrosis, relapse, lipiodol deposition, blood supply of the survived tumor. Images of SCT and CDU were compared. Clinical significance obtained through direction of diagnostic radiology was evaluated. Results 139 masses observed in the 68 cases were shranked or necrotized in a different degree after TACE. 8 new metastasized massed were observed. Complete lipiodol deposition was observed in 27 masses and no lipiodol deposition in 14 masses. As for estimating the types of lipiodol deposition, SCT was better than CDU with significant difference. SCT could indirectly estimate the blood supply of tumor after TACT through 3 dynamic enhanced phases, while CDU could directly visualize the hemodynamic changes of liver, internal and peripheral parts of the tumor. Conclusion TACE is an effective way to treating HCC. SCT scanning and / or CDU are necessary before and after TACE to estimate the imaging changes so as to provide indispensable information for the best therapeutic schedule in the future.

AIM: To evaluate the role of human angiotensin Ⅱ(AngⅡ) type Ⅰ receptor (AT1R) antisense cDNA (ahAT1) on migration of cultured artery smooth muscle cells (VSMCs). METHODS: Two recombinant adenoviral vectors, Ad/CMV. ahAT1 containing full length antisense cDNA targeting to human AT1R mRNA, and Ad/CMV.LacZ containing LacZ called report gene, were constructed by orientation clone technology and homologous recombination, and then were used to transfect VSMCs in vitro. AT1R expression detected by RT-PCR and immunohistochemistry, and migration of VSMCs measured by Boyden's Chamer methods, were compared between transfected and non- transfected VSMCs. RESULTS: Forty-eight hours after Ad/CMV. ahAT1 transfection, the level of AT1R mRNA decreased markedly (50% of control group), and AT1R protein expression was significantly less (P