OBJECTIVE To in vestigate the existing problems about store construction and market operation and management of DTP （direct-to-patient）pharmacies in Guizhou province ，and to provide countermeasures and suggestions for the improvement and development of professional operation of DTP pharmacies in Guizhou province. METHODS According to the literature review ， the questionnaire was designed. Then the field survey of 11 DTP pharmacies and questionnaire survey for the person in charge （or store managers ） in Guizhou province were conducted from April to May 2021. The questionnaire mainly included the basic information，online sales channel construction ，pharmaceutical logistics distribution ，pharmaceutical care ，vocational training ， construction of pharmacy management system and application of drug management information system etc. ，in order to analyze the inadequacies of market operation and management of DTP pharmacies in Guizhou province and put forward suggestions. RESULTS Totally 11 questionnaires were delivered and 9 valid questionnaires were retrieved ，the effective response rate of questionnaires was 81.82％ . The sample pharmacies were all from Guiyang ，most of them （77.78％）were established by local pharmaceutical enterprises in Guizhou province ，and only 22.22％ of the sample pharmacies had opened online drug purchase channels. In terms of pharmaceutical care ，55.56％ of the sample pharmacies provided basic testing and life style interventions respectively ，44.44％ of the sample pharmacies provided adverse drug reaction monitoring ，and 33.33％ of the sample pharmacies implemented health education presentations and chronic disease rehabilitation program respectively. In term of personnel training ，only 11.11％ of the sample pharmacies implemented weekly business training ，and 66.67％ of the sample pharmacies received innovative and academic pharmacy information training. The construction of DTP pharmacy management systems in Guizhou province was completed basically ， but in terms of application of drug management information system ， 22.22％ of the sample pharmacies conducted drug application analysis and monitoring. CONCLUSIONS The development of DTP pharmacies in Guizhou province is still in initial stage currently. The convenience and accessibility of medicines for patients and the construction of online sales channel need to be improved ；the internet channel construction is slow ；an effective selection and evaluation mechanisms for pharmaceutical logistics enterprises need to be created ；pharmaceutical care ability and pharmacy service personnel training system need to be strengthened ；informatization management and industrial recognition of DTP pharmacies in Guizhou province need to be promoted. Therefore ，the enterprises ， government， universities and associations should work together and strengthen the professionalization ， informatization， institutionalization and standardization of DTP pharmacies in Guizhou province ，in order to provide patients with a convenient drug sales channels and high-quality personalized pharmaceutical care platforms eventually.