1.SERUM CHYOMIUM RELATED TO DIABETES
Huifang WANG ; Hanmei GOU ; Qing DONG
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(03):-
The auther .measured the concentration ofchromium in serum of the normal for 30 and 30 pa-tients with diabetes. The results indicate that thechromium level in serum of diabetes is significantlylower than that of the normal (P
2.EXPERIMENTAL OBSERVATION OF TRANSPLANTATION OF CULTURED NEWBORN PIG PANCREATIC ISLET CELLS
Hanmei GOU ; Huifang WANG ; Wei JIANG ; Li XU ; Hui GAO ;
Journal of Xi'an Jiaotong University(Medical Sciences) 1982;0(01):-
In this article, we report the pancreatic islet cells function of newborn pig pancreatic tissues cultured by Ham F_(10), and the condition of graft survived into the bodies of Sprague-Dawley rats, and also study the influence of Glycyrrhiza uralensis to the grafts. The cultured islet cells of newborn pig were transplanted into the mesenteric cavities (the first & second group), kidney cystic(the third group) and brain tissucs (the fourth group) of the 4 groups adult Sprague-Dawley rats. The first group rats drank Glycyrrhiza uralensis water, after 8~11weeks of operation a examination pathological was made with the cut graft The results showed that the first and second group grafts survived over 11weeks, and survived better the group drunk the Glycyrrhiza uralensis water was much better than other. The purpose of this experiment is to find the heteropolar source of pancreatic tissues offering body.
3.A study on mechanism of lncRNA-mediated SNHG5/miR-26a-5p/MTDH signal axis promoting metastasis of colorectal cancer
Junling YE ; Xiaoying ZHENG ; Xinjian GUO ; Ruihui CHEN ; Liu YANG ; Xiaodan GOU ; Hanmei JIANG
China Oncology 2023;33(7):673-685
Background and purpose: Long non-coding RNA small nucleolar RNA host gene 5 (lncRNA SNHG5) plays a cancer-promoting role in many cancers, however its effect on colorectal cancer (CRC) and its regulatory mechanism are not clear. This study aimed to explore the mechanism of lncRNA SNHG5/miR-26a-5p/metadherin (MTDH) signal axis promoting metastasis of CRC. Methods: The data of The Cancer Genome Atlas (TCGA) database was analyzed, the abnormal expression of lncRNA in CRC was explored and analyzed the survival. Samples of CRC, paracancerous tissues and complete clinical data of patients who underwent surgical resection from October 2020 to October 2021 were collected. The expression levels of SNHG5 and miR-26a-5p in lncRNA were detected by real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR), and the expression level of MTDH was detected by immunohistochemistry. The relationship between the relative expression level of lncRNA SNHG5 in CRC and clinicopathological features and survival time was analyzed. The effects of lncRNA SNHG5 on the proliferation, migration and invasion of CRC cells were detected by cell counting kit-8 (CCK-8), clone formation, scratching assays, transwell test and in vivo xenotransplantation. The relationship between CRC cell metastasis, the expression level of epithelial-mesenchymal transition related molecules and lncRNA SNHG5 expression level by Western blot and immunohistochemical detection were explored. The physical interaction between SNHG5 and miR-26a-5p, MTDH and miR-26a-5p was studied by RNA pull-down test, double luciferase reporter gene detection and RNA co-immunoprecipitation. The functional relationship among the three was verified by CCK-8, EdU and transwell experiments. The effect of SNHG5, miR-26a-5p and MTDH expression on migration and invasion related molecules was analyzed by Western blot. Results: The results of TCGA database analysis showed that lncRNA SNHG5 was significantly upregulated in CRC. The results of RTFQ-PCR and immunohistochemistry showed that the levels of lncRNA SNHG5 and MTDH in CRC tissues were significantly upregulated (P<0.05), the level of miR-26a-5p was decreased (P<0.05), and the level of MTDH in samples with high expression of SNHG5 was also increased. The expression of lncRNA SNHG5 in CRC tissues with serosa and extraserosal invasion, distant metastasis, lymph node metastasis and TNM stage Ⅲ was significantly higher compared with subserosal invasion, no distant metastasis and lymph node metastasis and TNM stage Ⅰ-Ⅱ (P<0.05). The results of survival analysis showed that the high expression of lncRNA SNHG5 was significantly correlated with overall survival rate (P<0.05). Overexpression of lncRNA SNHG5 could enhance the proliferation, clone formation, migration and invasion of CRC cells, promote the growth and lung metastasis of transplanted tumor, increase the relative expression level of Ki-67 proliferation index and vimentin (P<0.05), and decrease the relative expression level of E-cadherin (P<0.05). However, the development of CRC cells was inhibited after inhibition of lncRNA SNHG5 expression. RNA pull-down test, double luciferase reporter gene detection and RNA co-immunoprecipitation confirmed the physical interaction between SNHG5 and miR-26a-5p, MTDH and miR-26a-5p. Upregulation of miR-26a-5p or downregulation of MTDH expression in lncRNA SNHG5 overexpressed cells partially reversed the effects of lncRNA SNHG5 on proliferation, migration, invasion and expression of related molecules in CRC cells. Conclusion: LncRNA SNHG5 is upregulated in CRC tissues and cells, and its high expression is related to tumor progression and poor survival. It can be used as a molecular sponge of miR-26a-5p to regulate the expression of MTDH to promote the proliferation and metastasis of SW620 cells.