a-Terpineol as an another antiasthmatic principle of essential oil of Artemisia argyi was studied. It had relaxant effect on isolated guinea pig tracheal smooth muscle preparations, the effective threshold concentrations being between 2. l-4.2x10-5 g/ml, and the EC50 being 0.71-1.15x10-4g/ml. It could protect guinea pigs from the acetylcholine and histamine-induced asthma when given orally or aer-osolly. The level of cAMP in the tracheal smooth muscle of guinea pigs was elevated in the presence of l0-4g/ml a- terpineol. The drug could block the development of desensitization to isoproterenol on isolated guinea pig tracheal smooth muscle preparations, and its relaxant effect did not be- influenced on the desensitized preparations also. In addition, a-terpineol had the Inhibitory effect of anaphylactic mediators release antagonistic effect of the mediators, antitussive and expectorant effect, and the inhibitory effect on isolated ed guinea pig atria. The toxiicological studies showed that it had good safety margin. ( + ) - a-Terpineol amd (-) a -terpineol had similarquality and potency of actions.

?-caryophyllene was one of the effective antiasthmatic principles of essential oil of Artemisia argyi, by way of structural modification to form ?-caryophyllene alcohol. P-caryophyllene alcohol could protect acetylcholine and histamine-induced asthma in guinea pig, and the effect was longer than 4h. It had relaxant effect on isolated guinea pig tra.cheal smooth muscle prepareations, and the pD2 values was 4.36 + 0.37. The drug not only significantly inhibited antigen induced release of SRS-A in the lung fragments of sensitized guinea pig( ID50 23mg/L ), but also inhibited SRS-A induced contraction of the guinea pig ileum(ID50 18mg/L). In addition, P-caryophyllene alcohol had antitussive and expectora'at actions. These results show that p-caryophyllene alcohol is a new antiathmatic drug.

OBJECTIVETo investigate the fluctuations in arterial leukocyte phospholipase D (PLD) activity during the perioperative period of open heart surgery under cardiopulmonary bypass (CPB), and the relationship between PLD activity and systemic inflammatory response induced by CPB.

METHODSArterial blood was obtained from 26 patients undergoing open heart surgery at 8 different time points during the perioperative period, from which leukocytes were isolated for determination of PLD activity, CD11b expression and myeloperoxidase (MPO) activity. Plasma IL-6, IL-8 and C-reactive protein were also determined. The 26 cases were retrospectively divided into 3 groups according to perfusion time in order to detect the possible influences of CPB on PLD activity and IL-6 and IL-8 levels.

RESULTSWhen the ascending aorta was declamped, average arterial leukocyte PLD activity was 0.305 +/- 0.132 nmol choline.min(-1).mg(-1), 5.0 times higher of the pre-CPB value, and remained (5.4 times higher of the pre-CPB level) at 72 hours after CPB. Leukocyte CD11b expression and plasma IL-6 and IL-8 levels increased significantly at the end of CPB, while MPO activity and C-reactive protein concentration reached their peaks at 1 and 24 hours, respectively, after CPB. At the end of CPB, the arterial leukocyte PLD activity of patients whose CPB duration was longer than 90 minutes were 1.82- and 1.74-fold that of the other two groups with CPB lasting between 90 and 60 minutes and less than 60 minutes.

CONCLUSIONSArterial leukocyte PLD activity rises significantly in CPB and its elevation is earlier and more persistent than other inflammation-related indicators tested; longer CPB duration leads to higher leukocyte PLD activity at the end of CPB. These results imply that PLD could be a new target for prevention of systemic inflammatory response induced by CPB.

Adolescent ; Adult ; Cardiopulmonary Bypass ; Female ; Humans ; Interleukin-6 ; blood ; Interleukin-8 ; blood ; Leukocytes ; enzymology ; Male ; Middle Aged ; Phospholipase D ; blood ; Systemic Inflammatory Response Syndrome ; enzymology

Purpose To explore the value of dual-layer spectral detector CT(DLSCT)in evaluating preoperative tumor staging in rectal adenocarcinoma(RA).Materials and Methods A total of 78 patients with pathologically confirmed RA in Guangdong Provincial Hospital of Chinese Medicine from May 2021 to March 2022 were involved in this retrospective study.All the patients underwent plain and dual-phase contrast-enhanced scans by DLSCT within one week before surgery.Taking pathological results as the golden standard,the accuracy rates of tumor staging were calculated and compared between the multiple-parameter maps derived from DLSCT and 120 kVp polyenergetic image.The effective atomic number(Z-eff)from plain scan,iodine concentration(IC)from arterial phase(AP)and venous phase(VP)were measured.The normalized iodine concentration(NIC)in AP and VP were calculated.The differences of Z-eff,NICAP and NICVP were compared among the pT1-2,pT3 and pT4 groups.The correlation between the pT stages and above values was assessed and the diagnostic efficiencies were analyzed.Results The overall accuracy rate(88.46%vs.67.95%;χ2=9.628,P=0.002),the pT1-2 staging accuracy rate(80.00%vs.40.00%;χ2=6.667,P=0.01),and the pT3 staging accuracy rate(88.10%vs.69.05%;χ2=4.525,P=0.033)of multiple-parameter maps derived from DLSCT were significantly higher than those of 120 kVp polyenergetic image,respectively.The Z-eff,NICAP and NICVP were significantly different among pT stage groups(F=6.456,11.029,12.698,all P<0.05)and exhibited a positive correlation with pT stages(r=0.371,0.367,0.363,all P<0.01).The areas under the curve of Z-eff,NICAP and NICVP to assess pT3-4 staging RA were 0.77,0.71 and 0.74,respectively.Conclusion The multiple-parameter maps derived from DLSCT can significantly improve the diagnostic accuracy of preoperative tumor staging of RA.Z-eff from plain scan and NIC from dual-phase helps differentiate pT1-2 from pT3-4 staging RA.