Objective To observe the effect of the rivaroxaban and low molecular weight heparin calcium to prevent deep vein thrombosis (DVT) after artificial joint replacement. Methods 167 patients included 114 cases for total hip arthroplasty and 53 cases for total knee replacement from Novermber, 2009 to October, 2010 were divided into two groups: observation group (n=84) was treated with rivaroxaban tablets, 6 h after surgery, 10 mg/d, 2 weeks for knee arthroplasty, 5 weeks for total hip arthroplasty; and control group (n=83) was treated with low molecular weight heparin calcium 2500 IU 2 h before and after surgery for 5 d. Ultrasonography was used to detect the deep vein lumen size change and DVT rate of bilateral lower extremities in all patients before and after surgery. Activated partial thromboplastin time (APTT) was tested before surgery, and 2 d, 5 d, 7 d, 14 d, 35 d after surgery. Results ① Incidence of DVT showed that there were 9 cases (10.7%) with DVT in the observation group; there were 15 cases with DVT (18.1%) in the control group. ② Lower limb deep vein lumen size change comparison: there were 23 cases (27.4%) with superficial femoral vein stenosis more than 2 mm in the observation group while 38 cases (45.8%) in the control group, there were 27 cases (32.1%) with popliteal veins stenosis more than 2 mm in the observation group and 47 cases (56.6%) in the control group (P<0.01). ③APTT: APTT prolonged >10 s was 0 in the observation group while 23 cases (27.7%) in the control group (P<0.01). Conclusion Trivaroxaban may prevent DVT after artificial joint replacement and be better than low molecular weight heparins calcium.

Objective To compare the difference of spatial reference memory and dopamine (DA) level in the brain between acute,subacute and chronic mouse model of Parkinson' s disease(PD) induced by 1-methyl-4-phenyl-1 ,2,3,6-tetrahydropyridine(MPTP) injection. Methods The acute,subacute and chronic mouse model of Parkinson' s disease were induced by injecting the same MPTP volume dose with different schedules. The spatial reference memory of mice was tested by morris water maze. Dopamine concentration in striatum, hippocampus and the prefrontal cortex were detected with HPLC. The number of tyrosine hydroxylase(TH)-positive cells in the substantia nigra of mice was detected by immunohistochemistry. Results The mean escape latency of the chronic but not the acute or subacute mouse model of Parkinson' s disease was significant longer ( P ＜ 0.05 ) than its control group. The striatum DA concentration of three test groups ( ( 1180. 1 ± 293.0 ) ng/ml, ( 1177.4 ± 450.5 ) ng/ml,( 1149.6 ± 353.0 ) ng/ml ) reduced significantly compared to their control groups ( ( 225.6 ± 79.7 ) ng/ml, ( 273.6± 64.9 ) ng/ml, ( 327. 1 ± 126.2 ) ng/ml, P ＜ 0.01 ). The prefrontal cortex DA concentration of the acute mouse model of Parkinson' s disease ( ( 65.3 ± 23.9 ) ng/ml ) was significant lower than its control group ( ( 41.2 ±18.8 )ng/ml, P ＜ 0.05 ). No significant changes of hippocampus DA concentration were seen between these test groups and their control groups. The number of TH positive cells in substantia nigra significantly decreased in three test groups compared to their control groups( P ＜ 0.05 ). Conclusion The difference of spatial reference memory between three regimens of the mouse model of Parkinson' s disease may not due to the difference of DA level in their brain.

Objective To investigate the anxious behavior in acute parkinson's mice that were induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) injection.Methods Twenty mice were randomly divided into the control group (n =10) and model group(n =10);The model group was induced by injecting MPTP dosage,and the control group was induced by the same dose of saline.The anxious behaviors in mice were tested by the elevated plus-maze test and the light/dark box.Results The model group mice spent a longer time than the control group in the dark box (P ＜ 0.05).The open arm entry (OE),open arm time (OT) and OE％ of model group was significantly less than that in control group in the elevated plus-maze test (P ＜ 0.01),the OT％ was significantly less than control group (P ＜0.05).Conclusions Anxiety symptoms appeared in the model group of early parkinson disease (PD)mice.

Objective: To investigate the effective of Sanhua decotion(三化汤) on early brain water content,penetration of blood brain-barrier and the expression of aquaporin4(AQP4) in rats with acute cerebral ischemia/reperfusion(I/R).Methods: The string inserting method was employed to reproduce the rat model of focal cerebral I/R SD rats were randomly divided into sham operation group,model group and Sanhua decotion group.After I/R,each group was then divided into 6 hours,12 hours,24 hours,48 hours and 72 hours five subgroups.The brain water content was detected by the ratio of wet/dry weight to evaluate cerebral edema and the blood-brain barrier(BBB) damage was observed by Evan′s blue(EB) staining.The AQP4 mRNA and protein expressions were measured by reverse transcription-polymerase chain reaction(RTPCR) and immunohistochemistry respectively.Results: Compared with sham operation group,the brain water content,EB content,AQP4 protein and AQP4 mRNA expressions were increased at every time point after I/R in the model group(P

Purpose To investigate the expression of DLC1 and its relationship with Ki-67 in cancerous and non-cancerous tissues of the breast.Methods In situ hybridization and immunohistochemiscal EnVision method were used to detect the expression of DLC1 mRNA and protein and Ki-67 in 52 invasive breast ductal carcinomas and 42 non-cancerous mammary tissues, including 22 mammary fibroadenomas and 20 paracancerous tissues.Results The positive rates of DLC1 mRNA and protein expression in the breast carcinomas (50% and 57.7%) was significantly lower than that in the non-cancerous mammary tissues (90.5% and 92.9%) (χ~2=17.518 and 10.729,P＜0.01).The expression of DLC1-mRNA was positively related to DLC1protein (r_s=0.379,P＜0.01). The positive rate of Ki-67 expression was 61.5% in the breast carcinomas, but no expression was observed in the all non-cancerous tissues (χ~2=39.186,P＜0.01).Correlation analysis showed that DLC1 expression was negatively correlated with Ki-67 expression (r_s=-0.507,P＜0.01).Conclusions Lower or no expression of DLC1 mRNA and protein may play an important role in the pathogenesis and progression in breast carcinoma. DLC1 may inhibit the proliferation of the breast carcinoma cells,which indicates that it may act as a new molecular marker of breast carcinoma.Combining detection of DLC1 and Ki-67 may be useful parameters for evaluating the biological behaviors of breast carcinoma.

Objective To understand the compliance of antihypertensive drug use in patients with hypertension.Methods A retrospective analysis was conducted among 218 patients with hypertension to understand their drug use compliancy and influencing factors,including side effect of the drugs,drug type,educational level,economic status and drug use length.Results The factors including disease course,drug type,drug use length and drug side effects,the economy status,educational level,awareness of hypertension related knowledge and psychological reaction could significantly influence the compliance of antihypertensive drug use.Among the patients surveyed,86.67％ of them with poor drug use compliance had only an educational level less than senior high school,77.33％ had poor awareness of hypertension related knowledge.Conclusion The antihypertensive drug use compliance in patients with hypertension is directly related to the outcome of the disease in clinical treatment.It is necessary to take effective measures to improve the treatment compliance and maintain normal blood pressure level of the patients.