Objective To develop a quality standard of Citrus limon oils.Methods Limonene in Citrus limon oils was identified by capillary gas chromatography and its content was determined by internal standard method.A polyethylene glycol elastic quartz capillary column (30 m of length,0.32 mm of internal diameter,0.25?m of film thickness) was used,column temperature being 90℃and split ratio being 5:1.Results In the range of 0.0627 mg/mL～4.0136 mg/mL for limonene,an equation was obtained:Y=1.53321X-0.05275,r=0.999 9.The average recovery of limonene was 97.2 % with RSD being 0.6 %.Conclusion The method is stable and reliable,and can provide evi- dences for the development of quality standard of Citrus limon oils.

Objective To study the hemodynamic effects of enhanced external counter pulsation(EECP)on typical coronary artery disease and microcirculation angina.Methods A physiological model of the right dominant coronary artery,including the coronary conduit arteries and coronary microcirculation,was established using lumped parameter models.Pathological conditions,such as one-vessel lesions,three-vessel lesions,and microcirculation angina,were simulated.EECP intervention models were established,and the hemodynamic effects of EECP on pathological models was simulated.Results The simulation results of the coronary physiological model,pathological models,and EECP intervention model established in this study were consistent with experimental data in related literature.EECP improved coronary blood flow in all three pathological conditions.For one-vessel lesions,EECP could not recover the blood flow of left main coronary artery to a normal level after the stenosis rate reached 80%-85%.For three-vessel lesions,EECP treatment could not be used if the stenosis rate in one of the three vessels exceeded 90%.For microcirculation angina,EECP was effective when critical condition myocardial blood flow was>1.03 mL/min·g and coronary flow reserve was>1.64.Conclusions The model of coronary disease under EECP interference established in this study meets expectations,and the obtained simulation data have certain reference values for the clinical application of EECP.