57 school children were immunized with the Japanese encephalitis (JE) veccine produced at NIHE, Hanoi (Nakayama vaccine strain). One year after the third dose, their sera were examined for the presence of antibodies against a JE virus clinical isolate (HNH 693, isolated in North VietNam). The results showed that 100% of the serum samples had high antibody titers against both HNH 693 and Nakayama strains. the antibody titer against 693 was significantly higher than thatagainst Nakayama. Potency testing of 11 batches of NIHE-JE vaccine were carried out using parallely HNH 693 and Nakayama as challenge strains. It showed that all these 11 vaccine batches had high potency against both challenge strains (2.62 with HNH 693 and 2.38 with Nakayama).
Encephalitis, Japanese ; Encephalitis Viruses, Japanese ; ; child

A new minicircle in the mitochondria genome of Leishmania mexicana M379, clone E8 was monitored using different methods as PFGE, hybridization,... The 0,7-kb of the circle was cloned and analysed. It appears to have a Chi-like octomer in the region of replication
Mitochondria ; Leishmania mexicana

A mitochondria minicircle in Leishmania mexicana M379, clone evans E8 was cloned and characterized. The molecules exist as dominant class in the mitochondria genome of the parasiters.
Mitochondria ; Leishmania mexicana

Vnukovo-32 a cell culture rabies is used to study the vaccine strain technology of inactivated culture rabies vaccine production in primary hamster kidney cells. The master and working seeds meet the criteria of rabies vaccine strain titer (5.5 log LD 50 - 7.1 log LD 50/ml) according to WHO standard. During the study, we have used the procedure for inactivated, non concentrated rabies vaccine production of Russia and other countries procedures as references. The result was we constructed the procedure for inactivated, concentrated cell culture rabies vaccine production at laboratory scale.
Rabies Vaccines ; Cells, Laboratories

There is a conservative region of more than 130 bp long in the mitochondria genome of Leishmania mexicana M379 clone E8, and this it is found across many other Leishmania spp. Based on this notion, a pair of PCR primers were designed to amplify the corresponding regions in the minicircles of many other Leishmania species such as L.major, L.donovani. PCR method can be used to detect protozoa in Vietnam
Leishmania ; Polymerase Chain Reaction ; diagnosis

The outer membrane protein HP 1125 of Helicobacter pylori was expressed as a recombinant protein in E.Coli by a short time method for Western and ELISA analysis. The analysis was conducted on more than 100 patients in Vietnam
Helicobacter pylori ; Helicobacter ; Stomach Diseases ; diagnosis ; proteins

Introduction: Rabies is a serious problem in the area of public health in developing countries including Vietnam. The death rate is almost 100%, however rabies can be prevented and preventively treated by vaccine, or a combination between vaccine with rabies resistant serum. The production of Vero cell culture vaccine is becoming a common trend worldwide because of its effective protection and safety. There is a requirement for the domestic production of cell cultured rabies vaccine. \r\n', u'Objectives: To determine the adaptive ability of VNUCOVO - 32 rabies vaccine strain into Vero cell. \r\n', u'Subjects and Method: Rabies vaccine propagation strains the rat\u2019s kidney cells. VNUCOVO - 32 was cultured into Vero cell with different conditions such as pH, temperature, multiplicity of infection (MOl) and time of virus harvests to discover the optimal conditions for virus propagation. \r\n', u'Results: The optimal condition for VNUCOVO - 32 propagation into vero cell with MOl 0.3, pH 7.4 and temperature is 37\xb0C. The highest titer achieves 103,4FFLJ/ml. The best time for virus harvest is 12 - 13 days post inoculation. \r\n', u'Conclusion: VNUCOVO rabies vaccine strain can penetrate and propagate into vera cell. \r\n', u'
Rabies virus ; VNKONO \u2013 32 strain ; Vero cell

OBJECTIVES: To identify trends in percentage body fat (PBF) in adolescents to determine gender-specific classification thresholds for being overweight and obese. METHODS: A cross-sectional study of 899 adolescents (11 to 14.5 years) from Hanoi and Nam Dinh was conducted in Vietnam. PBF, subcutaneous fat and percentage of lean mass were measured directly using OMRON HBF 375 scales to measure bioelectrical impedance. RESULTS: PBF decreased in boys with increasing age (p < 0.001). The difference in PBF between boys and girls, significantly increased with age after 12.5 years (p < 0.001). There was a stronger correlation between PBF and fat content (Pearson's r = 0.860, p < 0.0001) than that between (BMI) and fat content (Pearson's r = 0.521, p < 0.0001). The prevalence of being overweight or obese in girls was similar when determined by PBF or BMI. Hanoi had higher rates of adolescents being overweight or obese compared with Nam Dinh. CONCLUSION: PBF provides a more accurate body assessment for being overweight or obese in adolescents compared with BMI.
Adipose Tissue ; Adolescent ; Body Mass Index ; Classification ; Cross-Sectional Studies ; Electric Impedance ; Female ; Humans ; Obesity ; Overweight ; Prevalence ; Subcutaneous Fat ; Vietnam ; Weights and Measures

OBJECTIVES: To investigate the relationship between dyslipidemia and obesity status among Viet-namese adolescents. METHODS: In this case-control study, 282 adolescents (6–11 years), including 88 obese cases and 194 normal-weight controls, were recruited from a population-based cross-sectional study from two provinces in Vietnam. The anthropometric, blood lipid, and other laboratory test results of the study subjects were analyzed. RESULTS: Obese children tended to have more visceral fat (Pearson's r = 0.795, p < 0.0001) than subcutaneous fat (Pearson's r = 0.754, p < 0.0001), and this difference was associated with an increase in blood triglyceride level (Pearson's r = 0.232, p < 0.05) and a strikingly high rate of hypertriglyceridemia (38.6%). We also found that birth weight and parental body mass index were related to the status of obesity among the study subjects. However, only birth weight was significantly higher in the obese group than in the normal weight group. These findings indicate the effect of prenatal nutrition on childhood obesity. Furthermore, high-birth weight children had a surprisingly high rate of obesity. CONCLUSION: Together, our data suggest that obesity increased the risk for hypertriglyceridemia, which was, at least partially, due to prenatal nutrition.
Adolescent ; Birth Weight ; Body Mass Index ; Case-Control Studies* ; Child ; Cross-Sectional Studies ; Dyslipidemias ; Humans ; Hypertriglyceridemia* ; Intra-Abdominal Fat ; Obesity ; Parents ; Pediatric Obesity* ; Subcutaneous Fat ; Triglycerides ; Vietnam*

In the present study, bioactivity-guided extraction and isolation of the n-hexane fraction of the fruits of Vietnamese Schisandra sphenanthera led to the isolation of five dibenzocyclooctadiene lignans as gomisin N (1), schisandrin C (2), gomisin H (3), gomisin D (4), and gomisin C (5). All the isolates were tested for their inhibition of NO production in LPS-induced RAW 264.7 cells. Among them, compounds 4 and 5 showed weak inhibition of NO production with IC 50 values of 25.0 ± 1.6 and 24.8 ± 2.0 µM, respectively. Compound 1 exhibited NO production inhibition with an IC 50 value of 15.8 ± 2.1 µM, meanwhile, schisandrin C (2) showed the most potent inhibition with an IC 50 value of 8.5 ± 0.5 µM. In addition, compound 2 had a concentrationdependent inhibitory effect on the protein expression of the inflammatory enzymes iNOS and COX-2. Their physicochemical properties and ADMET data were predicted by in silico, indicating favorable drug-like properties as well as low acute oral toxicity. The results suggest that the fruit of S. sphenanthera and its phytochemical constituents might be used as anti-inflammatory agents.