1.Influence of Decoction Time on The Total Count of The Total Free Anthraquinone in Radix et Rhizoma Rhei
Yongtao LIU ; Hong ZHANG ; Zhou HANG
Journal of Zhejiang Chinese Medical University 2006;0(05):-
[Objective]To determine the relation between the total count of the five free anthraquinone(A loe-emodin,Rhein,Emodin,Chrysophanol,Physcion) in Radix et Rhizoma Rhei and the decoction time.[Method]HPLC was used with the chromatographic conditions as follows:mobile phase:methanol-0.1% phosphonic acid solution = 85 :15,detection wave length:254nm,flow rate:1.0 ml/min,column temperature:room temperature.The count of the five free anthraquinone in the decoction taken in every 2 minutes after the solution boiled was determined.[Results]During the 22 minutes after the solution was boiled,the total count took on a ascending tendency,afterwards,it took on a descending tendency.[Conclusion]The biggest number of the total count was obtained 20min after the solution was boiled.
2.The enhancement effect of domperidene on esophagus and lower esophageal sphincter
Lu ZHOU ; Lijian WANG ; Bo YUANG ; Hang ZHANG
Chinese Journal of Digestion 2008;28(9):594-599
Objective To observe the prokinetic effect of domperidone on esophagus and lower esophageal sphincter (LES), and compare its effect with that of mosapride and cisapride. Methods In vivo experiments: forty rats were divided into control, domperidone, mosapride, and eisapride groups. Strain gauges were planted in proximal esophagus, distal esophagus and LES to record the activities of esophagus and LES in conscious rat. In vitro experiments: in the thermostatic muscle bath, the prokinetic effect of domperidone, mosapride, and cisapride on the contractility of rat muscle strips from esophagus body and LES were recorded by tone-transducers. Results In vivo experiments, ① In the interdigestive period of resting conscious rats, only mild contraction activities were recoded in esophagus bodies. In LES, typical interdigestive migrating motor complex (MMC) with phase Ⅰ,Ⅱ,Ⅲ,and Ⅳ was recorded. The contraction amplitude of LES was much greater than esophagus body. ② Domperidone significantly enhanced the contraction of esophagus body and LES. The mean contraction amplitude of proximal esophagus, distal esophagus, and LES increased by 63.24%±7.17%, 75.54%±5.27%, and 85.81%±6.02%, respectively, compared with controls. The prokinetic effect showed a dose-effect relation. Mosapride at the same dosage increased the mean contraction amplitude of proximal esophagus, distal esophagus, and LES by 29. 71%±4.15%, 40.15%±3.30%, and 35.24%±5.36%, respectively, compared with controls. The prokinetic effects of mosapride on esophagus and LES were much less than domperidone. Cisapride at the same dosage increased the mean contraction amplitude of proximal esophagus, distal esophagus, and LES by 59.84%±6.55%, 70.11%±5.62%, and 75.13%± 5.10%, respectively, compared with controls. The prokinetic effects of cisapride were similar as domperidone. In vitro experiments. ① Domperidone perfusion could significantly increase the contraction of esophagus body and LES muscle strips by 87.74%±7.65% and 92.44±7.17%, respectively, compared with Krebs-Ringer (KR) solution perfuslon. Mosapride at the same dosage increased the mean contraction amplitude by 35.42%±5.02% and 31.12%±4.32%, respectively, compared with KR controls. The prokinetic effects of mosapride were much less than domperidone. Cisapride of the same dosage showed a similar prokinetic effect as domperidone. ② Atropine and tetrodotoxin could block the prokinetic effects of domperidone on esophagus and LES. Conclusions Domperidone can significantly enhance the esophagus body contraction and LES motility. The effects of domperidone are similar as cisapride and much greater than mosapride. The prokinetic effects of domperidone on esophagus and LES are not only through well-known dopaminergic receptor blockade, but also through the cholinergic nerves of the enteric nervous system.
4.Composite glandular-neuroendocrine carcinoma in gastric cardia: report of a case.
Zhang-lei ZHOU ; Xin-hua ZHANG ; Hang-bo ZHOU ; Zhong-qiu WANG ; Qun-li SHI
Chinese Journal of Pathology 2009;38(11):779-780
Adenocarcinoma
;
metabolism
;
pathology
;
surgery
;
ultrastructure
;
Aged
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Carcinoembryonic Antigen
;
metabolism
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Carcinoma, Neuroendocrine
;
metabolism
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pathology
;
surgery
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ultrastructure
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Cardia
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Humans
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Ki-67 Antigen
;
metabolism
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Male
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Microscopy, Electron, Transmission
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Stomach Neoplasms
;
metabolism
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pathology
;
surgery
;
ultrastructure
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Synaptophysin
;
metabolism
5.Pituitary carcinoma: report of a case.
Jing ZHOU ; Nan-yun LI ; Zhi-qiang ZHANG ; Chi-yuan MA ; Bo YU ; Hang-bo ZHOU
Chinese Journal of Pathology 2013;42(2):123-125
Adenoma
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pathology
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Brain Neoplasms
;
secondary
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Chromogranin A
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metabolism
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Diagnosis, Differential
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Follow-Up Studies
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Humans
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Magnetic Resonance Imaging
;
Male
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Middle Aged
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Neoplasm Recurrence, Local
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Pituitary Neoplasms
;
diagnosis
;
metabolism
;
pathology
;
surgery
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Reoperation
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Synaptophysin
;
metabolism
;
Temporal Lobe
;
pathology
7.Analysis of mutations in IgVH genes in diffuse large B-cell lymphomas.
Yun LIANG ; Ren ZHOU ; Wei ZHANG ; Huan-lan ZHANG ; Hang-di XU ; Zheng-rong MAO
Chinese Journal of Pathology 2007;36(9):625-626
Base Sequence
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DNA, Neoplasm
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genetics
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DNA-Binding Proteins
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metabolism
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Gene Rearrangement, B-Lymphocyte, Heavy Chain
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Genes, Immunoglobulin Heavy Chain
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Humans
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Immunoglobulin Heavy Chains
;
genetics
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Interferon Regulatory Factors
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metabolism
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Lymphoma, Large B-Cell, Diffuse
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genetics
;
metabolism
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Molecular Sequence Data
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Neprilysin
;
metabolism
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Proto-Oncogene Proteins c-bcl-6
8.Study of association between neutrophil extracellular trap and interstitial lung disease in dermatomyositis patients
Sigong ZHANG ; Xiaolan TIAN ; Yinli ZHANG ; Kanbo YANG ; Hang ZHOU ; Guochun WANG ; Xin LU
Chinese Journal of Rheumatology 2013;17(12):796-799,后插1
Objective This study was focused on the association between neutrophil extracellular traps (NETs) and interstitial lung disease (ILD) in patients with dermatomyositis (DM).Methods Thirty six patients who satisfied the Bohan & Peter criteria for DM were recruited to this study,among whom 19 were complicated with ILD.Forty seven age and sex matched healthy Chinese volunteers were selected to be control subjects.The plasma samples of these patients were tested for the formation and degradation of NETs.Results DM plasma induced more NETs formation than control plasma did [(246±93) RFUs vs (192±53) RFUs,P=0.002].Compared to control,DM plasma exhibited a signficantry decreased ability to degrade NETs.Further mere,compared with DM patients without ILD (DMNL),DM patients with ILD (DML) could not degrade NETs completely [(83±13)% vs (59±21)%,P<0.01].All four DM patients with subacute ILD exhibited a significantly lower ability to degrade NETs than patients with chronic or asymptomatic ILD [(36±14)% vs (65±19)%,P=0.0139].Conclusion These data show that more NETs formation is induced by plasma and DML fails to completely degrade NETs.These suggest that NETs may play a role in the pathogenesis of DM and DM-associated ILD.
9.Space-occupying lesion in left upper lobe of lung.
Xin-hua ZHANG ; Qun-li SHI ; Zhi-yi ZHOU ; Wen-bin HUANG ; Hang-bo ZHOU ; Xiao-jun ZHOU
Chinese Journal of Pathology 2006;35(7):432-433
Adult
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Antibodies, Monoclonal
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metabolism
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Diagnosis, Differential
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Follow-Up Studies
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Humans
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Immunohistochemistry
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Lung Neoplasms
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metabolism
;
pathology
;
surgery
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Male
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MyoD Protein
;
metabolism
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Pneumonectomy
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Pulmonary Veins
;
metabolism
;
pathology
;
surgery
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S100 Proteins
;
metabolism
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Sarcoma, Alveolar Soft Part
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metabolism
;
pathology
;
surgery
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Vascular Neoplasms
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metabolism
;
pathology
;
surgery
10.Influence of tranilast on the cyclosporine A-induced epithelial-to-mesenchymal transition in human renal tubular epithelial cells
Qiangping ZHOU ; Dongliang XU ; Ting ZHANG ; Qiang LU ; Zhijian HANG ; Zhengquan XU ; Yuangeng SUI ; Min GU
Chinese Journal of Organ Transplantation 2011;32(4):235-239
Objective To study the effect of tranilast on cyclosporine A (CsA)-induced epithelial-to-mesenchymal transition in human renal tubular epithelial cells, and investigate the mechanism of its antifibrotic effect. Methods Cultured HK-2 cells were divided into four groups: (1)In the control group, cells were treated without any medicine; (2) The cell were treated with CsA (4. 2μmol/L) for 72 h; (3) The cells were treated with a combination of CsA (4. 2 μmol/L) and tranilast (100μmol/L); (4) The cells were treated with tranilast (100 μmol/L) alone for 72 h.Morphological changes of the cells were assessed by phase-contrast microscopy. The immunofluorescence and Western blotting were adopted to detect the expression of E-cadherin, α-SMA and OPN mRNA and proteins respectively. Results Tranilast could markedly ameliorate the morphological changes of HK-2 cells stimulated by CsA. The irmmunofluorescence staining revealed the expression of E-cadherin was markedly decreased in HK-2 cells stimulated with CsA for 72 as compared with the control group, while the expression of α-SMA and OPN was significantly higher in CsA group than the control group. The expression of E-cadherin in the CsA + Tranilast group was higher than the CsA group, while the expression of α-SMA and OPN in the CsA + Tranilast group was lower than the CsA group. Western blotting showed that protein expression level of E-cadherin in CsA group was dramatically lower than that in the control group (P<0. 05), while that of α-SMA and OPN in CsA group was significantly higher than in the control group (P<0.05). The protein expression level of E-cadherin in HK-2 cells in the CsA + Tranilast group was markedly higher than in the CsA group (P<0.05), and that of α-SMA and OPN in CsA + Tranilast group was significantly lower than in the CsA group (P<0. 05). Conclusion Tranilast can block the CsA-induced epithelialto-mesenchymal transition in HK-2 cells probably by suppressing the expression of OPN.