Objective To analyze the characteristics of of organochlorine pesticides(OCPs) residues in the soil of the suburbs of Guangzhou.Methods In Baiyun,Conghua,Zengcheng,Panyu,Nansha,surface soil(0-20 cm) samples of vegetable fields,orchard and paddy soil were collected,soil profile(20-40cm,40-60cm) of Baiyun and Panyu were also collected,in Aug,2008.Gas chromatography-electron capture detector method(GC-ECD)was used to determine 17 kinds of organochlorine pesticides.Results The detection rate of 17 kinds of organochlorine pesticides was 90.59% in topsoil,the residue level was 4.19-39.89 ng/g,and the detection rate in profiles soil was 76.70 %,the residue level was 2.35-91.26 ng/g.OCPs residues were mainly HCHs and DDTs.Conclusion Detection rate of organochlorine pesticides is high in soil in the investigated soil and in the 20-40 cm profiles of Baiyun and Panyu,the level of OCPs exceeds the related standard limit,which may have adverse effects on soil environment.

BACKGROUND:Previous studies on immunosuppression and anti-rejection after organ transplantation mainly focused on effects of T lymphocytes-mediated immune response and immunosuppressive agents on T lymphocytes. Effects of dendritic cel s were unclear. The manifestation and mechanism of immunosuppressive agent effects on dendritic cel s are not identical. OBJECTIVE:To compare the effects of different immunosuppressive agents on expression and function of costimulatory molecules of dendritic cel s, and to explore the mechanism of action of immunosuppressive agents. METHODS:20μg/L rapamycin, 0.04 mg/L mycophenolate, 10μg/L tacrolimus and 1 mg/L cyclosporine A were separately added during bone marrow cel s of C57BL/6 mice were differentiated into dendritic cel s. RESULTS AND CONCLUSION:Flow cytometry results revealed that CD40 expression in each group:rapamycin0.05). One-way mixed lymphocyte reaction results displayed dendritic cel costimulatory T cel proliferation in each group:rapamycin

Objective To explore the clinical application of percutaneous endoscopic gastrostomy in gastroin- testinal nutrition among critically ill patients.Methods Twenty-two patients were undertaken percutaneous endo- scopic gastrostomy under the lead of gastroscope.The puncturatio site was located in anterior wall of stomach.Results All the procedures were performed successfully for one time.Intraoperational blood pressure was very steady.At the same time,intraoperational SpO_2 of the patients all exceeced 97%.The bleeding amount and operation time were respectively (3.8?1.9) ml and (15.5?2.3) min.Severe complications such as gastrostoma,gastrocolic fistula, pneumoperitoneum,refluxing or aspiration of gastric juice,inhalant pneumonia didn't occur after the operation.Con- clusion Percutaneous endoscopic gastrostomy was safe and feasible among critically ill patients who needed gastroin- testinal nutrition because of its slight injury,little bleeding and shorter operational time.

The study was purposed to explore the effect and mechanisms of decitabine and/or Trichostatin A (TSA) on SKM-1 cells in vitro. The effect of decitabine and/or TSA on proliferation of SKM-1cells was analyzed with trypan blue exclusion; the differentiation of SKM-1 cells was detected by nitro-blue tetrazolium (NBT) reduction and flow cytometry; the apoptosis of cells was measured by Annexin V-FITC; the mRNA expression of Fas, survivin and P15(INK4B) in cells treated with decitabine and/or TSA was evaluated by RT-PCR. The results showed that decitabine and/or TSA were capable of inhibiting SKM-1 cell growth and promoting cell differentiation; they stimulated the expression of CD14 and CD11b and inhibited HLA-DR expression; meanwhile and decitabine or/and TSA could induce cell apoptosis, up-regulate mRNA expression of Fas and P15(INK4B), and down-regulate survivin mRNA expression. It is concluded that decitabine can induce apoptosis/differentiation of SKM-1 cells, whose mechanisms may related to the expression of Fas, survivin and P15(INK4B). Decitabine has the synergistic effect with TSA.
Antimetabolites, Antineoplastic ; pharmacology ; Apoptosis ; drug effects ; Azacitidine ; analogs & derivatives ; pharmacology ; Cell Differentiation ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Drug Synergism ; Humans ; Hydroxamic Acids ; pharmacology ; Inhibitor of Apoptosis Proteins ; Microtubule-Associated Proteins ; genetics ; metabolism ; Myelodysplastic Syndromes ; pathology ; fas Receptor ; genetics ; metabolism

Anti-tumor traditional Chinese medicine has a long history of clinic application, in which the star molecules have always been the hotspot of modern drug research, but they are limited by the solubility, stability, targeting, bioactivity or toxicity of the monomer components of traditional Chinese medicine anti-tumor star molecules and other pharmacokinetic problems, which hinders the traditional Chinese medicine anti-tumor star molecules for further clinical translation and application. Currently, the nanosystems prepared by supramolecular technologies such as molecular self-assembly and nanomaterial encapsulation have broader application prospects in improving the anti-tumor effect of active components of traditional Chinese medicine, which has attracted extensive attention from scholars at home and abroad. In this paper, we systematically review the research progress in preparation of supramolecular nano-systems from anti-tumor star molecule of traditional Chinese medicine, and summarize the two major categories and ten small classes of carrier-free and carrier-based supramolecular nanosystems and their research cases, and the future development direction is put forward. The purpose of this paper is to provide reference for the research and clinical transformation of using supramolecular technology to improve the clinical application of anti-tumor star molecule of traditional Chinese medicine.

Objective To explore the acute toxicity effect of CKLF1-C19,a polypeptide of chemokine-like factor 1(CKLF1),on the KM mice. Methods A total of 40 KM mice,half male and half female,were randomly divided into 2 groups. The mice in the experimental group were injected with CKLF1-C19 at a dose of 25 mL/kg(100 μg/mL,1 mg/mL and 10 mg/mL)through the tail vein,and those in the control group received an equal volume of sterile saline solution. Changes in the body weight of the mice were recorded the day after treatment, and the general conditions of mice in the experimental group were observed closely and compared with the normal group. Then blood samples were taken from the abdominal aorta to measure liver and kidney function. Tissue samples of liver, kidney, spleen and lung were taken for histopathological examination by HE staining. Results In the maximum tolerance test,the mice of the two groups were in good condition, and their body weight was increased gradually, without significant difference between the experimental group and the control group(P > 0.05). There was no death within 14 days. The blood biochemical indexes of liver and kidney function showed no significant differences between the two groups(P > 0.05). The gross appearances of heart, liver,kidney,spleen and lung were normal in the two mouse groups, and the pathological examination with HE staining showed normal clear structure with no obvious changes in these organs of each group. Conclusions Our results demonstrate that CKLF1-C19 has no acute toxicity effect on mice.

Adult ; Embolism, Paradoxical ; diagnosis ; therapy ; Humans ; Male ; Pulmonary Embolism ; diagnosis ; therapy

Objective To observe the clinical efficacy and safety of atorvastatin calcium tablets in the treatment of nontraumatic subdural hematoma.Methods A total of 52 patients with nontraumatic subdural hematoma were randomly divided into control group and treatment group with 26 cases per group.Control group was given the treatment of drilling and drainage for 2-3 days.Treatment group received atorvastatin calcium 20 mg,qd,oral for 28 days,on the basis of control group.The clinical efficacy,serum superoxide dismutase (SOD),malondialdehyde (MDA),recurrence rate,and adverse drug reaction were compared between the two groups.Results After treatment,the total effective rates in treatment and control groups were 84.62％ (22/26 cases) and 57.69％ (15/26 cases) with significant difference (P ＜0.05).After treatment,the SOD in control and treatment groups were (115.29 ±13.34),(128.39±14.29) U·mL-1;MDA were (19.83 ±2.43),(15.40 ± 1.67) nmol · mL-1;recurrence rates were 26.92％ (7/26cases) and 0 (0/26 cases),the differences were statistically significant (all P ＜ 0.05).The adverse drug reactions in treatment group were based on increased hematoma volume and infection,which in control group were based on intracranial emphysema,increased hematoma volume,infection,abnormal liver function and brain damage.The incidences of adverse drug reactions in treatment and control groups were 7.69％ and 34.62％ with significant difference (P ＜ 0.05).Conclusion Atorvastatin calcium tablets have a definitive clinical efficacy and safety in the treatment of nontraumatic subdural hematoma.

Objective:To screen and identify the differentially expressed proteins in the serum ofα-fetoprotein (AFP)-negative patients with liver cancer and AFP-negative patients with liver cirrhosis.Methods:Six patients with AFP-negative liver cancer and 6 patients with AFP-negative liver cirrhosis were enrolled.The low abundance proteins in serum samples from each group were isolated by MARS system.Then the differentially expressed proteins between the two groups were screened by two-di-mensional fluorescence difference gel electrophoresis (2D-DIGE)technology.Furthermore,Western blotting was used to verify the differentially expressed proteins.Results:Totally,29 differentially expressed proteins have been identified by 2D-DIGE technology.Among those proteins,22 proteins were over-expressed in the serum from AFP-negative liver cancer patients, while 7 proteins were down-expressed.These differential proteins included kininogen 1 ,tetranectin,lumican,complement 1 R and zinc-alpha-2-glycoprotein (ZAGP).By Western blotting,it was confirmed that the expression of ZAGP in patients with AFP-negative liver cancer was significantly lower than that in patients with AFP-negative liver cirrhosis (P <0.05),which was in accordance with the result of DIGE.Conclusions:A series of differentially expressed proteins were identified in the serum from AFP-negative patients with liver cirrhosis or liver cancer by using proteomic technology.These proteins might be used as molecular markers for the early diagnosis and assistant diagnosis of AFP-negative patients with liver cancer.

Objective To analyze the status of community nursing personnel training and explore its reform direction.Methods Totals of 160 community health service institutions in 11 cities in Zhejiang province were investigated by convenience sampling.The content validity of the modified questionnaire was 0.9,and the reliability Cronbach's α coefficient was 0.87.Results One hundred and sixty questionnaires were issued and 150 questionnaires were responsive with an effective response rate of 93.8％,including 119 community health service centers and 31 community health service station.Most community organizations (57.1％) thought that current personnel training program of nursing could not satisfy the requirements of practical community nursing.The shortcomings were as follows:1) insufficient health and recovery knowledge (54.7％) ; 2) insufficient humanity knowledge,such as psychology and law (45.2％) ; 3) insufficient knowledge about epidemic disease and preventive medicine (42.6％) ; 4) insufficient class hour on community nursing (40.0％) ; 5) insufficient knowledge on nursing research (18.7％).Most community organizations thought it was necessary for the college to set up community nursing class (54.8％).The courses they suggested were:preventive medicine,recovery medicine,health education,psychology,epidemic medicine,medicine and society.The core skills of the community nurses should include the skill to communicate with patients,the ability of disease systematic nursing,independent analysis and problem solving.Conclnsions Current college education can not fully meet the requirements of community nursing personnel training.College should set up special class on community nursing,revise the courses oriented towards community nursing,and emphasize the core skills of community nursing.