Objective:To compare the ocular tissue concentrations and pharmacokinetic parameters in rabbit's eyes after topically and subconjunctivally administrated with levofloxacin (LVFX).Methods:The concentrations of LVFX in the cornea,aqueous humor,iris-ciliary body,lens and vitreous at different times were measured by high performance liquid chromatography after 0.3% LVFX were topically and subconjunctivally administrated into rabbits.Results:The penetration of LVFX into the cornea,iris-ciliary body,lens and vitreous after subconjunctival injection was significantly higher than that of topically applied one( P

Objective To investigate the safety of inhalation of isoflurane (ISO) or sevoflurane (SEVO) mixed with nitric oxide (NO) during mechanical ventilation.Methods Thirty-six healthy piglets of both sexes weighing 7-11 kg were randomly allocated to one of 6 groups ( n = 6 each): (1) control group was mechanically ventilated with O2; (2) NO group inhaled 20 ppm NO; (3) ISO group inhaled 1.3 MAC isoflurane; (4) ISO + NO group 1.3 MAC isoflurane + 20 ppm NO; (5) SEVO group inhaled 1.3 MAC sevoflurane and (6) SEVO + NO group inhaled 1.3 MAC sevoflurane + 20 ppm NO. The animals were mechanically ventilated with IPPV (VT 10 ml?kg-1 , RR 30-40 bpm, I: E 1:2) for 4 h in the all 6 groups. The animals were premedicated with atropine 0.02 mg?kg-1 i.m. . The Ⅳ line was established for fluid and drug administration. An additional dose of ketamine 10 mg?kg-1 was given i.v. before tracheostomy. 4F S-G catheter was placed in pulmonary artery via right internal jugular vein for hemodynamic monitoring. Femoral artery was cannulated for BP monitoring and collection of artrerial blood samples. MAP, HR, CVP, right ventricular pressure (RVP), PCWP, MPAP and total compliance of respiratory system (Crs), Paw, VT and PET CO2 were recorded before (T0 ) and at 1, 2, 3, 4 h of ventilation (T1-4). Blood samples were taken at T0 , T2 and T4 for determination of Met Hb and NO2- /NO3- . The animals were killed at the end of 4 h mechanical ventilation and the lungs were removed for determination of wet/dry(W/D) lung weight ratio, broncho-alveolar lavage fluid (BALF) and microscopic examination. BALF was collected for determination of surface tension and concentrations of saturated phosphate (DSPC) , total phosphate (TPL) total protein (TP) and white cell count. Results Crs was significantly decreased at the end of ventilation (T4 ) as compared with the baseline (T0) in group 3,4,5 and 6, while there was no significant change in Crs in group 1 and 2. DSPC/TP was significantly lower in group 3,4,5 and 6 than in group 1 ( P 0.05 ) . Conclusion 1.3 MAC isoflurane or sevoflurane mixed with 20 ppm NO can be used safely during mechanical ventilation.

OBJECTIVE: To introduce material science characteristics and clinical application of bone plate. METHODS: The first author retrieved PubMed Database (http://www.ncbi.nlm.nih.gov/), China National Knowledge Infrastructure (http://www.cnki.net/) and Wanfang Database (http://www.wanfangdata.com.cn) for articles addressing material science characteristics and clinical application of bone plate published from 2000 to 2009. The key words included "bone plate, material science characteristics, biocompatibility, clinical application". Testing articles with reliable argument and evidence as well as close topic were included, and simultaneously, non-original articles were excluded. Ultimately, 25 articles were included. We arranged and analyzed material science characteristics, clinical application, advantages and disadvantages of bone plate. RESULTS: From the material science characteristics, the elastic modulus of bone plate should be close to skeleton so as to reduce stress occlusion effects. In clinical application, we should pad attention to the bonding of biological fixation and mechanical fixation to improve plate structure, resulting in decrease in contacting area of plate and skeleton to diminish or prevent osteoporosis in local regions. CONCLUSION: Presently, bone plate materials mainly contain stainless steel, cobalt alloy and titanium alloy in clinic with their advantages and disadvantages. Among them, medical titanium alloy is an ideal in vivo implant. In clinical application, less invasive stabilization plate provides a powerful technique security for early recovery of motor function following fracture, and shows a spacious application prospect.

Objective: To compare cumulative dose technique with single dose technique for determining dose-re sponse relationship of rocuronium during balanced anesthesia. Method: Thirty-four ASA grade Ⅰ-Ⅱ patients, aged 23- 65 years, undergoing elective surgery,were randomly allocated to single dose group(n=24) or cumulative dose group (n=10). Single dose group was further divided into 4 subgroups receiving 150,200,250 or 300?g/kg of rocuronium re spectively. In cumulative dose group, the initial dose of 150 or 200 ?g/kg rocuronium was followed by a second dose de termined individually to produce about 90% neuromuscular block. The ulnar nerve was stimulated at the wrist to mea sure the accelography of thumb adduction. The relationship between probit-transformed depression of first twitch height of TOF and the logarithm dose of rocuronium was analysed using linear regression. Result: ED_50 and ED_95 of rocuronium were 193.6?69.1 and 367.2?72. 7?g/kg respectively in single dose group, and 213.0?55.1 and 418.5? 61.7?g/kg respectively in cumulative dose group. No statistically significant differences were found in the slope,inter cept,ED_50 and ED_95 values between two groups. Conclusion: The two-dose cumulative technique can be used as effec tively as traditional single dose technique in determining the dose-response relationship of rocuronium.

Objective To investigate the efficacy of bilevel positive airway pressure mechanical ventilation (BiPAP) in the patients of acute organophosphorus pesticides poisoning (AOPP) with respiratory failure. Methods 44 AOPP patients with respiratort failure were randomly divided into experimental group (n = 22) and control group (n = 22). The routine trentment was given to both groups, additional BiPAP assisted ventilation was given to the treatment group. Blood gas,heart rate and respiratory rate were detected before and 2 h,24 h,48 h after treatment, and the rote of intubatinn,mortality,duration and cost of hospitalization were compared between two groups. Results Heart rate,PaO2 was significantly increased and PaCO2 decreased in BiPAP ventilation (P <0. 05). The mortality,intubatien rate and cost of hospitalization were significantly decreased in BiPAP treatment group (P < 0. 05). Conclusion The BiPAP ventilation is certainly effective to acute organophosphorus pesticides poisoning with respiratory failure. It could obviously improve the clinical symptoms and arterial blood gas analysis, and could reduce the mortaiity, rate of intubotion,cost of hospitalization of patients of acute organophosphoms pesticides poisoning with respiratory failure.

Objective To explore and analyze the clinical characteristics and treatment strategy of severe complications caused by childrenˊs foreign bodies in esophagus.Methods The clinical data of 71 ca-ses of esophageal foreign bodies in children with ENT admitted to our hospital were analyzed retrospectively from Jan 2009 to Dec 2014.Results Esophageal foreign bodies in children with 71 cases,of whom 42 males and 29 females;aged from 2 months to 14 years old,highest incidence of 1 to 3 years old.Foreign bodies in esophagus lasted 2 h to 15 d.Ingestion of metallic foreign bodies in 43 cases,12 cases of plant-based foreign body,9 cases of animal foreign bodies,7 cases of chemical foreign bodies.Foreign bodies located in the upper esophageal was 51 cases,9 cases of the middle and 7 cases of the under section,3 cases of esophageal en-trance,1 case of falling into the stomach.In children with 71 cases,9 cases had complications,69 cases were cured,improved in two cases,and the cure rate was 97.2%,the average healing time was 4 d.Conclusion Early diagnosis and treatment,avoid esophageal perforation are the key to avoid the emergence of the compli-cations in children with esophageal foreign bodies,timely and accurate treatment of esophageal perforation and other complications are the effective measures to improve the cure rate.

desflurane. The one of hepatotoxic mechanism of inhatational anesthetics may be to inhibit the proliferation and albumin secretion.

Ⅳ, SVR and PVR decreased markedly at the same time MERO 2 and MDO 2/MVO 2 dod not change significantly but MVO 2/MDO 2 showed a tendency of decreasing after the inotropics administration compared with the control group Electronic microscopy examinations: 30min after the inotropics were stoped, the myocardial ultrastructure of ischemic area did not improved significantly in group Ⅱ and Ⅲversus the group Ⅰ, but in group Ⅵ, Ⅴ and Ⅳ, the interstitial and intracellular edema reduced markedly, the capillary stasis vanished, and very few vacuole could be seen in mitochondria Conclusions 1 The response of stunned myocardium to ? agonists decreases 2 Inotropic can restore regional work by restoring mechanical synchrony and improving energy efficiency, the effects of combined inotropics are better than the inotropics given alone in myocardial stunning

Objective To evaluate the roles of K ATP channels in desflurane-induced myocardial protection from anoxia/reoxygenation injury Methods Primary cultured rat myocardial cells were randomly allocated to four groups: control group(A): without any treatment; anoxia/reoxygenation group(B): reoxygenation of 1 h following anoxia of 2 h; desflurane preconditioning group(C): 20 min of 9% desflurane preconditioning followed by 10 min washout before anoxia/reoxygenation and K ATP channel blocker group(D): adding glybenclamide at final concentration of 12?g/ml to culture medium 10 min before the same procedures as group C The activities of lactic dehydrogenase (LDH) and creatine kinase (CK), rates of cell viability and apoptosis, contents of cellular malondiadehyde (MDA) and adenosine triphosphate (ATP), and intracellular free calcium concentration were measured Results Compared with control group, anoxia/reoxygenation caused great increases of levels of LDH, CK, apoptosis and MDA ,and decreases of ATP and cell viability (P