Suming up 1883 hemoglobin electrophoresis patient sample, regardless of age, suspected thalassemia and or Hb disease distributed as following: *-thal: 59.65% including-thalhomozygous: 10.43% -halhomozygous 1.20%, -thal/HbE 29.56% trait 18.46%. *HbE (HbE only and -thal/HbE) 39.66% *  thal (HbH and HbH/Hb CS) 27.25%. *HbCS: 7.47%. *  and HPFH heterozygous: 2.96%. From these results, it is necessary to up grade the test of quantification of HbA2 and simultanous develop plan of mass screening for thalasemia trait early to identify persons who are at risk of having a child with a severe disease.
Blood ; Hematologic Diseases

The HLA loci of the human Major Histocompatility Complex (MHC) located on the short arm of human chromosome 6 encoded two distinct classes of highly polymorphic cell surface molecules. These play a central role in the regulation of the immune system, in transplantation biology as well as in susceptibility to a number of diseases including auto immune disorders. The PCR-based reverse line line-blot assay that has been performed in Cord Blood Bank in HCM Haematology and Blood Transfussion Centre (CBB, HCM HBTC) has many advantages over the more traditional technique used for HLA typing (serological and MLR): -Whole blood is used.- Not necessary to isolate live class I cells Reagents are prepared synthetically.
Phenotype ; HLA Antigens ; Polymerase Chain Reaction

Fifty umbilical cord blood samples have been collected for investigation of hematological index. Values of leucocyte, granulocyte, lymphocyte, monocytes, red corpuscles and reticulocytes have been harvested from Cell Dyn 3,500. The Hb electrophoresis by Bechman seperated well HbF from HbA. Most of our values are similar to Wintrobe’s but for granulocyte, red cell, hemoglobin and volume packed red cell are signigicantly lower if comparing to Wintrobe’s.
Fetal Blood ; Hematology

The relationship between the relative amounts of HbH and the proportions of RBC with inclusion bodies (IBs) as shown that almost straight, getting a correlation coefficient of 0.86, the regression line cuts the x- axis of RBC with lbs at 19%, indicating that demonstration of RBC with IBs is more sensitive in detecting HbH than electrophoresis. Strickingly the mean level of both HbH and percentage of RBC with lbs were significantly higher in a- than 1/HbCS than in a- than 1/ a -than2. These results led us to conclude that examination of the intraerythrocytic inclusion bodies is able to be used as a “probe” technique in unequiped laboratory for detecting HbH disease.
Erythrocytes ; Hemoglobin H