Objective　To investigate dynamic changes of the p53 gene and its protein during occurrence and metastasis of colorectal cancer and explore the relationship between p53 mutation and survival of the patients. Methods　p53 gene (exon 5-9) was examined by PCR, denaturing gradient gel electrophoresis (DGGE) and automated sequencing. Results　p53 alterations were found in exons 5 through 9 in 26 of the 41 patients (63%). Of the 26 patients, 6 had the alterations in the liver metastatic lesions but not in the original colorectal lesion. The other 20 had the alterations in both of the primary colorectal and hepatic metastatic lesions. Meanwhile, an additional mutation in the metastatic lesions was found in 3 cases. The analysis about the survival revealed that the patients with mutant p53 in the metastatic lesions had a longer survival as compared with those with wild type p53. Conclusion　In the process of liver metastasis of the colorectal cancer, p53 mutations mainly start in the primary colorectal lesion and then is kept and brought into the liver. It also might start from metastatic lesion in a few cases. For the patients who had liver metastasis of colorectal cancer and underwent hepatectomy, the survival is longer in mutant p53 group than in wild type p53 group.

Objective To evaluate the effect and adverse effect of combination chemotherapy with xeloda and oxaliplatin in advanced colorectal cancer. Methods 25 patients were treated with oral xeloda 1 250mg/m~2, administered twice daily from day 1 to day 14, and oxaliplatin 130mg/m~2/d iv on day 1.The regime was repeated every 21 days for at least 2 consecutive cycles. Result The results of evaluation of effectiveness of the reginme were as follow: 0 CR,12 PR,10 SD and 3 PD. The overall effective rate was 48%(12/25), the effective rate for patients who received the treatment primarily was 100%(3/3), and that of the patients who received the treatment for the secona time was 41%(9/22).Xeloda combined with oxaliplatin was well tolerated. The most common treatment-related adverse events with hand-foot syndrome in 40% (10/25) of patients, skin pigmentation in 68% (17/25), anorexia in 40% (10/25) of patients. Suppression of hematopoiesis, neurotoxicity, nausea and vomiting were mild. Conclusion The combined chemo-therapy with xeloda and oxaliplatin has a high therapeutic response with acceptable toxicity in patients with advanced colorectal cancer, and which was convenient to administer, with definite efficacy, and it could be extensively used, especially in elderly patients and outpatients.

Asphyxia ; etiology ; Child ; Dysostoses ; complications ; Female ; Humans ; Osteochondrodysplasias ; Thorax ; abnormalities

Animals ; Berberine ; therapeutic use ; Diabetic Nephropathies ; drug therapy ; Humans

BACKGROUND: In recent years, epidemiological studies have found that the saturation of transferring or the increased level of serum ferritin are associated with the attacks of cancer, coronary heart disease, diabetes mellitus, Parkinson disease, liver disease and the diseases of immune system. Therefore, it is suggested that the intake of excessive iron may cause adverse influence on the healthy of human body.OBJECTIVE: To establish high-iron model in mice by using full-rate diet pellets by adding regular quantitative intraperitoneal injection of iron dextran, and observe the iron levels in vivo and the changes of organ coefficients.DESIGN: A comparative observation.SETTING: Department of Nutrition and Food Hygiene, School of Public Health and Tropic Medicine, Southern Medical University.MATERIALS: The experiment was carried out in the laboratory of the Department of Nutrition and Food Hygiene, School of Public Health and Tropic Medicine, Southern Medical University in May 2006. Forty Kunming mice of SPF grade, 20 males and 20 females, weighing 18-22 g, were provided by the Animal Experimental Center of Southern Medical University. The mice were randomly divided into control group (n =10) and high-iron model group (n =30) by introperitoneal injection of saline and iron dextran respectively, and the latter group was subdivided into low, middle and high-dosage groups (6.25, 12.5 and 25 g/L) respectively, 10 mice in each group. Full-rate diet pellets (iron content was 370 mg/kg)were purchased from the Animal Experimental Center of Southern Medical University. Iron dextran reagent (norm: 2 mL containing 50 mg iron) was the product of Zhejiang Ruian Pharmaceutical Factory (certification number: H33021758).The kits of superoxide dismutase (SOD) and maldondialdehyde (MDA) were provided by the Nanjing Jiancheng Bioengineering Institute.METHODS: Mice in each group were raised in plastic stainless steel cages respectively at (23±3) ℃, and they were free to the access of food and deionized water. Mice in the low, middle and high-dosage group were treated with intraperitoneal injection of iron dextran once every other day, 0.8 mL for each time, whereas iron dextran was replaced by saline in the control group, all the mice were treated for 6 weeks, and their nutritionol conditions were observed. All the mice were killed at the end of the 6th week. The iron contents in organs of heart, liver, spleen, lung and kidney, and serum were determined with atomic absorption spectrophotometer and automatic biochemical analyzer respectively; Pathohistological examination of organs were performed; The organ coefficients of liver and spleen were calculated; MDA content and SOD activity in serum were determined.MAIN OUTCOME MEASURES: General conditions of mice in each group; Iron contents in organs and iron concentration in serum; Organ coefficients of liver and spleen; MDA content and SOD activity in serum; Pathological changes.RESULTS: In the high-iron model group, the body figures of the mice were changed, body masses were obviously decreased. The iron contents in organs and serum of mice in the high-iron model group were all obviously increased as compared with those in the control group (t =5.841, P ＜ 0.01), the organ coefficients of liver and spleen were also markedly increased (t =5.841, P ＜ 0.01), which were all in a dosage-dependent manner. The MDA content in serum was obviously increased (t =5.841, P ＜ 0.01) whereas the SOD activity was obviously decreased (t =12.924, P ＜ 0.01) as compared with those in the control group. The pathohistological examination under light microscope showed that there were pathological damages of different degree occurred in the tissue and cells and cell degeneration was observed,which affected the normal physiological function of cells.CONCLUSION: High-iron mice models can be successfully established by the intraperitoneal injection of iron dextran.The storage of excessive iron in vivo will result in the organic damages.

BACKGROUND: Iron deficiency anemia (IDA) is one of the highest incidence nutritional-deficiency diseases all over the world; especially infants and children are the main group. IDA presently becomes one of the most important nutritional problems to be solved.OBJECTIVE: To observe the effect of chocolate carrier and orange juice on recovery of IDA model rats.DESIGN: Randomized controlled animal study.SETTING: Laboratory of Nutrient and Food Hygiene, School of Public Health and Tropical Medicine, Southern Medical University.MATERIALS: The experiment was carried out in the Laboratory of Nutri ent and Food Hygiene, School of Public Health And Tropiacal Medicine, Southern Medical University from March to June 2006. A total of 60 healthy SD rats of clean grade were provided by Animal Center of Southern Medical University (certification: 2002-009 2005A047). METHODS: ① Establishment of IDA models: Among them, 20 rats of half genders were randomly selected toregard as control group, and other 40 were regarded as model group. Rats in control group were fed with rou tinefeed and drank freely. Rats in model group were fed with AOAC-modi fied low-dosage iron feeds to establish IDA models by blooding at caudal vein. Three weeks later, average concentration of ferrohemoglobin in model group was decreased to about 90 g/L, and this suggested that model estab lishment was successful. Ten rats of half genders in each group were ran domly sacrificed. Pre-experiment and 3 weeks of post-experiment, rats were weighed to measure concentration of ferrohemoglobin with hemoglobin cyanide (HiCN) technique, red blood cell count (RBC, direct method), serum iron (microparticle chemiluminescent immunoassa y and related kit) and concentration of serum transferrin receptor (STFR, ELISA method and related kit). ② Recovery test: Other 10 rats in control group were regarded as normal control group, and they were fed with routine feed and drank freely. The rest 30 rats of half genders in model group were randomly di vided into 3 subgroups: model control group, FeSO4 group and chocolate & orange juice group with 10 in each group. Rats in model control group were perfused with distilled water everyday; rats in FeSO4 group were per fused with FeSO4, and rats in chocolate & orange juice group were per fused with chocolate carrier and orange juice. The iron volume in the last two groups was 6 mg/(kg·d). At 40 days after intervention, the experiment was stopped. Concentration of ferrohemoglobin, RBC, serum iron, concentration of STFR and activity of plasma-protein aconitase were measured with atom-trapping atomic-absorption spectrophotometry; meanwhile, biological utilization rate of chocolate carrier & orange juice was calculated.MAIN OUTCOME MEASURES: ① Contents of herrohemoglobin, RBC,serum iron and STFR before experiment and after modeling; ② contents of ferrohemoglobin, RBC, serum iron, STFR and activity of plasma-protein aconitase before recovery test and at 40 days after experiment; ③ Related biological utilization rate.RESULTS: All 60 rats were involved in the final analysis without any loss. ① Comparison of blood index after modelling: Content of ferrohemoglobin, RBC and content of serum iron were lower in model group than those in control group (P ＜ 0.01), but content of STFR was higher than that in control group (P ＜ 0.01). ② Comparison of blood index and activity of plasma-protein aconitase in liver before recovery test and at 40 days after experiment: At 40 days after intervention, concentration of ferrohemoglobin,RBC and content of serum iron were higher in FeSO4 group and chocolate & orange juice group than those in model control group (P＜ 0.01); however, content of STFR was lower than that in model control group (P ＜ 0.01).At 40 days after intervention, activity of plasma-protein aconitase in FeSO4 group and chocolate & orange juice group was higher than those before recovery test (P ＜ 0.01). ③ Related biological utilization rate of chocolate carrier plus orange juice: Biological utilization rate of FeSO4 was regarded as 100%, and biological utilization rate of chocolate carrier plus orange juice was increased remarkably (106.7%).CONCLUSION: Chocolate carrier plus orange juice can improve IDA function and wildly use on treating IDA because of its good absorption. It is characterized by well biological utilization rate and good taste; therefore,it is a hot topic for trophology and foods produce presently.

For a long time, limited by the factors such as laparoscopic technology, and limited medical resources , the residents accepting standardized training are lack of mastery of the technology . Meanwhile, it is the key to the training of personnel training and reserve in the field for residents to contact the laparoscope as soon as possible and carry out scientific and effective training. Therefore, based on the traditional method, we have developed a new type of laparoscopic teaching system for the standardized training residents and increased and integrated the LAP GAME R operations training system and the real-time multimedia teaching platform. The preliminary practice effect is good.

Objective To observe the effects of Ketangte 2 on experimental diabetes in mice.Methods Adrenalin and streptozocin(STZ) were used to induce hyperglycemia mice model and diabetic mice model respectively.The mice were divided into five groups randomly:model group,glibenclamide control group,high-,middium-,low-dosage Ketangte 2 groups.The administration lasted consecutive 15 days.The blank control group and model group were treated with the same volume of saline.After treatment,the levels of blood sugar were measured in hyperglycemia mice induced by adrenalin,and the fasting blood-glucose,2-hour blood-glucose,insulin levels in diabetic mice induced by STZ were determined.Meanwhile the histopathological changes of pancreas were observed under light microscope.Results For STZ-induced diabetic mice,the hypoglycemic effect of Ketangte 2 was obvious(P

Objective:To construct I-Ad/IgG2b Fc baculovirus expression vector and express I-Ad/IgG2b Fc dimer fusion protein in Sf9 insect cells.Methods:I-Ad α,I-Ad β and IgG2b Fc gene sequences were amplified from BALB/c mouse lymphocytes by RT-PCR.I-Ad α and I-Ad β were connected with the leucine zipper sequence Fos and Jun respectively by overlapping PCR to form I-Ad α-Fos and I-Ad β-Jun.I-Ad α-Fos and IgG2b Fc fragments were ligated by restriction sites Xba I to form I-Ad α-Fos-IgG2b Fc recombination sequence.I-Ad α-Fos-IgG2b Fc and I-Ad β-Jun fragments were inserted to PPH and PP10,which were the downstream of the promoters in the plasmid pFastBacTMDual,to form pFastBacTMDual+[I-Ad/IgG2b Fc] recombinant plasmids.The constructed vector was identified by PCR,restriction endonuclease and sequencing.The recombinant plasmids pFastBacTMDual+[I-Ad/IgG2b Fc] was transferred into the DH10Bac competent cell to form recombinant baculovirus Bacmid+[I-Ad/IgG2b Fc].The recombinant baculovirus was transfected into Sf9 insect cells by liposome transfection reagent.After infected with Sf9 insect cells,the supernatant was collected and concentrated by PEG20000 to obtain I-Ad/IgG2b Fc dimer fusion protein.The fusion protein was detected by double-antibody sandwich ELISA and Western blot.Results:PCR,restriction enzyme digestion and sequencing confirmed that the recombinant vector pFastBacTMDual+[I-Ad/IgG2b Fc] had the correct sequence.The double antibody sandwich ELISA and Western blot showed that recombinant bacmid could successfully infect Sf9 insect cells,and the expressed fusion protein had the correct conformation.Conclusion:The pFastBacTMDual+[I-Ad/IgG2b Fc] baculovirus expression vector was successfully constructed and expressed in Sf9 insect cells,laying a foundation for the study of I-Ad-restricted T cells.

Objective To explore the verification process for the analytic performance of the quantitative project of molecular di-agnosis.Methods Based onMedical laboratory accreditation criteria for quality and competence in the field of molecular diagnos-tics application note(CL-36)(2014)and the relevant documents published by Clinical and Laboratory Standards Institute (CLSI), the performance verification methodology of PCR detection for hepatitis b virus nucleic acid was achieved.for.Results The within-run precision of DNA detection for the hepatitis b virus was 0.109 and 0.105;and the between-run precisionwas 0.1 57 and 0.137. Compared with the reference laboratory,the regression equation was Y =0.947+0.343X ,and the linear correlation coefficient was 0.990.The linear range was 5.00-1.10 and thequantitative detection limit was 500 IU/mL.Hemolysis had no effect on the detec-tion of samples.Conclusion The laboratory with molecular diagnostic program should conduct analytic performance verification,and the appropriate method should be chosen to clear performance verification.Conclusion Clearing the performance indicators of de-tection projects has a very positive role in the clinical use of detection projects..