1.Study on the Antioxidative Effects of Suaeda heteoptera Kitag.
Chinese Journal of Marine Drugs 1994;0(04):-
Antioxidative effects of Suaeda heteoptera Kitag. extracts were measured using thiobarbituric acid method.The results showed that extracts obtained with water,methanol and hexane gave significant resistance to the lipid peroxidation of linolenic acid and hexane extract showed the highest antioxidative activities.Mechanism of this antioxidative effect was studied using chemiluminescence.The results showed that extracts can effectively scavenge O -? 2 but have no effects on OH ?.This illustrated that the antioxidative effects were achieved by scavenging O -? 2 radical.
2.Changes and significance of plasma neuropeptide Y in newborns with asphyxia
Dian HU ; Hang GU ; Zhijun JIN
Chinese Journal of Perinatal Medicine 1998;0(03):-
Objective To investigate the relationship between plasma neuropeptide Y(NPY) and asphyxia in newborns. Methods Thirty-seven patients with neonatal asphyxia (asphyxia group) and 33 healthy pregnant women(control group )in their third trimester were chosen. The concentrations of plasma NPY were measured by radioimmunoassay. Results The levels of NPY in umbilical artery plasma was (187.47? 46.63) ng/L in asphyxia group,and (115.33?29.42) ng/L in control group. There were significant difference between them ( P 0.05). Conclusions The neonatal asphyxia was associated with NPY. NPY might play an important role in the pathophysiological changes in neonatal asphyxia.
3.Study of the Relationship Between Air Pollution and Respiratory Diseases in Nanning
Wenshan HUANG ; Hengsheng HU ; Hang LIU
Journal of Environment and Health 1989;0(06):-
Objective To investigate the relationship between air pollution and respiratory diseases in Nanning. Methods To analyze the prominent pollutants in the last 12 years: total suspensible particulate (TSP), drop dust, sulphur dioxide (SO2) and nitrogen oxide (NOx) by using Shanghai Air Quality Index, and study the correlation between the above data and the constituent ratio of chronic odstructive lung disease(COPD) inpatients of Guanxi Zhuangzu Autonomous Region People's Hospital as well as the mortality of respiratory diseases in Nanning in the same period. Results The air pollution in Nanning was classified into coal pollution. The dominating pollutants were TSP, drop dust, SO2 and NOx. The epidemiology research showed that the mortality of respiratory diseases in industrial zone were 1.4-2.4 times of that in the city zoon, the city zone was less higher than suburb. During the most severe air pollution period in 1991-1995, the mortality of respiratory diseases was highest, and the inpatients of COPD in the hospital was relevant higher. Conclusion Air pollution is the primary environmental pathogeny and fatal factor for respiratory diseases.
4.Changes of Plasma Orphanin in Patients with Postpartum Depression
Dian HU ; Hang GU ; Xinru HONG
Chinese Mental Health Journal 1988;0(06):-
Objective:To study the changes of plasma orphanin (OFQ) level in post-partum depression (PPD). Methods:The level of plasma OFQ of 24 patients with post-partum depression (PPD group) and 25 healthy lying-in women (control group) were measured by radioimmunoassay.Results:The level of plasma OFQ in PPD group was 27.39?6.04 ng/L , and that of control group was 10.37?3.65 ng/L,the difference was statistically significant(P
5.A quantitative analysis of factors affecting BK viral DNA loads in early renal transplant recipients
Xiaoyong YANG ; Shihai LI ; Liang REN ; Wei WANG ; Hang YIN ; Hang LIU ; Xiaopeng HU ; Xiaodong ZHANG
Chinese Journal of Organ Transplantation 2012;33(8):477-480
ObjectiveTo investigate DNA loads and risk factors of BK virus infection in renal transplant recipients.MethodsWe developed a real-time PCR assay to quantitate BK virus loads in 80 patients receiving renal transplantation in our center,and correlation between the BK virus load and clinical course was analyzed.BK virus loads were measured in urine and plasma. Epidemiological features and risk factors of BK virus infection were analyzed.ResultsThe positive rate of BKV viruria and viremia in 80 renal recipients was 37.5% (30/80) and 8.75% (7/80),respectively.BKV loads were higher in renal allograft recipients whose age was more than 50 years old.BKV loads were observed in urine and plasma (compared with group whose age was less than 50 years,P=0.017 and 0.05,respectively).BKV DNA copies were higher in group Tac than that in group CSA (P<0.05),and the peak of BKV load in serum appeared at14th and10th month after transplantation,respectively,but the peak in urine was ahead of that in serum,appeared at 2nd and 8th month,respectively.ConclusionSerial measurement of BKV viral loads by quantitative PCR is a useful tool in monitoring the course of BK virus infection.The ages of recipients (>50 years) and using Tac + MPA can reactivate BK virus and then result in BKVAN in renal transplant recipients. Intensive BKV monitoring is necessary for these recipients.
6.Monitoring of Ciclosporin peak concentration in recipients during the stable stage following renal transplantation
Yong ZHANG ; Xiaodong ZHANG ; Yong WANG ; Xiaopeng HU ; Xiaobei LI ; Wei WANG ; Hang YIN ; Hang LIU
Chinese Journal of Tissue Engineering Research 2010;14(5):794-798
BACKGROUND: Documents recorded that the correlation between micro emulsion Ciclosporin peak concentration (C_2) and area under curve was best with maximum individual difference. According to C_2, dose of Ciclosporin can be adjusted indMdually to decrease acute rejection and Ciclosporin toxicity, which has widely used in perioperative stage of renal transplanted recipients. However, some transplantation center still used tough concentration (C_0) to adjust the dose of Ciclosporin in stable stage of renal transplanted recipients. OBJECTIVE: To analyze the efficacy and safety of changing from monitoring C_0 to C_2 in stable stage recipients following renal transplantation. METHODS: Totally 65 patients with renal transplantation were enrolled in this study, including 31 males and 34 females, aged 20-57 (39.4±15.3) years. Within 3 months prior to this study, all patients did not suffered from rejection, and their serum creatinine and urea nitrogen were stable (creatinine ≤180 μmol/L). They were in stable stage after renal transplantation. Their period of transplantation and function of allograft were recorded. Their C_0 and C_2 of Ciclosporin were assayed. According to the target C_2 value 500-600 μg/L, the patients were prospectively and randomly divided into 3 groups. In the high C_2 group (n=17), the dose of Ciclosporin was decreased. In the target C_2 group (n=23), the dose of Ciclosporin was remained. In the low C_2 group (n=25), the dose of Ciclosporin was increased. All of the patients were followed-up for 12 months. The grafts function and the complications of heart, lung and brain were compared. RESULTS AND CONCLUSION: According to the target concentration of Ciclosporin C_2, the dose of Ciclosporin in the high C_2 group was decreased by 575.0 mg. The Creatinine and urea nitrogen of 88% patients were stable, while blood pressure, blood fat and blood uric acid decreased in parts of patients. In the target C_2 group, the levels of creatinine, urea nitrogen, Co and C_2 of patients were stable, no complications of heart, lung and brain occurred. According to the target concentration of Ciclosporin C_2, the dose of Ciclosporin in low C_2 group was increased by 755.0 mg. The creatinine and urea nitrogen of 84% patients were stable. All of the patients were no complications of heart, lung and brain. It is safe and effective to adjust Ciclospori dose under C_2 monitoring according to the target peak concentration (500-600 μg/L) in most stable stage recipients following renal transplantation.
7.Signal transducer and activator of transcription 1 and matrix metalloproteinase 3's genetic expression and their clinical significance on urothelial tumor after renal transplantation
Xiaopeng HU ; Liang REN ; Hang YIN ; Xiaoyong YANG ; Wei WANG ; Hang LIU ; Xiaodong ZHANG
Chinese Journal of Urology 2010;31(10):687-690
Objective To investigate the signal transducer and activator of transcription 1(STAT1) and matrix metalloproteinase 3(MMP3)′s genetic expressions and their clinical significance on urothelial carcinoma after renal transplantation. Methods Fifty-one patients with urothelial carcinoma were recruited in this study. Sixteen of them who had renal transplant were in the experimental group and 35 of them without renal transplant were in the control group. All the cases had been proved postoperatively having transitional cell carcinoma by histopathological study. The human genome oligo arrays were used to analyze the gene expression spectrum of urothelial carcinoma after transplantation, aiming the STAT1 and MMP3's expression. Real time RT-PCR and immunohistochemical staining were used to compare the differences in the 2 groups. Results The experimental group showed that there were 35 genes up-regulated compared with the control group. Of them, 23had known gene function or partly known, and 12 had unknown gene function. There were 76 genes down-regulated. Of them, 46 had known gene function or partly known, and 30 had unknown gene function. After pathway analysis of the differentially expressed genes, there were 23 groups of pathways which had significant differences (P<0.05), referring to the aspects of immunosuppressive and tumor growth. The levels of STAT1 and MMP3 expressions had significant differences between the 2groups(P<0.05)as well. Conclusions The differential expression of urothelial tumor genes is obvious between patient who has had renal transplant and who has not. There are many aspects that are related to the tumor's growth like signaling pathways regulating proliferation, apoptosis of tumor cells, tumor angiogenesis and the tumor metastasis potential. STAT1 and MMP3 maybe become the targets of chemoprevention for post-transplantation urothelial carcinoma.
8.Post-transplant lymphoproliferative disorders following renal transplantation: 4 cases report and literatures review
Liang REN ; Xiaopeng HU ; Hang YIN ; Wei WANG ; Hang LIU ; Xiaodong ZHANG
Journal of Leukemia & Lymphoma 2013;22(7):444-447
Objective To study the clinicopathologic features of post-transplant lymphoproliferative disorders (PTLD) following renal transplantation.Methods Four cases of PTLD following renal transplantation were studied and relevant literatures were reviewed.Results All the 4 cases had received 3-drug-immunosuppression after transplantation.The duration between renal transplantation and diagnosis of PTLD was 5-112 months,averagely 34 months.The patients were suffered from infective monocytic hyperplasialike PTLD,plasmacytic hyperplasialike PTLD,polymorphic PTLD and monomorphic PTLD respectively in morphology and had no specific symptoms.All the patients received therapy with dosage reduction of immunosuppressants and some received rituximab or chemotherapy.The case of monomorphic PTLD died in a short time after diagnosis.Conclusion PTLD is a lymphoproliferative disease with distinctive morphologic and clinical characteristics.The main treatments include the dosage reduction of immunosuppressive agents,biotherapy and chemotherapy.The prognosis of monomorphic PTLD is poor.
9.Effect of cyclosporine on the genetic expression of matrix metalioproteinase-3 of bladder cancer in rats and its clinical significance
Liang REN ; Xiaopeng HU ; Hang YIN ; Wei WANG ; Hang LIU ; Xiaodong ZHANG
Cancer Research and Clinic 2013;(6):403-405
Objective To investigate the effect of cyclosporine on matrix metalloproteinase-3 (MMP-3)’ s genetic expression on bladder cancer in rats induced with BBN and its clinical significance.Methods Twenty SD rats were divide into experimental group or control group randomly.Ten samples of SD rats bladder cancer induced with BBN and cyclosporine simultaneously and 10 samples of SD rats bladder cancer induced with BBN only as control were used to observe the effect of cyclosporine on MMP-3’ s genetic expression.Real time RT-PCR and immunohistochemistry stain were used to analyze MMP-3 mRNA and protein levels of bladder cancer in rats respectively.Results The MMP-3 mRNA median expression were 7.6 (4.2-9.1) in experimental group and 4.7 (2.8-7.7) in control group.The MMP-3 protein expression were 1 case with (-),4 cases (+),5 cases (++) in experimental group and 3 cases (-),4 cases (+),3 cases (++) in control group.The differences of MMP-3 mRNA and protein levels of bladder cancer between experimental group and control group were both significant (P < 0.05).Conclusion Cyclosporine may stimulate the growth and development of bladder cancer through changing expression of some genes like MMP-3,and MMP-3 maybe become one of the targets of chemoprevention for post-transplantation bladder cancer.
10.Effect of cyclosporine on the genetic expression of signal transducer and activator of transcription 1 in bladder cancer of rats and its clinical significance
Liang REN ; Xiaopeng HU ; Hang YIN ; Wei WANG ; Hang LIU ; Xiaodong ZHANG
Cancer Research and Clinic 2013;25(8):520-522
Objective To investigate the effect of cyclosporine on signal transducer and activator of transcription 1 (STAT1) genetic expression on bladder cancer in rats induced by BBN and its clinical significance.Methods Twenty SD rats were divide into experimental group or control group randomly.Ten samples of SD rats bladder cancer induced with BBN and cyclosporine simultaneously as experimental group,and 10 samples of SD rats bladder cancer induced with BBN only as control.Real time RT-PCR and immunohistochemistry stain were used to detect STAT1 mRNA and protein level expressions of bladder cancer in rats respectively.Results The STAT1 mRNA median expression fold was 4.5 (2.1-6.6) in experimental group and 5.6 (3.4-8.5) in control group.The STAT1 protein expression were 5 cases with (-),3 cases (+),2 cases (++) in experimental group and 0 case (-),5 cascs (+),5 cases (++) in control group.The expression of STAT1 mRNA and protein level of bladder cancer between experimental group and control group were both significant different (P < 0.05).Conclusions Cyclosporine may stimulate the growth and development of bladder cancer through changing expression of some genes like STATI,and STAT1 maybe become one of the targets of chemoprevention for post-transplantation bladder cancer.