Objective:To observe the clinical effects of half puncture plus transcutaneous acupoint electric stimulation in the treatment of infantile facial paralysis. Methods:A total of 60 patients with infantile facial paralysis were divided into a treatment group and a control group by the random digital table, 30 cases in each group. The treatment group was treated with half puncture plus transcutaneous acupoint electric stimulation. The control group was treated with routine acupuncture. Before and after treatment, House-Brackmann (H-B) grading was used to evaluate the sick children respectively. The total effective rates were compared between the two groups after treatment. Results:After 6-course treatments, the total effective rate was 96.5% in the treatment group and 80.0% in the control group, there was a statistical difference between the two groups (P<0.05) in comparison of the total effective rate. After 6-course treatments, H-B scores were all elevated than those before treatment in the two groups (P<0.05); there was a statistical difference between the two groups (P<0.05). Conclusion:Half puncture plus transcutaneous acupoint electric stimulation is better than routine acupuncture in curative effect for infantile facial paralysis.

Objective To build the steady framework of the nasal tip by using caudal septal advancement technique to obtain the permanent nasorostral configuration.Methods From Oct 2009 to Apr 2011,the caudal septal advancement technique was used to reconstruct the septum to make the septal anterior angel and the caudal septal margin reach the anticipated height and length of the tip,then medial crus and the vault of the lower lateral cartilage were sutured to the caudal septum to form the steady supporting structure of the nasal tip.Results 3-16 months follow-up in 26 cases showed that 2 cases appeared the staircase deformity in the junction of the bone and the cartilage in the dorsum,but the nasorostral configuration was good.3 cases whose tip and columella were deviated before the operation appeared the deviation again after 3 months but the nasorostral configuration was better than before.The other 21 cases were good.Conclusions It is a available method of using caudal septal advancement technique to form the steady nasorostral configuration.

Nuclear factor kappa B (NF-κB) is an important nuclear transcription factor involved in a wide range of inflammatory mediators,adhesion molecules,chemokines and inflammatory-related enzymes in gene regulation,causing immune damage.Vascular cell adhesion molecule-1 (VCAM-1),an adhesion molecule,belongs to the immunoglobulin superfamily and can mediate adhesion of inflammatory cells and endothelial cells caused by inflammatory injury.VCAM-1 gene promoter region contains a series of many of the features of NF-κB site.NF-κB activation can increase the expression of VCAM-1 that causes the immune and inflammatory injury.Present study has found that immune inflammation is the basis of a variety of renal diseases.NF-κB,VCAM-1 in kidney disease and its research progress may be discussed in this article.

Objective To study the effects of two disinfection methods for simple respirators in order to select the better sterilization method for the disinfection of simple respirators.Methods One hundred and sixty simple respirators contaminated at the same degree were randomly divided into the manual group and the mechanical group equally .The former took the manual cleaning and disinfection and the latter the mechanical cleaning and disinfection for the disinfection and cleaning of simple respirators.The two groups were compared with regard to the qualified rate of disinfection at the outer and inner surface and the interface of the ball cavity after disinfection cleaning.Results The qualified rates of the mechanical cleaning and disinfection at various parts of the simple respirators were 100%.The qualified rate of manual cleaning and disinfection at the outer surface,the inner surface and the interface were 100%,91.25%and 93.75%,respectively.There was no significant difference in the disinfection effect at the outer surface and interface of simple respirators between the two groups(both P>0.05).But the two kinds of cleaning and disinfections at the inner surface of the respirators were significantly different as regards the qualified rate(P<0.05).Conclusion For the disinfection of simple respirator,the mechanical cleaning and disinfection is better than the manual cleaning and disinfection.

Objective To verify that the length of mice fibroblast telomere can be shortened by D-gal.Methods The fibroblasts were from the heart tissue and passaged for 3-5 generations.After the fibroblasts were mixed with(C_(3)TA_(2))_(3)PNA labeled with FITC,the variation of fibroblast telomere length was detected by Flow-Fish.Results The length of fibroblast telomere can be shortened by D-gal.Conclusion D-gal can lead to the fibroblast telomere shortening.It demonstrates that D-gal can cause cellular senescence,which establishes the foundation for the senescence model of heart fibroblast.

Objective:The aim is to study the apoptosis and c-fos expression occurred in the rat gut following traumatic brain injury,and to explore the potential role of mucosal apoptosis in the intestinal barrier dysfunction and the relation of c-fos expression with the mucosal apoptosis.Methods:Male Wistar rats were randomly divided into six groups(6 rats each group) including controls with sham operation and TBI groups at hours 3,12,24,and 72,and on day 7.Parietal brain contusion was adopted using weight-dropping method.Rat jejunum was taken at each corresponding time point.Apoptosis was detected by in situ immunohistochemical staining(TUNEL) and c-fos expression by immunohistochemistry.Results:Mucosal epithelium appeared to be the major apoptotic cells,and the number of apoptotic cells increased since 3 h after TBI,peaking at 72 h postinjury.The apoptotic cells initially located at the tip of villus and gradually spread to the whole villus.The c-fos expression of gut mucosa gradually increased after TBI,predominantly in epithelial cells,smooth muscle cells and lymphocytes and with the maximal expression appeared at 24 h,72 h and 7 d postinjury.Conclusion:It is highly suggested that intestinal mucosa apoptosis plays an important role in the pathogenesis of gut barrier damage,and c-fos expression might be highly responsible for the apoptosis,proliferation and renovation of the small intestinal mucosa.

This article introduces four cases of children moyamoya disease,female central precocious puberty,children restless legs syndrome and damsel retrograde menstruation,which were treated by Yang Shaoshan.Doctor Yang insists that the pediatric difficult diseases should be treated with the methods of nourishing yin,nourishing kidney and lower evil fire and clearing and nourishing the Spleen and Stomach,of which protecting yin essence is therapeutic core.

This article explored professor YANG Shao-shan's clinical experiences in treating senile diseases with ointment.He proposes that the TCM method is as following: First,invigorating kidney for protecting semen,pay attention to nourishing yin and decreasing yang.Second,invigorating spleen and stomach,pay attention to "vegetarian diet".Third,eliminating pathogenic factor,like phlegm and stasis.Forth,reinforcing the three treasures.

Objective:To introduce the experience on 8 cases living-related donor kidney transplantation(LD KT) in the past two years. Methods:8 patients underwent LDKT.The living donors(6 males and 2 females) received fully medical evaluation before operation. All cases were donated by blood relationship donors. CTA,ECT,HLA matching,PRA and CDC were done pre-operations. All t he donors underwent open nephrectomy,in which 5 cases donated right kidneys and 3 cases donated lef t kidneys. All the case underwent t he first renal transplantation. Triple-combined immuno suppressive protocols consisted of calcineurin inhibitors(CNI),MMF and steroid. Results:All donors were discharged in ten days postoperation. Af ter followed up for 2 months to 14 months,all the donors kept normal kidney function without impairment of life quality. All the recipients were discharged in 28 days with normal kidney function,and keep the normal renal f unction till to now. Conclusion: Accurate pre-operation assessment of the donor's safe and choose the donor kidney,good surgical technique are critical for the success. Optimal HLA matches and reasonable using of immunosuppressants can make the grafts survived long term. Living-rela ted donor kidney transplantation is a safe and effective reatment.

Objective:To explore the proper concentration of superparamagnetic iron oxide (SPIO) labeling bone marrow mesechymal stem cells and the biological influence of different labeling concentrations,and to evaluate the characteristics of magnetic resonance imaging by a 1.5 T MR scanner. Methods:BMSCs (5th)were incubated with different concentrations of SPIO particles. The location, distribution and the labeling ratio of SPIO particles in cells were observed. At an optimal concentration,samples of SPIO-labeled and unlabeled MSCs suspension in agar were imaged by MRI with T1WI,T2WI and T2*WI. and the signal intensity were measured and statistically analyzed.Results:Labeled SPIO particles were stained in cytoplasm. BMSCs were efficiently labeled (97% to 100%)in culture at 24 hours and particle concentrations(25 to 50?g Fe/ml medium). Compared with unlabeled cells,BMSCs loaded with SPIO had similar viabilitv and proliferation profiles at this proper range of labeling concentration. SPIO labeling caused a stronger low signal attenuation effect in T1WI、T2WI andT2*WI. Conclusion:BMSCs can be easily and efficiently labeled by SPIO without interference on the cell viability and proliferation,MRI visualization of SPIO-labeled MSCs is feasible,which provrdes basis for future in vivo studies.