Objective To observe the clinical efficacy of flexor-extensor alternate electroacupuncture with low-frequency stimulation in improving the wrist-hand function after high spinal cord injury.Method Forty patients with high spinal cord injury were randomized into a treatment group and a control group, 20 cases in each group. The treatment group was intervened by flexor-extensor alternate electroacupuncture with low-frequency stimulation, while the control group was by electroacupuncture. The Lindmark score, MAX and IEMG of sEMG were observed before and after intervention, and the clinical efficacies were compared between the two groups.Result The MAX, IEMG of sEMG and Lindmark scores were significantly changed after the treatment in both groups (P<0.05). The sEMG signal values and Lindmark score of the treatment group were significantly different from that of the control group after the treatment (P<0.05). The total effective rate was 95.0% in the treatment group versus 80.0% in the control group, and the difference was statistically significant (P<0.05).Conclusion Flexor-extensor alternate electroacupuncture with low-frequency stimulation is an effective method in improving the wrist-hand function after high spinal cord injury.

Ultrasound guided regional nerve blockade in children is a kind of safe,visualized and efficient regional nerve blockade which can be supervised,probe-controlled synchronously and share a brand future compared with traditional land-mark based technique and the intravenous opiates.The aim of this review is to give an overview of the research and application development of ultrasound guided nerve blockade in children undergoing groin surgery.

Objective To study the effects of two disinfection methods for simple respirators in order to select the better sterilization method for the disinfection of simple respirators.Methods One hundred and sixty simple respirators contaminated at the same degree were randomly divided into the manual group and the mechanical group equally .The former took the manual cleaning and disinfection and the latter the mechanical cleaning and disinfection for the disinfection and cleaning of simple respirators.The two groups were compared with regard to the qualified rate of disinfection at the outer and inner surface and the interface of the ball cavity after disinfection cleaning.Results The qualified rates of the mechanical cleaning and disinfection at various parts of the simple respirators were 100%.The qualified rate of manual cleaning and disinfection at the outer surface,the inner surface and the interface were 100%,91.25%and 93.75%,respectively.There was no significant difference in the disinfection effect at the outer surface and interface of simple respirators between the two groups(both P>0.05).But the two kinds of cleaning and disinfections at the inner surface of the respirators were significantly different as regards the qualified rate(P<0.05).Conclusion For the disinfection of simple respirator,the mechanical cleaning and disinfection is better than the manual cleaning and disinfection.

Objective To verify that the length of mice fibroblast telomere can be shortened by D-gal.Methods The fibroblasts were from the heart tissue and passaged for 3-5 generations.After the fibroblasts were mixed with(C_(3)TA_(2))_(3)PNA labeled with FITC,the variation of fibroblast telomere length was detected by Flow-Fish.Results The length of fibroblast telomere can be shortened by D-gal.Conclusion D-gal can lead to the fibroblast telomere shortening.It demonstrates that D-gal can cause cellular senescence,which establishes the foundation for the senescence model of heart fibroblast.

Objective:The aim is to study the apoptosis and c-fos expression occurred in the rat gut following traumatic brain injury,and to explore the potential role of mucosal apoptosis in the intestinal barrier dysfunction and the relation of c-fos expression with the mucosal apoptosis.Methods:Male Wistar rats were randomly divided into six groups(6 rats each group) including controls with sham operation and TBI groups at hours 3,12,24,and 72,and on day 7.Parietal brain contusion was adopted using weight-dropping method.Rat jejunum was taken at each corresponding time point.Apoptosis was detected by in situ immunohistochemical staining(TUNEL) and c-fos expression by immunohistochemistry.Results:Mucosal epithelium appeared to be the major apoptotic cells,and the number of apoptotic cells increased since 3 h after TBI,peaking at 72 h postinjury.The apoptotic cells initially located at the tip of villus and gradually spread to the whole villus.The c-fos expression of gut mucosa gradually increased after TBI,predominantly in epithelial cells,smooth muscle cells and lymphocytes and with the maximal expression appeared at 24 h,72 h and 7 d postinjury.Conclusion:It is highly suggested that intestinal mucosa apoptosis plays an important role in the pathogenesis of gut barrier damage,and c-fos expression might be highly responsible for the apoptosis,proliferation and renovation of the small intestinal mucosa.

This article introduces four cases of children moyamoya disease,female central precocious puberty,children restless legs syndrome and damsel retrograde menstruation,which were treated by Yang Shaoshan.Doctor Yang insists that the pediatric difficult diseases should be treated with the methods of nourishing yin,nourishing kidney and lower evil fire and clearing and nourishing the Spleen and Stomach,of which protecting yin essence is therapeutic core.

Objective To introduce the surgical technique for lower facial recontouring. Methods Lower face is composed of mandible, muscle and subcutaneous fat. Mandibular angle reduction and augmentation mentoplasty were performed to recontour the skeleton of the lower face and liposuction to reshape the soft tissue. The three above-mentioned procedures were used alone or combined to recontour the lower face depending on the anatomy of the patients. Results The procedures were undertaken in 731 cases for lower facial recontouring. All procedures were performed with local anesthesia. The mandibular angle reduction was taken through the intra and extraoral combined incision with micro saw. Silicone implant was used in augmentation mentoplasty with intraoral incision. Tumescent technique was adoped in facial liposuction. The results were satisfactory with no serious complications. Conclusion Mandibular angle reduction, augmentation mentoplasty and facial liposuction used alone or combined are simple, effective and safe in lower facial recontouring.

Objective: To evaluate the clinical effect of chyluria with different methods,flushing pelvis with argenti nitras and nephrolympholysis.Methods:53 cases of chyluria devied into two groups,recived the treament with these two different methods,then observed the rate of chyluria recurrence.Results:45.5%(10/22)cases which treated by flushing pelvis with argenti nitras were observed chyluria recurrence in a short time(average 1.3months),but only two cases(2/31,6.5%) were found chyluria recurrence of opposite side kidney in the nephrolympholysis group.Conclusions:nephrolympholysis is a safe and effective treament with chylria,better than flushing pelvis with argenti nitras.

Objective To study radiological_anatomy of greater omentum and to address the related clinical implications,and to provide anatomic data for the radiological diagnosis and surgical application of greater omentum. Methods The anatomic distribution and morphology of the greater omentum as well as the anatomic relationship of the organs in the vicinity were analyzed by using axial,coronal,sagittal and 3D images from 16_detector row spiral CT scans of 60 individuals.We assessed the advantages of these images as well as the clinical significance.The data were recorded and analyzed by using SPSS11.5 software. Results 1.Advantages of three sections: axial section,the display of the fatty tissue and the anatomic distribution;coronal section,the vasculature; sagittal section,the anatomic relationship of neighbour organs.2.Three_dimensional reformatted images showed the return of the gastroepiploic vein.3.Gastrocolic trunk was revealed in 67.2% of cases.The free_hanging portion seemed to have the capability of 'migration': it located in the subphrenic spaces in 20.7% of cases and distributed in the right lower quadrant predominantly in 17.2% of cases.The CT value of fatty density within greater omentum was(-104.97?10.78)Hu and no statistical significance was found in the difference between greater omentum and the subcutaneous tissue.Conclusion Gastroepiploic vessel is the landmark for the location of greater omentum.Axial,coronal and sagittal sections together with 3D reconstruction images could produce a full display of greater omentum and provide valuable data for the radiological diagnosis as well as surgery of reconstruction, pancreas and portal hypertension.

Objective:To analyze the cause and treatment of ureteral stenosis in the early stage after kidney transplantation.Methods:250 cases of the kidney transplantation since 1993 to 2007 were collected and analyzed, including 141 men and 109 women,240 cadaveric transplantations and 10 living dononr transplantations. Results:In 8 cases(3.2%) ureteral stenosis occurred in the early stage following the renal transplantation;including hematoma compression(2cases);stenosis of ureter- bladder stoma(2cases);ureter torsion(1case);transplantal kidney torsion(1case);stomal leak(1case) and hydropsia of spermatic cord(1case). 7 cases required another surgical operation to remove the obstruction and the renal function recovered in these cases. Conclusion:The ureteral stenosis in the early stage of kidney transplantation was mostly contributed to the surgical reasons such as mis-perfamance. The occurance rate of ureteral stenosis should be reduced if each one step of the kidney transplantation is carefully performed. The open surgical managemen is a direct and effective method to remove the obstruction.