Objective To investigate the antimicrobial‐resistant profile and genes carried by 80 staphylococcus aureus and analy‐size its antimicrobial‐resistant mechanism .Methods The bacteria identification and the antimicrobial susceptibility test were con‐ducted by VITEK‐2 compact automatic system .Methicillin resistant taphylococcus aureus (MRSA) were screened by disk diffusion method with cefoxitin .The polymerase chain reaction(PCR)was used to detect the antimicrobial‐resistant genes .Results The re‐sistance rates of 80 staphylococcus aureus to penicillin ,erythromycin and gentamicin were 91 .25% ,85 .0% and 71 .25% ,respective‐ly .All of the isolates were susceptible to linezolid ,vancomycin ,teicoplanin and tigecycline .Among the 80 isolates ,there were 22 MRSA ,accounting for 27 .5% ,and 13 were inducible antimicrobial‐resistant .57 (71 .25% ) carried antimicrobial‐resistant genes ,in‐cluding 6 genes .Of which ,21(26 .25% )carried ermB and aac(6′)/aph(2) simultaneously ,9(11 .25% )carried ermA ,mecA ,qacA simultaneously ,and all of the 9 were MRSA ,13(16 .25% ) only carried ermC .Conclusion The resistance rates of staphylococcus aureus were high to penicillin ,erythromycin and gentamicin .The various antimicrobial‐resistant genes were positive in staphylococ‐cus aureus .We should pay attention to the detection of the antimicrobial‐resistant gene and choose antibacterial drug rationally .