AIM: To investigate the effect of recombinant human interleukin-10 (rhIL-10) on IL-6 and TNF-? levels in serum and liver of mice exposed to lipopolysaccharide (LPS). METHODS: rhIL-10 was prepared by using genetic engineering technology. Mice were intraperitoneally with 500 ?g of LPS, and then were treated intravenously with various dosages of rhIL-10. The levels of IL-6 and TNF-? in hepatic tissue and serum were determined by ELISA at 12 h, 24 h, 48 h and 72 h post rhIL-10 treatment. RESULTS: rhIL-10 markedly inhibited the increase in IL-6 and TNF-? levels in hepatic tissue and serum at 12 h after rhIL-10 treatment in LPS-challenged mice, and the inhibition effect was significant at 24-48 h after rhIL-10 treatment (P

Objective To study the effect of procyanidins (PC) on mRNA and protein expression of par-4 and bcl-2 genes in PC12 cells induced by A?_ 25-35 . Methods Cell survival rate was evaluated by MTT assay and apoptosis was analyzed by Hoechst 33258-PI fluorescence staining. The expressions of mRNA and protein for par-4 and bcl-2 were tested by RT-PCR and Western blotting. Results Pretreatment with different concentrations of PC (5、10、20, and 30 mg/L) for 1 h increased the survival rate of PC12 cell in a dose-dependent manner. PC prevented the PC12 cells nuclei from shrinkage, condensation, and cleavage induced by A?_ 25-35 . PC decreased the expression of par-4 mRNA and protein, and increased the expression of bcl-2 mRNA and protein. Conclusion PC can protect PC12 cells from apoptosis induced by A?_ 25-35 in a dose dependent manner. The mechanism of protection is likely related to decreasing the par-4 gene expression and increasing the bcl-2 gene expression.

Objective To establish the pipeline and evaluate the feasibility of high-throughput sequencing method used in the detection of severe pneumonia pathogens.Methods Clinical control study was used.Bronchi alveolar lavage fluids (BALF) samples from 76 patients with severe pneumonia (treatment group) and 18 patients with tracheal malacia (control group) and without clinical detected pathogens were collected during March 2015 to December 2016 in Shenzhen Children′s Hospital.The pathogens in the samples were detected using clinical tests and high-throughput sequencing respectively.The results of high-throughput sequencing were confirmed by real-time quantitative PCR and the high-throughput sequencing method used in the detection of severe pneumonia pathogens was evaluated.The χ2 test was used to analyze the correlation of detection rate between the high-throughput sequencing group and the non high-throughput sequencing group.Results The pipeline and method of high-throughput sequencing used in the severe pneumonia pathogens detection was established.The pipeline included sample collection, DNA extraction, library construction, sequencing, and bioinformatic analysis.In 76 cases of patients with severe pneumonia, the results of high-throughput sequencing in 66 cases of bronchoalveolar lavage fluid specimens were positive.The sensitivity was 86.84%, which was significantly higher than the total sensitivity of traditional clinical detection methods including bacterial culture, immunofluorescence and quantitative PCR(68.42%,52/76),χ2=7.426,P<0.001.A total of 13 pathogens were detected in 66 positive samples of high-throughput sequencing, including Mycoplasma pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae and adenovirus, etc.Nine kinds of pathogens were detected in these samples through non-high-throughput sequencing.In the experimental group, the results obtained by clinical test and high-throughput (80.26%) were entirely consistent in 61 samples and not completely consistent in 15 samples (19.74%) specimens.These inconsistent results were mainly concentrated in the detection of adenovirus, Streptococcus pneumoniae and Haemophilus influenzae through high-throughput sequencing, whereas clinical cultures and immunofluorescence methods were not able to detect these pathogens.PCR validation showed that there was no significant difference between the results of high-throughput sequencing and the PCR tests (χ2=0.517,P=0.472), and the difference between the results of high-throughput sequencing and traditional clinical detection methods was statistically significant (χ2=11.671,P<0.001).Conclusion The method for the detection of severe pneumonia pathogens based on high-throughput sequencing is highly sensitive and can detect unknown pathogens, which is superior to those used in traditional clinical detection.

With the rapid development of information technologies and change of production method and life style,the visual health crisis has become a public health endangering the sustainable development of the economic society and national security,which shall be concerned and tackled by all levels of the society.Under the background of fully playing the role of traditional Chinese medicine (TCM) in preventive treatment,coping with the visual health crisis and satisfying the social needs for vision care,healthcare eye pad manufacturers with fairly scale have been emerged in China.However,there are no technical standards for healthcare eye pad products that are applicable for the whole country to standardize the production behaviors of the enterprises and protect the interests of the consumers.This research proposed the design and description for two technical standards of general requirements and inspection methods,as well as the vision on standards to be concerned and prepared by the eye healthcare industry in the future on the basis of analyses made for the contents and key indicators of the self-declared standards of the healthcare eye pad manufacturers.

Objective:To explore the clinical effect of de-stretching suture combined with electronic irradiation after resection of recurrent keloid.Methods:From May 2016 to August 2019, the patients with postoperative recurrent keloid were selected for the Department of Plastic Surgery, Yuncheng Central Hospital. The keloid lesions were resected, and the subcutaneous skin on both sides of the incision was extensively elevated to reduce the tension for direct suture. Local flap transfer was used when the direct suture is impossible due to too much tension. The PDS Ⅱ suture of 2-0 to 4-0 was used subcutaneously to reduce the suture tension with the "heart" suture technology, and the skin was intermittently sutured with the Prolene suture of 6-0 or 7-0 and 3M tensile tape was applied on the wound to reduce tension. Besides, electronic irradiation was performed within 6 hours and 1 week after the operation, with 8 Gy for each time, a total dose of 16 Gy. After the suture was removed, tension glue and elastic sleeve were used for external compression, and regular follow-up was conducted to observe the width and degree of scar hyperplasia of the patients. The therapeutic effect was evaluated according to the scar beauty evaluation and rating scale.Results:A total of 36 patients with postoperative recurrent of keloids were included in this group, including 28 males and 8 females, aged 17-68 years, with an average of 42.5 years old. The incisions healed in all patients after the operation, and no recurrence of keloids was found in the follow-up period of 18 to 36 months. The highest score of scar cosmetic evaluation and rating scale was 4, and the lowest was 0.Conclusions:Surgical resection combining with electronic irradiation is an effective method for the treatment of recurrent keloid.

Objective:To explore the clinical effect of de-stretching suture combined with electronic irradiation after resection of recurrent keloid.Methods:From May 2016 to August 2019, the patients with postoperative recurrent keloid were selected for the Department of Plastic Surgery, Yuncheng Central Hospital. The keloid lesions were resected, and the subcutaneous skin on both sides of the incision was extensively elevated to reduce the tension for direct suture. Local flap transfer was used when the direct suture is impossible due to too much tension. The PDS Ⅱ suture of 2-0 to 4-0 was used subcutaneously to reduce the suture tension with the "heart" suture technology, and the skin was intermittently sutured with the Prolene suture of 6-0 or 7-0 and 3M tensile tape was applied on the wound to reduce tension. Besides, electronic irradiation was performed within 6 hours and 1 week after the operation, with 8 Gy for each time, a total dose of 16 Gy. After the suture was removed, tension glue and elastic sleeve were used for external compression, and regular follow-up was conducted to observe the width and degree of scar hyperplasia of the patients. The therapeutic effect was evaluated according to the scar beauty evaluation and rating scale.Results:A total of 36 patients with postoperative recurrent of keloids were included in this group, including 28 males and 8 females, aged 17-68 years, with an average of 42.5 years old. The incisions healed in all patients after the operation, and no recurrence of keloids was found in the follow-up period of 18 to 36 months. The highest score of scar cosmetic evaluation and rating scale was 4, and the lowest was 0.Conclusions:Surgical resection combining with electronic irradiation is an effective method for the treatment of recurrent keloid.

Objective:To compare carotid endarterectomy (CEA) and carotid artery stenting (CAS) in perioperative, medium and long term prognosis of patients with carotid artery stenosis.Methods:A retrospective analysis was performed on 1 329 cases of carotid artery stenosis treated at Department of Vascular Surgery, Beijing Anzhen Hospital from Jan 2011 to Aug 2020, as all cases being divided into CAS group and CEA group.Results:There were significant differences in age ( t=0.098, P=0.023) and drinking habits ( χ2=8.055, P=0.005) between the two groups. There were more unstable plaques in CEA group ( χ2=4.392, P=0.038), and more bilateral lesions in CAS group ( χ2=9.673, P=0.038). In perioperative period, there were more mannitol use in CEA group ( χ2=78.614, P<0.001), more incision/puncture site complications ( χ2=5.158, P=0.035), lung infection ( χ2=6.355, P=0.013), cerebral hyperperfusion syndrome (CHS) ( χ2=5.158, P=0.035) and extracranial nerve injury ( χ2=23.760, P<0.001) in CEA group than in CAS group, and more acute renal failure in CAS group ( χ2=10.393, P=0.001). There was no significant difference in survival rate and ischemic stroke, myocardial infarction, cerebral hemorrhage and renal insufficiency between the two groups (all P>0.05). The mean survival time of CAS group was 53.195 months (95% CI: 52.040-54.350), and 54.492 months (95% CI: 53.790-55.195) in CEA group ( P=0.051). Conclusions:Patients in CEA group had more unstable plaque and a lower perioperative stroke rate. CEA group had higher risk of CHS,while CAS was with lower postoperative lung infection rate and less wound local complications. There was no significant difference in long-term survival between the two groups.

Chemical constituents of the Fufang Huangbai Ye( FFHB) were analyzed and identified by UPLC-ESI-LTQ-OrbitrapMS. The analysis was performed on an Waters HSS T3 reverse phase column( 2. 1 mm×100 mm,1. 8 μm). The mobile phase consisting of 0. 1% aqueous formic acid( A) and acetonitrile( B) was used with gradient elution,and the flow rate was 0. 3 mL·min~(-1).Based on the information of the accurate mass,the multistage fragment ions,the mass spectrometric data of the standard substance and the relative reference literature,the structure of the chemical constituents in FFHB were identified. Based on the identified compounds,network pharmacology study,including target prediction,functional enrichment,and molecular docking was applied to screen out the main active substances for treatment of diabetes foot and explore the potential mechanism. The results showed that a total of 138 compounds were identified,including 28 alkaloids,16 flavonoids,11 phenylethanoid glycosides,9 cycloolefins,11 cyclohexylethanol derivatives,28 phenolic acids and derivatives,3 lignans,4 terpenes,28 volatile oils and the others. Further,36 active substances for diabetes foot were screened out,and the functional enrichment showed the potential mechanism of FFHB were mainly seven functional items including inflammatory response,growth factor activity. This study combining the UPLC-LTQ-Orbitrap-MS technology and the network pharmacology provide a useful reference and basis for active compounds,quality control markers and the pharmacological mechanism of FFHB for diabetic foot treatment.
Chromatography, High Pressure Liquid ; Diabetic Foot ; drug therapy ; Drugs, Chinese Herbal ; pharmacology ; Humans ; Mass Spectrometry ; Molecular Docking Simulation ; Phytochemicals ; isolation & purification ; pharmacology