Objective: To establish a method for quality control of chitosan hemostatic Sponge. Methods: To study the indentification of chitosan , Lidocaine Hydrochloride ,Norfloxacin and inspect the suction water of sponge. Extracting Lidocaine Hydrochloride and Norfloxacin with acid and alkali then determining the content of them by spectrophotometic method. Results: The indetification experiment showed a good result . The suction water of sponge outstripped more than 20 times. The average content of Lidocaine Hydrochloride was 9.55mg/g ,Norfloxacin was 8.46mg/g. The average recovery of Lidocaine Hydrochloride and Norfloxacin was 97.9% and 96.5%. Conclusion: This method can be used to establish the quality control of chitosan hemostatic sponge.

Objective: The total triptolides in lactone leaves are generally extracted by decocting with chloroform,which is known to be a poisonous substance and pollutes the environment.The authors aimed to evaluate the use of macroporous resin in the extraction process.Methods: We measured the total triptolides by spectrophotometry and investigated the technology of extracting the total triptolides with macroporous resin,including determination of the concentrations of decoction and alcohol in the alcohol precipitation as well as the consumption of resin.Results: The total triptolides were enriched with the combination method of decoction by alcohol precipitation with macroporous resin.The ratio of concentration medicine to decoction was 1 ∶ 2,and that of the volume proportion of alcohol to decoction in the precipitation of alcohol was 2 ∶ 1.The appropriate amount of resin was 15%.Conclusion: Macroporous resin can be used to extract the total triptolides.

Objective: To investigate the extravasate sponging and hemostatic property of chitosan hemostatic sponge on the wound of otic vein of rabbit, and its healing effect on the scald of rabbit skin, for clinical application. Methods: Lacerating the otic vein of rabbit with bistoury and promptly pressing the wound with chitosan hemostatic sponge, gelatin sponge or gauze, respectively, the hemostatic effect was observed by uncovering each 30 seconds. Spreading chitosan hemostatic sponge, gelatin sponge or gauze, respectively, on the scalds of rabbit skin, the decrustation (pulling off scab) was observed within 10 days. Results: Comparing with the group of gelatin sponge, the group of chitosan hemostatic sponge showed significant differences in bleeding time and wound healing (P

12.08g herbs/kg.The overall effective rate for coronary heart disease was86.8%.CONCLUSION:Danzhisuxin capsule is appropriate in formulation and feasible in preparation technique.The method of quality control is reli?able.No toxic reactions were found and the clinical effect was satisfactory.

OBJECTIVETo explore the effects of Tripterygium extract (LLZ) on postburn hypertrophic scar - derived fibroblasts, so as to provide us an experimental support for the treatment of postburn hypertrophic scar.

METHODSHypertrophic scar - derived fibroblasts were harvested and cultured in vitro. A serial concentrations of LLZ (5 x 10(-3), 5 x 10(-4), 5 x 10(-5), 5 x 10(-6) g/L) were added to the culture media. The cellular morphology, proliferating activity and the cytotoxicity of the drug were observed after 24 hours of culture.

RESULTSThe fibroblast morphology could be altered by any concentration of LLZ solution. Furthermore, the cell count and the cellular proliferating activity were all decreased obviously by the treatment of LLZ.

CONCLUSIONThe cell morphology and proliferation activity of postburn scar-derived fibroblasts could all be inhibited by LLZ, and it was the result of its toxic effects.

Burns ; drug therapy ; pathology ; Cell Division ; drug effects ; Cell Size ; drug effects ; Cells, Cultured ; Child ; Child, Preschool ; Cicatrix, Hypertrophic ; drug therapy ; pathology ; Dose-Response Relationship, Drug ; Female ; Fibroblasts ; cytology ; drug effects ; Humans ; Male ; Phytotherapy ; Plant Extracts ; pharmacology ; Tripterygium