Objective To observe the effect of new moist dressings on pressure ulcers after spinal cord injury. Methods 58 spinal cord injured patients complicated with pressure ulcers were divided into observation group (n=29) and control group (n=29). The observation group accepted moist dressings, while the control group accepted routine dry dressings, TDP and ultraviolet irradiation. The incidence of im provement were recorded 2 weeks after treatment, and the time of healing were compared. Results 11 cases cured, 17 cases relieved in the observation group, and it was 5 and 18 cases in the control group, respectively (P<0.05). It spent (26.69±16.48) days to cure in the observa tion group, and (38.24±22.47) days in the control group (P<0.05). Conclusion The moist therapy may promote the cure of pressure ulcers af ter spinal cord injury, and shorten the time of cure.

Objective:To analyze the occurrence information and clinical manifestations of adverse reactions caused by quinolones antimicrobial drugs in Beijing Hospital and to provide the basis for clinical use.Method:In a retrospective study,128 cases of adverse reactions caused by quinolones antimicrobial drugs from 2003 to 2007 in Beijing Hospital were statistically analyzed in respect of drug categories,genders,ages,routes of administration,organs or systems, clinical manifestations,etc.Result:The drugs causing adverse drug reactions included seven categories.Levofloxacin showed the highest propotion,and intravenous drip was the main route of administration,and damages of skin were the most common manifestation.Conclusion:Adverse reactions monitoring of anti-infective agents should be strengthened to ensure the rational and safe patient medication.

To explore the expression and function of MG 7 Ag on gastric cancer cell line SGC7901 and its drug resistant subline,SGC7901/VCR, with different resistance index (0 3, 0 7, 1 0) The expression of MG 7 Ag on SGC7901 and SGC7901/VCR cells was determined by immunohistochemical method and flow cytometry (FCM) MTT assay was used to explore the effects of monoclonal antibody MG 7 on drug sensitivity of gastric cancer cells FCM was used to explore the effects of MG 7 on adriamicine accumulation of SGC7901/VCR cells SGC7901/VCR cells exhibit increasing positive staining of MG 7 than SGC7901, according to the increasing resistance index Sensitivity of vincristine of both SGC7901 and SGC7901/VCR cells was reduced after being treated with MG 7 And SGC7901/VCR cells showed decreased adriamicine accumulation when they were incubated with MG 7 Therefore, the conclusion is that MG 7 Ag may be a novel molecule associated with multidrug resistance of gastric cancer, and play an important role in maintaining the drug resistant phenotype of SGC7901/VCR cells.

Objective To study the effects of activation and inhibition agents of protein kinase C on the function and expression of P glycoprotein in drug resistant gastric cancer cells. Methods Immunohistochemical staining and FACS were used to determine the expression of P glycoprotein on drug resistant gastric cancer cells SGC7901/VCR and its parent cells SGC7901, which were treated or not treated by activation or inhibition agents of protein kinase C(PMA or H 7). Double labeled immunoflurescent and laser scan confocal microscopy were used to detect the coexpression of protein kinase C ? and P glycoprotein on drug resistant gastric cancer cells. Rohdamin 123 was used as fluorescent probe to observe the effects of PMA and H 7 on the function of P glycoprotein and drug accumulation in SGC7901/VCR. Results P glycoprotein had positive staining on SGC7901 cells and much stronger staining on SGC7901/VCR cells. Protein kinase C ? and P glycoprotein exhibited coexpression on SGC7901 cells and much higher coexpression level on SGC7901/VCR cells. When treated by PMA, SGC7901/VCR cells showed time dependent decreasing of drug accumulation, enhancing function and decreasing expression of P glycoprotein. When treated by H 7, SGC7901/VCR cells showed time dependent increasing of drug accumulation, weakening function and increasing expression of P glycoprotein.Conclusion Protein kinase C may play a role in multidrug resistance of gastric cancer cells via modulating the expression and function of P glycoprotein.

[Summary] Type 2 diabetic rat model accompanied by insulin resistance was established by high fat/high glucose diet and streptozotocin.Following metformin treatment for 4 weeks,realtime PCR and Western blot were used to detect the expressions of SIRT3 mRNA and protein in skeletal muscle tissue,respectively.The results showed that insulin sensitivity,superoxide dismutase (SOD),and glutathione (GSH) levels were significantly reduced in diabetic group compared with the control group (P＜0.05),while methane dicarboxylic aldehyde (MDA) level was increased (P＜0.05),along with the decreased expressions of SIRT3 mRNA and protein in skeletal muscles (P＜0.01).After metformin treatment,the insulin sensitivity,SOD,GSH,and SIRT3 mRNA and protein levels were significantly increased (P＜ 0.05 or P＜ 0.01),while MDA level was decreased (P ＜ 0.05),suggesting that metformin may ameliorate insulin resistance via upregulating SIRT3 expression in skeletal muscles of diabetic rats.

Objective To investigate the clinical features of osteonecrosis of the knee (ONK) in patients with systemic lupus erythematosus (SLE).Methods A retrospective chart review was conducted to identify patients of SLE with ONK.The clinical characteristics of these patients were analyzed.Results Between 2008 and 2013,eighty-one patients with SLE were analyzed in the 210th Hospital of People's Liberation Army.Eleven of these patients were diagnosed as ONK.All of these ONK patients were female.The age of onset ranged from 19-57 (36±13) years.The disease duration ranged from 4.5-31.0 (13±6) years.The local clinical symptom included knee swelling with pain in 5 cases,only pain with no swelling in 1 patient,no symptoms in 5 patients.All patients were with aseptic necrosis of the femoral head.For laboratory examination,7 patients had elevated triglyceride lipids in blood.Three of 7 patients accompanied with cholesterol level increased.Total cumulative dosage of oral corticosteroids was (66 824±27 328) mg.Conclusion ONK in patients of SLE is common and the majority of them have impaired renal function and elevated blood lipids,as well as long-term glucocortico-steroid therapy.

Adolescent ; Adult ; Cyclophosphamide ; adverse effects ; Female ; Femur Head Necrosis ; etiology ; Humans ; Immunosuppressive Agents ; adverse effects ; Male ; Middle Aged ; Paraquat ; poisoning ; Young Adult

OBJECTIVE:To investigate the satisfaction degree of outpatients on policy of separation of clinical pharmacy from 4 third grade hospitals in Shanghai,and provide reference for promoting medical reform policy. METHODS:Parts of outpatients in the 4 new suburb third grade hospitals were investigated by the questionnaire and interview in Shanghai city,and the data was sta-tistically analyzed by Excel software and SPSS 19.0 software. RESULTS:Totally 400 questionnaires were sent out and 368 were valid with effective recovery of 94.3%. The awareness rate of medical separation policy was 81.3% in the outpatients;55.5% of outpatients thought that medicine cost savings effect was not obvious;the overall satisfaction of patients was 91.6%,and the high-est of satisfaction was the service attitude and the lowest was medical expenses. CONCLUSIONS:Policy of separation of clinical pharmacy has reduced the medical expenses,guided the triage treatment of patients partly and improved the satisfaction of patients. It is suggested to shorten the waiting time by optimizing examination process,strengthen price regulation in drug,standardize pro-duction and distribution process,improve use rate of essential medicines and promote clinical rational drug use for further improve-ment of satisfaction.

It is to investigate the effect of two kinds of Houttuynia Cordata Injection on preventing and treating H1N1 influenza virus infection in mice. Pneumonia model was set up by intranasal infection of the normal and immunocompromised mice with influenza virus FM1 and PR8. The two injections were administered before and after the administration of virus, separately, and the lung index was observed. The results showed that the two preparations have obvious therapeutic effect on normal mice infected with influenza virus FM1 and PR8. And to FM1, the new injection's effect is better at small dosage. The results also showed that the two preparations have obvious prophylactic effect on immunodepressed mice infected with influenza virus FM1 and PR8. And to PR8, the old injection's effect is better at small dosage. Houttuynia Cordata Injection can improve the mice pneumonia caused by influenza virus H1N1 and decrease the lung index markedly. It has a remarkable preventive and therapeutic effect on H1N1 influenza virus in mice.