Phyllosphere and epiphytes have attracted significant interest of more and more researchers by their unique biological characteristics. Progress of research on phyllosphere and epiphytes was summarized in this paper, and the special emphasis was put on the characteristic of phyllosphere, microbial communities, invasion and the location of bacteria on leaf surfaces. Some ideas for further studies on phyllosphere microbes were also proposed.

Bacterial Infections ; Humans ; Respiratory Hypersensitivity ; microbiology

Asthma ; diagnosis ; Child ; Humans ; Perception

Adolescent ; Adult ; Cyclophosphamide ; adverse effects ; Female ; Femur Head Necrosis ; etiology ; Humans ; Immunosuppressive Agents ; adverse effects ; Male ; Middle Aged ; Paraquat ; poisoning ; Young Adult

Objective To study manganese superoxide dismutase (MnSOD) expression in colorectal cancer (CRC) cells and observe its relation to metastatic potential. To investigate the diagnostic performance of manganese-enhanced magnetic resonance imaging (MEMRI) for detecting metastatic potential of CRC. Methods High and low metastatic potential CRC cell lines SW620, HCT116, LoVo and SW480, DLD-1, HCT15, Caco-2, as well as normal colon mucosal cell CCD841 CoN were cultured. MnSOD expression level in cells was detected by western blot and the measurement was repeated for three times. HCT15, DLD-1, LoVo and SW620 cells were selected to perform in vitro MEMRI and subcutaneous xenografts were developed for subsequent in vivo MEMRI. MnCl2·4H2O solution was utilized as the contrast agent and T1 shortening was calculated. The differences of MnSOD expression level in cells, average T1 value shortening of cells and xenografts were separately compared by one-way ANOVA.Results The difference of MnSOD expression level in CRC cells were significant (P<0.05). All CRC cells have shown higher level of MnSOD expression than normal cell, except for Caco-2. The highest expression were shown in low metastatic potential cells (HCT15 and DLD-1). Whereas relatively low MnSOD expression was found both in high and low metastatic potential cells (LoVo, SW620, HCT116 and SW480). In vitro MEMRI demonstrated that the average T1 value shortening was greatest in high metastatic potential cells SW620 and LoVo [(289.33±0.57) and (268.45 ± 6.87) ms)], was significantly lower in low metastatic potential cells DLD-1 and HCT15, was lowest in CCD841 CoN [(65.12 ± 0.12)ms] (P<0.05). Twenty nine xenografts were successfully developed, high metastatic potential SW620 and LoVo tumors of 5 and 10 mm group showed greater T1 shortening, low metastatic potential DLD-1 and HCT15 tumors showed significantly less T1 shortening (P<0.05). However no significant difference was found in similar metastatic potential tumors (P>0.05). Conclusions MEMRI has the potential to noninvasively distinguish different metastatic potential CRC by revealing greater T 1 value shortening in more aggressive one. However the MnSOD expression in CRC cells is not corresponding to malignant potential.

Health Policy ; Humans ; Italy ; Occupational Health Services ; organization & administration ; Safety Management

Finland ; Health Policy ; Humans ; Occupational Health Services ; organization & administration ; Safety Management

Humans ; Infant, Newborn ; Male ; Marfan Syndrome ; complications ; Urinary Tract ; abnormalities

Objective To investigate the expression and clinical significance of Th17 and Tc17 cells in peripheral blood of lung cancer patients.Methods The percentages of Th17 cells and Tc17 cells in 60 lung cancer patients and 40 healthy controls were evaluated by flow cytometry analysis (FCM).Results The percentages of Th17 cells [(1.795±0.623) ％] and Te17 cells [(0.865±0.357) ％] in lung cancer group were significandy higher than those in controls [(1.405±0.256) ％,(0.640 ±0.204) ％],(t =28.944,P ＜ 0.001,t =14.051,P ＜ 0.001).Furthermore,there was a positive correlation between Th17 cells and Tc17 cells in the two groups (lung cancer group r =0.770,P ＜ 0.05,control group r =0.532,P ＜ 0.05).The percentages of Th17 cells and Tc17 cells were closely associated with clinical stage (F =4.882,P =0.011,F =3.633,P =0.033),but not connected with pathological types (P ＞ 0.05,P ＞ 0.05).Conclusion The overexpression of Th17 and Tc17 may be involved in the occurrence and development of lung cancer,which can be used as new indicators for immunologic function of lung cancer patients,and provide a reference in monitoring the disease.