Mammalian target of rapamycin (mTOR)is an atypical serine/threonine protein kinase,which is a key protein kinase regulating cell growth,proliferation,translation,metabolism and autophagy.It's first known beca- nuse inhibited by rapamycin.Research indicated that mTOR plays a key regulating role in physiological and patho- logical process of cardiovascular system.The present article mainly reviewed research progress of mTOR signal path- way effect in cardiovascular system.

Objective To screen for clopidogrel metabolism related gene CYP2C19 in patients with coronary artery disease in Wuhan.Methods 316 patients,from Jan to Dec 2014,after cardiology percutaneous coronary interventional therapy (PCI) for the treatment of coronary artery disease were selected as research object.Clopidogrel metabolism related CYP2C19 geno-types (* 1,* 2,* 3)were detected by the gene chip,and for different types of metabolism of patients according to CYP2C19 gene type:strong metabolize type (*1/*1),intermediate metabolizer types (*1/*2,*1/*3),poor metaboli-zer types (*2/*2,*3/*3,*2/*3).Results According to the CYP2C19 gene polymorphism of metabolic function type, strong metabolic type carrying CYP2C19*1 (*1/*1)accounted was 43.4%,intermediate metabolizers carrying CYP2C19*2 or *3 (* 1/* 2 and * 1/* 3)and poor metabolizers (* 2/* 2,* 2/* 3 and * 3/* 3)accounted was 42.4% and 14.2%,respectively.Different gender had no statistical significance in CYP2C19 genotype differences.Conclusion Clopi-dogrel metabolism functional of CYP2C19 gene in patients with interventional coronary heart disease in Wuhan area had more deletion gene.

Objective To analyze the safety and efficacy of early diet after laparoscopic resection of colorectal cancer.Methods A prospective study of 128 patients suffered laparoscopic surgery of colorectal cancer was done in our hospital.According to whether the adoption of early diet program,patients were divided into early oral group (EOF group,n=54)and traditional oral diet group (TOF group,n=74).Age,gender,preoperative nutritional status and other basic factors and postoperative ambulation time, postoperative hospital stay time,the number of cases the use of analgesics,complications of two groups were analyzed statistically. Results The first time of postoperative ambulation time and postoperative hospital stay time of EOF group were significantly re-duced compared TOF group [(1.2±0.8)d vs .(2.5±1.3)d]and [(3.2±1.4)d vs .(5.3±1.6)d](P <0.01).Conclusion Early diet after laparoscopic resection of colorectal cancer is good for patients fast recovery,and postoperative complications of two groups are similar.

Objective To research the function of hypoglycemic and antiobesity of L-Arabinose in New Zealand rabbit. Methods Thirty-two New Zealand white rabbits were randomly divided into 4 groups, 8 rabbits in each group. The control group was fed with high fat and high sucrose diet only. L-Arabinose group was fed with high fat and high sucrose diet along with L-Arabinose, the dosage were 0.308 5, 0.617, 0.925 5 g/kg respectively. After fed for two months, all groups were given L-Arabinose, and blood glucose was detected after orally given sucrose 0, 0.5, 2 h. After intervention of L-Arabinose for one month, the data of weight, food intake, stool quantity and the fat index were observed. Results L-Arabinose decreased significantly sucrose absorption, lower the fasting glucose level and the data of area-under-curve (AUC) significantly. Conclusion L-Arabinose proved to be highly effective in preventing the rise of circulating glucose and fat.

Rhamnolipid,an important biosurfactant,is reviewed with respect to chemical strocture,properties,physiological role and their fermentation production,especially focusing on the production with inexpensive raw materials,such as vegetable oils and residues from agro- industrial wastes.This can not only reduce the production costs,but also contribute to the reduction of environmental impact generated by the discard of residues,and the treatment costs.

Objective This investigation was to define the occupational security-related factors in community nurses and effective strategies to improve their service awareness and occupational protection ability.Methods A questionnaire was used for 1702 community nurses in Guangzhou to learn occupational security-related factors and occupational protection skills.Results Self-report results found that 21.3% (362/1702) nurses fully understood the occupational security-related factors,12.0% (204/1702) nurses knew the requirement for occupational security,and 12.3% (209/1702) nurse could provide occupational protection.Conclusions These results suggested that most community nurses showed poorer occupational protection awareness and ability.Thus,occupation safety management regulations,standardized performance procedure and emergency response plan should be put forward,and further community nurse training programme need to be initiated.

Objective To study the condition of benzodiazepines use in generalized anxiety disorder (GAD) patients and the relationship with the first benzodiazepine(BZD) prescription.Methods 70 outpatients with GAD were investigated retrospectively and divided into either research group( n = 54, M21, F33; age (18 ～73)year) or control group( n= 16,M7,F9; age( 18 ～53)year) based on whether the first prescription included BZD or not.Results The duration and dose of BZD used in research group ( separately (9.7 ± 4.4) months, ( 1.6 ± 0.7 ) mg/d) were significantly higher than those in control group ( separately(2.3 ± 2.4) months, (0.9 ± 0.9 ) mg/d) (P＜0.01).The long-term use of BZD was positively related to whether the first prescription include BZD or not,patients' insomnia on first visit,the duration of follow-up and the dose of BZD,which showed significantly difference (P＜ 0.05 ).The standardized rates of long-term use of BZD and dependence in research group were significantly higher than those in control group (P ＜ 0.01 ).Conclusion Prescribing BZD on the first visit is partly responsible for the long-term use of BZD and doctors should better be very careful of prescribing BZD on first visit.

Objective To evaluate the early changes of left ventricular (LV) diastolic function in patients with diabetes mellitus (DM) by two-dimensional speckle tracking echocardiography. Methods Thirty-six patients with DM and forty-five normal subjects (NOR) were studied. The peak early (E') diastole,peak late (A') diastole of longitudinal strain rate(SrL) in 18 myocardial segments and the average peak E' and A' of SrL on base segments, mid segments, apical segments were measured on apical long-axis views respectively. The global peak E'and A' of circumferential strain rate (SrC), radial strain rate (SrR) and rotation rate (RotR) at three levers were measured on short-axis views respectively. The ratio of E' and A', untwisting rate and untwisting half-time were calculated. At the same time, LV conventional ultrasound parameters were measured. Results Compared with NOR group in SrL,E' of 15/18 segments and E'/A' of 16/18 segments were decreased in DM group,A' of 14/18 segments were increased. The E' of global SrL and SrC, E'/A' of global SrL, SrC and SrR were decreased in DM group. The A' of global SrL, SrC and SrR, E' and E'/A' of global RotR were increased. There was no significant difference in LV conventional ultrasound parameters, untwisting rate and untwisting half-time between DM and NOR groups. Conclusions The decrease of SrL and SrC, increase of RotR and disappearance of myocardial regular movement in SrL and SrR are the early changes of diastolic dysfunction in patients with DM.

Objective To compare the clinical efficiency of two methods in the open surgery treatment of seconda-ry common bile duet stones: transeytie common bile duet exploration with eholedoehoscope (TCBDE),or traditional eholedoehotomy with T-tube drainage.Methods Retrospectively analysis the treatment of 113 eases with secondary bile duet stones (TCBDE group 58 cases ,T-tube group 45 cases) ; Compare the achievement ratio,operating time; complications,average hospital stay and cost between two groups.Results All procedure is successful,without any severe complications.There are no significant differences of operating time.The TCBDE group has sig-nificantly shorter hospital stay and lower cost.Conclusion TCBDE has significant advantages than the traditional choledochotomy,and should be the predominant method in the open surgery of secondary common bile duet stones.

Objective To explore the value of dynamic contrast-enhanced MRI (DCE-MRI)and diffusion tensor imaging(DTI)in grading of glioma.Methods 3.0T DCE-MRI and DTI scans were performed in 31 patients with glioma confirmed by pathology.Capacity volume transfer constant (Ktrans ),extravascular extracellular volume fraction (Ve ),exchange rate constant (Kep ),initial area under the gadolinium concentration-time curve (iAUC),and relative fractional anisotropy (rFA)were measured in the low grade glioma (LGG)and the high grade glioma (HGG).The correlation between parameters of DCE-MRI and rFA with microvessel denisity (MVD)and microvessel structure (MVS)were performed by Spearman rank correlation analysis.Results The MVD and MVS were positive correlation with the grading of glioma.Ktrans ,Kep ,Ve ,iAUC and rFA values of the LGG were (0.02±0.01)min-1 ,1.82 (0.18-8.54)min-1 ,0.05±0.03, 2.47±1.66 and 0.55±0.22,respectively.Ktrans ,Kep ,Ve ,iAUC and rFA values of the HGG were (0.1 1±0.02)min-1 ,1.31 (0.12-7.58)min-1 ,0.28±0.10,10.84 ±6.46 and 0.28 ±0.08,respectively.The differences of all parameters between the LGG and the HGG were statistically significant,except for Kep (P <0.05 ).Ktrans ,Ve and iAUC values were positive correlation with MVD and MVS (P <0.05),and rFA values were negative correlation with MVD and MVS (P <0.01).Conclusion The quantitative parameters of DCE-MRI and DTI have important values in grading of glioma and evaluating tumor angiogenesis and microvessel structure.