Hearing Tests ; Humans ; Infant, Newborn ; Neonatal Screening

Objective To observe the expression of glial fibrillary acidic protein ( GFAP), and the pathological and ultrastructnral changes of astrocytes in the CA1 subfield of the hippocampus following global cerebral ischemia and reperfusion, and to explore the neuroprotective mechanism of mild hypothermia. Methods Global cerebral ischemia was established in rats by a modified version of Pulsinelli's method. Ninety-six rats were divided into three groups including a sham-operated group, a normothermic ischemic reperfusion (IR) group and a hypothermic ischemic reperfusion (HIR) group. Each group had four subgroups which were sacrificed for 6, 12 or 24 hours, or 4 days after reperfusion (for each subgroup n = 8 ). Hematoxylin-eosin (HE) staining was used to observe morphological changes in neurons in the CA1 subfield of the hippocampus. TUNEL methods were used to detect apoptosis among those neurons. Immunohistochemical staining was used to detect the expression of GFAP in the CA1 subfield and the mechanism of astrocyte pathology. GFAP TUNEL double-labeled immunohistochemistry was used with both the shamoperated and experimental groups. Electron microscopy was also used to evaluate morphological changes in astrocytes 24 hours and 4 days after ischemia and reperfusion. Results Compared with the sham-operated group, expression of GFAP immunoreactive positive cells increased gradually in the CA1 subfield of the IR group rats. Compared with the IR group, expression of GFAP immunoreactive positive cells was significantly lower in the HIR group at all time points. Microscopic observation at the 4th day showed that some astrocytes in the CA1 subfield had died through oncosis. Conclusions Mild hypothermia can significantly decrease the expression of GFAP immunoreactive positive cells and the number of apoptotic neurons in the CA1 subfield of the hippocampus, minimize cell oedema and provide protection for neurons. Oncosis kills astrocytes following global cerebral ischemia and reperfusion.

BACKGROUND: Traditional Chinese medicine (TCM) has clear efficacy and fewer side effects, because of its own pharmacological action, as well as the trace elements in it.OBJECTIVE: To determine the contents of metal elements and their total flavone in Scutellaria baicalensis Georgi (SBG).DESIGN: Repeated measurement trial.SETTING: Jilin Medical College, Affiliated Hospital of Jilin Medical College.MATERIALS: SBG was bought on the market. Rutoside sample(F 20050930, Sinopharm Group Chemical Reagent Co., Ltd.).METHODS: The experiment was carried out in the Department of Trace Element in Chinese Herbal Medicine, Jilin Medical College from April to July in 2006. SBG samples were dried and powdered then digested with nitric acid-perchloric acid wet method. And 1.000 0 g was weighed accurately, 3 portions were used for each kind of sample, placed in 25 mL coni cal flask, 10 mL concentrated HNO3 was added, then sealed for overnight.10 mL concentrated HNO3 and 5 mL HClO4 were added respectively on the next day, and slowly heated on the electric hot plate; when only about 1 mL left, 20 mL concentrated HNO3 and 5 mL HC1O4 were added for continuous digestion till the solution was colorless and clearing and almost dried. It was diluted and dissolved with 30 g/L nitric acid, then transferred to 50 mL volumetric flask, evenly mixed for measurement. Atomic absorption spectrophotometer was used to detect macroelement Na, Ca, Mg, K and trace element Fe, Mn, Cu, Zn. Alcohol extraction method was applied to extract the total flavone of SBG while UV spectrophotometry for the content of total flavone.MAIN OUTCOME MEASURES: The contents of metal elements and their total flavone.RESULTS: The contents of metal elements and total flavone in SBG were detected as follows: K (1 098.516 8±2.731 9) μg/g; Ca (6 561.552 6±78.716 7) μg/g; Na (9 530.491 2±44.176 6) μg/g; Mg (6 555.442 8±36.860 3) μg/g; Fe (512.075 0±5.135 9) μg/g; Cu (2.905 0±0.134 2) μg/g;Zn (30.081 0±0.740 0) μg/g; Mn (12.409 2±0.247 7) μg/g; Total flavone(17.496 2±2.134 4) mg/g.CONCLUSION: Metal element and their total flavone are rich in SBG.

BACKGROUND: Epidemiological studies indicated that trace elements were closely correlated with the causes and pathogenesis of cerebrovascular disease, many trace elements play a beneficial role in maintaining the cerebrovascular and cardiovascular functions and structures, the trace elements needed in human body all exist in Chinese patent medicines, and different Chinese patent medicines contain different trace elements.OBJECTIVE: To make vague category of metal elements in 10 kinds of Chinese patent medicines commonly used to treat cerebrovascular diseases in clinic, and find the similarity of the contents of trace elementsand macroelements among the medicines, so as to provide evidence for researching the role of trace elements in the medicines and the correlation with the treatment of cerebrovascular disease.DESIGN: Ten kinds of Chinese patent medicines were powdered and accurately weighed, digested wit the HNO3-HClO4 wet method, the contents of metal elements were determined with atomic absorption spectrometry,mathematic matrix was applied in the vaguecategory of the data so as to find the similarities among the medicines.SETTINGS: Department of Chemistry, Jilin Medical College; Department of Neurology, Affiliated Hospital of Jilin Medical College.MATERIALS: The medicines were Naosetong tablet, Qiangli Tianma Duzhong Wan, Tongsemai tablet, Maitong capsule, Tongmai Ningxin Chongji, Fufang Yinxing Jiaowan, Dahuoluo Dan, Zhongfeng Huichun tablet, Xiaoshuan Tongluo capsule and Di 'ao Zhibituo, all of which could be bought on the market. Reagents: The standard reserving solution for Ca,Mg, Cu and Cr was the national criteria solution (NCS) with the concentration of 1.000 g/L (State Test Center of Ferrous Materials), that for Mn was the secondary NCS with the concentration of 500 mg/L (China General Station for Environment Monitoring). The concentration was 50 mg/L for Ca,Mg, Cu and Cr, 20 mg/L for Mn, HNO3 and HClO4 were both in excellent purity, and the water for the experiment was deionized water.METHODS: From March to June in 2001, 10 kinds of Chinese patent medicines commonly used to treat cerebrovascular diseases in clinic were selected in the Department of Chemistry, Jilin Medical College, dried and powdered, completely mixed, 1.000 0 g was weighed accurately with analytical balance, 3 portions were used for each kind of sample. The atomic absorption spectrometry was used to determine the contents of macroelements (Na, Ca, Mg, K) and trace elements (Fe, Ni, Mn, Cr, Cu, Zn), the ratios of Cu/Zn and Ca/Mg were calculated, the method of vague category was applied to study the correlations of the medicines.MAIN OUTCOME MEASURES: The differences of the contents of trace elements in different medicines were observed by determining their contents,and the similarities were found in the clustering analysis of the data.RESULTS: ① The contents of the trace elements and macroelements were rich in the 10 medicines, but they were different in different medicines.There were more macroelements in Naosetong tablet, Qiangli Tianma Duzhong Wan, Tongsemai tablet and Dahuoluo Dan; The trace elements were more in Tongsemai tablet and Maitong capsule. ② The contents of 10kinds of metal elements, Cu/Zn ratio and Ca/Mg ratio of each sample were taken as the indexes for standardized processing, and the method of maximal tree was applied for the clustering analysis. When r=0.55, five medicines had similar cluster, which were Naosetong tablet, Qiangli Tianma Duzhong Wan, Tongsemai tablet, Maitong capsule, Dahuoluo Dan and Xiaoshuan Tongluo capsule. ③ When r=0.85, three medicines had similar cluster, which were Qiangli Tianma Duzhong Wan, Tongsemai tablet and Maitong capsule. When r=0.95, the clustering analysis of trace elements of two medicines were extremely similar, which were Tongsemai tablet and Maitong capsule. Both Tongsemai tablet and Maitong capsule had the efficacies of increasing blood flow, ameliorate blood stream, and they had many similarities in treating cerebrovascular disease.CONCLUSION: Trace elements should have the similar actions in the process of drug treatment, the Chinese patent medicines with greater correlation coefficient in the clustering analysis have greater similarities in the efficacy on cerebrovascular disease, and those with smaller correlation coefficient also have fewer similarities in the efficacy.

Objective To investigate the value of thorough uterine curettage in microwave endometrial ablation(MEA).Methods Preoperative uterine curettage was conducted in 126 women with abnormal uterine bleeding(AUB)scheduled for MEA from June 2001 to June 2005.At the same period,another 12 patients with AUB underwent hysterectomy.And before the operation,uterine curettage and subsequent MEA were conducted.Then the uterine specimens were histopathologically and histochemically examined for evaluating the tissue damages.Results No intraoperative complications occurred in the 126 patients.Postoperatively,amenorrhea was observed in 78 patients(61.9%),normal menstruation was achieved in 46 patients(36.5%),and small amount of irregular vaginal bleeding was found in 2 patients(1.6%),the overall satisfaction rate being 98.4%(124/126).Postoperative complications included endometritis in 10 patients,hematometra in 2 patients,and dysmenorrhoea leading to hysterectomy in 2 patients.Follow-up observations were conducted for 6～28 months(22?4 months)in 126 patients,116 of whom were followed for more than 2 years.Follow-up findings showed satisfactory surgical effects.After MEA,the injury depth was 4.2～4.9 mm in the uterine fundus,4.1～5.7 mm in the anterior wall,4.7～6.6 mm in the posterior wall,and 4.0～4.7 mm in the uterine horn.Conclusions MEA with preoperative thorough uterine curettage can completely destruct the endometrium.The method offers good efficacy,high satisfactory rate,and low risk of tissue injury.

Objective: To study the possible mechanism of malnutrition in peritoneal dialysis patients. Methods: Multicenter prospective cohort study was designed . Forty-four peritoneal dialysis patients who survived two years after a cross-sectional study were included in the present study. We divided our patients into two groups according to the dietary protein intake(DPI) during follow-up period, DPI lower than 0.78 g/( kg?d) or DPI higher than 0.78 g/( kg?d). Patients' nutritional status was evaluated during the cross-sectional study and at the end of follow-up. 22 patients were divided into two groups according to the nutritional status improved or worsened in subjective global assessment (SGA) during the follow-up period. Besides nutritional status, patient's residual renal function, volume status and the incidence of cardiovascular event during the follow-up period were also evaluated. Results: Based on SGA , the incidence of malnutrition in low-DPI group was 60.9% in the cross-sectioned study and 45.5% after the follow-up. In high-DPI group, it decreased from 57.1% to 28.6%. The residual renal function declined significantly in worse nutritional status group, the incidences of volume overload and cardiovascular events during follow-up study were significantly higher in worse nutritional status group than that in improved nutritional status group. Conclusion: Malnutrition in peritoneal dialysis patients may not be simply due to lower protein intake. The changes in residual renal function, fluid overload and cardiovascular disease, accompanied with low dietary protein intake may be the possible cause of malnutrition in peritoneal dialysis patients.

A case of primary lymphosarcoma of the prostate gland proved by Silvermann needle biopsy was presented. Lymphosarcoma of the prostate is a rare condition, and only 23 cases have been reported in medical literature. In Culver's series, 74 per cent were sarcoma of fibrous origin (round cell, spindle cell sarcoma, myxosarcoma), 5 per cent rhabdomyosarcoma, 6 per cent lymphosarcoma, and 10 percent pleuriplastic or un-or de-differentiated sarcoma. A large tumor of the prostate, which is not hard and nodule occurs in a middle aged patient, should be considered of lymphosarcoma of the prostate. A case of lymphosarcoma of the prostate is added to the literature.
Biopsy, Needle ; Humans ; Lymphoma, Non-Hodgkin* ; Middle Aged ; Prostate* ; Rhabdomyosarcoma ; Sarcoma

A study was made of 234 patients with carcinoma of the cervix seen during the period from Aug.-1959 to Dec.-1963, who had come to the Departments of Obstetric and Gynecology of Yonsei Medical Hospital. Intravenous pyelogram, cystoscopy, and urine examination were done and N. P, N. was checked in addition to clinical examination before treatment. Intravenous pyelogram were classified into 1. Non-functioning 2. Hydronephrosis 3. Hydroureter as abnormalities Also cystoscopic finding were classified into 1. Elevation of bladder wall 2. Bullous edema 3. Neoplasm invasion. There were 51 patients who showed abnormality of intravenous pyelogram among the 234 patients with carcinoma of cervix, that was a unilateral abnormality in 38 cases and bilateral in 13 cases. Again, among 24(10.26%) non-functioning kidney in this series the left kidney was affected in 11 cases and right kidney in 6 cases. The 125 of 234 patients showed elevation of bladder wall in cystoscopic finding, and the posterior wall of bladder was elevated similarly in all stages. The urological study of patients with carcinoma of cervix are most essential works in addition to clinical examination, and it should be considered as a systemic disease for all physician especially gynecologist, radiologist, and urologist.
Cervix Uteri* ; Cystoscopy ; Edema ; Female ; Gynecology ; Humans ; Hydronephrosis ; Kidney ; Neoplasm Invasiveness ; Urinary Bladder ; Urinary Tract* ; Uterine Cervical Neoplasms

Objective To remind the attention to contraceptive in cesarean section group by analyzing the treatment of 104 cases cesarean scar pregnancy (CSP).Methods From April 2009 to November 2012,104 patients with CSP treated in Department of Obstetrics and Gynecology,Wuhan Union Hospital were studied retrospectively.The incidence,treatment,and average in hospital days and average cost of hospitalization were analyzed.Results From 2009 to 2012,the number of CSP was 10,19,29,and 46 cases annually which accounted for the proportion of the corresponding period of gynecological patients were 0.33％,0.63％,0.97％,and 1.53％.Bilateral uterine artery embolization plus curettage was the most effective treatment.The average transfusion rate was 25％.The average amount of in-hospital days were (14 ± 8) days,the average cost of hospitalization were (12954.97 ± 3888.89) yuan.Conclusions The CSP incidence was increasing sharply in China.Although the current treatment methods could greatly reduce complications of CSP,it did harm to the patients and their family.The key to reduce the occurrence of CSP included reducing the rate of cesarean section,and strengthening contraceptive education after cesarean section.

Objective To study the effect of human ?-globin matrix attachment region(MAR) on transgene expression in stably transfected CHO cells.Methods Expression vector was constructed,which contained the ?-globin MAR in both sides of Chloramphenicol acetyltransferase(CAT) reporter gene expression cassette in cis,then transfected into CHO cells.The CAT reporter gene expression was analyzed by ELISA method.Results The ?-globin MAR enhanced the CAT gene expression 5.5-fold in stably transformed CHO cells,while the transgene expression variation among individuals of transformants was decreased.Conclusion MAR increase transgene expression in stably transfected CHO cells.