1.Changes of p62 and XIAP expression after neoadjuvant chemotherapy in breast cancer and their significance
Ying HAN ; Zhengsheng WU ; Qiang WU
Chinese Journal of Clinical and Experimental Pathology 2017;33(5):481-484
Purpose To explore the change of autophagy after neoadjuvant chemotherapy (NCT) and its relationship with XIAP expression in breast cancer tissues.Method The expression of p62 and XIAP was detected by immunohistochemistry of EnVision two-step in 94 cases of breast carcinoma before and after NCT.The changes of p62 and XIAP expression after NCT were observed and their correlation was also analyzed.Results High expression rate of p62 and XIAP was 51.1% (48/94)and 84.0% (79/94) respectively in breast cancer tissues before NCT.The high expression rate of p62 was increased to 81.9% (77/94) and that of XIAP was reduced to 35.1% (33/94) in breast cancer tissues after NCT.The high expression rate of p62 was significantly increased while that of XIAP was significantly decreased after NCT (P both < 0.05).The sample rate of increased expression of p62 and decreased expression of XIAP was 73.4% and 75.5% after NCT respectively.Furthermore,the change of p62 expression was significantly negatively correlated with the change of XIAP (P < 0.01).Conclusion Suppression of XIAP and increased autophagic activity coexist in breast cancer after NCT,and XIAP may be involved in the negative regulation of enhanced autophagy.
2.Association between maternal serum soluble endoglin levels and preeclampsia or eclampsia in the third trimester
Lijun ZHANG ; Yuhuan HAN ; Ying WU
Chinese Journal of Perinatal Medicine 2009;12(6):401-403
Objective To explore the ralationship between maternal serum level of soluble endoglin (sEng) in advanced gestations and hypertensive disorders comlicating pregnaney(HDCP). Methods The serum levels of sEng were analyzed using enzyme-linked immunosorbent assay (ELISA). Blood samples were obtained from 62 pregnant women with HDCP at 35-39 weeks' gestation (20 gestational hypertension, 20 mild preeclampsia, 19 severe preeclampsia and 3 eclampsia), and 20 normal pregnant women at 37-39 weeks' gestation (control). Results The serum sEng levels in normal, gestational hypertension, mild preeclampsia, severe preeclampsia and eclampsia group were (6.24±0. 26) ng/ml; (6. 56±0. 29) ng/ml; (7.47±0. 31) ng/ml; (8. 71± 0. 37) ng/ml and (9.69±0. 28) ng/ml, respectively. The serum sEng levels in the preeclampsia and eclampsia group were significantly higher than those in the gestational hypertension and normal group (P<0. 01), that of the severe preeclampsia group was significantly higher than the mild preeclampsia (P<0. 01), and that of the eclampsia group was significantly higher than the preeclampsia group (P<0. 01). However, no difference was found between the gestational hypertension and normal group (P>0. 05). Conclusions The increased serum level of sEng may participate in the genesis of HDCP.
3.Clinical observation on effect of Xuebijing injection for treatment of patients with diabetic nephropathy
Ying XU ; Bin HAN ; Guobing CHEN ; Ling ZHONG ; Haiyan WU
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care 2014;(1):35-38
Objective To investigate the clinical therapeutic effect of Xuebijing injection for treatment of patients with diabetic nephropathy(DN)and its mechanism. Methods 60 DN patients were randomly divided into Xuebijing group and control group(each,30 cases). The patients in both groups received western conventional treatment,and the patients in Xuebijing group received additionally Xuebijing injection intra-venous injection once a day for 14 days. The fasting blood glucose(FBG),glycosylated hemoglobin(HbA1c),urinary albumin excretion rate(AER),blood urea nitrogen(BUN),serum creatinine(SCr),hematocrit(HCT),fibrinogen(Fg),whole blood viscosity,total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C)and interleukin -6(IL-6),tumor necrosis factor-α(TNF-α)and urine β2-microglobulin(β2-MG)levels before and after treatment were detected,and the curative effect was also observed in both groups. Results In the control group blood FBG,BUN,SCr,TC,IL-6 and TNF-αafter treatment were significantly decreased and HDL-C significantly increased compared with those before treatment(all P<0.05). Compared with those before treatment,in Xuebijing group after Xuebijing therapy,blood FBG,β2-MG,AER,BUN, SCr,TC,TG,HCT,blood viscosity,IL-6 and TNF-αwere significantly decreased,and HDL-C was obviously increased,but there were no significant differences in HbA1c,LDL-C and Fg before and after treatment. The above indexes were changed significantly in Xuebijing group compared with those in control group〔FBG(μg/L):6.98±1.14 vs. 9.73±1.62,β2-MG(μg/L):32.1±10.9 vs. 57.2±15.1,AER(μg/min):86.0±28.1 vs. 152.0±51.6,BUN (mmol/L):12.4±8.1 vs. 19.5±8.9,SCr(μmol/L):301.2±151.9 vs. 371.3±168.6,HCT:0.283±0.075 vs. 0.351±0.059,TC(mmol/L):3.4±1.8 vs. 4.1±1.5,TG(mmol/L):3.4±1.5 vs. 3.6±1.7,HDL-C(mmol/L):1.90±0.75 vs. 1.50±0.25, IL-6 (ng/L):8.96±2.07 vs. 12.75±2.47, TNF-α(pmol/L):17.85±4.75 vs. 20.87±4.90,P<0.05 or P<0.01〕. The total efficiency in Xuebijing group was significantly higher than that in control group(83.3%vs. 36.7%,P<0.01). Conclusion Xuebijing injection has significant protective effects on patients with DN,and the mechanism might be associated with increasing tissue perfusion and inhibiting excessive inflammatory cytokines release.
4.Acceptability research of the home fecal occult blood self-testing in the community for colorectal cancer screening
Junfang BAI ; Zitao WU ; Jianhui WANG ; Qiang SHEN ; Ying HAN
Chinese Journal of Health Management 2014;8(6):394-398
Objective To explore the feasibility of home-based fecal occult blood self-test (home FOBTself-test) in the community colorectal cancer (CRC) screening,thereby to improve the community's compliance with colorectal cancer screening.Method Community medical staff and residents in Dongcheng District was recruited by means of self-reported questionnaires about personal wishes for home FOBT self-test screening since August 2013 to January 2014.Finally,using x2 test and logistic regression analysis to analyze the results of the final questionnaire.Of the total of 6,147 copies of the questionnaire,5 943 copies were valid questionnaires (Community medical staff:resident=114:5 829).Results 88.6% of community medical staff and 69.1% of community residents expressed willingness to choose home self-test FOBT.Univariate analysis showed that wishes of community medical staff for FOBT screening were unrelated with gender (P=0.635),age (P=1.000),CRC high-risk or not (P=0.418),awareness of home self-test FOBT(P=0.693).The wishes of community residents for FOBT screening related with gender (P=0.012),age (P=0.000),CRC high-risk or not (P=0.000),awareness of home self-test FOBT (P=0.000).Colorectal cancer risk was the greatest impact on wishes for FOBT screening (OR 值 (95% CI) =8.594 (7.279-10.148)).Conclusions Home FOBT self-test was simple,fast,easy to use and widely accepted in CRC screening of community residents,therefore it may improve the community's compliance with colorectal cancer screening.Screening for CRC is strongly recommended.
5.Epidemiological survey of urinary Cadmium in Shengyang urban children
Lixin CAO ; Mei HAN ; Ying TANG ; Jinxiang SHEN ; Yubin WU
Chinese Journal of Applied Clinical Pediatrics 2014;29(23):1806-1809
Objective To evaluate the body burden of Cadmium(Cd) in normal children from Shenyang city,and to analyze the effect of Cd on kidney.Furthermore,to investigate the influences of living factors on the level of Cd in children,so as to make recommendations for children's health.Methods The subjects was composed of 1 170 healthy children recruited from 0 to 17 years old by cluster random sampling method.They were divided into three groups(0-5 years old group,6-11 years old group,12-17 years old group).Questionnaires were used to obtain essential information about age,gender,weight,socioeconomic status,medication,and so on.Second morning urine samples were collected to make routine analyses,urinary Cd(Cdob),urine microalbumin(MALB) and α1-microglobulin(α1-MG).Results 1.A total of 1 070 children including 544 males and 526 females were enrolled.2.The concentration of Cdob increased with age,in agreement with the level of urinary Cd corrected by urine specific gravity (Cdsg).However,the level of Cd in urine corrected by urinary creatinine(Cdcr) presented the opposite status with the age increasing.3.Cdcr and urine MALB adjusted by urinary creatinin(MALBcr) revealed a positive correlation(rs =0.45,P < 0.01).4.Preference values for Cdob and Cdsg,Cdcr respectively were:0-5 years old <0.56 μg/L,<0.83 μg/L and <2.17 μg/g Cr;6-11 years old <0.65 μg/L,<1.01 μg/L and <1.23 μg/g Cr;12-17 years old <0.74 μg/L,<1.15 μg/L and < 1.25 μg/g Cr.Conclusions There is an age-dependent cumulative increases in Cdob.Although renal damage was not found in this study,the uptake of Cd should be reduced as much as possible and it is necessary to strengthen the follow-up study of Cdob in body burden.
6.Influence of different ventilator circuit change frequency on ventilator-associated pneumonia
Ying TIAN ; Xueqin MA ; Yonggang LIU ; Guangying HAN ; Haiying WU
Chinese Critical Care Medicine 2016;28(9):817-821
Objective To explore the appropriate frequency of ventilator tube replacement by researching the influence of different ventilator circuit change frequency on ventilator-associated pneumonia (VAP).Methods A prospective randomized sampling study was conducted.The patients undergoing invasive mechanical ventilation over or equal to 3 days admitted to emergency intensive care unit (EICU) of the First Affiliated Hospital of Kunming Medical University from December 2012 to December 2015 were enrolled.The patients were divided into 3,7 and 10 days group according to the frequency of ventilator tube replacement.Bacteriology of ventilator tube and the incidence of VAP were compared among the groups.Results Ninety-eight patients were enrolled,mainly with the artificial airway of endotracheal intubation or tracheotomy,with 56 male and 42 female,aging 8 to 86 years with mean of (51.97 ± 17.56) years.There were no statistical differences in gender,age,Glasgow coma scale (GCS) score,cough function and application of glucocorticoid,enteral nutrition,atomization and sedative therapy among three groups,indicating that the risk factors among three groups were consistent.The bacteria detection rates of extension tube,breathe out tube,breathe in tube,and hydrops collection cup were 36.7%,36.7%,33.3%,and 33.3% respectively in 3 days group,and they were 73.0%,67.6%,62.2%,and 62.2% in 7 days group respectively,and were all 90.3% in 10 days group.It was showed that the bacteria detection rate in different pipe parts was almost the same with the same change frequency,and the rate was higher with the longer usage of ventilator tube (x2 values were 20.599,19.879,21.975,21.975,all P =0.000).The longer of the tube used time,the higher incidence of VAP.The incidence of VAP in 3,7,10 days groups were 26.7%,59.5% and 77.4%,respectively,but there was statistically significant difference among all groups (x2 =30.486,P < 0.001).Based on the value of 3 days group,the incidence of VAP in the 7 days group was 15.950 folds of 3 days group,and the incidence of VAP in the 10 days group was 18.333 folds of the 3 days group (both P < 0.001).Conclusion This study suggests that the longer of pipeline using time,the more serious degree of bacterial contamination of pipeline,the higher incidence of VAP.
7.Effect of angiotensin Ⅱ on the expression of MIF in macrophages
Yanning WANG ; Xuguang WANG ; Min ZHANG ; Ying HAN ; Fan WU
The Journal of Practical Medicine 2016;32(20):3372-3375
Objective To investigate whether angiotensin Ⅱ (AngⅡ) can regulate the expression of MIF in macrophages via the NF-κB pathway. Methods Western Blot, real time RT-PCR and ELISA were used in the present study. Results Western blot result showed that the expression of NF-κBp65 gradually increased with the increase of the concentration of AngⅡ. Results of real time RT-PCR and ELISA revealed that MIF mRNA expression and the content of MIF were significantly higher in AngⅡ group than those in the control group. PDTC could reverse the effect of AngⅡ on MIF mRNA expression and MIF secretion in macrophages. Conclusion AngⅡ can promote MIF mRNA expression and MIF secretion in macrophages via the NF-κB signaling pathway.
8.Differential diagnostic value of CT scan minimum attenuation values in adrenal adenomas and non-adenomas
Ying WU ; Miaoping ZHU ; Zhiiiang HAN ; Peiying WEI ; Zhikai LEI
Chinese Journal of Endocrine Surgery 2017;11(3):192-197
Objective To investigate the differential diagnostic value of CT scan minimum attenuation values (minAVsCT) in adrenal adenomas and non-adenomas.Methods CT scan data of 89 cases of clinical and pathologically confirmed adrenal adenomas were subjected to retrospective analysis and compared with data of 46 cases involving 50 non-adenomas (25 metastases,20 pheochromocytomas,3 lymphomas,and 2 cortical carcinomas).The distributions of mean attenuation values (meanAVs) ¥ 10 Hu and minAVs ¥0 Hu and CT histogram analysis with ≥ 10% negative pixels were observed in adrenal adenomas and non-adenomas,and the diagnostic sensitivity and specificity of these 3 methods for adenomas were calculated.Results The distributions of unenhanced meanAVs ¥ 10 Hu,minAVs ¥0 Hu,and CT histogram analysis with ≥ 10% negative pixels among cases of adenoma and non-adenoma were 62.9% (56/89) and 0% (0/50) (x2=52.687,P=0.000),84.3% (75/89) and 2% (1/50) (x2=83.917,P=0.000),and 77.5% (69/89) and 0% (0/50) (x2=83.917,P=0.000),respectively.The respective diagnostic sensitivities,specificities,false negative rate (FNR),false positive rate (FPR),positive pre dictive value (PPV),negative predictive value (NPV) and accuracy of these 3 methods for adenomas were 62.9%vs 84.3% vs 77.5%,100% vs 98.0% vs 100%,37.1% vs 15.7% vs 22.5%,0 vs 2% vs 0,100% vs 98.7% vs 100%,60.2% vs 77.8% vs 71.4%,and 76.3% vs 89.2% vs 85.6%.Conclusion Although the specificity and PPV of minAVs≤0Hu is slightly less than meanAVs≤10Hu and CT histogram analysis with ≥ 10% negative pixels,it exhibits the best sensitivity and accuracy with a simple operation,and is thus suitable for clinical application.
9.Hypertrophic cardiomyopathy with right aortic arch, right descending aorta, and Kommerell's diverticulum: a case report.
Xiao-han FAN ; Hai-ying WU ; Si-yong TENG
Chinese Journal of Cardiology 2009;37(8):755-756
Cardiomyopathies
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complications
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Diverticulum
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complications
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Hematoma
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complications
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Humans
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Male
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Middle Aged
10.Effect of metformin on expression of SIRT3 in skeletal muscle of rats with type 2 diabetes
Yuping SONG ; Chong HAN ; Jingli SHI ; Ying WU ; Zhimin LIU
Chinese Journal of Endocrinology and Metabolism 2013;(5):427-429
[Summary] Type 2 diabetic rat model accompanied by insulin resistance was established by high fat/high glucose diet and streptozotocin.Following metformin treatment for 4 weeks,realtime PCR and Western blot were used to detect the expressions of SIRT3 mRNA and protein in skeletal muscle tissue,respectively.The results showed that insulin sensitivity,superoxide dismutase (SOD),and glutathione (GSH) levels were significantly reduced in diabetic group compared with the control group (P<0.05),while methane dicarboxylic aldehyde (MDA) level was increased (P<0.05),along with the decreased expressions of SIRT3 mRNA and protein in skeletal muscles (P<0.01).After metformin treatment,the insulin sensitivity,SOD,GSH,and SIRT3 mRNA and protein levels were significantly increased (P< 0.05 or P< 0.01),while MDA level was decreased (P < 0.05),suggesting that metformin may ameliorate insulin resistance via upregulating SIRT3 expression in skeletal muscles of diabetic rats.