1.Relationship between circulating endothelial cells and tumor angiogenesis
Cancer Research and Clinic 2010;22(3):211-213
Circulating endothelial cells (CEC) are endothelial cells which are detected in the peripheral blood. There are very few CEC in healthy adults while the number is obviously increasing in patients with arthrosclerosis, diabetes mellitus, lupus erythematosus, et al. Nowadays, flow cytometry analysis and immunomagnetic isolation for CEC are employed successfully in clinic and scientific research. Several research findings have confirmed that there is intimate relation between CEC and tumorigenesis. Because of the important role in angiogenesis and tumor growth, CEC would be a perspective tumor marker in antiangiogenesis and would also predict the chemotherapy efficacy.
2.Pathological features of spindle cell tumor of the uterine and broad ligament in 145 cases
Shuqin XIAO ; Yaping WANG ; Lu HAN
Chinese Journal of Postgraduates of Medicine 2017;40(8):714-717
Objective To explore the types, clinical pathologic and immunohistochemical features of spindle cell tumors of the uterine and broad ligament. Methods Clinical pathological files of spindle cell tumors in the uterine and broad ligament from 2007.12 to 2015.12 were reviewed. Results The primary sites of the 145 cases was cervical in 11 cases (7.58%), uterine body in 119 cases (82.07%), the broad ligament in 12 cases (8.28%), and uterine or cervix and the abdominal wall in 3 case (2.07%). Histopathological types of the 145 cases was smooth muscle origin in 126 cases (86.90%), of which 120 cases had benign leiomyoma, 3 cases had uncertain malignant potential and 3 cases had leiomyosarcoma;endometrial stromal origin in 15 cases (10.34%), including endometrial stromal nodule (5 cases), endometrial stromal sarcoma (10 cases); endometrial stromal- variant (3 cases, 2.07%), including endometrial stromal nodule associated with smooth muscle differentiation (2 cases), endometrial stromal sarcoma associated with smooth muscle differentiation (1 case); gastrointestinal stromal tumor (1 case, 0.68%). Conclusions Origin of spindle cell tumors of the uterine and broad ligament is mainly smooth muscle, endometrial stromal origin, and endometrial stromal tumor variants, and gastrointestinal stromal tumors in rare cases. We need to combine the clinical site, pathological morphology and immunohistochemistry for diagnosis and differential diagnosis.
4.Conclusion of GAO Yu-chun's Experience in Acupuncture and Moxibustion in the ;Treatment of Hypertension
Yebo WANG ; Yixu HAN ; Pengpeng LI ; Yanjun WANG ; Xiao XING
Chinese Journal of Information on Traditional Chinese Medicine 2017;24(2):101-103
Based on the theory of the rise and fall of qi-blood in meridians, Professor GAO Yu-chun harmonizes the qi-blood and yin-yang for diagnosis and treatment of hypertersion from liver, spleen and kidney. With reference of the academic idea of“Preventive Acupuncture”, he investigated the qi and blood, treated diseases in the upper part by managing the lower part, pay attention to the syndrome differentiation of excess and deficiency, the different of acupoint property, and the treatment of acupuncture or moxibustion. Thus, through balancing yin and yang, the qi and blood are regulated to reach the therapeutic aim of“yin and yang in equilibrium”.
5.Roles of angiopoietin-2, Tie-2 and hypoxia-inducible factor 1alpha in angiogenesis of glioma.
Xiao-Han LI ; Xiao-Song WANG ; Cheng-Bo HAN ; Hua-Chuan ZHENG ; Xiang-Hong YANG
Chinese Journal of Pathology 2008;37(3):188-189
Adult
;
Aged
;
Angiopoietin-2
;
physiology
;
Female
;
Glioma
;
blood
;
physiopathology
;
Humans
;
Hypoxia
;
blood
;
metabolism
;
Male
;
Middle Aged
;
Neovascularization, Pathologic
;
physiopathology
6.Endoscopic ultrasound-guided fine-needle aspiration cytology diagnosis of solid pseudopapillary tumor of the pancreas: report of four cases and review of literature.
Qi-han YOU ; Xiao-ling WANG ; Wei DING ; Yan-li WANG ; Bo WANG ; Xiao-dong TENG
Chinese Journal of Pathology 2013;42(2):121-122
Adult
;
CD56 Antigen
;
metabolism
;
Carcinoma, Papillary
;
diagnosis
;
metabolism
;
pathology
;
surgery
;
Endoscopic Ultrasound-Guided Fine Needle Aspiration
;
methods
;
Female
;
Humans
;
Male
;
Neprilysin
;
metabolism
;
Pancreatic Neoplasms
;
diagnosis
;
metabolism
;
pathology
;
surgery
;
Receptors, Progesterone
;
metabolism
;
Vimentin
;
metabolism
;
Young Adult
;
beta Catenin
;
metabolism
7.Study on GATA-6 Gene on Inhibition of Endogenous Carbon Monoxide on Pulmonary Vascular Smooth Muscle Cells Proliferation
li, YU ; han-min, LIU ; xiao-jie, SUN ; juan, WANG ; xiao-qin, WANG
Journal of Applied Clinical Pediatrics 2006;0(17):-
Objective To investigate the effect of GATA-6 on endogenous carbon monoxide(CO) inhibited pulmonary vascular smooth muscle cells(PVSMCs) proliferation induced by platelet-derived growth factor(PDGF).Methods Tissue mass culture was done to get PVSMCs artery in SD rats.The PVSMCs were stimulated to proliferation by PDGF(20 ?g/L),and the 3 different concentrations of hemin[a substrate and inducer of heme oxygenase-1(HO-1)] add into the cultures to induce CO production.The PVSMC cell proli-feration was detected by 3-(4,5-dimethyl-2-thiazoly1)-2,5-diphenyl-2H-tetrazolium bromide(MTT) method,DNA synthesis was detected by thymidine incorporation,and GATA-6 mRNA expression was detected by reverse transcription polymerase chain reaction(RT-PCR).Results CO inhibited the PVSMCs proliferation induced by PDGF in a dose-dependent and time-dependent manner.Hemin with high concentration markedly inhibited the proliferation and DNA synthesis of PVSMCs.After 2 hours with PDGF,the expression of(GATA-6) mRNA markedly down-regulated,and began returned after 6 hours.However,CO could reversed this down-regulation.Conclusions CO can inhibit the proliferation of PVSMCs induced by PDGF.PDGF can result in the expression of GATA-6 mRNA down-regulated;the down-regulation is reversed by CO.This study suggested that CO maybe inhibit PVSMCs proliferation by regulating expression of GATA-6.
8.Evaluation of Sensitivities and Specificities of SARS-CoV Detection by Real-time Quantitative Reverse Transcription-PCR Assays
Lili XU ; Zhihong HU ; Hualin WANG ; Xiao HAN ; Fei DENG
Virologica Sinica 2009;24(3):187-193
The etiological agent of severe acute respiratory syndrome (SARS) was identified as a new coronavirus, termed SARS-CoV. Establishment of an efficient and sensitive diagnostic system of SARS-CoV genetic materials is crucial for SARS control. In this study, we quantified SARS-CoV mRNAs in both infected cell culture lysate and in supernatant by using Real-time quantitative revere transcription-PCR based on EvaGreen鈩?dye and Taqman-MGB probes. For extensive evaluation of sensitivities and specificities, 13 pairs of primers and 4 probes were designed based on different genes of SARS-CoV. Glyceraldehydes-3-phosphate dehydrogenase (GAPDH) was selected as the internal control gene. Results showed that S-gene-specific PCR was the most sensitive for detection, but because of its sequence variability in the different viral strains, primers and a probe based on the N gene were suitable substitutions. Meanwhile, we found the mRNA concentrations in cell culture lysates were much higher than in cell supernatant and facilited more sensitive detection of the SARS-CoV.
9.Bioinformatics analysis of OLFM1 epitope and preparation of polyclonal antibody
Xiaoying WANG ; Peige DU ; Chunfeng WU ; Xiao HAN
Chinese Journal of Immunology 2000;0(08):-
Objective:To analyze OLFM1 by bioinformatics and prepare its polyclonal antibody.Methods:According to the bioinformatics analysis and prediction of the possible high structure,hydrophobicity and antigenicity of OLFM1 and the principal of antibody project,a partial peptide of OLFM1 with 20-amino acid residues with was synthesized after homology search.The synthesized peptide was then used to immunize rabbit.The specificity and titer of polyclonal antibody against OLFM1 were identified via ELISA and Western blot.Results:Polyclonal antibody against OLFM1 was proved to recognize OLFM1 protein specifically,and its titers reached 1?32 000.Conclusion:By the bioinformatics analysis and prediction,the hydrophilicity and antigenicity of OLFM1 are analyzed.The polyclonal antibody against OLFM1 is successfully obtained.