Mammalian target of rapamycin (mTOR)is an atypical serine/threonine protein kinase,which is a key protein kinase regulating cell growth,proliferation,translation,metabolism and autophagy.It's first known beca- nuse inhibited by rapamycin.Research indicated that mTOR plays a key regulating role in physiological and patho- logical process of cardiovascular system.The present article mainly reviewed research progress of mTOR signal path- way effect in cardiovascular system.

Objective To research the function of hypoglycemic and antiobesity of L-Arabinose in New Zealand rabbit. Methods Thirty-two New Zealand white rabbits were randomly divided into 4 groups, 8 rabbits in each group. The control group was fed with high fat and high sucrose diet only. L-Arabinose group was fed with high fat and high sucrose diet along with L-Arabinose, the dosage were 0.308 5, 0.617, 0.925 5 g/kg respectively. After fed for two months, all groups were given L-Arabinose, and blood glucose was detected after orally given sucrose 0, 0.5, 2 h. After intervention of L-Arabinose for one month, the data of weight, food intake, stool quantity and the fat index were observed. Results L-Arabinose decreased significantly sucrose absorption, lower the fasting glucose level and the data of area-under-curve (AUC) significantly. Conclusion L-Arabinose proved to be highly effective in preventing the rise of circulating glucose and fat.

Objective To screen for clopidogrel metabolism related gene CYP2C19 in patients with coronary artery disease in Wuhan.Methods 316 patients,from Jan to Dec 2014,after cardiology percutaneous coronary interventional therapy (PCI) for the treatment of coronary artery disease were selected as research object.Clopidogrel metabolism related CYP2C19 geno-types (* 1,* 2,* 3)were detected by the gene chip,and for different types of metabolism of patients according to CYP2C19 gene type:strong metabolize type (*1/*1),intermediate metabolizer types (*1/*2,*1/*3),poor metaboli-zer types (*2/*2,*3/*3,*2/*3).Results According to the CYP2C19 gene polymorphism of metabolic function type, strong metabolic type carrying CYP2C19*1 (*1/*1)accounted was 43.4%,intermediate metabolizers carrying CYP2C19*2 or *3 (* 1/* 2 and * 1/* 3)and poor metabolizers (* 2/* 2,* 2/* 3 and * 3/* 3)accounted was 42.4% and 14.2%,respectively.Different gender had no statistical significance in CYP2C19 genotype differences.Conclusion Clopi-dogrel metabolism functional of CYP2C19 gene in patients with interventional coronary heart disease in Wuhan area had more deletion gene.

Objective To analyze the safety and efficacy of early diet after laparoscopic resection of colorectal cancer.Methods A prospective study of 128 patients suffered laparoscopic surgery of colorectal cancer was done in our hospital.According to whether the adoption of early diet program,patients were divided into early oral group (EOF group,n=54)and traditional oral diet group (TOF group,n=74).Age,gender,preoperative nutritional status and other basic factors and postoperative ambulation time, postoperative hospital stay time,the number of cases the use of analgesics,complications of two groups were analyzed statistically. Results The first time of postoperative ambulation time and postoperative hospital stay time of EOF group were significantly re-duced compared TOF group [(1.2±0.8)d vs .(2.5±1.3)d]and [(3.2±1.4)d vs .(5.3±1.6)d](P <0.01).Conclusion Early diet after laparoscopic resection of colorectal cancer is good for patients fast recovery,and postoperative complications of two groups are similar.

Rhamnolipid,an important biosurfactant,is reviewed with respect to chemical strocture,properties,physiological role and their fermentation production,especially focusing on the production with inexpensive raw materials,such as vegetable oils and residues from agro- industrial wastes.This can not only reduce the production costs,but also contribute to the reduction of environmental impact generated by the discard of residues,and the treatment costs.

Objective This investigation was to define the occupational security-related factors in community nurses and effective strategies to improve their service awareness and occupational protection ability.Methods A questionnaire was used for 1702 community nurses in Guangzhou to learn occupational security-related factors and occupational protection skills.Results Self-report results found that 21.3% (362/1702) nurses fully understood the occupational security-related factors,12.0% (204/1702) nurses knew the requirement for occupational security,and 12.3% (209/1702) nurse could provide occupational protection.Conclusions These results suggested that most community nurses showed poorer occupational protection awareness and ability.Thus,occupation safety management regulations,standardized performance procedure and emergency response plan should be put forward,and further community nurse training programme need to be initiated.

Aim To investigate the cytotoxic effect and mechanism of ampelopsin sodium ( AMP-Na ) on hu-man lung adenocarcinoma cell line GLC-82 alone or combined with carboplatin ( CBP ) . Methods The cytotoxic effect of human lung adenocarcinoma cell line GLC-82 was investigated by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide ( MTT ) colori-metric assay. Ultrastructure change of apoptotic GLC-82 cells was observed with transmission electron micro-scope. The changes of the cell apoptosis and the ex-pression of caspase-3 were analyzed with flow cytome-ter. Results Combined with AMP-Na, the IC50 of CBP decreased from (17. 10 ± 4. 78) mg·L-1 to <3. 12 mg·L-1(P<0. 01), showed that the combina-tion of AMP-Na and CBP had synergistic effect on GLC-82 cells ( CDI <1 ) . As with transmission elec-
tron microscope and flow cytometric analysis, the apop-tosis and necrosis ratios also increased in the combina-tion group. The necrosis ratios increased from (2. 56 ± 0. 41 )% to ( 71. 83 ± 5. 43 )% ( P<0. 01 ) . The ex-pression of caspase-3 was increased significantly after treated with AMP-Na or combined with CBP. Conclu-sions There is a synergistic cytotoxic effect on GLC-82 cells treated with AMP-Na combined with CBP. Ap-optotic cells and necrotic cells are found in GLC-82 cells treated with AMP-Na alone or combined with CBP. One of the mechanisms to induce apoptosis is probably that activation of caspase-3 mediates signal transduction pathway in cells.

Objective To evaluate the early changes of left ventricular (LV) diastolic function in patients with diabetes mellitus (DM) by two-dimensional speckle tracking echocardiography. Methods Thirty-six patients with DM and forty-five normal subjects (NOR) were studied. The peak early (E') diastole,peak late (A') diastole of longitudinal strain rate(SrL) in 18 myocardial segments and the average peak E' and A' of SrL on base segments, mid segments, apical segments were measured on apical long-axis views respectively. The global peak E'and A' of circumferential strain rate (SrC), radial strain rate (SrR) and rotation rate (RotR) at three levers were measured on short-axis views respectively. The ratio of E' and A', untwisting rate and untwisting half-time were calculated. At the same time, LV conventional ultrasound parameters were measured. Results Compared with NOR group in SrL,E' of 15/18 segments and E'/A' of 16/18 segments were decreased in DM group,A' of 14/18 segments were increased. The E' of global SrL and SrC, E'/A' of global SrL, SrC and SrR were decreased in DM group. The A' of global SrL, SrC and SrR, E' and E'/A' of global RotR were increased. There was no significant difference in LV conventional ultrasound parameters, untwisting rate and untwisting half-time between DM and NOR groups. Conclusions The decrease of SrL and SrC, increase of RotR and disappearance of myocardial regular movement in SrL and SrR are the early changes of diastolic dysfunction in patients with DM.

Objective To evaluate the reliability and validity of Diabetes Diet-Related Quality-of-Life Scale (DDR-QOL) in Chinese version,in order to provide an efficient instrument for assessing the quality of life of diabetes in diet therapy.Methods Translating and edit the DDR-QOL scale initially,275 eligible subjects were invited to the study.DDR-QOL scale and 36 item Short Form Health Survey (SF-36) were used to collect data which were conducted for evaluating the internal consistency,reproducibility,construct validity,convergent and discriminant validity of DDR-QOL.Results The total Cronbach's α was 0.86.The factor analysis suggested good construct validity of DDR-QOL.Significant correlations were found between DDR-QOL and SF-36.The quality of life of diabetes who is on the job or not can be identified by DDR-QOL.Conclusions The Chinese version of DDR-QOL is a valid and reliable tool to assess the quality of life of diabetes who are in diet therapy.

Objective To explore the risk factors of children with cerebral palsy during pregnancy and neonatal period in Xinxiang area.Methods A retrospective analysis of the relevant research data of cerebral palsy children in Xinxiang area was performed.The research objects were children with cerebral palsy born from May 1,2005 to April 30,2010.At the same time,3 healthy children were selected as the control group to analyze the related risk factors causing cerebral palsy in children.Results The risk factors of children with cerebral palsy in Xinxiang city were as follows : maternal nutritional status, vaginal bleeding during pregnancy, pregnancy-induced hypertension syndrome, and abnormal production history were associated with cerebral palsy (x2 =2.313,13.296,5.034,7.434, all P ＜ 0.05)during the perinatal period;related factors during neonatal period were premature birth,severe asphyxia, severe jaundice, and intracranial infection(x2 =4.637,29.50,4.633,5.107, all P ＜ 0.05).Multivariate Logistic regression analysis showed the history of severe asphyxia (OR =2.340,95 ％ CI: 1.250-4.440), severe jaundice (OR =4.110, 95％ CI:2.430-6.740) ,premature birth(OR =2.570,95％ CI: 1.410-4.770) ,pregnancy-induced hypertension syndrome (OR =2.350,95 ％ CI:I.020-5.440), vaginal bleeding during pregnancy (OR =73.600,95 ％ C1:3.060-17.750) and abnormal production history(OR =5.710,95％ CI: 1.250-26.310) were independent risk factors causing children with cerebral palsy.Conclusions The history of severe asphyxia, severe jaundice, premature birth, pregnancy-induced hypertension syndrome, vaginal bleeding during pregnancy and congenital dysplasia are independent risk factors of children with cerebral palsy in Xinxiang area.It should be strengthened to screen and standardize the management of high-risk newborn infants with cerebral palsy, and do well management for perinatal high-risk pregnant women management.Early prevention can help to reduce the incidences of cerebral palsy in local area.