BACKGROUND:Whether there are breast cancer stem cel microspheres in the breast cancer tissues and whether these microspheres have an impact on isolation and culture of breast cancer stem cel s after different cycles of neoadjuvant chemotherapy are stil unclear. OBJECTIVE:To explore the proliferation and differentiation of breast cancer stem cel s in breast cancer tissues after different cycles of neoadjuvant chemotherapy. METHODS:Breast cancer stem cel microspheres were isolated from the breast cancer tissues after different cycles of neoadjuvant chemotherapy to drawn a cel growth curve. Immunocytochemical method was used to detect ALDH1 expression. RESULTS AND CONCLUSION:Microspheres could be obtained from the specimens of neoadjuvant chemotherapy for two, three and four cycles rather than one cycle. At 3 days prior to culture, there was no difference in the number of cel s isolated after two-and three-cycle neoadjuvant chemotherapy;but after 3 days, the cel s from the three-cycle neoadjuvant chemotherapy proliferated faster than those from the two-cycle neoadjuvant chemotherapy;after 6 days, the cel growth curve of two-cycle neoadjuvant chemotherapy was in the plateau stage, and the proliferation of cel s from the three-cycle neoadjuvant chemotherapy showed a rapid increase trend. The positive expression of ALDH1 in the microspheres from the three-cycle neoadjuvant chemotherapy was higher than that from the two-cycle neoadjuvant chemotherapy. These findings indicate that breast cancer stem cel s from the specimens of two-and three-cycle neoadjuvant chemotherapy both have proliferation and differentiation potentials, and the specimens of three-cycle neoadjuvant chemotherapy or above are preferred.

Investigate the effect of prestorage white cell filtration on the cytokines content in blood. Methods: Ten units of whole blood was divided into two parts just after blood collection:one was used as control, the other was filtrated by leukoreduction filters. All units were stored at 4℃ for 6 weeks. Immediately after filtration and every week subsequently, samples for analses of IL-2, IL-8 were obtained. Results:The amounts of IL-2 and IL-8 increased during the storage period. In filtered units, the amounts of IL-8 did not increase during the study period, IL-2 still increased but was still lower than that of unfiltered units at the end of storage. Conclusion: Many cytokines, such as IL-2 and IL-8, were generated and accumulated in blood during blood storage. Leukocyte filtration may prevent this effect to some extend. Prestorage filtration may decrease FNHTRs by preventing the accumulation of cytokines in blood.

Objective To prolong the shelf life of RBCs stored at non-4℃, and improve the ability of blood supplying during wartime. Methods 12 bags of packed RBC were collected from 12 volunteers presenting blood, respectively, and each was averagely divided into 5 parts(groups): combination group, new solution group, ammonia group, control group and 4℃ group. The fist 4 groups were stored at room temperature, and were tested for ATP concentration daily. 4℃ group was stored at 4℃, and RBC ATP concentration was measured at the end of its shelf life, which served as a critical ATP concentration. When RBC ATP concentration of the fist 4 groups decreased to the level of critical ATP, the time of preservation was their shelf life. 6 bags of packed RBC were added the same solution as the combination group, and each was averagely divided into 4 parts(groups)after stored at room temperature for 12 days, which were washed for 0, 5,10 and 15min before centrifugation, respectively. The residual ammonia and the recovery ratio of RBC were measured after washing. Results The shelf life of combination group,new solution group,ammonia group and control group was 12,6,6 and 5 days, respectively. The residual ammonia could be effectively cleaned up after either washing 4 times with 5 min balance time or 3 times with 10 or 15 min balance time. The recovery ratio of RBC was 88.5% and 83.1% after washing 3 and 4 times, respectively. Conclusion The combination of new solution and ammonia was a good additive solution for RBC preservation at non-4℃, and the residual ammonia can be cleaned up using normal saline.

0.05). Conclusion Clopidogrel resistance may influence long-term prognosis in patients implanted with DES, and it may contribute to recurrence of ischemic events.

Objective To study the preservative indices of stored platelet and verify the effect of a new platelet incubator made in China. Methods 12 bags of fresh platelet rich plasma (PRP) were collected. Each bag was equally divided into 2.One bag was stored for 5 days in XHZ-IA platelet incubator which was made in China, and the other bag stored in FORMA-3606 platelet incubator made in USA. Some preservative indices of stored platelet e.g. aggregation rate, recovery rate after response to hypotonic shock, CD62p expression rate etc. were tested daily. Results There were no significant differences in these preservative indices between platelets stored in two platelet incubatorshas. Conclusion XHZ-IA platelet incubator has the same effect on PRP preservation as FORMA-3606 platelet incubator.

Objective To evaluate the possibility of interventional therapy for avascular necrosis of the femeral head, with different curing methods, way of introduction and cultural heritages. Methods Vasodilator, thrombolytics and promoting microcirculatory drug were directly injected into the feeding arteries of the avascular necrotic femoral heads, under the condition of applying the blood stoppage belt at the root of thigh with pressure before the drug injection into the femoral pronator and extension arteries. The treatment was repeated 45 d later. Urokinase 10 5 unite/d ?10 were administrated with venous infusion ipsilaterally on the 15th day after the beginning of the therapy. Imaging features and clinical symptoms were recorded and correlatively studied before and after the treament. Results Symptoms relief especially the pain reached 100% after the treatment with various degrees of bony repair and new bone formation. Furthermore, increase caliber of feeding small arteries for femoral head and multiplicity of microvasculature, shortening of opacification time were revealed by DSA. IV stage bony change showed mild or inconspicuous. Conclusions Interventional catheterization treatment for avascular femoral head necrosis, especially the patients of fore Ⅲ stage, is safe and effective.

Objective To retrospectively analyse the impact of imatinib mesylate(Gleevec)on HSCT outcome in patients with Ph(+)leukemias.Methods From June 2001 to June 2005,31 CML-BC/AP patients and 8 refractory Ph(+)ALL patients were treated with imatinib mesylate to induce remission,followed by allogeneic transplantation,and we evaluated its influence on engraftment,graft versus host disease(GVHD),overall survival(OS),disease free survival(DFS),relapse rate(RR)and transplant-related mortality(TRM).Results Eighteen of 39 patients achieved complete hematologic remission(CHR),9 patients bone marrow remission(BMR)and 4 patients partial response(PR),the overall response rate being 70.96%.After HSCT,all patients achieved complete allogeneic engraftment with a median of 14 and 13.5 days for neutrophil and platelet recovery,respectively.The cumulative incidence rates of Ⅱ~Ⅳ? and Ⅲ~Ⅳ? aGVHD were 61.53% and 15.38%.The probability of OS and DFS was(73.51?9.61)% and(61.28?12.37)%,when HSCT was performed in CR,compared with(36.36?14.50)% and(31.25?13.98)% in NR.The 3-year cumulative relapse rate(RR)was 20.41% vs 75.00%.Four cases died of transplant-related complications.Conclusion Pre-transplant imatinib mesylate seems effective and safe for refractory Ph(+)leukemias;it allows transplantation to be in a more favorable status,and leads to better outcome.

Objective To establish a simple and feasible method of preparing young erythrocytes and test some indexes of evaluating the mean cell age.Methods We collected 34 bags of whole blood from healthy blood donors,400ml/each bag,then eliminated WBCs by leukoreduction filter.After twice centrifuging and manual speration,we finally obtained young erythrocytes from the upper section of the red cell layer,the remains were old erythrocytes.These different age erythrocytes were separated by blood bags.Some indexes as pyruvate kinase,reticulocyte count,mean corpuscular volume(MCV),mean corpuscular hemoglobin concentraion(MCHC),erythrocytes deformation index EI were tested on the non-separation erythrocytes,young erythrocytes and old erythrocytes,simultaneouosly,the detecting hemoglobin recovery rate.Results There was significant difference of all indexes among the three kinds of erythrocyte products.The PK activity ratio of young erythrocytes' to non-preparating erythrocytes was 1 175?0 13,showed the mean cell age was about 40 days.The hemoglobin recovery rate was(48 37?3 82)%.Conclusions The method is effective for preparing young erythrocytes,PK,REI,MCV,MCHC and EI can be used to evaluate the young erythrocytes' mean cell age.

Objective To study the effect of oxigenated leukodepletion filter(OLF), in order to evaluate its potential value in battlefield trauma application.Methods 12 bags of whole blood were filtered by OLFs, while the other 12 by normal filters,some indexes including ,partial pressure of oxygen(PO 2),O 2 saturation of oxygen(SO 2),HbO 2 WBC,RBC counter and volume of RBC supernatant were measured before and after leukocyte filtration.Results PO 2,SO 2 and HbO 2 increased 57%,51% and 53% respectively after filtration by OLFs, the residual leukocyte count decreased to (3 37?0 54)?10 7 each bag,and the leukocyte clearance rate was (97 52?0 74)%.Conclusions OLF can improve the effect of blood transfusion and reduce some acute transfusion adverse reactions.Thereby,application of the OLF has binefits significantly to heal the wounded patients in battlefield trauma.

Objective To study the effect of leukoreduction transfusion on improving medical quality. Methods The cases in our hospital which had RBC transfusion records in the past 3 years were collected and divided into two groups: leukoreduction group(LR) and non-leukoreduction group(NLR). Through statistical analysis, the items related to leukoreduction were found out and the differences in these items between the two groups were compared. Results Operation complication, non-operation complication, infectious rate, transfusion reactions and mortality rate in LR group were significantly lower than those in NLR group. The transfusion of single components-RBC had less clinical side-effect than the transfusion of complex components including RBC. Conclusions Leukoreduction transfusion can effectively improve clinical therapy and promote the medical quality.