2.Breeding of High-yielding ?-galactosidase Strains from Protoplast of Aspergillus niger
Hai-Ying DU ; Hong-Wei YU ; Jun HAN ; Ning LI ; Ying-Min JIA ;
Microbiology 1992;0(06):-
The protoplasts of original Aspergillus niger strain Uco-3 were treated with the cooperation of UV and ?-ray to obtain the high-yielding strain producing the thermostable ?-galactosidase. Under the optimum conditions of formation and regeneration protoplasts were prepared. According to the interaction of positive mutation rate and radiation dose,the optimum condition was determined. The optimum dose of UV was 4 minutes and the optimum dose of ?-ray was 500 Gy. After mutagenetic treatment of protoplasts and selection from a lot of mutants,a mutant DL116 producing the thermostable ?-galactosidase was obtained. The ?-galactosidase activity of DL116 was increased from 16.27 U/mL to 44.37 U/mL,which was higher than that of strain Uco-3.
3.Preliminary study of semi-quantitative and quantitative dynamic contrast-enhanced MRI in evaluating the response to concurrent chemoradiotherapy in patients with non-small cell lung cancer.
Tao XIULI ; Ouyang HAN ; Wu NING ; Liu LI ; Ye FENG ; Song YING ; Wu PEIHUA ; Lyu LÜ
Chinese Journal of Oncology 2015;37(4):272-277
OBJECTIVETo investigate the capability of semi-quantitative and quantitative parameters of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) to predict the response to concurrent chemoradiotherapy( CCRT) in patients with non-small cell lung cancer (NSCLC).
METHODSA total of 24 patients with stage III or IIIB NSCLC, who underwent 3.0T DCE-MRI before CCRT, were enrolled in this study. Semi-quantitative and quantitative parameters were calculated by Funtool and Omnikinetics software. The relationship between these obtained parameters and tumor response was evaluated by Spearmen' s correlation analysis. The patients were classified into two groups according to the tumor regression rate after treatment, as response group (group A) and non-response group ( group B). Mann-Whitney U test was used to compare the parameters of responders and non-responders. The value of the parameters on predicting response was calculated by receiver operating characteristic curve (ROC).
RESULTSThe tumor regression rate after treatment was negatively correlated with time to peak (TTP) and the extravascular-extracellular volume fraction (Ve), and was positively correlated with signal enhancement ratio (SERmax) and volume transfer constant (Ktrans) (P < 0.05 for all). Statistical significant differences were found between group A and group B both in semi-quantitative and quantitative parameters (P < 0.05). Group A had a lower TTP value [(34.66 ± 16.37) s vs. (44.09 ± 17.41) s] and Ve value [(0.19 ± 0.03) vs. (0.25 ± 0.05)] than group B, whereas group A had a higher SERmax [(166.50 ± 44.95)% vs. (113.57 ± 46.62)%] and Ktrans [(0.41 ± 0.17) min(-1) vs. (0.28 ± 0.12) min(-1)] than group B (P < 0.05 for all). The ROC analysis indicated that when setting the threshold of Ve on ≤ 0.21 for predicting response, the specificity, sensitivity and accuracy were 85.7%, 80.0% and 83.3%, respectively, with an area under curve of 0.875 (P < 0.001).
CONCLUSIONSBoth the semi-quantitative and quantitative DCE-MRI parameters are helpful for predicting the response after CCRT of NSCLC. Quantitative parameters seem to be more meaningful than semi-quantitative parameters.
Carcinoma, Non-Small-Cell Lung ; pathology ; therapy ; Chemoradiotherapy ; methods ; Contrast Media ; Humans ; Lung Neoplasms ; pathology ; therapy ; Magnetic Resonance Imaging ; methods ; ROC Curve ; Remission Induction ; Sensitivity and Specificity ; Time Factors
4.The effect of A375 cells corylin melanin synthesis
Yuanyuan PEI ; Guoliang LIU ; Shu ZENG ; Ying HAN ; Lei WANG ; Yan QU ; Jinghua CHEN ; Ning ZHANG
International Journal of Traditional Chinese Medicine 2017;39(5):430-434
Objective To study the effect of Corylin on A375 cells melanin synthesis,and explore its mechanism.Methods The cells were randomly divided into the control group, the estradiol group, and corylin group including 10-3μmol/L, 10-2μmol/L, 10-1μmol/L, 1μmol/L, 10μmol/L, 100μmol/L. Estradiol estradiol intervention group were given 10-3 mol/L. Corylin group (10-3μmol/L, 10-2μmol/L, 10-1μmol/L, 1μmol/L, 10μmol/L,100μmol/L) were given 10-3μmol/L, 10-2μmol/L, 10-1μmol/L, 1μmol/L, 10μmol/L, 100μmol/L corylin intervention. The activity of proliferation were detected by MTT, NaOH method, dopa oxidation , both melanin content and tyrosinase activity (tyrosinase, TYR). TYR, yrosinase related protein (tyrosinase related protein, TRP)-1 and TRP-2 expression levels of mRNA were determined by RT-PCR.Results Compared with the control group, 10, 100μmol/L of Corylin group cell proliferation rate significantly decreased (P<0.01). The 1μmol/L, 10-1μmol/L, 10-2μmol/L of Corylin group cell melanin content, TYR significantly decreased (P<0.01 or P<0.05). The 1μmol/L corylin group TYR (0.303 ± 0.003vs. 0.628 ± 0.012), TRP-1 (0.313 ± 0.008 vs. 0.677 ± 0.022), TRP-2 (0.456 ± 0.028vs. 0.687 ± 0.020) mRNA expression level significantly decrease (P<0.01).Conclusions The results showed that Corylin could inhibit melanin synthesis, which worked probably through inhibiting the activity of TYR and cutting the mRNA expressions of TYR,TRP-1/2.
5.Evaluate the effects of fluid loading on hemodynamics and cerebral perfusion under different levels of intra-abdominal pressure
Hui QI ; Qin GU ; Ning LIU ; Ying XU ; Yunhong HAN ; Beiyuan ZHANG
Chinese Journal of Emergency Medicine 2017;26(5):528-532
Objective To study the effects of fluid loading during fluid resuscitation on hemodynamics and cerebral perfusion(CPP)under different levels of intra-abdominal pressure(IAP).Methods Forty swine were employed in the study.Hypovolemia was made by blood withdrawal of 30% of estimated blood volume from each animal through the carotid artery line.All swine were randomized(random number) into four groups, namely IAPL0, IAPL15, IAPL25 and IAPL35.Then N2 gas was used to inflate in the abdomen slowly for elevating the IAP to 0,15, 25 and 35 mmHg.Fluid loading were performed with 500 mL hydroxyethyl starch within 30 minutes.Hemodynamic variables were evaluated by PiCCO.Heart rate (HR),mean arterial pressure(MAP), central venous pressure(CVP), cardiac output(CO), global end-diastolic volume index(GEDVI) and stroke volume index(SVI)were measured 30 minutes before fluid loading and 30 minutes after fluid loading.After placement of intracranial pressure optical fiber probe in the ventricle connected to intracranial pressure monitor for continuous monitoring of ICP changes, cerebral perfusion pressure (CPP) could be calculated.Results (1)HR decrease, MAP increased, SVI, CI and GEDVI significantly increased after fluid loading in all four groups (IAPL0,IAPL15,IAPL25 and IAPL35)(P<0.05),and there were not significant changes in CVP and EVLWI(P>0.05).SVRI decreased significantly in group IAPL15,IAPL25 and IAPL35 respectively(P<0.05).(2) ICP increased significantly in group IAPL15, IAPL25 and IAPL35(P<0.05), and CPP increased significantly in group IAPL25 and IAPL35(P<0.05) while CPP only slightly increased in group IAPL15(P>0.05).Conclusion When combined with intra-abdominal hypertension,fluid loading could improve the circulation of swine due to increase in CO and improve CPP.
6.Application of clustering analysis of data in iodine deficiency disorders surveillance
Han, WANG ; Jing, CHEN ; Ying, LI ; Ning, LIU ; Rong-hua, GUO ; Dan-na, WANG
Chinese Journal of Endemiology 2008;27(3):320-322
Objective To explore the application of clustering analysis in the study for iodine deficiency disorders(IDD)surveillance.Methods System cluster method of average linkage was applied to study the data obtained from national IDD surveilance in 2005 and a clustering analysis' tree chart was drawn.Such data as the rates of consuming qualified iodine salt,coverage rate of iodine salt,median of iodine salt,median urinary iodine,goiter rate detemined by palpation and B-uhrasonography,Child intelligence quotient were investigated.Results Thirty-one provinces or autonomous regions including the Xinjiang Production and Construction Coms had beed assorted into three types.Tibet was claasified into the type Ⅰ;Guangdong,Qinghai,Hainan,Sinkiang were assorted into the type Ⅱ,other areas were assorted into the typeⅢ.The rates of consuming qualified iodine salt,coverage rate of iodine salt and median urinary iodine in the type Ⅰ area were 21.00%,33.20%and 96.70 μg/L,while they were 71.95%,78.50%and 145.40μg/L,respectively,all being lower than that of type Ⅲ(93.20%.97.70%and 245.20μg/L)with statistical significance(P<0.05).11.50%of the children in the type Ⅰ area were detected by palpation to have goiter;the child intelligence quotient in three types of areas,was 77.00,95.95±4.46 and 104.59±6.77,the differences being statistical significant between any two types(P<0.05).Conclusion Multiple areas of IDD could be assorted by clustering analysis into distinct types with different epidemic value.More attentions of the government must be paid to the type Ⅱ areas,the prevention and cure work must be enhanced in the second-class areas while it is carried on in the typeⅢareas.The results are helpful for decision making for epidemic supervisors in the government.
7.Diagnosis and treatment of bladder benign neoplasm in children
Ying ZHANG ; Hongcheng SONG ; Ning SUN ; Weiping ZHANG ; Wenwen HAN ; Jun TIAN ; Minglei LI ; Chengru HUANG
Chinese Journal of Urology 2017;38(8):600-603
Objective To summarize the experience about the diagnosis and treatment of bladder benign neoplasm in children.Methods A retrospective study was conducted for a total of 15 patients with bladder benign neoplasm from October 2006 to May 2016.There were 10 males and 5 females with a mean age of 8.7 years (ranging 1.1-13.8 years).The clinical manifestations of 15 patients included hematuria in 9 patients,frequent micturition with urgent and painful in 3 patients,dysuria in 1 patient,abdominal pain in 2 patients and headache during voiding in 1 patient.Ultrasound showed solid masses in the bladder with iso-echoic or nonhomogeneously hypoechoic.CT scanning showed regular or irregular mass with some enhancement in the bladder.All cases received tumor complete resection by opening operation and bladders were preserved.Among the 15 cases,neoplasms located in the anterior,posterior and lateral wall of bladder in 9 cases,ureteral orifice in 4 cases and trigone of bladder in 2 cases.The size of tumors ranged from 1.2 to 6.0 cm (mean 3.1 cm).The tumors were unifocal and seemed like papillary or cauliflower.The literatures of benign neoplasm of bladder were reviewed,which focused on the clinic characters,pathological classification and therapeutic method.Results Pathologic type included papilloma in 5 patients,inverted papilloma in 1 patient,inflammatory myofibroblastic tumor in 7 patients,hemangioma in 1 patient,pheochromocytoma in 1 patient.Fifteen patients were followed up for 6 to 36 months,mean 26.7 months.All patients recovered well without relapse or metastasis Conclusions Bladder benign neoplasm in children is rare with many kinds of pathological classification.The major clinical manifestation is gross hematuria while dysuria is unusual.Tumors are fewer in trigone of bladder.The best treatment is to resect the tumor completely with bladder preservation if possible.
8.Preparation of monoclonal antibody based on single intrasplenic immunization of plasmid DNA.
Hong ZHENG ; Shuang-Yan HAN ; Ying LIN ; Xiao-Ning WANG
Chinese Journal of Biotechnology 2007;23(4):710-714
UNLABELLEDTo set up a new rapid and efficient way for the preparation of specific monoclonal antibodies (MAbs) with plasmid DNA immunization.
METHODSThe fusion gene of IL-2 signal peptide and profilin 1 by 'overlapping PCR' was obtained and inserted into the vector pVAX1 to construct the recombinant plasmid pVAX-IL2-profl. Another fusion gene of IgG kappa chain signal peptide and profilin 1 by 'no template PCR' was obtained and inserted into the vector pVAX1 to construct the recombinant plasmid pVAX-Igkappa-prof1. BALB/c mice were single intrasplenic immunized with plasmid DNA. Results After cell fusion and hybridomas screening by indirect ELISA, we charactered two mAbs (1D8A2B4 and 4B12A5E3) against profilin 1. The MAbs isotype were determined as IgM and IgG3.
CONCLUSIONA single intrasplenic plasmid DNA immunization is rapid and efficient and can be used as a helpful tool for the production of specific mAb.
Animals ; Antibodies, Monoclonal ; biosynthesis ; genetics ; immunology ; Antibody Specificity ; DNA ; genetics ; immunology ; Female ; Gene Fusion ; Immunization ; Interleukin-2 ; genetics ; immunology ; Mice ; Mice, Inbred BALB C ; Plasmids ; genetics ; Profilins ; genetics ; immunology ; Spleen ; immunology ; metabolism
9.Diagnosis and treatment of primary biliary cholangitis-autoimmune hepatitis overlap syndrome
Ning YANG ; Yansheng LIU ; Ying HAN
Journal of Clinical Hepatology 2020;36(3):697-700
Autoimmune liver diseases (AILDs) include primary biliary cholangitis (PBC), autoimmune hepatitis (AIH), and primary sclerosing cholangitis. Some patients may have the features of two AILDs at initial diagnosis or during follow-up, which is usually referred to as “overlap syndrome”, among which PBC-AIH overlap is the most common type. Compared with those with PBC or AIH alone, the patients with PBC-AIH overlap syndrome tend to have higher incidence rates of portal hypertension, gastrointestinal bleeding, ascites, death, and liver transplantation, as well as more rapid disease progression, and therefore, early diagnosis and treatment is of great importance. This article reviews the recent advances in the diagnosis and treatment of PBC-AIH overlap syndrome.
10.Analysis of highly cited papers published in Chinese Journal of Endemiology during 2000-2006
Jing, CHEN ; Dan-na, WANG ; Chun-liang, ZHU ; Rong-hua, GUO ; Yan, LIU ; Han, WANG ; Ying, LI ; Ning, LIU
Chinese Journal of Endemiology 2011;30(2):224-226
Objective To analysis the inherent quality of highly cited papers published in Chinese Journal of Endemiology, so as to identify the direction of invitation for contributions, find high quality articles, and to improve the journal's core competitiveness. Methods Scientific papers published in Chinese Journal of Endemiology from 2000 to 2006, with citation rate equal to or higher than 20 times were retrieved in China National Knowledge Infrastructure(CNKI) by December 27, 2010. These articles were statistically analyzed according to publication year, section type, author, and geographical distribution of units. Results A total of 68 highly cited papers were obtained. The citation frequency of the highly cited papers ranged from 20 to 94, with an average of 31.09 times. The highly cited papers were published in 2000 at most, accounting for 25.0%(17/68); followed by 2001,accounting for 20.6% (14/68); at least in 2006, accounting for 2.9% (2/68). Of the highly cited papers, experimental study articles was in the highest proportion of 30.9%(21/68); followed by field epidemiological investigation articles,accounting for 23.5%(16/68); editorial, review articles accounting for 10.3%(7/68) and 8.8%(6/68), respectively;short reports, case reports, meeting records etc were not highly cited papers. The number of highly cited papers in editorial, expert forums, academic contend accounted for higher proportion of total number in corresponding section types(7/19, 3/11, 3/7). Highly cited papers came from 13 provinces(municipalities), there were 10 authors contributed two or more highly cited papers, and 8 units contributed two or more highly cited papers. Conclusions Adequate space should be given to experimental research, field epidemiological investigation, editorial, expert forums andacademic contend articles due to their high rate of citation. More attention should be paid to further consolidate and expand excellent authors group, and to intensify the invitation for key issues from influential experts.