1.Clinical efficacy of magnesium isoglycyrrhizinate injection in protecting liver function for patients on chemotherapy with 5-fluorouracil
Chunshan HAN ; Lu GAO ; Lingmin HAN
Chinese Journal of General Practitioners 2013;12(4):288-289
A total of 116 hospitalized patients between May 2006 and March 2012 were divided into observation(n =39),treatment control (n =41) and blank control group (n =36).All groups received standard doses of chemotherapy with 5-fluorouracil.Patients in observation group received a daily intravenous infusion of 100 mg magnesium isoglycyrrhizinate plus chemotherapy while those in the treatment control group a daily 1.2 g reduced glutathione intravenous infusion plus chemotherapy.And the blank control group accepted chemotherapy alone.The parameters of liver function,including aspartate aminotransferase,alkaline phosphatase,gamma glutamyl transferase,lactate dehydrogenase,total bilirubin and albumin,were recorded and compared at Week 1.No significantly difference in the above parameters was found in observation group(P > 0.05).However,in treatment control and blank control groups,significant statistical differences existed in some of the parameters (P < 0.05).Magnesium Isoglycyrrhizinate is an efficient protector of liver function for patients on chemotherapy with 5-fluorouracil.
2.Reconstruction for posterior wall of external meatus and sound transmission apparatus after radical Mastoidectomy
Hongguang LU ; Han GAO ; Wei LIU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2000;(10):442-443
Objective:To reconstruct middle ear structure for open mastoid antrum with external auditorycanal after radical mastoidectomy in one-stage. Method: 71 ears of post-mastoidectomy (discharging 53 ears anddried up 18 ears) were undergone with reconstruction of middle ear. The posterior wall of external auditorycanal, mastoid cavity and chain of ossicles were reconstructed with homologous costal cartilage. Result: 69 ears of71 cases were near normal structure followed up 6 months to 5 years after operations. The result showed hearingimprovement over 15 dB were 55 ears (77.5%) and under 15 dB were 11 cases (15.5%). Five cases (7.0%)were failed. Conclusion: Reconstruction of middle ear with homologous costal cartilage is a ideal surgery toreconstruct hearing structure and avoid infection of middle cavity after radical mastoidectomy.
3.INVESTIGATION OF MATERNAL IRON STATUS IN GRAVIDA AND THE FETAL IRON STORE
Mei LU ; Wenzhen PANG ; Yuying XIN ; Yanchai GAO ; Je HAN
Acta Nutrimenta Sinica 1956;0(03):-
Eight parameters about iron nutrition were observed longitudinally in eighty pregnant women from the first trimester to delivery. Their nutrients intake were recorded at the same time. It was noted that serum iron store (ferritin) decreased markedly in the course of pregnancy and the incidence of iron deficiency increased. Total iron intake was closely related to the variation of these parameters, the degree of iron deficiency in pregnant women and the content of cord ferritin. Iron in maternal serum is correlated to ferritin in the cord blood. It suggested that natural food abundant in iron, or food fortified with iron should be taken in early pregnancy to prevent iron deficiency of mothers.
4.Spina bifida occulta in prenatal ultrasound scan
Enbo SHA ; Chunli JING ; Lu HAN ; Ziqing GAO
Chinese Journal of Perinatal Medicine 2017;20(3):190-195
Objectives To summarize and analyze the characteristics ofspina bifida occulta in prenatal ultrasound scan in order to improve the accuracy of diagnosis.Methods Sixteen singleton pregnant women with fetal spina bifida occulta who were diagnosed in the Ultrasonography Department of Dalian Maternal and Child Health Hospital from January 2014 to April 2015,were enrolled in this study.Diagnosis was made based upon the abnormalities found in the spinal column of fetus during routine ultrasound scan,followed by a close examination on the fetal spinal column and the position of conus medullaris by using multiple scan.Descriptive analysis was used to summarize the characteristics of spina bifida occulta in ultrasonic images.Results Among the 16 cases,12 had subcutaneous mass (nine cases had follicle-like anechoic mass and three cases had masses with strong echoes).The other four cases without subcutaneous mass manifested signs of disappeared physiological curvatures or disordered arrangement of spinal column,angulation deformity or scoliosis,or asymmetry in ossification centers of spinal column on both sides of lesions and lowered position of conus medullaris.Lemon shaped head and banana shaped cerebellum were seen in one case,while the other 15 cases were found no cerebral abnormalities.Eight cases had tethered cord (the position of conus medullaris was lower than the second lumber vertebra),six cases had unclear position of conus medullaris and the other two were normal.Diagnoses for all of the 16 cases were confirmed by MRI after delivery or autopsy or neonatal operation.Conclusions Abnormalities such as subcutaneous mass and tethered cord observed in prenatal ultrasound scan are significant indicators for suspected closed spinal dysraphism and further multiple scan is required to confirm the diagnosis.
5.Inlfuence of coping style and social support on quality of life in patients with impaired glucose tolerance
Jianping XU ; Han ZHOU ; Zheng LU ; Qingrong TAN ; Chengge GAO ; Dong GAO ; Xumei WANG ; Xueli SUN
Journal of Central South University(Medical Sciences) 2013;38(12):1253-1259
Objective:To explore how coping style and social support influence the quality of life in patients with impaired glucose tolerance, which act respectively as the internal and external mediating ways. Methods:A total of 283 patients with impaired glucose tolerance from 6 Three-A hospitals in China were surveyed with self-rating anxiety scale, self-rating depression scale, trait coping style questionnaire, social support scale, and WHOQOL-BREF.
Results:Biographic data failed to predict the quality of life in patients with impaired glucose tolerance, while anxiety, depression, social support and coping style significantly influenced their quality of life.
Conclusion:The fact that emotional disorder, social support and coping style influence the quality of life in patients with type 2 diabetes also exists in patients with impaired glucose tolerance.
6.GRK 2 level in peripheral blood lymphocytes of elderly patients with acute myocardial infarction
Wenqian GAO ; Chunguang HAN ; Xiaochun LU ; Yongxue LIU ; Haipeng HUI ; Hao WANG
Journal of Geriatric Cardiology 2013;(3):281-285
Objective To investigate the G protein-coupled receptor kinase 2 (GRK 2) level in peripheral blood lymphocytes with cardiac func-tion in elderly patients with acute myocardial infarction. Methods This study enrolled 40 patients with acute ST-segment elevation myo-cardial infarction (STEMI) and 40 patients with unstable angina. All patients were 65 years or older. Cardiac function was evaluated by echocardiography, and the GRK 2 level in peripheral blood lymphocytes was measured. Patients with STEMI were followed up for 2 years. Results The GRK 2 level in peripheral blood lymphocytes was significantly higher in patients with STEMI than in patients with unstable angina, and was negatively correlated with left ventricular ejection fraction, cardiac output, stroke volume, and left ventricular fractional shortening. The GRK 2 level was significantly elevated in some patients with acute STEMI and poor cardiac function. Conclusions In-creased GRK 2 level in patients with acute STEMI may contribute to poor myocardial systolic function and myocardial remodeling. Meas-urement of the GRK 2 level in peripheral blood lymphocytes may assist in the evaluation of cardiac function and myocardial remodeling in elderly patients with acute STEMI.
7.Acute toxicity of diphenylchlorarsine in mice and rats
Chuan GAO ; Xiaojing LU ; Weitao HAN ; Huifang WANG ; Jing ZHANG ; Yunyang SONG
Journal of Third Military Medical University 2003;0(20):-
Objective To assess the toxicity risks of diphenylchlorarsine (DA) to environments, human beings and livestock. Methods Totally 120 mice were randomly divided into 6 groups and given DA by gastric gavage at the doses of 32, 30, 28, 26, 24, 22 mg/kg respectively for LD_(50) of DA in mice. The toxicity of DA in rat skin were evaluated by smearing 50 ?l DA at the concentrations of 140, 70, 35, 17.5, and 8.8 mg/ml to an area of 4 cm2, and that of eyes exposure was carried out by dropping 10 ?l DA of 140.7, 70, 7, 0.7 mg/ml in rat eyes. The counterpart skin area and eyes served as control. Results LD_(50) of DA in mice was 28.07 mg/kg, the estimated doses for no-observed-adverse-effect levels (NOELs) were 8.8 mg/ml (0.44 mg) to the skin and 0.7 mg/ml (0.014 mg) to the eyes respectively. Conclusion DA can induce the injuries on the liver, kidneys, esophagus and stomach. High concentration of DA can cause inflammation of the skin and lens opacification, even blind.
8.Experimental study of WT1 specific CD8+T cells in the treatment of breast cancer
Xinchao WANG ; Suhong HAO ; Yingtang GAO ; Lijun QIU ; Shuang ZHAO ; Lu HAN
Tianjin Medical Journal 2016;44(4):397-400
Objective To investigate the feasibility of Wilms’tumor gene 1 (WT1)-specific CD8+T cells from periph?eral blood for the treatment of breast cancer by detecting the killing activity of WT1 specific CD8+T cells on breast cancer cells. Methods Flow cytometry was used to detect WT1-specific CD8+T cells in the peripheral blood of 20 samples from HLA-A2 seropositive healthy donors, which were isolated by WT1/MHC streptamer magnetic beads and cultured. The func?tion of WT1-specific CD8+ T cells were analysis by cytotoxicity assay. Results Twelve of 20 healthy donors had naive WT1-specific CD8+T-cell frequencies of>0.5%, and 4 of 20 even>1.0%of all CD8+T cells. After positive selection by magnetic cell separation, a purity of up to 80%can be achieved. WT1 specific CD8+T cells can specifically kill breast can?cer cell line with WT1 polypeptide. Conclusion WT1 specific CD8+T cells can be detected in peripheral blood of healthy volunteers. WT1 specific CD8+T cells have killing effect on breast cancer cells, suggesting the feasibility of adoptive immu?notherapy for breast cancer.
9.Clinical analysis of the treatment for benign tumor of external auditory canal by carbon dioxide laser under microscope.
Jie CHEN ; Ling LU ; Daofen QIN ; Han ZHOU ; Dengbin MA ; Jiayi LI ; Xia GAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(19):1476-1478
OBJECTIVE:
To investigate the treatment for benign tumor of external auditory canal by carbon dioxide laser under microscope.
METHOD:
Ten cases of benign tumor of external auditory canal were treated by carbon dioxide laser under microscope. The curative effects and complications were observed.
RESULT:
Ten cases of benign tumor of external auditory canal were satisfied after operation without any complications. There were no recurrences during 3 months to 2 years of follow up.
CONCLUSION
The operation for benign tumor of external auditory canal by carbon dioxide laser under microscope was easy, safe and effective.
Ear Canal
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pathology
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Ear Neoplasms
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therapy
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Humans
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Laser Therapy
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methods
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Lasers, Gas
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Microscopy
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Neoplasm Recurrence, Local
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Neoplasms
10.Expression of DLC1 mRNA and protein in breast carcinoma and non-cancerous breast tissues and its relationship with Ki-67
Yufei LIU ; Zhaoxiang ZHANG ; Hanjin DAN ; Lu CHEN ; Yiling HUANG ; Yan GAO ; Lin HAN
Chinese Journal of Clinical and Experimental Pathology 2010;(1):27-31
Purpose To investigate the expression of DLC1 and its relationship with Ki-67 in cancerous and non-cancerous tissues of the breast.Methods In situ hybridization and immunohistochemiscal EnVision method were used to detect the expression of DLC1 mRNA and protein and Ki-67 in 52 invasive breast ductal carcinomas and 42 non-cancerous mammary tissues, including 22 mammary fibroadenomas and 20 paracancerous tissues.Results The positive rates of DLC1 mRNA and protein expression in the breast carcinomas (50% and 57.7%) was significantly lower than that in the non-cancerous mammary tissues (90.5% and 92.9%) (χ~2=17.518 and 10.729,P<0.01).The expression of DLC1-mRNA was positively related to DLC1protein (r_s=0.379,P<0.01). The positive rate of Ki-67 expression was 61.5% in the breast carcinomas, but no expression was observed in the all non-cancerous tissues (χ~2=39.186,P<0.01).Correlation analysis showed that DLC1 expression was negatively correlated with Ki-67 expression (r_s=-0.507,P<0.01).Conclusions Lower or no expression of DLC1 mRNA and protein may play an important role in the pathogenesis and progression in breast carcinoma. DLC1 may inhibit the proliferation of the breast carcinoma cells,which indicates that it may act as a new molecular marker of breast carcinoma.Combining detection of DLC1 and Ki-67 may be useful parameters for evaluating the biological behaviors of breast carcinoma.