Objective To investigate the effect of Guizhi Fuling pills on levels of serum MAPK, ERK and VEGF in patients with endometriosis. Methods 60 patients with endometriosis were selected and according to the random control table were divided into the experiment group and the control group, 30 cases each.The control group were treated with conventional acetate goserelin sustained-release implantation agent subcutaneous injection, the experiment group were treated by Guizhi Fuling pill treatment.After treatment, the clinical efficacy, serum MAPK, ERK-5, VEGF, ERK, T-cad and VE-cad levels of the patients were compared.Results Compared with the control group, the clinical total effective rate was higher in the experiment group (P<0.05);MAPK and ERK-5 levels in serum were lower (P<0.05), ERK level in serum was lower(P<0.05);T-cad and VE-cad levels in serum were lower(P<0.05);VEGF level in serum was lower(P<0.05).Conclusion Guizhi Fuling pill can block the cell signal transduction pathway through inhibition of MEK and ERK activity, thereby inhibit abnormal endometrial proliferation and cell division cycle, can effectively inhibit the proliferation and differentiation of the tumor.

The need for special medical services are described and analyzed from the connotation of the characteristics of several types and models,in the hope of providing reference for the study of special need of medical services.

Objective To analyze the safety and efficacy of early diet after laparoscopic resection of colorectal cancer.Methods A prospective study of 128 patients suffered laparoscopic surgery of colorectal cancer was done in our hospital.According to whether the adoption of early diet program,patients were divided into early oral group (EOF group,n=54)and traditional oral diet group (TOF group,n=74).Age,gender,preoperative nutritional status and other basic factors and postoperative ambulation time, postoperative hospital stay time,the number of cases the use of analgesics,complications of two groups were analyzed statistically. Results The first time of postoperative ambulation time and postoperative hospital stay time of EOF group were significantly re-duced compared TOF group [(1.2±0.8)d vs .(2.5±1.3)d]and [(3.2±1.4)d vs .(5.3±1.6)d](P <0.01).Conclusion Early diet after laparoscopic resection of colorectal cancer is good for patients fast recovery,and postoperative complications of two groups are similar.

Objective To observe the effect of adenosine A1 receptor on pain and electroacupuncture analgesia, and to explore the action mechanism of electroacupuncture in analgesia.Method Adjuvant arthritis rats were taken as the study subjects. 24 rats were randomized into a normal group, a model group and an electroacupuncture (EA) group, 8 rats in each group. The pain threshold was evaluated by using thermal radiation method, immunohistochemical method and real-time fluorescence quantitative PCR were adopted to observe the expression of adenosine A1 receptor in hypothalamus and spinal cord.Result One day after modeling, the pain thresholds of right hind paw in the model group and EA group were significantly changed compared to that before modeling in the same group (P<0.01). The pain thresholds of right hind paw in the model group and EA group were significantly different from that in the normal group one day after modeling (P<0.01). 7 d after modeling, the pain threshold of right hind paw in the model group was still significantly lower than that before modeling in the same group (P<0.01), and it was significantly different from that in the normal group and EA group (P<0.01). The pain threshold was significantly enhanced in the EA group 7 d after modeling, and was significantly different from that of 1 d after modeling in the same group (P<0.01). The positive cell expression was lower in the model group and was significantly different from that in the normal group (P<0.01). The expression of adenosine A1 receptor in the EA group was markedly higher than that in the model group (P<0.01). The expression of adenosine A1 receptor in hypothalamus and spinal cord of the model group was significantly lower than that of the normal group (P<0.01). The expression of adenosine A1 receptor in hypothalamus and spinal cord of EA group was markedly higher than that of the model group (P<0.01).Conclusion EA can up-regulate the expression of adenosine A1 receptor in hypothalamus and spinal cord of adjuvant arthritis rats.

100 mm/h, group D).The tumor size/stage of the 4 ESR groups were analyzed by means of SPSS10.0. Results There were 89 cases with normal ESR,18 cases in group A,49 cases in group B,14 cases in group C and 3 cases in group D.For the correlation of ESR and tumor size,the analysis resulted in ?2 =13.621,P=0.003;Spearman correlation analysis results were:correlation coefficient of 0.341,P=0.002.For the correlation of ESR and tumor stage, the analysis resulted in ?2 =5.959 and P=0.114;Spearman correlation analysis results were:correlation coefficient of 0.208,P=0.057. Conclusions These results suggest that ESR is positively correlated with the size of renal cell carcinoma;however,there is no association between ESR and tumor stages.

Objective To discuss the effect of the teaching mode of technological pedagogical and content knowledge in biochemistry theory teaching. Methods 400 students in 8 classes of clinical medicine undergraduate in Grade 2013 were selected and randomly divided into 2 groups. The traditional teaching mode was applied in the control group of 198 students in 1-4 classes, while the technological pedagogical and content knowledge teaching mode was applied in the experimental group of 202 students in 5-8 classes. 385 students in 8 classes of clinical medical undergraduates in Grade 2014 were selected and randomly divided into 2 groups. The control group of 189 students in 1-4 classes adopted the traditional teaching mode, while the experimental group of 196 students in 5-8 classes adopted the technological pedagogical and content knowledge teaching mode, which used the micro-lesson and network platform as learning resource superior to the flipped classroom. Flipped classroom was divided into two major learning links:extracurricular self-study and class digestion. Through the network platform the micro-lesson was presented to the learners. Learners could make self-study according to their own specific circumstances and in the classroom many activities were increased such as the mutual cooperation between the students, the students' PPT teaching, the students' questions and the discussion, and the interaction between teachers and students, etc. The results of the examinations of the two terms students of the same profession and the questionnaire were analyzed. The related data were processed by SPSS 15.0, and the data between groups were compared by t test . Results The test scores analysis showed that the individual test scores in experimental group of Grade 2013 were [(17.94±2.02) vs. (12.28±4.17)], and the individual test scores in experimental group of Grade 2014 were [(18.21 ±1.78) vs. (12.45 ±5.13)], which were obviously higher than the control group, and there was statistical significance. The final exam scores in experimental group of Grade 2013 were [(78.28±11.18) vs. (68.65±12.51)], and the final exam scores in experimental group of Grade 2014 were [(81.73 ±9.12) vs. (74.41 ±11.87)], which were obviously higher than the control group, and there was statistical significance. The results of survey showed that the students thought the teaching mode aroused their study interests while 393 (93.7%), thought the teaching mode developed their self-study ability while 357 (89.7%), thought the teaching mode beneficial to cultivating their ability of solving the problems. Conclusion The teaching mode of technological pedagogical and content knowledge is of certain signifi-cance to break the plight of the traditional teaching, inspire the students interest in learning, improve the teaching quality of biochemistry, and make for the teachers' professional development.

ObjectiveTo investigate the condition of hospital staff in surgery and internal medicine department in a specified hospital for consent of patients. MethodsThis study included 50 hospital staff in surgery and internal medicine department in specified hospital at the second level in Chongqing city in May 2010. We also analyzed the dealership of children and psychopath, consent of patients or relatives for choosing hospitals. ResultsThe ratios of cognition of hospital staff for consent of patients were 68％ and 46％, which had statistical difference ( P＝0.026 ). The main ways were radio and television. About 97％ hospital staff knew that it's hospital staff s duty to inform relative information to patients. ConclusionMedical legislation department and medical institutions should enhance the study of hospital staff on relative laws and regulations. Thus hospital staff have a in-depth understanding on information of consent of patients.

Objective To investigate whether there are correlations between working memory impairmentand fractional anisotropy values and to explore the neuropathology underlying that the patients of depression suffered from memory impairment.Methods Thirty depression patients and 30 healthy controls group-matched by age,educational level were conducted the study.Mean correct reaction time(mRT)was recorded when they performed a One-Back Working Memory Task and fractional anisotropy(FA)values was recorded when they performed the diffusion tensor imaging.Statistics analysis was done respectively for mRT and FA values between two groups.Results Relative to mean correct reaction time((612.45±54.08)ms)of controls,the mean correct reaction time ((720.25±57.02)ms)of patients with depression was much longer(P＜0.05)and the patients with depression had a lower FA values in the white matter of both frontal lobe,anterior cingulate gyrus,supramarginal gyrus,splenium of corpus callosum(P＜0.05),and the FA values in the white matter of both frontal lobe were significantly negative correlated with mRT(r=-0.604,P＜0.05).Conclusion The impairment of white matter region may be one of the neuropathology underlying that the depression patients suffer from memory impairment.

The clinical effect on treatment of diabetic retinopathy with acupuncture plus herbal decoction has been observed. Eighty cases were randomly divided into treatment group and control group by the odd and even serial numbers. Forty cases in the treatment group were treated with needling Taiyang (Ex-HN 5), Yangbai (GB 14) toward Yuyao (Ex-HN 4) and Cuanzhu (BL 2) plus the herbal decoction called Mingmu Wuzi Decoction. Forty cases in the control group were treated with intravenous drip of Venorutin and oral administration of dipyridamole and vitamin E. And the result showed the total effective rate of 92.5% in the treatment group and that of 55.0% in the control group with significant difference (P＜0.01), which suggested that acupuncture plus herbal decoction had better therapeutic effect than western medications alone.

Objective A retrospective cohort study was carried out to observe the long-term effect of ESD in treating early gastrointestinal cancer or precancerous lesions. Methods The clinical and follow-up data of 73 patients were collected. Kaplan-Meier, Log-rank and Breslow test and Cox's proportional hazards regression model were used to analyze the data. Results The median survival time in the gastric and colo-rectal early cancer or precancerous lesions is longer than 65 months in our study, respectively. For esophagus, the median survival time was 44.5 months; the disease free survival time (DFS) after ESD was significantly reduced in the esophagus, compared to the stomach and colo-rectum (χ2 = 12.61, P = 0.000; χ2 = 7.09, P = 0.008); the degree of atypia (or infiltration), and lesion size were considered to be two factors to influence the DFS after ESD (P = 0.027, OR^ =2.38, 95%CI:1.10 ~ 5.12, P = 0.074, 95%CI; OR^ =0.90, 95%CI: 0.80 ~ 1.01). Conclusion ESD is an effective curative treatment in the resection of early upper gastrointestinal cancer and precancerous lesions. The degree of atypia (or infiltration) was concluded as an independent risk factor for the DFS post-ESD, and the size of lesion was a valuable parameter with regard to the recurrence after ESD procedure.