In this article,the strategy and achievements of the interdisciplinary in recent years in Peking University are summarized.We also discussed the remaining problems in the development of medical science and interdisciplinary as well as how to improve the management system and operational mechanism.

The multiplex gene expression genetic analysis system is a novel analytical platform,characterized by its primer design with gene-specific and universal sequences.The fluorescence capillary electrophoretic separation is used to compare and analyze the expression levels of multiplex PCR products.This technology is simple,rapid,productive and cost-effective,and holds great promise for cancer classification and biomarkers discovery.

Objective To investigate the adverse reactions influence factors of deep sedation anesthesia.Methods 120 cases with elective gastrointestinal tumor resection were chosen and given deep sedation anesthesia.The occurrence of intranperative bemodynamic changes at each time point and adverse reaction were statistically analyzed.Results SBP,DBP,HR and SpO2 values at different anesthesia time point had significant differences (F =9.688,10.258,7.586,8.992,all P =0.000).26 cases had intraoperative and postoperative adverse reactions,the incidence was 21.7％,8 cases with hypotension,4 cases with bradycardia,6 cases with nausea and vomiting,2 cases with apnea,6 cases with other adverse reactions.No death and serious adverse reactions occurred.Senility,depth anesthesia application history,psychological stress and obesity were risk factors of intraoperative and postoperative adverse reactions of gastrointestinal tumor by deep sedation anesthesia(OR values =9.806,0.119,0.105,10.521,all P ＜0.05).Conclusion Adverse reaction incidence of gastrointestinal tumor by deep sedation anesthesia was high,and more performance for hemodynamic response,anesthesiology doctors could improve patients prognosis by regulating the perioperative risk factors.

Dyslipidemia plays an important role in the pathogenesis of diabetic retinopathy (DR).A preliminary study found that low-density lipoprotein cholesterol,apolipoprotein (Apo) B and Apo B / Apo A1 ratio were positively correlated with DR,while high-density lipoprotein cholesterol,Apo A1 was negatively correlated with DR and proliferative DR.Reducing the blood fats to be helpful to DR control.However,the mechanism of hyperlipidemia in the pathogenesis of DR,the reason of dyslipidemia in diabetic patients and the interaction between hyperglycemia and hyperlipidemia in DR are not clear yet.Moreover,there is no predictive indicators related to blood lipid for DR.Understanding the relationship between dyslipidemia and DR can provide definite evidence for fat-reducing therapy for DR control.

The dark-adapted efectroretinogram (ERG) was investigated in 67 diabetic patients (124 eyes) and in 50 normal subjects (75 eyes). The results showed that the rate of reduced amplitudes and lengthened peak times of a, b waves, as well as the rate of extingaished oscillatory potentials in diabetics ERG were elevated in sequence from no, mild, moderate to severe retinopathy (P

Objective To explore the complications and the treatments of repeated cesarean delivery in scar uterus pregnancy accompanied by the placenta praevia. Methods We performed a retrospective study in 6832 cases underwent first cesarean delivery,within which 201 cases were accompanied by the placenta praevia;and 337 cases underwent repeated cesarean deliver, within which 26 cases were accompanied by the placenta praevia. All subjects accepted cesarean delivery from January2006 toApril 2010 in our hospital. Results The occurrence rate of placenta previa was significantly higher in the repeated cesarean delivery than first-ever cesarean delivery (7. 72% vs 2. 94%, x2 = 22. 33, P ＜ 0. 01 ) , especially the occurrence of complete placenta previa (42. 30% vs 0. 00%, x2 = 80. 43, P ＜ 0. 01 ). The rates of uterus rupture, placenta accreta, postpartum hemorrhage and hysterectomy (r = 26. 92% ,23.08% ,26. 92% and 7. 69%, respectively) in repeated cesarean delivery in scar uterus pregnancy accompanied by the placenta praevia were significantly higher than those of the cases ( r = 2. 57% ,0. 32%, 1.29% and 0. 00%, respectively ) had repeated cesarean delivery in scar uterus pregnancy but no placenta previa ( x2 = 27.97,50. 41,42. 16,12. 79, respectively, Ps ＜ 0. 01 ). Conclusion The incidence of placenta previa increased in scar uterus pregnancy, especially the complete placental previa.Scar uterus pregnancy accompanied by the placenta praevia are more likely to occur uterus rupture,placenta accreta,postpartum hemorrhage and had hysterectomy. Obstetricians should give more effort to reduce the cesarean section rate,improve the quality of medical care.

Objective To analyze the risk factors for traumatic cerebral venous sinus occlusion (CVSO)and to investigate the strategies of early diagnosis of traumatic CVSO. Methods The clinical data of 212 patients with moderate to severe closed traumatic brain injury from January 2012 to December 2015 were analyzed retrospectively. Logistic regression analysis was used to evaluate the risk factors for traumatic CVSO. Results Of the 212 patients with traumatic brain injury, 16.5%(35/212) patients had CVSO. Ten patients had CVSO of thrombotic type (typeⅠ), 16 patients had CVSO of compression type (typeⅡ), and 9 patients had CVSO of mixed type (typeⅢ). Logistic regression analysis showed that skull fracture (OR = 8.141; 95%CI: 3.224-20.840) and epidural hematoma of crossing venous sinus (OR = 3.179; 95%CI: 1.470-7.037) were the risk factors for CVSO, and the former was more significantly correlated with CVSO. Female gender was the risk factor for CVSO typeⅠ(OR =10.425; 95%CI: 1.831-30.053), epidural hematoma of crossing venous sinus was the risk factor for CVSO typeⅡ(OR = 5.766; 95%CI: 1.885-18.197), and skull fracture, epidural hematoma of crossing venous sinus, and the previous history of vein thrombosis was the risk factors for CVSO type Ⅲ(OR =18.005, 4.596, 11.394; 95%CI: 2.021-58.836, 1.144-19.525, 1.436-46.558). Conclusions In the early diagnosis of traumatic CVSO, the crossing venous sinus fracture line and epidural hematoma should be given attention. Attention should be paid to the history of venous thrombosis. MR venography and CT venography contributes to early diagnosis of CVSO.

Objective: To discuss the feasibility of a new method to evaluate wearing simulators,and to investigate the feasibility of specimens' depth loss as the index of the materials' wearing resistance.Methods: Two commercial composite resins(SureFil and Spectrum TPH) were selected.The specimens were subjected to 100,200,300,400,500,800,1200,1 600 and 2 000 cycles wearing in the CW3-1 wearing system.Wearing was determined quantitatively by weighing the specimen and measuring the height of the specimen,volume loss(mm 3) and height loss(?m) were calculated.Previous clinical study results on SureFil and Spectrum TPH conducted at Hong Kong University were used to examine the relationship between clinical wearing and laboratory wearing.The difference of the wearing resistance between the two materials and the Pearson correlation of the height loss and the volume loss were analyzed by statistical software SPSS 15.0.Results: The wearing resistance of the two materials was significant difference(P

Objective To investigate the effects of compound lidocaine cream applied to tracheal tube on the recovery of tonsil with adenoidectomy in general anesthesia. Methods 120 patients undergoing elective tonsillectomy with adenoidectomy were randomly divided into the experimental group and the control group. In the experimental group, the surface of the tracheal tube was uniformly coated with compound lidocaine cream before anesthesia. In the control group, paraffin oil was given evenly to the surface of the tracheal tube. The changes of hemodynamics and the incidence of complications in the two groups were observed and recorded. Results The indexes of hemodynamics in the experimental group were more stable than those in the control group, and the control group had poor tube resistance, and the incidence of complications such as cough, irritability and sore throat was increased (P<0.05). Conclusion Application of compound lidocaine cream on tracheal tube surface can effectively reduce the cough and other stress response during the general anesthesia recovery period, decrease postoperative irritability and improve the safety of children after surgery.

Objective:Previous genome-wide microarray analysis found TTCATTCT motif(DNA binding site of Runx1)in the RPL4 promoter of childhood leukemia cells,suggesting that Runx1 may regulate the expression of RPL4.This study is to investigate the relationship between Runx1 and RPL4 so as to better understand the effects of Runx1 on RPL4gene transcription,laying a foundation for further studying leukemogenesis of childhood leukemia.Methods:The luciferase plasmids containing RPL4 promoter and its mutant were constructed and were co-transfected into 293T cells with the expression plasmid of Runx1.The transactivity of RPL4 promoter was assayed by luminometer.Results:Runx1 significantly decreased the transcriptional activity of RPL4 promoter(P0.05).Conclusion:Runx1 can inhibit RPL4 gene transcription in a dose-dependent manner through binding to the TTCATTCT motif.