Objective:To explore the effects of combination of epirubicin (EPI) and anti-CD47 antibody on therapy of breast cancer.Calreticulin ( CRT) and CD47 on DC-mediated phagocytosis.Methods: Breast cancer cells MCF-7 and MDA-MB-231 were treated with EPI , and expression of calreticulin ( CRT ) and CD47 on cell surface were detected by flow cytometry or immunofluorescence.DC were isolated from human peripheral blood , and cocultured with epirubicin-treated breast cancer cells in the present of anti-CD47 or anti-CRT antibody ,and the phagocytosis of DC was assessed.Results:Untreated breast cancer cells MCF-7 and MDA-MB-231 did not express CRT on the surface of cells ,but high level of CD47 was detected.After EPI treatment,CRT exposed on the cells surface,and the expression of CD47 was reduced.The results showed that EPI or anti-47 antibody alone increased DC-mediated phagocytosis ,and combination treatment marked enhanced this effects.Further more, in addition of anti-CRT antibody reduced the phagocytosis of DC.Conclusion:EPI could induce CRT exposure on the tumor cell surface ,and enhanced DC-mediated phagocytosis when combination with anti-CD47 antibody.The phagocytosis of DC on tumor cells were modulated by both ecto-CRT and CD47.This study provide a new effective strategy for the use of anti-CD47 antibody among the chemotherapy of breast cancer.

OBJECTIVETo explore the clinical effect of Erlong Xizhu acupuncture for retinitis pigmentosa. Methods Sixty patients with retinitis pigmentosa were randomly divided into an Erlong Xizhu acupuncture group and a conventional acupuncture group, 30 cases in each one. In the conventional acupuncture group, Tongziliao (GB 1), Sizhukong (TE 23), Taiyang (EX-HN 5) were acupunctured as the main acupoints by uniform reinforcing-reducing manipulation; acupoints based on syndrome differentiation were coordinated by acupuncture manipulation of reinforcing deficiency and reducing excess. The treatment in the Erlong Xizhu acupuncture group was the same as that in the conventional group, except acupuncture manipulation at the main acupoints replaced by Erlong Xizhu acupuncture. In the two groups, treatment was given once a day, 10 days as one session and there were 2 days at the interval between sessions. The change of the vision and visual field and the efficacy were observed after 3 sessions in the two groups.

RESULTSThe total effective rate on enhancing the vision of patients in the Erlong Xizhu acupuncture group was 93. 10% (54/58), which was superior to 81. 36% (48/59) in the conventional acupuncture group (P<0. 01). And the effective rate on improving the visual field was 91. 38% (53/58) in the Erlong Xizhu acupuncture group, which was better than 83. 05% (49/59) in the conventional acupuncture group (P<0. 01). On the comprehensive effect, the effective rate was 90. 00% (27/30) in the Erlong Xizhu acupuncture group, which was better than 63. 34% (19/30) in the conventional acupuncture group (P<0. 05).

CONCLUSIONThe clinical effect of Erlong Xizhu acupuncture is superior to that of conventional acupuncture for retinitis pigmentosa and it is worthy of promoting.

Acupuncture Points ; Acupuncture Therapy ; methods ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Retinitis Pigmentosa ; physiopathology ; therapy ; Treatment Outcome ; Vision, Ocular ; Young Adult

ObjectivesTo study the current status of child neglect and its inlfuencing factors in children aged 3-6 years in rural Henan.MethodsScales and evaluation methods in the Chinese rural child aged 3-6 years neglected eval-uation model were applied in this study. The neglect rate and the neglect degree were used to describe the neglect status of rural children aged 3-6 years in Henan. The inlfuencing factors for child neglect were also analyzed. ResultsThe total neglect rate in 450 enrolled children was 37.1% (167/450). The total neglect degree was 51.9±6.5. The boys had signiifcant-ly higher neglect rate than girls (P<0.05). There was no significant difference in neglect degree between boys and girls (P>0.05). There were significant differences in neglect rate and neglect degree among the different age groups (P<0.05), the higher neglect rate and higher neglect degree over age. The neglect rate and neglect degree in nuclear families and fam-ilies with three generations were lower than those in single-parent families and remarried families (P<0.05). The neglect rate and neglect degree in non-only children were higher than those in only children (P<0.05). The left-behind children had signiifcantly higher neglect rate and neglect degree than children living with parents (P<0.05). Based on multivariate logis-tic regression analysis, the risk factors of child neglect were single-parent families (OR = 4.78, 95%CI : 2.10-10.87) and remarried families (OR=4.53, 95%CI : 1.99-10.01). The protective factors of child neglect were parents working on sci-ence (OR = 0.20, 95%CI : 0.10-0.67) and living with parents (OR = 0.03, 95%CI : 0.00-0.54).ConclusionsThe neglect status is serious in Henan rural children, particularly in left-behind children.

A semantic predication network was developed by processing the documents on schizophrenia into se-mantic predication sets using SemRep, from which the core information was extracted to produce a graphic summary which was consisted of highly cohesive cliques.The automatic methods for summarizing biomedical documents were studied using the network properties combined with semantic information.The subthemes in the summary obtained by clustering were evaluated according to the clique co-node matrix and the contents of the summary were assessed according to the reference criteria.The accuracy was 0.93, the recall was 0.68, and the F-value was 0.79 for the summary, indicating that this method can effectively recognize the core information in documents and the semantic information in network graphics.

Cycloxygenase-2 plays an important role in many human tumorigenesis, and is overexpressod in breast cancer. Therefore, it is necessary to investigate the relationship between COX-2 and breast cancer, and to develop the desirable COX-2 inhibitor. These can privide a new antitumor method.

Objective To analyse subtyping of iscbemic stroke by the criteria of Trial of Org 10 172 in Acute Stroke Treatment (TOAST), its distribution, and relationship between TOAST subtypes, its risk factors and recurrence. Methods Clinical data of 441 patients of ischemic stroke were collected prospectively during October 1, 2006 to September 30, 2007. All the patients were classified by TOAST criteria into five major subtypes and followed-up for one year since its first episode. Dates of its recurrence and death due to recurrence were recorded. Results All the 441 patients with ischemic stroke could be subtyped etiologically according to TOAST criteria as follows: etiology undetermined in 42.3 percent, small-vessel occlusion in 30.9 percent, large-artery atherosclerosis in 17.3 percent, cardioembolism in 9.3 percent, and others in 0.2 percent, without statistically significant difference in its gender and age distributions. Recurrence rate of iscbemic stroke in one year since its first episode for varied subtypes was 33.3 percent for cardioembolism, 14. 6 percent for etiology undetermined, 13.7 percent for large-artery atherosclerosis and 6.9 percent for small-vessel occlusion, respectively. There was no significant difference in risk factors for varied subtypes of iscbemic stroke, including history of smoking, hypertension, diabetes, cardiac disease, and serum levels of total cholesterol, low-density lipoprotein cholesterol, fihrinogen and glucose. Conclusions Varied subtypes of iscbemic stroke had different risk factors and recurrence rate by TOAST criteria, which can be used as an etiologic classification for its secondary prevention.

The immune tolerance or immune escape plays important roles in the genesis and progression of malignant gynecological neoplasms.Human leukocyte antigen G(HLA-G)is an important member of major histocompatiblity complex class Ⅰ.HLA-G seems to affect almost every aspect of human immunity.It can inhibit immunological function,and play an important role in immune escape of malignant gynecological neoplasms.

Clinicians should assume the responsibility of nutritional therapy for satisfactory outcomes. They should master knowledge on nutritional metabolism, and participate in the administration of nutritional support. Clinicians should be acquainted with new concepts and standardized techniques in this field, such as different requirement of energy, understanding of early nutritional support, and optimal regimens of enteral or parenteral nutrition.
China ; Enteral Nutrition ; Humans ; Nutritional Support ; Parenteral Nutrition

80 years)in China is increasing.As a special type of hypertension,the hypertension in the very elderly has some specificities in pathogenesis,clinical manifestation,treatment and prognosis.The topic to effectively cure and prevent hypertension in the very elderly is increasingly emphasiced.This article presents an overview of recent progress in clinical characteristics,and treatment strategy related to hypertension in the very elderly.

With the development of cartilage tissue engineering, how to achieve an ideal scaffold has become the difficult and hot spot in this study field. The scaffold for cartilage tissue engineering should provide with some special physical and biochemical properties, such as excellent biocompatibility, proper biodegradability, controlled porosity, adequate pore size and so on. Such properties are closely related to two factors, one is the materials used for scaffolds, the other is the fabrication technology. This review is concentrated on the present common fabrication technologies of three-dimensional porous scaffolds, and mainly introduces the following technologies: solvent casting/particulate leaching, phase separation/freeze-drying, hydrogels, gas foaming, electrospinning and rapid prototyping manufacturing. Those fabrication technologies' effects on structure and properties of scaffolds have been compared to summarize and analyze the advantages and disadvantages of every fabrication technology, and simultaneously prospect the developing tendency of fabrication technology in cartilage tissue engineering.