Aim To investigate the effects of com-bined treatment of SAHA and TRAIL on human breast cancer ER positive cell line MCF-7 . Methods MCF-7 cells were treated with SAHA and/or TRAIL. The inhibitory rates were detected by real-time cell prolifer-ation assays. Morphology changes of MCF-7 cells were observed through time-lapse live cell imaging acquisi-tion. Results Real-time cell proliferation assays showed that the anti-tumor efficacy of SAHA was sig-nificantly enhanced in combination with TRAIL. The results of time-lapse live cell imaging acquisition dem-onstrated that, with treatment of SAHA and TRAIL, the growth inhibition of MCF-7 cells was more obvious than that of in TRAIL or SAHA treatment alone. Con-clusion The combination treatment of SAHA and TRAIL has a synergistic effect of growth inhibition on breast cancer MCF-7 cells.

Objective To analyze the effects of gentongping pill combined with physiotherapy on the patients with vertebral artery type of cervical spondylosis. Methods 134 patients with vertebral artery type of cervical spondylosis were grouped into two groups, the observation group and the control group. The two groups were treated with physiotherapy and the observation group was treated with Gentongping pill. The effects in the two groups were evaluated. Results The total efficiency in the observation group was higher than that in the control group(P<0.05). After treatment, the symptom score, CASCS score, mean blood flow velocity in left vertebral artery and right vertebral artery, TNF-α and IL-6 level in the observation group were better than those in the control group (all P<0.05). There was no obvious adverse reaction in the two groups. Conclusion Itcan improve the therapeutic effect which Gentongping pill is used on the treatment of patients with vertebral artery type of cervical spondylosis.

The medical equipment benefit evaluation analysis has become an important constituent of cost and medical equipment management. It can not only use the economic benefit index to estimate the service condition of equipment, but also form the comprehensive evaluation system with basic of economic benefit evaluation and auxiliary of science study. Based on comprehensive evaluation result, to search the weakness of equipment utilization and promote the overall development of hospitals and clinics orderly.

The multilateral trading system embodied in the World Trade Organization has contributed significantly to economic growth, development and employment throughout the past fifty years. We are determined, particularly in the light of the global economic slowdown, to maintain the process of reform and liberalization of trade policies, thus ensuring that the system plays its full part in promoting recovery, growth, and development. The negotiations on trade in services shall be conducted with a view to promoting the economic growth of all trading partners and the development of developing and least-developed countries. The General Agreement on Trade in Services (GATS) is among the World Trade Organization's most important agreements. The accord, which came into force in January 1995, is the first and only set of multilateral rules covering international trade in services. Ig has been negotiated by the Governments themselves, and it sets the framework within which firms and individuals can operate. The GATS has two part: the framework agreement containing the general rules and disciplines ; and the notional "schedules" which list individual countries' specific commitments on access to their domestic markets by foreign suppliers. Each WTO Member lists in its national schedule those services for which it wishes to guarantee access to foreign suppliers. All commitments apply on a non-discriminatory basis to all other Members. There is complete freedom to choose which services to commit. In addition to the services committed, the schedules limit the degree to which foreign services providers can operate in the market. For example, a country making a commitment to allow foreign banks to operate in its territory may limit the number of banking licenses to be granted (a market access limitation). It might also fix a limit on the number of branches a foreign bank may open (a national treatment limitation). The GATS covers all internationally-traded services with two exception : services provided to the public in the exercise of governmental authority, and , in the air transport sector, traffic rights and all services directly related to the exercise of traffic rights and all services directly related to the exercise of traffic rights. The GATS also defines four ways in which a service can be traded, known as "modes of supply" Services supplied from one country to another (e.g international telephone calls), officially known as "cross-border supply"; Consumers from one country making use of a service in another country(e.g tourism), officially known as "consumption abroad"; A company from ton country setting up subsidiaries or branches to provide services in another country (e.g a bank from one country setting up operations in another country), officially known as "commercial presence" ; and Individuals travelling from their own country to supply services in another (e.g an actress or construction worker), officially known as "movement of natural persons". Trade liberalization, and even economic growth, are not the ends in themselves. The ultimate aim of Government is to promote human welfare in the broadest sense, and trade policy is only one of many instruments Governments use in pursuing this goal. But trade policy is nevertheless very important, both in promoting growth and in preventing conflict. The building of the multilateral trading system over teh past 50 years has been one of the most remarkable achievements of international cooperation in history. The system is certainly imperfect, which is one of the reasons why periodic negotiations are necessary, but the world would be a far poorer and more dangerous place without it. In January 2000, WTO Member Governments started a new round of negotiations to promote the progressive liberalization of trade in services. The GATS agreement specifically states that the negotiations "shall take place with a view to promoting the interests of all participants on a mutually advantageous basis" and "with due respect for national policy objectives and the level of development of individual Members". The pace and extent of these negotiations are set by the WTO's 140 Member Goverments themselves according to their different national policy priorities. Recently, however, the negotiations and the GATS itself have become the subject of ill-informed and hostile criticism. Scare stories are invented and unquestioningly repeated, however implausible. It is claimed for example that the right to maintain public services and the power to enforce health and safty standards are under threat, though both are explicitly safeguarded under the GATS. How have serious people come to believe what is, on the face of it, out of the question? Why should any Government, let alone 140 Governments, agrees to allow themselves to be forced, or force each toher, to surrender of compromise powers which are important to them, and to all of us? Decision-making in open societies presupposes informed public discussion. It must be based on fact rather than fiction. The purpose of this booklet is to contribute to this discussion and to a greater public understanding of the GATS by correcting statements made in some recent publication which we believe are misleading the public and undermining support for international economic cooperation. It must not be assumed that becuase we have disputed some allegations we accept that others are well-founded: these are merely examples.
Appointments and Schedules ; Delivery of Health Care* ; Economic Development ; Employment ; Financing, Organized ; Freedom ; Humans ; International Cooperation ; Licensure ; Linear Energy Transfer ; Negotiating* ; Pamphlets ; Publications ; Telephone

Based on the imaging photoplethysmography (iPPG) and blind source separation (BSS) theory the author put forward a method for non-contact heartbeat frequency estimation. Using the recorded video images of the human face in the ambient light with Webcam, we detected the human face through software, separated the detected facial image into three channels RGB components. And then preprocesses i.e. normalization, whitening, etc. were carried out to a certain number of RGB data. After the independent component analysis (ICA)'theory and joint approximate diagonalization of eigenmatrices (JADE) algorithm were applied, we estimated the frequency of heart rate through spectrum analysis. Taking advantage of the consistency of Bland-Altman theory analysis and the commercial Pulse Oximetry Sensor test results, the root mean square error of the algorithm result was calculated as 2. 06 beat/min. It indicated that the algorithm could realize the non-contact measurement of heart rate and lay the foundation for the re- mote and non-contact measurement of multi-parameter physiological measurements.
Algorithms ; Face ; Heart Rate ; Humans ; Monitoring, Physiologic ; methods ; Oximetry ; Photoplethysmography ; Software ; Video Recording

Inflammatory bowel disease,a type of intestinal chronic inflammatory disease caused by multi-factors,which include ulcerative colitis,Crohn's disease and inflammatory bowel disease unclassified.The morbidity of inflammatory bowel disease has been increasing by years with a younger age trend.Nowadays the pediatric inflammatory bowel disease is prevalent.Pediatric inflammatory bowel disease is different from adult at many aspects such as clinical manifestation,disease type,treatment,complications etc.It is because children are in a special condition with fast growth and development.This article will elaborate the current status of treatment and progresses of inflammatory bowel disease especially pediatric inflammatory bowel disease.

OBJECTIVE:To investigate the regulation effects of oleanolic acid on the mitochondrial nitric oxide synthase(mt-NOS) in human umbilical vein endothelial cells (HUVECs) with oxidative damage. METHODS:HUVECs in exponential phase were divided into normal group,model group and oleanolic acid low,medium and high dose groups(5,20 and 35 μmol/L). After drug acting for 24 h,all groups were given culture solution containing 100 μg/ml ox-LDL to reproduce oxidative damage except normal group. CCK-8 was used to detect cell viability. The mitochondria in cells were extrated,enzyme chemical method was used to detect mtNOS activity and mtNO content,fluorescence microplate method was used to detect fluorescence intensity of reactive oxygen species(ROS),and western blot was used to detect expression of cytochrome C(Cyto-C). RESULTS:Compared with nor-mal group,the cell viability in model group was decreased;mtNOS activity,mtNO content,ROS fluorescence intensity and Cy-to-C protein expression were increased,with significant differences (P<0.05). Compared with model group,the cell viability in oleanolic acid low,medium and high dose groups was increased;mtNOS activity,mtNO content,ROS fluorescence intensity and Cyto-C protein expression were decreased,with significant differences(P<0.05),and they had positive correlation with concentra-tions. CONCLUSIONS:Oleanolic acid can decrease the mtNOS activity of HUVECs,reduce the production of mtNO and Cyto-C, by a mechanism that may be related to the decrease of ROS expression.

Constitution is an important factor to determine the susceptibility and the occurrence of disease. This article analyzed causes and characteristics of damp-heat constitution, the change of damp-heat constitution to damp-heat syndrome, the relationship between damp-heat constitution and the occurrence and development of diabetes mellitus. It reached the conclusion that damp-heat constitution is one of the important factors of diabetes mellitus, that damp-heat syndrome is always accompanied with the development of diabetes, and proposed the treatment methods of lifestyle intervention, elimination of pathogens through purgation and diuresis, nourishing yin and dredging the collaterals. It also proposed the new ideas and thoughts of early intervention in damp-heat constitution and distinguishing constitution therapy for the prevention and treatment of diabetes mellitus.

Contrast induced nephropathy is an important complication arising from use of iodinated contrast media.Such iatrogenic complication may result in poor prognosis and additional health care costs.Cases of contrast induced nephropathy are on the rise along with the-increasing number of contrast-requiring procedures.Contrast induced nephropathy has been a subject of concern to cardiologists in recent years.Several large series have shown no approved methods for management of periprocedural contrast induced nephropathy.However,identification of high-risk patients,improvement and rectification of related risk factors,use of iodinated contrast media with less renal toxicity,and sufficient hydration may reduce the incidence of contrast induced nephropathy.

Objective To design an intravenous indwelling needle in battle field aid.Methods Several concavity were made on the hollow tube inner surface posterior puncture needle case,and some corresponding convexity uplifted peripheral needle handle pistons posterior puncture needle core.Revolution could be avoided because the needle handle pistons were just embedded in hollow tube concavity.It was convenient to operate and decreased the operator's orientation transformation by the needle's handhold vane adopting rectangle instead of butterfly shape.Results The improved puncture needle was stable and the rate of success of intravenous puncture was increased.Conclusion The improved venous retaining needle can be applied in battle field aid.