To explore the feasibility of sevoflurane used in obstetric anesthesia and examine its effects on neonatal Apgar scores.A total of 60 cases of term primipara were assigned into epidural anesthesia assisted sevoflurane inhalation group (experimental group,n =30) and epidural anesthesia group (control group,n =30).In the experimental group after skin incision,the patients could not tolerate the anesthesia mask inhalation of sevoflurane.And the control group received no adjuvant drug.The maternal inhalation of sevoflurane to delivery of fetus after 1 min,5 min,10 min Apgar scores were analyzed.And the inhalation of sevoflurane to fetal childbirth duration were 4-10 min in two groups.The intergroup difference of newborn Apgar scores had no statistical significance (P ＞ 0.05).Epidural anesthesia assisted sevoflurane inhalation for cesarean section had no significant effect on neonatal Apgar scores.

OBJECTIVE: To put forward proposals for improving essential medicines system in China.METHODS: The problems existing in the essential medicine system in China and the successful experience of some other countries were analyzed.RESULTS & CONCLUSION: Considering the current medical economy level and the capacity on research & development(R&D) of drugs,China should draw inspiration from WHO and other developed countries about their successful experiences on essential drugs to improve the screening methods for essential medicines list,assign legal status for essential medicines and build up system of production,procurement and distribution of essential medicines according to the basic national situation of our country and finally establish the essential medicine system suitable to our country's condition.

BACKGROUND: Much research focuses on the link between ?-amyloid peptide and neuron death, but there is little work about white matter alterations in the Alzheimer’s disease. OBJECTIVE: To investigate the anterior commissure pathological alteration in the APP/PS1 transgenic mice which model brain amyloidosis of Alzheimer’s disease. DESIGN, TIME AND SETTING: A grouping observational study based on the histology was performed in the Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology between September 2007 and September 2008. MATERIALS: Female transgenic APP/PS1 mice [Thy1 APP751 SL (Swedish mutation KM670/671NL, London mutation V717I introduced in human sequence APP751) ? human mutation gene PS1 M146L], control animals were amyloid-deposit free female PS1 mice. A total of 28 mice were divided into young group (2 months, 8 APP/PS1, 7 PS1) and old group (24 months, 6 APP/PS1, 7 PS1). METHODS: The slides of brain tissue were stained with Congo red and antibody against amyloid beta (4G8) to detect brain amyloidosis in Alzheimer’s disease transgenic model. Myelin was stained with gold chloride and axon was stained with anti-neurofilament M antibody. The anterior commissure axonal density and myelination were quantitatively analyzed with the relative optical density value of staining with densitometry. MAIN OUTCOME MEASURES: The staining of intracellular and extracellular amyloid beta; ②the average area of anterior commissure in the coronal brain tissue sections; ③the relative optical density value of myelin and axon staining in the anterior commissure. RESULTS: A lot of Congo red positive amyloid beta plaques were observed in the cortex, hippocampus, thalamus, and anterior commissure of aged APP/PS1 mice, while intracellular amyloid beta was only present in the cortex of young APP/PS1 mice. A prominent increase in the surface area of the anterior commissure was observed in aged PS1 mice compared with young PS1 mice and aged APP/PS1 mice. The neurofilament staining remarkably decreased, both in aged APP/PS1 and aged PS1 mice; an increase trend of myelination in the anterior commissure was observed both the forementioned groups. Different phenotype analysis demonstrated that axonal density and myelination was comparative in the young APP/PS1 and young PS1 mice; axonal density of aged APP/PS1 mice decreased remarkably compared with aged PS1 control mice, while myelination of aged APP/PS1 mice had no significant difference with aged PS1 mice. CONCLUSION: There exists an axon loss in the anterior commissure in the aged APP/PS1 mice with a complete myelin sheath. The amyloid beta shows a direct toxicity on the axon.

BACKGROUND: Much research focuses on the link between β-amyloid peptide and neuron death, but there is little work about white matter alterations in the Alzheimer's disease.OBJECTIVE: To investigate the anterior commissure pathological alteration in the APP/PS1 transgenic mice which model brain amyloidosis of Alzheimer's disease.DESIGN, TIME AND SETTING: A grouping observational study based on the histology was performed in the Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology between September 2007 and September 2008.MATERIALS: Female transgenic APP/PS1 mice [Thy1 APP751 SL (Swedish mutation KM670/671NL, London mutation V7171 introduced in human sequence APP751) × human mutation gene PS1 M146L], control animals were amyloid-deposit free female PS1 mice. A total of 28 mice were divided into young group (2 months, 8 APP/PS1, 7 PS1) and old group (24 months, 6 APP/PS1, 7 PS1).METHODS: The slides of brain tissue were stained with Congo red and antibody against amyloid beta (4G8) to detect brain amyloidosis in Alzheimer's disease transgenic model. Myelin was stained with gold chloride and axon was stained with anti-neurofilament M antibody. The anterior commissure axonal density and myelination were quantitatively analyzed with the relative optical density value of staining with densitometry.MAIN OUTCOME MEASURES: ①The staining of intracellular and extracellular amyloid beta; ②the average area of anterior commissure in the coronal brain tissue sections; ④the relative optical density value of myelin and axon staining in the anterior commissure.RESULTS: A lot of Congo red positive amyloid beta plaques were observed in the cortex, hippocampus, thalamus, and anterior commissure of aged APP/PS1 mice, while intracellular amyloid beta was only present in the cortex of young APP/PS1 mice. A prominent increase in the surface area of the anterior commissure was observed in aged PS1 mice compared with young PS1 mice and aged APP/PS1 mice. The neurofilament staining remarkably decreased, both in aged APP/PS1 and aged PS1 mice; an increase trend of myelination in the anterior commissure was observed both the forementioned groups. Different phenotype analysis demonstrated that axonal density and myelination was comparative in the young APP/PS1 and young PS1 mice; axonal density of aged APP/PS1 mice decreased remarkably compared with aged PS1 control mice, while myelination of aged APP/PS1 mice had no significant difference with aged PS1 mice.CONCLUSION: There exists an axon loss in the anterior commissure in the aged APP/PS1 mice with a complete myelin sheath. The amyloid beta shows a direct toxicity on the axon.

Objective To establish a safe and effective method for obtaining fetus of rhesus monkey.Methods The pregnant process of rhesus monkey was monitored by B ultrasonography,and the pregnant rhesus monkeys in different stages of pregnant were randomly chosen.Mifepristone was subcutaneously injected to pregnant rhesus while misoprostol was put to their posterior fornix.The efficacy of this method was evaluated by the results of bishop score,the rate of pregnancy termination and the quality of total RNA extracted from brain samples of rhesus fetus.ResultsThe scores of bishop score and rates of fetus delivery of experiment group were both higher than that of control group(P

Objective: To examine the correlation of symptomatic feature and exploratory eye movements in schizophrenia. Methods: The eye movements of 60 patients with schizophrenia and 30 healthy control subjects were measured by the eye-mark recorder (model IV, Japan) and symptom profiles of patients were assessed using the Positive and Negative Syndrome Scale (PANSS) . Pearson correlation analysis was performed to examine the relationship between five-factor scores of PANSS, positive symptoms, negative symptoms and exploratory eye movements respectively. Results: The differences of all indices of eye movements between schizophrenic groups and normal control group had statistical significance ( P

Objective To explore the effect of analgesia management on the analgesic effect and the quality of delivery of labor analgesia. Methods The 80 cases were divided into two groups according to the odd number of labor analgesia, the control group without anesthesia nurse labor analgesia, only the anesthesiologist operation, given routine obstetric care. Anesthesia nursing group for labor analgesia by anesthesiologists and nurse by anesthesia on maternal perinatal period health education and pain management, pain score was compared between the two groups (at full dilatation), mode of delivery, postpartum hemorrhage, neonatal asphyxia. Results The total score of pain in the nursing group was 3.9±0.2, while that of the control group was 8.4±0.8, and the difference was statistically significant (t=-38.5872,P=0.000); anesthesia nursing group cesarean delivery, delivery, were 1 cases, 27 cases, 12 cases; the control group were 9 cases, 21 cases, 10 cases, the difference was statistically significant (χ2=7.31, P<0.05) ; the amount of bleedingof anesthesia nursing group (175.5±42.5) ml; the control group was (226.4±52.5) ml, the difference was statistically significant (t=8.621, P<0.05); the incidence of neonatal asphyxia anesthesia nursing group 1 cases (2.5%); the control group was 3 cases (7.5%), the difference was not statistically significant (χ2=0.263, P>0.05). Conclusions During the perinatal period, the labor management and nursing intervention of the parturient women with labor analgesia are significantly reduced, the cesarean section rate is significantly reduced, and the complication rate is reduced.

The paper collects patents relevant to hypolipidemic drugs in recent decade based on the Derwent database,reveals the development process of drug research in this field by taking advantage of the patent analysis method,and discusses the change of application quantity for patents over the years,distribution in the hotspot technology field and the main application organizations and their R&D orientations.

The morbidity and nortality of esophageal carcinoma showed an upward trend in recent years.Neoadjuvant radiochemotherapy is the nain mode of treatment for advanced esophageal carcinoma,including neoadjuvant chemotherapy,neoadjuvant radiotherapy and neoadjuvant radiochemotherapy.However,there are still many controversies on efficacies and advantages or disadvantages of treatment mode.In this article,authors will investigate the present situations and future direction of neoadjuvant therapy for locally advanced esophageal carcinoma.

The application and development of catalytic fluorimetry in recent years were reviewed with 103 references. The new technique and application of catalytic fluorimetric methods, such as the determination of catalyst and activator, multicomponent determination, enzyme catalysis, the effect of micelle in multicomponent determination and enzyme catalysis, the combination with flow-injection or stopped-flow tachniques and laser induced fluorimetry, time resolved fluorimetry, bioreactor control, biosensor, medicine assay, bioanalysis were mainly discussed. Future study was envisioned