1.IgG4-related sclerosing cholangitis: liver biopsy findings.
The Korean Journal of Hepatology 2011;17(1):76-79
No abstract available.
Aged
;
Biopsy, Fine-Needle
;
Cholangitis, Sclerosing/*pathology/ultrasonography
;
Humans
;
Immunoglobulin G/*blood
;
Liver/*pathology
;
Male
;
Tomography, X-Ray Computed
2.The application of high-throughput proteomics in cytopathology
Journal of Pathology and Translational Medicine 2022;56(6):309-318
High-throughput genomics and transcriptomics are often applied in routine pathology practice to facilitate cancer diagnosis, assess prognosis, and predict response to therapy. However, the proteins rather than nucleic acids are the functional molecules defining the cellular phenotype in health and disease, whereas genomic profiling cannot evaluate processes such as the RNA splicing or posttranslational modifications and gene expression does not necessarily correlate with protein expression. Proteomic applications have recently advanced, overcoming the issue of low depth, inconsistency, and suboptimal accuracy, also enabling the use of minimal patient-derived specimens. This review aims to present the recent evidence regarding the use of high-throughput proteomics in both exfoliative and fine-needle aspiration cytology. Most studies used mass spectrometry, as this is associated with high depth, sensitivity, and specificity, and aimed to complement the traditional cytomorphologic diagnosis, in addition to identify novel cancer biomarkers. Examples of diagnostic dilemmas subjected to proteomic analysis included the evaluation of indeterminate thyroid nodules or prediction of lymph node metastasis from thyroid cancer, also the differentiation between benign and malignant serous effusions, pancreatic cancer from autoimmune pancreatitis, non-neoplastic from malignant biliary strictures, and benign from malignant salivary gland tumors. A few cancer biomarkers—related to diverse cancers involving the breast, thyroid, bladder, lung, serous cavities, salivary glands, and bone marrow—were also discovered. Notably, residual liquid-based cytology samples were suitable for satisfactory and reproducible proteomic analysis. Proteomics could become another routine pathology platform in the near future, potentially by using validated multi-omics protocols.
3.The proteomic landscape shows oncologic relevance in cystitis glandularis
Jun Yong KIM ; Dohyun HAN ; Hyeyoon KIM ; Minsun JUNG ; Han Suk RYU
Journal of Pathology and Translational Medicine 2023;57(1):67-74
Background:
The relationship between cystitis glandularis (CG) and bladder malignancy remains unclear.
Methods:
We identified the oncologic significance of CG at the molecular level using liquid chromatography-tandem mass spectrometry-based proteomic analysis of 10 CG, 12 urothelial carcinoma (UC), and nine normal urothelium (NU) specimens. Differentially expressed proteins (DEPs) were identified based on an analysis of variance false discovery rate < 0.05, and their functional enrichment was analyzed using a network model, Gene Set Enrichment Analysis, and Gene Ontology annotation.
Results:
We identified 9,890 proteins across all samples and 1,139 DEPs among the three entities. A substantial number of DEPs overlapped in CG/NU, distinct from UC. Interestingly, we found that a subset of DEP clusters (n = 53, 5%) was differentially expressed in NU but similarly between CG and UC. This “UC-like signature” was enriched for reactive oxygen species (ROS) and energy metabolism, growth and DNA repair, transport, motility, epithelial-mesenchymal transition, and cell survival. Using the top 10 shortlisted DEPs, including SOD2, PRKCD, CYCS, and HCLS1, we identified functional elements related to ROS metabolism, development, and transport using network analysis. The abundance of these four molecules in UC/CG than in NU was consistent with the oncologic functions in CG.
Conclusions
Using a proteomic approach, we identified a predominantly non-neoplastic landscape of CG, which was closer to NU than to UC. We also confirmed a small subset of common DEPs in UC and CG, suggesting that altered ROS metabolism might imply potential cancerous risks in CG.
4.A Case of Primary Choriocarcinoma of The Fallopian Tube.
Yoon Seok KIM ; Young Han PARK ; Sung Suk SEO ; Jung Pil LEE ; Ki Hong JANG ; Hee Jae JOO ; Hee Suk RYU ; Kie Suk OH
Korean Journal of Gynecologic Oncology and Colposcopy 2001;12(1):52-57
Choriocarcinoma is a relatively rare malignancy of which characteristic is rapid metastasis to the other organs. It is related to the previous gestation or originated from the teratoma. Choriocarcinoma is mostly originated from the intrauterine chorionic villi, but it is rarely originated from the utreine cervix, fallopian tube, ovary, vagina and pelvic cavity. Primary choriocarcinoma of the fallopian tube is exceedingly rare and it is originated from ectopic tubal pregnancy, tubal migration from the intrauterine pregnancy or intratubal teratoma. 9 Symptoms and signs of the choriocarcinoma originated from the ectopic pregnancy are abdominal pain, vaginal bleeding, palpable adnexal mass, positive pregnancy test and amenorrhea. Thus it is difficult to distinguish choriocarcinoma from ectopic pregnancy on the basis of symptoms before the microscopic diagnosis presented.20 Effective treatment of choriocarcinoma is chemotherapy. Additional operation is possible. B-HCG is a useful measure for the follow up. We experienced a 36-year-old multigravida Korean woman who was diagnosed as the rupture of ectopic pregnancy after left salpingectomy in our hospital and then confirmed primary choriocarcinoma of the fallopian tube without metastasis on microscopic finding. Postoperative chemotherapy was performed with methotrexate. The follow up of disease is still on going at two month intervals and she has remained healthy, We report this case with review of literatures.
Abdominal Pain
;
Adult
;
Amenorrhea
;
Cervix Uteri
;
Choriocarcinoma*
;
Chorionic Villi
;
Diagnosis
;
Drug Therapy
;
Fallopian Tubes*
;
Female
;
Follow-Up Studies
;
Humans
;
Methotrexate
;
Neoplasm Metastasis
;
Ovary
;
Pregnancy
;
Pregnancy Tests
;
Pregnancy, Ectopic
;
Pregnancy, Tubal
;
Rupture
;
Salpingectomy
;
Teratoma
;
Uterine Hemorrhage
;
Vagina
5.CT and MR Findings of Brain Abscess: Focus on Thickeness and Signal Intensity of Abscess Wall.
Tae Il HAN ; Yup YOON ; Kyung Nam RYU ; Woo Suk CHOI ; Mi Jin SONG ; Joo Hyung OH
Journal of the Korean Radiological Society 1994;30(6):993-997
PURPOSE: The purpose of this study is to evaluate the CT and MR findings of brain absecss with emphasis on the thickeness and signal intensity of abscess wall. MATERIALS AND METHODS: Twenty CT scans and seven MR studies of 23 patients with brain abscess (27 abscesses) were retrospectively reviewed with respect to thickeness and signal intensity of abscess wall, shape and size of abscess, etc. Thickness of abscess wall was measured in the greatest abscess diameter on the CT scans and MR images obtaihed after intravenous administration of contrast material. RESULTS: Enhancing abscess wall was 2mm--6mm (average 3mm) thick. The abscess wall was uniform in thickness in 14 cases (52%), thinner in the roedial wall than lateral wall in 8 cases (30%), and thicker in the medial wall than lateral wall in 5 cases (18%). Signal intensity of abscess wall was isointense relative to gray matter on Tl-weighted MR images and hypointense on both proton- and T2-weighted MR images in 7 out of 8 lesione (88%). The inner margin of the abscess wall was smooth in 14 (52%) and irregular in 13 lesions (48%). The outer margin was smooth in 15 (55%) and irregular in 12 lesions (45%). The size of the abscesses was variable, ranging from lcm to 6cm in diameter. They were round (16 cases), elliptical (6 cases), or multilobulated (5 cases) in shape. Satellite or daughter abscesses were found in 5 patients. CONCLUSION: Abscess wall showed variable thickness in the medial and lateral walls with no specific findings. Other CT and MR findings were also nonspecific, although hypointensity of abscess wall on T2-weighted MR images may be helpful in diagnosis of brain abscess.
Abscess*
;
Administration, Intravenous
;
Brain Abscess*
;
Brain*
;
Diagnosis
;
Humans
;
Nuclear Family
;
Retrospective Studies
;
Tomography, X-Ray Computed
6.A Case of Endometrial Cancer with Polycystic Ovary Syndrome.
Sang Tae AHN ; Young Han PARK ; Hee Sug RYU ; Hee Jae JOO ; Jung Pil LEE ; Kie Suk OH
Korean Journal of Gynecologic Oncology and Colposcopy 2000;11(4):416-421
Polycystic ovary syndrome is characterized by symptoms of oligomenorrhea, amenorrhea, infertility, hirsutism and obesity. It is known that the women with such diseases would have higher risks to coronary heart diseases, hypertension, DM and endometrial cancer later on, Known risk factors of endometrial cancer are nullipara, late menopause, obesity, DM, unopposed estrogen, tamoxifen treatment, and atypical endometrial hyperplasia ect. 75% of endometrial cancer occurs in age over 50 and 4% of endometrial cancer occurs in age under 40. Particulary endometrial cancer concurrent polycystic ovary syndrome occurs usually in people under age 40 and is commonly well differentiated cell type and is related to good prognosis. We experienced a case of endometrial cancer with polycystic ovary syndrome, who was early dignosed, presented with infertility and got an operation in our hospital, and report this case with a brief review of literature.
Amenorrhea
;
Coronary Disease
;
Endometrial Hyperplasia
;
Endometrial Neoplasms*
;
Estrogens
;
Female
;
Hirsutism
;
Humans
;
Hypertension
;
Infertility
;
Menopause
;
Obesity
;
Oligomenorrhea
;
Polycystic Ovary Syndrome*
;
Prognosis
;
Risk Factors
;
Tamoxifen
7.The Association of Plasma HDL-Cholesterol Level with Cardiovascular Disease Related Factors in Korean Type 2 Diabetic Patients.
Hye Sook HONG ; Jong Suk PARK ; Han Kyoung RYU ; Wha Young KIM
Korean Diabetes Journal 2008;32(3):215-223
BACKGROUND: Cardiovascular disease (CVD) is the major cause of death in type 2 diabetic patients. The purpose of this study was to investigate the characteristics of Korean type 2 diabetes mellitus (DM) patients according to plasma high density lipoprotein (HDL) cholesterol level and to document the effect of diet on HDL-cholesterol. METHODS: The subjects were 252 (male: 134, female: 118) Korean type 2 DM patients recruited from a general hospital's DM clinic and divided into low HDL-cholesterol group (male < 40 mg/dL, female < 50 mg/dL) and control group (male > or = 40 mg/dL, female > or = 50 mg/dL). Anthropometric and hematological variables and dietary intake were assessed by the groups. RESULTS: The subject's mean age was 60.2 +/- 1.1 years and duration of diabetes was 9.5 +/- 1.0 years. Anthropometric measurements (body fat mass, % body fat, WHR, fat free mass, and muscle mass) and BMI were not significantly different between two groups. The male subjects with low HDL-cholesterolemia showed higher Atherogenic Index (AI, P < 0.001) and higher % carbohydrate from energy than control group (P < 0.01). The female subjects with low HDL-cholesterolemia showed higher AI (P < 0.001) and a tendency of higher triglyceride level and lower intake of energy, protein, lipid, vitamin B1 and vitamin E (P < 0.05) than control group. CONCLUSION: The subject with low HDL-cholesterolemia showed significantly higher AI. Male subject with low HDL-cholesterolemia consumed higher carbohydrate and female subject with low HDL-cholesterolemia showed lower intakes of many nutrients. This result suggests the importance of an adequate and balanced diet to manage type 2 DM patients to prevent CVD complications.
Adipose Tissue
;
Cardiovascular Diseases
;
Cause of Death
;
Cholesterol
;
Diabetes Mellitus, Type 2
;
Diet
;
Female
;
Humans
;
Lipoproteins
;
Male
;
Muscles
;
Plasma
;
Thiamine
;
Vitamin E
;
Vitamins
8.A Clinical - Pathological Study of Uterine Smooth Muscle Tumor of Uncertain Malignant Potential.
Hee Jong LEE ; Hee Sug RYU ; Young Han PARK ; Hee Jae JOO ; Seung Chun YANG ; Kie Suk OH
Korean Journal of Obstetrics and Gynecology 2000;43(10):1769-1774
No abstract available.
Muscle, Smooth*
;
Smooth Muscle Tumor*
9.Postoperative adjuvant CCRT concurrentchemoradiotherapy for selected early stage cervical cancer patients following radical hysterectomy and pelvic lymph node dissection.
Young Ah KIM ; Hee Sug RYU ; Mi Son CHUN ; Ki Hong CHANG ; Young Han PARK ; Kie Suk OH
Korean Journal of Obstetrics and Gynecology 2000;43(10):1725-1730
No abstract available.
Humans
;
Hysterectomy*
;
Lymph Node Excision*
;
Lymph Nodes*
;
Uterine Cervical Neoplasms*
10.Stewart-Treves Syndrome Arising in a Chronic Lymphedematous Leg.
Han Won RYU ; Kyu Suk LEE ; Jae We CHO
Korean Journal of Dermatology 2011;49(1):32-35
Angiosarcoma is a rare malignancy of a vasculo-eptihelial origin. It is divided into three categories. The first category is primary anigosarcoma, which occurs in the face and scalp of elderly patients without any predisposing factors. The second is Stewart-Treves Syndrome, which is a subset of chronic lymphedema. The third is a post irradiation angiosarcoma that occurs at the site of prior irradiation. Generally in Stewart-Treves Syndrome, there is a pre-existing chronic lymphedema on the upper extremities due to the wide excision of breast cancer with or without radiation therapy. We here report on a case of a 60-year-old female with chronic lymphedema on the leg and who had a history of hysterectomy due to cervical cancer. The patient had purple-colored tender nodule and ulcer with exudate on the chronic lymphedematous leg and was diagnosed as angiosarcoma by a skin biopsy.
Aged
;
Biopsy
;
Breast Neoplasms
;
Exudates and Transudates
;
Female
;
Hemangiosarcoma
;
Humans
;
Hysterectomy
;
Leg
;
Lymphedema
;
Middle Aged
;
Scalp
;
Skin
;
Ulcer
;
Upper Extremity
;
Uterine Cervical Neoplasms