The distribution of exogenous C(14)-succinic acid by Clonorchis sinensis incubated in Tyrode medium was studied by using autoradiographic techniques. A comparison was made between macro and microautoradiographs of this worm showed that black grains derived from labeled substance were distinctly observed in parenchymal tissue, oral sucker, ventral sucker, ovary, testes, and uterine tubules with eggs. In pharynx and intestine, a low density was monitored while negligible activity was found in seminal receptacle and vitelline follicles. In studying the further details, microautoradiography was utilized. The most radioactive regions were the reticular tissue and subcuticular musculature of this fluke, closely compatible with the finding of macroautoradiography. Apparent black grains of radioactivity were also found in oral sucker, ventral sucker, pharynx, uterine tubules, eggs in uterus, and intestinal ceca. Structures showing the least radioactivity included ovary, seminal receptacle and vitelline folliciles.
parasitology-helminth-trematoda ; Clonorchis sinensis ; Tyrode ; autoradiography ; biochemistry-succinic acid ; Tyrode ; succinic acid

122 cases of the genitourinary tract tumors admitted to the Department of Urology, National Medical Center, during the period, Aug. 1968 to July 1975 and the followings are the results. 1. 122-cases(12.8%) were tumor patient among 954 (male 702, female 252) Total admitted patients during 7 years. 2. Age distribution was from 5 months to 83 years showing the highest incidence in the 7th decade (30.3%) and cases over 41 years of age were 99(81.1%) 3. Sex ratio of male and female was 5.4:1(103:19) 4. In 122 cases, 42 cases{34.4%) were benign, 77 cases(63. 1%) were malignant and remainders were uncertain pathologically. 5. Among 122 cases, 57 cases(46.7%) were vesical, 35 cases(28.7%) prostatic, 19 cases(15.6%) renal, 5 cases(4.1%) penile. 3 cases(2.5%) testicular and 3 cases(2.5%) were urethral tumors. 6. Operations were performed in 114 patients (93.5%).
Age Distribution ; Female ; Humans ; Incidence ; Male ; Sex Ratio ; Urology

A study was made on 1,302 cases of skin tumors which were obtained mainly from surgical pathology specimen during the period of 14 years from 1973 to 1986 at Department of Dermatology in Korea University Hospital. The results were summarized as follows : 1. Of the 1,302 cases of skin tumors, 1,143 cases(87.8%) were benigh tumor, 159 cases(12.2%) of malignant tumor and male to female ratio was 1.1: l. 2. Among the 1,143 cases of benign tumors, 301 cases of epidermal cyst, 183 cases of lipoma, 173 cases of pigmented nevi and 88 cases of hemangioma were noted. 3. Among the 159 cases of malignant tumors, 46 cases of squamous cell carcinoma, 27 cases of basal cell carcinoma, 26 cases of malignant melanoma, and 17 cases of metastatic carcinoma were observed. 4. The sites of predilection of the epidermal cysts were trunk (21.9%), face (20.9%), and neck (13.9%) and lipoma was trunk (48.6%). The sites of predilection of the suamous cell carcinomas were penis (11.1%), sclap (12.9%), face (18.5%), and leg (11.1%), basal cell carcinoma was face (55.6%) and malignant melanoma was sole (26.9%). 5. Among the 17 cases of cutaneous metastatic cancer, primary sites were breast (4 cases), pancreas (1 cases), lung (2 cases), stomach (1 cases) and unkown sites (9 cases).
Breast ; Carcinoma, Basal Cell ; Carcinoma, Squamous Cell ; Dermatology ; Epidermal Cyst ; Female ; Hemangioma ; Humans ; Korea ; Leg ; Lipoma ; Lung ; Male ; Melanoma ; Neck ; Nevus, Pigmented ; Pancreas ; Pathology, Surgical ; Penis ; Skin* ; Stomach

No abstract available.

PURPOSE: To examine the expression of Connective Tissue Growth Factor (CTGF) in osteosarcoma and to evaluate its role in osteosarcoma invasion and proliferation. MATERIALS AND METHODS: The mRNA expression of CTGF from 23 patient-derived osteosarcoma cell lines was examined, and the role of CTGF in cell invasion and proliferation was examined using siRNA transfection. RESULTS: The over-expression of CTGF mRNA was observed in 17 cell lines (74%). CTGF-specific siRNA transfection into SaOS-2 and MG63 cell lines resulted in efficient knockdown of CTGF expression on Western blot analysis. siRNA transfected cells showed decreased migration on Matrigel invasion assay and decreased cell proliferation on WST-1 assay. CONCLUSION: These results indicated that the CTGF expression may play an important role in osteosarcoma progression, and may be a therapeutic target of osteosarcoma.
Blotting, Western ; Cell Line ; Cell Proliferation ; Connective Tissue Growth Factor ; Osteosarcoma* ; RNA, Messenger ; RNA, Small Interfering ; Transfection

No abstract available.
Polymethyl Methacrylate*

The main objectives of this study were to know the prevalence of cerebrovascular disease, to find the important risk factors of cerebrovascular diseases. This study is a part of Eumseong Community Health Project supportes by GTZ(West German Government). 116 perceived cases of cerebrovascular disease were first screened by health interview and examinations and 80 cases were diagnosed as a cerebrovascular group. For comparison, 80 cases were matched with their neighbor controls of the same sex and the similar age. 1. The prevalence rate for cerebrovascular diseases was 476.3 per 100,000 population which is considered to be very high compared with that of other countries. Age adjusted rate for cerebrovascular diseases was 261.6 per 100,000. 2. Among the risk factors examined for the association with cerebrovascular diseases by case-control study, and analyzed by paired marginal test(McNemar's chi-square-test) and odds ratio, only hypertension showed high significant statistical association.
Case-Control Studies* ; Hypertension ; Odds Ratio ; Prevalence ; Risk Factors* ; Rural Population*

No abstract available.
Hemorrhage* ; Hemorrhoids* ; Ligation* ; Rubber*

No abstract available.
Adsorption* ; Agglutination* ; Animals ; Caragana* ; Erythrocytes* ; Humans* ; Mice*

The prognosis of transitional cell carcinoma(TCC) of the urinary bladder is related to histopathologic parameters, among which the clinical stage and histopathologic grade are most important prognostic determiantors. Recently the immunohistochemical assessment of proliferating cell nuclear antigen(PCNA) and nucleolar organizer region number(AgNORs) can obtain the PCNA, and AgNORs stainings were studied in 55 the sequential biopsies of 22 recurrent TCCs of the urinary bladder. 6 cases showed the increased changes of grade, of which 5 cases was independently to the change of grade. The AgNORs in 18 cases showed increase in 10 cases. The comparison between PCNA count and AgNORs score according to grade was performed in the changes between grade II and III, both PCNA count and AgNORs score were increased with in crease of grade. However, The change of the PCNA count was stastically significant, but not AgNORs score.
Biopsy