Vascular endothelial growth factor (VEGF)-specific effects on vascular endothelial cells,by promoting endothelial cell proliferation,increased vascular permeability,to induce tumor angiogenesis and lymphangiogenesis in tumor development has played key role.Studies have shown,VEGF and its receptor expression and the incidence of thyroid cancer,lymph node metastasis,prognosis,inhibition of VEGF and its receptor expression in anaplastic thyroid cancer and medullary cancer treatment in a certain application.

In clinical nursing practice, ethnics dilemmas which nurses facing with are not usually a simple question of right and wrong or yes and no, but two or more mutual conflicts under some special circumstances; In order to help nurses to understand and deal with ethnics dilemmas effectively and reasonably, the authors summarize the four different ethnics dilemmas: the different ethical principles selection dilemma, the different stakeholder selection dilemma, the different interpersonal ethnics dilemmas and the conflict of the nursing professional ethics and the role morality. Typical cases of common ethnic dilemmas were summarized and analyzed in this paper.

Sixty-three cases of neurodermatitis were treated with seven-star needle, tapping the affected area until slight bleeding and then ignite moxa-stick to make lsft-right repeated rotation moxibustion about 2 cm away from the skin. The total effective rate was 100% after three treatment course.

Ovjective To observe the surgically excised specimens from eyes with hemorrhagic agerelated macular degeneration (AMD).Methods Thirty-six surgically excised specimens were captured from 36 patients with hemorrhagic AMD,26 specimens were diagnosed as occult choroidal neovascular membrane (CNVM),10 specimens were diagnosed as polypoidal choroidal vasculopathy (PCV).All specimens were routinely processed by hematoxylin and eosin,periodic acid-Schiff's stain and Masson stainings.At the maximum horizontal and vertical slice of the specimens,the category and amount of the cells in the specimen were recorded,as well as the relationship between the specimens and the surrounding tissue.Results The 36 specimens are categorized as neovascular membrane dominant (19/36),collagen fiber dominant (6/36),blood clot dominant (8/36) and degenerated thickened Bruch's membrane dominant (3/36).Eighteen occult CNVM specimens and 1 PCV specimen are categorized as neovascular membrane dominant; all 6 collagen fiber dominant specimens are occult CNVM; 1 occult CNVM and 7 PCV specimens are categorized as blood clot dominant; and 1 occult CNVM and 2 PCV specimens are categorized as degenerated thickened Bruch's membrane dominant.The occult CNVM categorized as neovascular membrane dominant present as small blood vessel with single endothelium cell attached; the PCV specimen categorized as neovascular dominant presents as big blood vessel with thick vessel wall under the Bruch's membrane,retinal pigment epithelium and choroidal melanocyte are both observed in the PCV specimens.Conclusion The components of the specimens captured from eyes with hemorrhagic AMD are diversified.

Four alkaloids, berbamine, jatrorrhizine, palmatine, berberine present in different parts of sankezhen, a berberis plant in Gansu Province, were determined by HPLC

OBJECTIVE: Tension affects the functions in physiology, psychology and immunity. The immune reaction induced may affect other physiological functions and psychological feelings, resulting in reaction in physiology and/or behavior. The present state in the studies on organic molecular changes during stress at home and broad was synthesized so as to explore the change of immune function in psychological stress and its significance.DATA SOURCE: Relevant literatures on early rehabilitation of cerebral apoplexy were looked up in Medline from January 1997 to December 2003.The retrieved words: "stress, immune, depression, neuropsychiatric disorder", limited in English.STUDY SELECTION: By first examination of data, the literatures with stress group and the control involved were selected, and then researches on randomized clinical experimental study and laboratory experiment were included. Excluded criteria: the researches without randomized clinical experiment, repeated research and synthetic literature were excluded.DATA EXTRACTION: Totally, 43 papers on randomized-controlled clinical experimental research and laboratory experiment on organic molecular changes in stress were collected and 25 papers of them were employed. Of 18 excluded papers, 7 papers referred to therepeated research and 11 papers are Meta analyzing study. Totally 25 experiments have evaluated the change of immunocytes, change of cytokines and their receptors and neuroendocrinal factors in change of immune function during psychological stress.DATA SYNTHESIS:DATA SYNTHESIS: The molecule mechanism of stress on physiological,psychological and immunological functions is based on feedback regulation of multiple direct information net, neuroendocrinal cell, immunoctye and immunological medium. ① Change of immunocyte: Stress is associated with inhibition of neurokinin cell activity, reduced proliferation and inhibition of lymphocyte to mitogen and to IL-2 and IFN-γ respectively. Senile depression is related with the increased subgroup of neurokinin-T cell. Levels of monocyte reactive oxygen species are remarkably correlative with decreased natural killer cell activity in patient with depressive disorder. ② Change of cytokines and their receptors: Psychological stress and neuropsychiatric disorder threaten the safety of immune function, the mechanism of which is probably related to the activity of cytokines in immune system and other regulating factors of inflammation. ③ Neuroendocrinal factors in change of immune function: Cytokine transports actively to central nerve system and is also released from neurogial cell to activate neurogial cell in central nerve system to produce other cytokines. The chain between stress and disease is as follows: tension-stress response-alternation of neuroendocrine-imbalance of immune response-incidence of disease.CONCLUSION: The mechanism of the effects of psychological stress on immune function is very complicated, involving the coordination of central neuroendocrinal system and immune system. Only the relationship between the alternations of neuroendocrinology and immunology in psychology with health and disease is searched more extensively, does the understanding be enhanced on the interconnection of stress, depression, health and disease and the defensive capacity intensified.

Low back pain has a serious impact on people's lives in t he world.With the approaching of aging society in China,the number of low back pain increased rapidly.Though many of the pathological factors in spine might lead to low back pain,Modic changes (MCs) was one of the most important factors.Therefore,to get familiar with MCs in the etiology and pathology has a positive practical significance.Although a large number of studies have analyzed the epidemiological and imaging characteristics of MCs,there are relatively few studies on the histological and pathological fields of MCs.In recent years,researchers have gradually shed some light on etiology of MCs and found that low toxic bacterial infection and autoimmune reaction may play a vital role in the development of MCs.The damage of vertebral body or endplate is closely related to the etiopathogenesis of MCs.In addition,to reveal the pathological characteristic of MCs is also a good attempt for better understanding of MCs.There are various imaging changes in MCs,however,what is the pathological basis behind it.It is also unclear that why different types of MCs are capable of transformation? When MCs occur,the activation of some cytokines (such as IL-6,RANKL,etc.) and certain receptors (TLR,PPARγ,etc.) can interfere with the formation and metabolism of the nearby bone marrow cells,break the balance of formation and absorption in trabecular beam and further change their microstructures through various pathways.During this dynamic balance process,the different types of MCs can be transformed into each other.The specific type may not only depend on the intensity and duration of inflammatory stimulus,but also rely on the composition and metabolic status of the bone marrow.Although the study of animal models has made some progress,some limitations still exist in practical course.Due to the unclear mechanism of MCs,the study on etiology and pathology of MCs may play a great important role in clarifying this issue.

Objective To reveal and compare the immunogenicity of glycoprotein from nucleus pulposus and collagen from anulus fibrosus in rabbit intervertebral disc. Methods According to the similarity principle of genome biology and the amount of nucleus pulposus in different intervertebral disc, Glycoprotein from nucleus pulposus was harvested from the intervertebral disc of 20 rabbits and placed into the psoas by anterolateral approach. Similar implantation of collagen from anulus fibrosus was performed in another 20 rabbits. The implantations of autologous muscle in rabbits serve as a control group.After one week,two weeks, four weeks, six weeks and eight weeks, the rabbits were killed, the tissue was harvested. HE staining was applied to detect the degree of vascularization and infiltration of lymphocytes. Immunohistochemistry were used for analysis of T-helper cells (CD4+) and cytotoxic T cells (CD8+) in the tissue. Results HE staining showed the infiltration of lymphocytes was found just one week after implantation and vascularization was seen at the 2nd week in the glycoprotein implanted group. However in collagen implant group,small amounts of lymphocytic and atypical vascularization were seen at the 4th week. Immunohistochemistry revealed that the proportion of activated T cells (CD4+ and CD8+) was significantly higher in the glycoproteinimplanted group at all time points, as compared with the collagen-implanted group and sham group. The proportion of activated T cells was higher in collagen-implated group than that of in sham group . Conclusion The glycoprotein in nucleus pulposus is easier to induce the differentiation and mature of T cell. The antigenicity of glycoprotein in nucleus pulposus was stronger than that of collagen in anulus fibrosus in rabbit intervertebral discs.