Caroli's disease is a rare autosomal recessive hereditary disease. The low mobidity, insufficient awareness of doctor and complex clinical manifestations of the disease are likely to result in misdiagnosis and missed diagnosis.The pathogen, typing, pathology, clinical manifesation and therapy of this disease are discussed to recognise the disease better.

Ischemic preadaptation and ischemic postconditioning are the self-protection mechanism of the body, can reduce blood anoxic injury of brain tissue, inhibit nerve cell apoptosis, and reduce infarction area.However, due to the time of occurrence of ischemic disease is unpredictable, ischemic postconditioning research to guide clinical work more operational and practical, and thus become the focus of research. Apoptosis of programmed cell death is widespread in the process of biological life and has a close relationship with jak2/stat3/bcl-2 signaling pathway, PI3K/AKT signaling pathways and apoptotic molecules.microRNA as a class of highly conserved non-coding small RNA, its transcriptional expression involved in regulating cell value, development and apoptosis in a series of life activities.

Objective:To explore the route of automatic computer-aided design for occlusal rest of the removable partial denture.Methods: A new module for the design of occlusal rest was developed.The three-dimensional data of a partially dentate dental cast was obtained and imported into the new module.The contour of the occlusal's tissue surface was identified.Then,the tissue surface and polished surface were constructed respectively and connected to each other to form an integrated entity.Results: The surface of the occlusal rest was smooth and the curvature matched that of the surrounding area of the abutment tooth.The accuracy was satisfying.Conclusion: Design of occlusal rest was much more convenient and time-saving using the newly developed module.The future development of special software package for the design of removable partial denture will benefit from it.

Objective To analyze the risk factors of Gram‐negative bacterial blood‐stream infection in ICU for conducting the risk evaluation and guiding medication .Methods The inpatients were diagnosed with Gram‐negative bacterial blood‐stream infec‐tion in ICU of the Renmin Hospital of Wuhan University from January 2013 to December 2014 were retrospectively surveyed and analyzed .The risk factors of blood‐stream infection caused by Gram‐negative bacteria were analyzed and screened by the Logistic re‐gression analysis method .Results A total of 172 case‐times of blood‐stream infection occurred in ICU during this period ,including 93 case‐times of Gram‐negative bacterial infection .The Gram‐negative pathogenic bacteria were Acinetobacter baumanii ,Klebsiella pneumoniae ,Acinetobacter calcoaceticus baumanii ,E .coli ,pseudomonas aeruginosa ,etc .Except E .coli was mainly originated from community acquired infection ,other bacteria were mainly originated from nosocomial infection .In order to differing from other pathogenic bacterial blood stream infection ,the Logistic regression analysis results showed that the independent risk factors of G‐negative bacterial blood‐stream infection in ICU had serum PCT levels over 10 .0 ng/mL (OR= 60 .52 ,P= 0 .001) ,receiving the therapy of carbapenem and third generation cephalosporins (OR=16 .09 ,P=0 .03) ,hospitalization duration less than 2 weeks be‐fore suffering from disease (OR=13 .79 ,P=0 .03) and digestive system basic disease(OR=12 .94 ,P=0 .01) .Conclusion Gram‐negative bacterial blood‐stream infection in ICU of the Renmin Hospital of Wuhan University is mainly caused by multi‐drug resist‐ant bacteria .Serum PCT level over 10 .0 ng/mL ,hospitalization duration less than 2 weeks before infection ,receiving the therapy of carbapenem and third generation cephalosporin and basic diseases of digestive system are the independent risk factors influencing the occurrence and diagnosis of blood‐stream infection .

OBJECTIVE:To optimize the extracting process of the total flavonoids from Herba Epimedii.METHODS: The extract technology of the total flavonoids from Herba Epimedii was optimized by orthogonal experiment with the yield of total flavonoids from Herba Epimedii as index and with the extraction temperature,extraction time,the ratio of solid to liquid and the concentration of ethanol as factors.RESULTS:The optimum extracting conditions for the total flavonoids from Herba Epimedii were as follows:the reflux extraction was conducted twice(1 hour/time) at 80 ℃ with 60% ethanol as solvent,and the ratio of solid to solution was 1∶10.CONCLUSION:The optimum extracting technology is simple and reasonable,and it serves as a good reference for the extracting process of the total flavonoids from Herba Epimedii.

Objective:To retrospectively analyze the CT features among gastric cancer, gastric lymphoma and gastric malignant stromal tumors, evaluated the application value of MDCT in gastric malignant tumor.Methods: Sixty two patients with gastric malignancies tumor confirmed by pathology underwent MDCT plain scanning and triphasic contrasted scanning before operation. Referring to the pathological results, to observe the CT signs, include: scope of tumors and involvement, the density of tumors and enhanced characteristics, mucosal membrane, the change of the stomach softness, peripheral lymph node enlargement, the involvement of other organs, and to make statistical analysis for imaging features of these tumors byx2 testing.Results: Sixty two cases of gastric malignant tumor included 42 cases of gastric carcinoma, 12 cases of gastric lymphoma and 8 cases of gastric malignant stromal tumor, were confirmed by postoperative pathology. The CT manifestations were as follows: the stomach mucous membrane surface involvement in all the gastric cancer, the positive rate was significantly higher than gastric lymphoma and stromal tumor. The area involved by lymphoma were more than two partitions, significantly higher than that of gastric cancer and stromal tumor (x2=36.643,x2=20.00;P<0.05). 39 cases (92.8%) of gastric cancer and 8 cases (100%) of malignant stromal tumors were significantly enhanced, but all gastric lymphoma were not obvious enhancement. The positive rate of enlarged lymph nodes in gastric cancer(61.9%) and lymphoma(83.3%) were higher than stromal tumor(12.5%)(x2=9.731,x2=6.603;P<0.05).Conclusion: The CT manifestations of the gastric cancer, lymphoma, malignant stromal tumors had different characteristics. Multislice CT for the diagnosis and differential diagnosis of gastric malignant tumor has important value.

BACKGROUND:Bone marrow mesenchymal stem cels can produce a variety of cytokines to provide some support for the growth and proliferation of myeloma cels.
OBJECTIVE:To investigate the effects of astragalus polysaccharides on the proliferation of bone marrow mesenchymal stem cels from patients with multiple myeloma and cytokine secretion.
METHODS:Bone marrow mesenchymal stem cels were isolated by density gradient centrifugation from the bone marrow of a healthy volunteer and a multiple myeloma patient, and were cultured and identified. Passage 3 bone marrow mesenchymal stem cels were colected, and the growth curve was depicted by the enzyme marker method. MTT method and flow cytometry were used to detect the effects of different concentrations (0.5, 1, 2, 4, 6, 8 g/L) of astragalus polysaccharides on the proliferation and cel cycle of bone marrow mesenchymal stem cels. The expression levels of interleukin-1β and interleukin-6 were detected by ELISA method.
RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cels from both the healthy control and multiple myeloma patient showed negative expression of CD34 and positive expression of CD44. The obtained cels from two sources had the characteristics of bone marrow mesenchymal stem cels. Cels from the healthy control had significantly shorter doubling time than those from the myeloma patient. After treated with 1 and 2 g/L astragalus polysaccharide, cel proliferation rate in the multiple myeloma group was significantly higher than that in the healthy control group; after treated with 6 and 8 g/L astragalus polysaccharide, the cel proliferation rate was significantly lower in the multiple myeloma group than in the healthy control group. After treated with 1 g/L astragalus polysaccharide, compared with the healthy control, the proportion of bone marrow mesenchymal stem cels from the multiple myeloma patient in G0/G1 phase was significantly decreased, while the proportions of cels in both S phase and G2/M phase from the myeloma patient were significantly increased. Interleukin-1β and interleukin-6 levels of bone marrow mesenchymal stem cels from the patient with multiple myeloma were significantly higher than those of cels from healthy control. After intervention with 0.5, 1, 2 g/L astragalus polysaccharide, the interleukin-1β and interleukin-6 levels of bone marrow mesenchymal stem cels from the patient with multiple myeloma were al significantly decreased. In conclusion, a certain concentration of astragalus polysaccharides can promote the proliferation of bone marrow mesenchymal stem cels and decrease the levels of interleukin-6 and interleukin-β in patients with multiple myeloma.

Objective To observe the therapeutic effect of Ningxin Granule Heart-Quieting Granule for pediatric viral myocarditis.Methods Totally 60 cases of pediatric viral myocarditis were randomly divided into treatment group (n=30) and control group (n=30).There was no significant difference between two groups in sexuality,age and pathogenetic condition.Patients in treatment group took Ningxin Granule,while ATP,CoA,Vit C,Inosine and Ribavirin were given to control group by intravenous drip.Results The treatment group was superior to control group in relieving symptoms,reducing the bearing premature,CK-MB,cTnI and LVEDD,and increasing LVEF.Conclusion Ningxin Granule can obviously relieve symptoms and signs,reduce the bearing premature,decrease the level of myocardial enzyme and improve the cardiac function.

Objective To explore the level and main functions of P-type ATPase7B in hepatic cells of patients with hepatolenticular degeneration(HLD).Methods The hepatic cells from 5 normal controls and 9 patients with HLD were cultured in vitro. P-type ATPase7B levels in hepatic cells were examined and compared by SDS-PAGE and Western-blot techniques.Results Compared with the controls, 9 patients displayed various changes of electrophoresis strip. Almost normal strips at 155?103 were found in 3 cases, no strip was found in 1 case, and thinner and lighter strips were showed in the remain 5 cases. 6 cases presented abnormal specific reaction strips.Conclusion Mutations of gene ATPase7B in HLD patients cause change of P-type ATPase7B in quantity and quality, thus leads to dysmetabolism of copper.

180 U/L.CK-MB raised in only 5 cases(15.6%),and the highest was 28 U/L.Of the 218 MPP patients,only the 5 patients(2.3%) with raised CK-MB levels could be diagnosed as myocardial damage complicated with MPP.Conclusions The abnormal ECG is more important than myocardial zymogram in the diagnosis of myocardial damage complicated with MPP.Only higher level of certain myocardial zymogram without abnormal ECG can not be diagnosed as myocardial damage if lack of clinic and other objective evidences for myocardial damage.In watching out for myocardial damage,excessive examinations and treatments shall be avoided if possible.