Tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) is a new anti-tumor biological agents which is very hot in recent years through its death receptor-induced apoptosis of tumor cells,and non-toxic to normal cells,and has synergy with the chemotherapy drug,but there is also resistance mechanisms.This article will review the biological characteristics of TRAIL and its receptors,TRAIL-induced apoptosis of tumor cells,as well as the mechanism of TRAIL in leukemia in the progress of treatment.

Conjunctival Impression Cytology (CIC) method was modified to assess vitamin A level more exactly and simply in China. The infants (

Objective To evaluate the impact of integrated information management platform on life style of diabetic patients. Methods A total of 84 diabetic patients were assigned to the intervention group and the control group by random digits table method with 42 cases each. All patients were in the hospital and after discharge given conventional three-tier management model. The intervention group was given comprehensive information management platform, three-tier management model mediated on this basis. The life style, fasting blood glucose, glycated hemoglobin were collected and compared between the two groups before and after intervention. Results After 9 months of intervention, the dietary pattern of the intervention group was more in line with the Chinese Dietetic Association's recommended dietary standard, in which staple food decreased from (343.90 ± 225.61) g before intervention to (311.71 ± 144.46) g, and meat decreased from (61.52 ± 59.73) g before intervention to (50.07 ± 36.16) g, and the lipid content decreased from (32.67 ± 21.31) g before intervention to (28.57 ± 19.52) g, compared with the control group (376.49 ± 129.58), (73.90 ± 55.15), (45.78 ± 20.03) g, there were significant differences(t =2.076, 2.278, 3.841, all P<0.05). The number of physical exercises increased from 30 before intervention to 37 cases, the number of moderate exercise increased from 12 cases before intervention to 20 cases, and the median of exercise scores increased from 11.000 (26.500) points before intervention to 33.000 (23.500) points, compared with the control group 15.000(18.000) points, there was significant differences (Z =3.687, P<0.01). The fasting blood glucose, glycated hemoglobin were (7.73 ± 1.31) mmol/L, (6.75 ±1.81)% after intervention and 8.000(2.200) mmol/L, 7.600(1.600)%before intervention in the intervention group, compared with the control group (8.60 ± 2.25) mmol/L, (8.81 ± 2.63)%, there were significant differences(t =2.111, 4.062, P<0.05 or 0.01). Conclusions Integrated information management platform management mode three-mediated diabetes may urge to improve the existing way of life, better control of blood sugar.

Objective: To establish a peptide nucleic acid-mediated one-step PCR assay for detecting K-ras mutation, and to evaluate its diagnostic value. Methods: We developed a one-step polymerase chain reaction (PCR) approach with melting curve analysis using wild-type specific peptide nucleic acid (PNA) and fluorescent dye SYBR Green I to determine the genotypes in codon 12 and 13 of K-ras oncogene. The result of our method was compared with that of restriction fragment length polymorphism (RFLP) analysis. Results: Our method simultaneously examined condon 12 and 13 of K-ras oncogene, and was easy to perform. The sensitivity of our method was 0.001% when in a 105-fold excess of wild-type K-ras DNA. The detection limit was 102 copies. Our method could be used for large sample test, with the time of a single test being 1 hour. The limit of traditional method was 103 copies and the sensitivity was 0.01%. The testing time was about 2 days for samples which needed only 1 hour using our method. Conclusion: Our method has obvious advantage over RFLP analysis; it is suitable for detecting K-ras mutation in large samples and can be used as an effective method for early diagnosis of pancreatic cancer.

Objective To apply the membrane technology for purifying rough cordyceps polysaccharides solution,the optimal operation condition and effective cleaning method were researched.MethodsTo mainly analyze the flux of membrane and its recovery.Results The optimal experiment operation condition obtained is 60℃,pH value 7-8,and pressure 0.3-0.4 MPa,by which the polysaccharides recovery was about 30%,more than by traditional ethanol extraction,and the most impurities as infusibility substance and protein colloid etc.were taken out.Moreover,the flux of membrane recovery can be above 90% after 80℃ water and 1% NaOH solution was alternately used to wash the fouling membrane under intermittent little back-pulsing. Conclusion The process of membrane technology purifying rough cordyceps polysaccharides is simpl and feasible.

Objective To investigate the expressions of SOCS3 and IL‐6 in triple‐negative breast cancer(TNBC)and their clinical significance.Methods The expressions of SOCS3 and IL‐6 in TNBC and normal breast cells were detected by Western blot and fluorescence quantitative PCR.Immunohistochemical staining was used to detect their expressions in 84 TNBCs ,33 breast fibromas and 11 normal breast tissues. The correlation of their expressions in TNBC and the relationship between their expressions and clinical pathological parameters were analyzed as well.Results SOCS3 was not expressed or down‐expressed in TNBC cell lines while IL‐6 was over‐expressed. The positive rate of SOCS3 protein was 32.1% in TNBCs ,63.6% in breast fi‐bromas(P<0.01)and 72.7% in normal breast tissues (P< 0.01);and that of IL‐6 protein was 72.6% in TNBCs ,30.3% in breast fibromas(P< 0.01)and 27.3% in normal breast tissues (P< 0.01).Moreover ,a significant negative correlation was found between the expression of SOCS3 and that of IL‐6 in TNBC(rs = -0.590 ,P<0.01). The expressions of SOCS3 and IL‐6 were markedly associated with tumor size ,lymph node metastasis ,clinical stages and expressions of Ki‐67 and p53(P<0.05) , but not with age ,histological grades or menopausal status(P>0.05).Conclusion The expressions of SOCS3 and IL‐6 are asso‐ciated with the development of TNBC ,suggesting that SOCS3 and IL‐6 can serve as biological markers for evaluating the prog‐nosis ,and therapeutic targets of T NBC.

Objective To investigate the relationship between CD+3CD28+T cell value and chemotherapeutic efficacy in patients with advanced digestive cancer. Methods 38 digestive cancer patients with distant metastatic lesion were included.10 healthy persons were as control group. The absolute counts of CD+3CD28+T cell were measured with flow eytometry in these patients before chemotherapy. All patients received chemotherapy, gastric cancer: DCF regimen (docetaxel plus tegafur and cisplatin, one cycle), colorectal cancer:. Folfox regimen (L-OHP plus tegafur, two cycles), esophageal cancer: mitomycin plus tegafur and cisplatin (two cycles), gallbladder or pancreatic cancer: gemzar plus tegafur and cisplatin (one cycle). According to WHO standard, the chemotherapeutic efficacy was determined, 38 patients were divided into three groups: CR+PR (complete remission plus partial remission), SD (stable of disease) and PD (progress of disease) group. Statistical analysis was used upon all data. Results There was no significant difference in value of CD+3CD28+ T cell between CR+PR group and control group (P=0.549). The value of CD+3CD28+ T cell in SD group and PD group were significantly decreased (P=0.002, P=0.000). The value of CD+3CD28+T cell in CR+PR group was more than that in PD group (P =0.001), while in SD group was more than in PD group (P = 0.044). Conclusion The efficacy is poor, if the value of CD+3CD28+ T cell in patients with advanced digestive cancer is less than that of in healthy persons in peripheral blood. Therefore, the value of CD+3CD28+ T cell is important for the early prediction of the efficacy before chemotherapy.

Objective To establish a numerical model of adsorption and desorption of CO_2 and to study the influencing factors on the removal system. Method A physical model was expressed by mathematical method, then an isothermal adsorption model for simulating molecular sieve was established. Result The working process of a CO_2 removed device was simulated and its result was analized with the established model.The characteristics of the adsorption and desorption was obtained. Conclusion The model describes the processes correctly, the influencing factors like humidity dimension of the bed are considered simultaneously.It remains to be improved by further experimental corrections.

Objective:To investigate the physical morphology of the root canal taper in human maxillary and mandibular permanent first molars.Methods: Forty maxillary and 44 mandibular extracted human permanent first molars were investigated.The roots were dissected transversely from their apical foramen vertical to the long axis of the root,and 1-mm-thick serial sections were made.The root apex side of each section was observed with a stereomicroscope at 30? magnification.The maximum and minimum canal diameters were measured at 0,1,2,and 3 mm from the apical foramen.The physical canal taper in the apical section was calculated.Results: 47.5%-60.0% of the narrowest diameter of root canals were present at the foramen,and 25.0%~42.5% located at the level of 1 mm from apical foramen.Almost all physical configurations of root canals in 3 mm apical region showed a bottleneck-like taper.Canal dia-meters increased rapidly at 1-2 mm with the biggest taper,and the taper had a bigger variance(0.07-0.35).The ring of shrunken bottleneck was formed at the level of 1 mm from apical foramen.The taper of canal in maximal diameter was larger than the one in minimal diameter.Conclusion: The physical morphology of bottleneck-like taper in apical canal was helpful to locate the canal apical stop in root canal treatment.

Objective To establish an HPLC method for determination of the content of paeoniflorin in in Anwei Granules.Methods With Phonomenex C18,150mm?4.60mm,5micron,acetonitrile-water-phosphoric acid(15∶85∶0.2)was used as the mobile phase,The flow rate was 1.0ml/min.The detection wavelength was 230nm.Results The linearity was obtained from 2.52-20.14 ?g(r=0.9990).The average recovery was 99.17 % with RSD=0.91%(n=6).Conclusions The method is sensitive,simple,specific and accurate for determination of astragaloside in Tangshenkang Granules.