No abstract available.
Child* ; Emergencies* ; Emergency Service, Hospital* ; Humans ; Infant* ; Poisoning*

Epstein-Barr virus (EBV) is a potent inducer of polyclonal B lymphocyte proliferation and a tool for the establishment of human B lymphoblastoid cell lines (B-LCLs), which have proven useful for several human immunologic applications. B-LCLs serve as efficient antibody-producing cells and antigen-presenting cells. In spite of these advantages, the cloning efficiency of B-LCLs is less than 1%. In order to generate clones of B-LCLs, we cultured B-LCLs with and without canine stromal cell line, DO64, as feeder cell which was immortalized by transduction of retrovirus encoding E6 and E7 of the human papilloma virus type 16 (HPV-16), which was defined to produce various cytokines including stem cell factor (SCF) and interleukin- 6 (IL-6). After 3 weeks of B-LCLs cultured with DO64, 8.3% and 37.5% in 1 cell and 3 cells per well were efficiently cloned, respectively. There was no significant effect on growing of 8-LCLs without DO64 cells and on high concentration of FBS. The cloning efficiency of B-LCLs transduced by retrovirus cultured with and without DO64 cells was 4.2% and 0% in 3 cells per well, respectively, while that of stable transfectant 33.3% and 8.3% in 1 cell per well, respectively. Our results suggest that the use of DO64 cells as feeder cells might permit the cloning of B-LCLs. This efficient cloning of B-LCLs could be used for the convenient source of autologous antigen-presenting cells expressing foreign antigen for the study of human immune responses in vitro, and for a variety of additional purposes, such as the production of human monoclonal antibodies.
Antibodies, Monoclonal ; Antibody-Producing Cells ; Antigen-Presenting Cells ; Cell Line* ; Clone Cells* ; Cloning, Organism* ; Cytokines ; Feeder Cells* ; Herpesvirus 4, Human ; Humans* ; Lymphocytes ; Papilloma ; Retroviridae ; Stem Cell Factor ; Stromal Cells*

No abstract available.
Humans* ; Retroviridae*

No abstract available.
Danazol* ; Panniculitis*

No abstract available.
Granuloma Annulare ; Rheumatoid Nodule

No abstract available.
Granuloma Annulare ; Rheumatoid Nodule

No abstract available.
Mucinoses*

No abstract available.
Chlorpromazine* ; Hyperpigmentation ; Pigmentation*

No abstract available.
Neurofibromatoses*

BACKGROUND: Data regarding the incidence rates of skin diseases that are held by general hospitals provide readily available sources of information that may be representative of the provinces they serve. The population of Goyang city has increased rapidly. Hence, a statistical study of the latest data is necessary. OBJECTIVE: This study aimed to investigate the prevalence rates of common dermatoses in Goyang city and to compare these with previous findings. METHODS: We reviewed the records of 21,693 outpatients who visited the Department of Dermatology at Myongji Hospital from 2009 to 2013, and we statistically analyzed the findings. RESULTS: Of the 21,693 outpatients' records reviewed, 9,939 (45.8%) were male patients and 11,754 (54.2%) were female patients. Patients most frequently presented with dermatoses in the fourth decade of life (16.0%), followed by the first (15.2%), fifth (15.0%), and third (13.1%) decades of life, and these four age groups comprised 59.3% of the total number of outpatients. The 15 most common dermatoses were dermatophytoses, alopecia, other eczematous conditions, urticaria, herpes zoster infections, viral warts, atopic dermatitis, allergic contact dermatitis, seborrheic dermatitis, acne, irritant contact dermatitis, benign epidermal tumors, melanocytic nevi, molluscum contagiosum, and pruritus. Diseases that occurred frequently during the summer were dermatophytoses, urticaria, irritant contact dermatitis, herpes zoster infections, and molluscum contagiosum. Diseases that occurred frequently during the winter were other eczematous conditions, atopic dermatitis, and melanocytic nevi. Viral warts and acne occurred frequently in summer and winter. Seborrheic dermatitis, dermatophytoses, molluscum contagiosum, viral warts, alopecia, and benign epidermal tumors showed higher frequencies in males than in females, whereas allergic contact dermatitis, irritant contact dermatitis, herpes zoster infections, melanocytic nevi, urticaria, pruritus, acne, and other eczematous conditions showed higher frequencies in females than in males. CONCLUSION: Compared with previous studies, the current population of Goyang city showed higher levels of herpes zoster infections and viral warts.
Acne Vulgaris ; Alopecia ; Dermatitis, Allergic Contact ; Dermatitis, Atopic ; Dermatitis, Contact ; Dermatitis, Seborrheic ; Dermatology ; Female ; Herpes Zoster ; Hospitals, General ; Humans ; Incidence ; Male ; Molluscum Contagiosum ; Nevus, Pigmented ; Outpatients ; Prevalence ; Pruritus ; Skin Diseases* ; Statistics as Topic* ; Tinea ; Urticaria ; Warts