1.Giant Infantile Hemangioma Treated with Beta-blocker with Intermittent Triamcinolone Intralesional Injection.
Gil HAN ; Jae We CHO ; Kyu Suk LEE
Korean Journal of Dermatology 2015;53(8):658-659
No abstract available.
Hemangioma*
;
Injections, Intralesional*
;
Propranolol
;
Triamcinolone*
2.Erratum: Author's Name Correction. Dapsone Hypersensitivity Syndrome with EBV Reactivation.
Gil HAN ; Kyu Suk LEE ; Jae We CHO
Korean Journal of Dermatology 2014;52(6):447-447
Author list should be corrected.
3.Dapsone Hypersensitivity Syndrome with EBV Reactivation.
Jae We CHO ; Kyu Suk LEE ; Gil HAN
Korean Journal of Dermatology 2014;52(5):358-360
No abstract available.
Dapsone*
;
Herpesvirus 4, Human*
;
Hypersensitivity*
4.IS6110 based DNA Fingerprinting of Mycobacterium tuberculosis Isolated from The Nationwide Tuberculosis Prevalence Survey in 1995.
Sang Jae KIM ; Young Kil PARK ; Sang Hyun CHO ; Gil Han BAI ; Yeun KIM
Journal of the Korean Society for Microbiology 1998;33(5):475-484
One hundred and thirty-eight strains of Mycobacterium tuberculosis isolated from the 7th nationwide tuberculosis prevalence survey in 1995 were subjected to the restriction fragment length polymorphism (RFLP) analysis using IS6110 probe to define the representative fingerprinting patterns of Korean strains of M. tuberculosis and to evaluate the usefulness of DNA fingerprinting in tracing the transmission link of M. tuberculosis. Among 138 strains, 129 different IS6l10 RFLP types were identified. The number of bands in IS6110 RFLP types diversed from 1 to 20, and the majority (75%) was 9 to 14 bands. The RFLP patterns of 8 out of 15 strains isolated from the follow-up survey of one and half year later after the 7th national TB prevalence survey were unchanged when compared with previous RFLP patterns. Fifteen (11%) out of 138 strains were grouped in 6 IS6110 clusters; 2 with 10 copies, 2 with 12 copies, 1 with 14 copies, and 1 with 17 copies. These clusters were unable to be subclassified by IS1081 or (GTG) probes except one cluster by pTBN12 probe. The transmission links of 2 clusters were deducible; one from household and another from neighborhood, but those of remaining clusters were unclear because they had no contact one another. The results suggest that vigorous transmissions in tuberculosis are still progressing in Korea.
Dermatoglyphics
;
DNA Fingerprinting*
;
DNA*
;
Family Characteristics
;
Follow-Up Studies
;
Korea
;
Mycobacterium tuberculosis*
;
Mycobacterium*
;
Polymorphism, Restriction Fragment Length
;
Prevalence*
;
Residence Characteristics
;
Tuberculosis*
5.HIV - 1 p24 Expression in BCG and the Immunogenicity of Recombinant BCG in Experimental Animals.
Sang Hyun CHO ; Han Jeong MYOUNG ; Hye Rhan JEONG ; Gil Han BAI ; Sang Jae KIM ; Yong Soo BAE
Korean Journal of Immunology 1997;19(2):289-300
HIV-1 p24 was cloned into multiple cloning site of pMV261, extrachromosomal expression vectors carrying BCG replication origin and BCG-specific heat-shock promoter, and then introduced into BCG and E. coli. Western blot experiments showed that the p24 efficiently expressed in recombinant BCG (rBCG), but not in E. coli. Recombinant p24 expression induced by a single heat-shock of rBCG was maintained longer than 3 weeks. Immunoblot experiments with intact rBCG did not show any distinctive positive signal, suggesting that the recombinant protein was not secreted or exposed at the surface of BCG. The guinea pigs immunized with live rBCG showed delayed type hypersensitivity (DTH) by the systemic area as well as an effective humoral immunity, suggesting that tbis rBCG is believed to elicit eKcient immune responses against p24, even though the expression is restricted only in the cytoplasm as reported previously with other antigen. These results demonstrate that BCG can be developed as a live recombinant vaccine vector against a broad spectrum of infectious disease.
Animals*
;
Blotting, Western
;
Clone Cells
;
Cloning, Organism
;
Communicable Diseases
;
Cytoplasm
;
Guinea Pigs
;
HIV*
;
HIV-1
;
Hypersensitivity
;
Immunity, Humoral
;
Mycobacterium bovis*
;
Replication Origin
6.Evaluation of Continuing Education Program to Enhance Competency for Hospice Volunteers: An Exploratory Mixed-Methods Design
Minjeong SEO ; Han A CHO ; Sang Mi HAN ; Youngshim KO ; Cho Rong GIL
Korean Journal of Hospice and Palliative Care 2019;22(4):185-197
PURPOSE: Hospice volunteers are serving an invisible yet pivotal role in the hospice and palliative care team. This study investigated how effectively a continuing education program could enhance hospice volunteers' competency.METHODS: A total of 20 hours (four hours per week) of training was provided to 30 hospice volunteers who participated in the continuing education for hospice volunteers. Efficiency of the education was analyzed with an exploratory mixed-methods design. For quantitative analysis, the volunteers were asked, before and after the training, about their attitudes towards hospice care, what makes a meaningful life, self-efficacy and satisfaction with their volunteer service. Descriptive statistics, paired t-tests, and Wilcoxon signed-rank test were performed using SPSS Window 20.0. For qualitative research, participants were placed in three groups for a focus group interview, and data were analyzed by content analysis.RESULTS: A quantitative study result shows that this training can significantly affect hospice volunteers' attitudes and improve their self-efficacy. A qualitative study result shows that participants wanted to receive continuous education from the physical/psychosocial/spiritual aspects to better serve end-of-life patients and their family members even though they have to spare significant time for the volunteer service. They wanted to know how to take good care of patients without getting themselves injured and how to provide spiritual care.CONCLUSION: The continuing education program reflecting volunteers' requests is strongly needed to improve their competency. An effective continuing education requires continuous training and support in areas where hospice volunteers are interested in. A good alternative is to combine web-based and hands-on training, thereby allowing hospice volunteers freely take training that suits their interest.
Education
;
Education, Continuing
;
Focus Groups
;
Hospice Care
;
Hospices
;
Hospital Volunteers
;
Humans
;
Palliative Care
;
Qualitative Research
;
Volunteers
7.A Case of Disseminated Cryptococcosis with Skin Involvement in a Rheumatic Arthritis Patient During Long-term Tacrolimus Therapy.
Gil HAN ; Kyu Suk LEE ; Jae We CHO
Korean Journal of Medical Mycology 2013;18(3):76-81
Cryptococcosis is the infection caused by the encapsulated yeast Cryptococcus species, occuring most frequently in immunocompromised hosts. Cutaneous cryptococcosis occurs in 10~15% of disseminated cases and its clinical manifestation is variable. The patient was a 73-year-old female, who had been taking a tacrolimus 3 mg/day for 2 years due to rheumatic arthritis. She had an erythematous ulcerated lesion with elevated border on right shoulder and left back, and painful subcutaneous nodules on left chest. Histopathologic examination of skin showed typical spores with capsules in dermis and chest X-ray showed fungal ball in lung. She was successfully treated by systemic amphotericin B and fluconazole. Herein, we report a case of disseminated cryptococcosis with skin involvement during long-term tacrolimus therapy.
Aged
;
Amphotericin B
;
Capsules
;
Cryptococcosis*
;
Cryptococcus
;
Dermis
;
Female
;
Fluconazole
;
Humans
;
Immunocompromised Host
;
Lung
;
Rheumatic Fever*
;
Shoulder
;
Skin*
;
Spores
;
Tacrolimus*
;
Thorax
;
Ulcer
;
Yeasts
8.Increased Expression of Type I Collagen, MMP-1, and MMP-2 in Platelet-rich Plasma-treated Human Skin Fibroblasts.
Gil HAN ; Sang Pyo KIM ; Jae We CHO
Korean Journal of Dermatology 2015;53(4):290-297
BACKGROUND: Platelet-rich plasma (PRP) treatment is a promising tool for dermal tissue regeneration. PRP combined with subcision can synergistically induce dermal tissue regeneration. OBJECTIVE: The purpose of this study was to investigate the effects of PRP on the proliferation and migration of skin fibroblasts, as well as on the type I collagen, matrix metalloproteinase (MMP)-1, and MMP-2 expression in these skin cells. The effect of PRP with subcision on the expression of TGF-beta1 was also investigated in an animal model. METHODS: Human skin fibroblasts were treated with various concentrations of PRP. The proliferation and migration rate of the cells were evaluated by the trypan blue exclusion method and scratch assay, respectively. The expression levels of type I collagen, MMP-1, and MMP-2 were analyzed by western blot or RT-PCR. In addition, the activity levels of MMP-1 and MMP-2 were studied by zymography. Finally, we treated the animal back with PRP, subcision, or PRP with subcision. The specimens were evaluated by H&E, Masson-trichrome, and TGF-beta1 immunohistochemical staining. RESULTS: Data from this study showed that PRP more effectively promoted the migration and proliferation of cells in a dose-dependent manner. The expression levels of type I collagen, MMP-1, and MMP-2 were increased in PRP-treated fibroblasts at the protein and mRNA levels. The in vivo study revealed that the expression of TGF-beta1 was prominently increased by co-treatment with PRP and subcision rather than by treatment with either PRP or subcision alone. CONCLUSION: PRP treatment promoted fibroblast migration and proliferation, and increased the expression of type I collagen, MMP-1, MMP-2, and TGF-beta1. Therefore, PRP co-application with subcision is an effective method for dermal remodeling and can be a good treatment option for depressed acne scars.
Acne Vulgaris
;
Animals
;
Blotting, Western
;
Cicatrix
;
Collagen Type I*
;
Fibroblasts*
;
Humans
;
Models, Animal
;
Platelet-Rich Plasma
;
Regeneration
;
RNA, Messenger
;
Skin*
;
Transforming Growth Factor beta1
;
Trypan Blue
9.Increased Expression of Type I Collagen, MMP-1, and MMP-2 in Platelet-rich Plasma-treated Human Skin Fibroblasts.
Gil HAN ; Sang Pyo KIM ; Jae We CHO
Korean Journal of Dermatology 2015;53(4):290-297
BACKGROUND: Platelet-rich plasma (PRP) treatment is a promising tool for dermal tissue regeneration. PRP combined with subcision can synergistically induce dermal tissue regeneration. OBJECTIVE: The purpose of this study was to investigate the effects of PRP on the proliferation and migration of skin fibroblasts, as well as on the type I collagen, matrix metalloproteinase (MMP)-1, and MMP-2 expression in these skin cells. The effect of PRP with subcision on the expression of TGF-beta1 was also investigated in an animal model. METHODS: Human skin fibroblasts were treated with various concentrations of PRP. The proliferation and migration rate of the cells were evaluated by the trypan blue exclusion method and scratch assay, respectively. The expression levels of type I collagen, MMP-1, and MMP-2 were analyzed by western blot or RT-PCR. In addition, the activity levels of MMP-1 and MMP-2 were studied by zymography. Finally, we treated the animal back with PRP, subcision, or PRP with subcision. The specimens were evaluated by H&E, Masson-trichrome, and TGF-beta1 immunohistochemical staining. RESULTS: Data from this study showed that PRP more effectively promoted the migration and proliferation of cells in a dose-dependent manner. The expression levels of type I collagen, MMP-1, and MMP-2 were increased in PRP-treated fibroblasts at the protein and mRNA levels. The in vivo study revealed that the expression of TGF-beta1 was prominently increased by co-treatment with PRP and subcision rather than by treatment with either PRP or subcision alone. CONCLUSION: PRP treatment promoted fibroblast migration and proliferation, and increased the expression of type I collagen, MMP-1, MMP-2, and TGF-beta1. Therefore, PRP co-application with subcision is an effective method for dermal remodeling and can be a good treatment option for depressed acne scars.
Acne Vulgaris
;
Animals
;
Blotting, Western
;
Cicatrix
;
Collagen Type I*
;
Fibroblasts*
;
Humans
;
Models, Animal
;
Platelet-Rich Plasma
;
Regeneration
;
RNA, Messenger
;
Skin*
;
Transforming Growth Factor beta1
;
Trypan Blue
10.Efficiency of different primers in polymerase chain reacion to detect mycobacterium tuberculosis in clinical specimens.
Myung Sup SHIM ; Sung Youn LEE ; Sang Hyun CHO ; Young Kil PARK ; Gil Han BAI ; Sang Jae KIM
Journal of the Korean Society for Microbiology 1993;28(5):391-395
No abstract available.
Mycobacterium tuberculosis*
;
Mycobacterium*