Objective To research the influence of nursing intervention on anxiety state and postoperative complications for IABP to the patients received emergent percutaneous coronary intervention.Methods Eighty patients received emergent percutaneous coronary intervention were randomly divided into the experimental group (n =40) and controls (n =40). The controls received single conventional nursing while the patients in the experimental group were given directed psychological and behavioral interference besides the conventional routine care. Self-rating anxiety scale (SAS) was employed to evaluate the difference on anxiety state of the patients in both groups before and after interference. The incidences of postoperative complications such as bleeding in the paracentetic location were compared between the two groups. Results The incidences of anxiety, bleeding in the postoperative paracentetic location and hypostatic pneumonia and the degree of anxiety were obviously decreased in the experimental group compared to the controls after psychological and behavioral interference (x2 = 21.33,5.16,4.50;P ＜ 0. 05 ). Conclusions The psychological and behavioral interference not only obviously alleviated the incidence and degree of anxiety but decreased the incidences of postoperative complications. All these established a satisfactory basis for the success of operations.

Objective To explore the risk factors of the infection in elderly patients with total hip replacement in Operating Room and study the effects of the targeted nursing interventions to the infection. Methods A total of 296 patients with total hip replacement in our hospital from January 2001 to December 201 3 were included and divided into infection group and control group. Infection group contained 1 02 patients with surgical incision infection and control group contained 1 94 patients without surgical incision infection.The demographic data,medical history,blood index,Harris function score,operation situation and postoperative drainage time were recorded.Infection diagnosis depended on “Diagnose Standard of Nosocomial Infection”.The risk factors were analyzed by single factor analysis and step-wise Logistic multiple regression analysis.The targeted nursing interventions were proposed.The effects of the targeted nursing interventions were studied in 56 patients from February 201 4 to August 201 5.Results Single factor analysis showed that hemoglobin concentrations, joint operations, operative character, visiting during operations and operative time were significantly different between infection group and control group (P <0.01 ).Logistic multiple regression analysis showed that hemoglobin concentrations,joint operations,operative character,visiting during operation and operative time were relative risk factors of postoperative infection (P <0.01 ).The infection ratio of 56 patients with nursing interventions from February 2014 to August 2015 was 17.8% (1 0 /56),which was significantly decreased comparing with the ratio 34.5% (102 /296)from January 2001 to December 201 3 (P <0.05 ).Conclusions Low hemoglobin concentrations,joint operations, emergency operations,visiting during operation and long operative time are risk factors of postoperative infection in elderly patients with total hip replacement in Operating Room.The targeted nursing interventions can reduce the infection ratio and deserves further clinical promoting.

OBJECTIVETo study the effect of palmitate on toll-like receptor 4 (TLR4) expression and signaling in vascular endothelial cells.

METHODSPig iliac endothelial cells (PIECs) were incubated with palmitate. TLR4 gene expression levels were measured by quantitative real-time PCR, and TLR4 and IκBα protein expressions by Western blotting. The expression levels of TLR4 protein on the surface of PIECs were quantified using flow cytometry. ELISA was employed to detect tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) concentrations in the cell medium.

RESULTSPalmitate treatment significantly increased TLR4 mRNA and protein expression levels in PIECs compared with those in the control cells (4.73∓0.61 vs 1.25∓0.90, P<0.05; 5.79∓0.05 vs 4.07∓0.31, P<0.05). The expression levels of TLR4 on the cell surface significantly increased (38.070∓3.907 vs 29.390∓1.072, P<0.05), while IκBα protein level was significantly lowered in PIECs after palmitate treatment as compared with those in the control cells (2.04∓0.22 vs 3.98∓0.18, P<0.05). Palmitate treatment significantly elevated TNF-α (2.52∓0.30 vs 1.38∓0.26, P<0.05) and IL-6 (IL-6: 3.28∓0.32 vs 1.44∓0.28, P<0.05) concentrations in the cell culture medium.

CONCLUSIONPalmitate can enhance TLR4 expression and signaling in porcine vascular endothelial cells.

Animals ; Blotting, Western ; Endothelial Cells ; drug effects ; metabolism ; I-kappa B Proteins ; metabolism ; Interleukin-6 ; metabolism ; NF-KappaB Inhibitor alpha ; Palmitates ; pharmacology ; RNA, Messenger ; Real-Time Polymerase Chain Reaction ; Signal Transduction ; Swine ; Toll-Like Receptor 4 ; metabolism ; Tumor Necrosis Factor-alpha ; metabolism

Objective To explore functional exercise supervision table application effect in patients with femoral neck fracture. Methods During June 2012 to June 2013 in our hospital Orthopedic Ward, a total of 92 elderly patients with femoral neck fracture had been chosen as the research object, and divided into observation group and control group on average according to the random number table method. The patients of control group underwent routine care and treatment, and health education, while the patients of observation group were taken routine nursing care, nursing intervention and functional exercise supervision table. We compared hip joint function, length of hospital stay, and complications between two groups after intervention. Results The patients′scores of Harris joint function had no statistical significance between two groups at the time of discharge (P>0. 05), but postoperative 3 months and 6 months scores compared between two groups, the scores of observation group were (76. 5 ± 4. 5) and (85. 2 ± 4. 1) higher than (70. 1 ± 4. 8) and (80. 9 ± 4. 0) of the control group (t= -3. 658, -2. 986;P<0. 05). The postoperative length of hospitalization in the observation group was obviously shorter than the control group (18. 6 ± 4. 6) d vs. (23. 2 ± 5. 1) d, and the incidence of complications was lower than that in the control group (χ2/t=2. 861, 3. 265, 8. 236, 11. 235, 9. 362, 4. 265, 6. 325, 6. 325, 2. 986;P<0. 05). Conclusions Functional exercise supervision table makes health education content documented by forms which can be supervised by nursing manager, strengthen the nurse′s sense of responsibility, improve the patient′s execution and rehabilitation, and can reduce the hospitalization time and the incidence of complications.

OBJECTIVETo investigate the safety and effectiveness of neonatal ABO or Rh(D) by using compatible blood transfusion through retrospective analysis of data from cases received compatible blood transfusion and type matched blood transfusion.

METHODSThe clinical data of 26 cases of neonatal compatible blood transfusion in Chinese Nanchang area from January 2014 to October 2016 were collected, and 26 cases of neonatal type-matched blood transfusion were selected according to ratio of 1:1 cases. The efficiency and safety index of 26 patients compatible blood transfusion were compared with that of type-matched blood transfusion. The efficiency indexes included: patients' basic characteristics, red blood cell (RBC) count, hemoglobin (Hb) level, hematocrit (Hct), and the safety indexes contain Hb level and indirect bilirubin (IBiL) value before and after blood transfusion, irregular antibody screening, direct antiglobulin test (DAT) results and the adverse reactions of blood transfusion.

RESULTSThe age, sex, days of hospitalization between compatible blood transfusion and type matched blood transfusion were not statistically significantly different (P>0.05). The Hb level before transfusion, blood transfusion volume and the increase of Hb, Hct and RBC were not statistically significantly different between two groups (P>0.05). The values of Hb, Hct and RBC in 2 groups significantly increased at the day 1 after blood transfusion (P<0.05). No blood transfusion adverse reaction occurred in 2 groups. The IBiL value significantly decreased in compatible blood transfusion patients at the day 1 after blood transfusion (P<0.05). No new irregular antibodies had been detected after transfusion in all patients, and the others' DAT and screening for irregular antibodies were negative except 22 patients with neonatal hemolysis. The values of Hb and IBiL statistically significantly differenence were not in 12 patients between 1d, 3d, 7d after blood transfusion (P>0.05).

CONCLUSIONThe efficiency and safety between compatible blood transfusion and type matched blood transfusion are the same in neonatal blood transfusion. Compatible blood transfusion is a safe and effective in clinical blood transfusion.

OBJECTIVETo investigate the reversal effect of dihydromyricetin(DMY) on drug resistance of K562/A02 cells to adriamycin and explore its possible mechanism.

METHODSK562 and K562/A02 cells were treated with DMY (5, 10, 20, 40, 60, 80 and 100 mg/L) and ADM (100-0.05 mg/L) for 48 h. The viability of K562 cells and K562/A02 cells was tested and the reversal effect of DMY on drug resistance of K562/A02 cells to adriamycin was analyzed by MTT. The relative concentration of ADM in cells was measured by flow cytometry. Protein expressions of drug resistance related genes including P-glycoprotein (P-gp), multidrug resistance associated protein 1 (MRP1), glutathione transferase π (GSTπ) and BCL-2 were measured by Western Blot.

RESULTSThe proliferation of K562 and K562/A02 cells was significantly decreased by DMY in dose-dependent manner as compared with control group (r1=0.37, r2=0.38). The ICof ADM on K562 and K562/A02 cells were 71.23±6.51 and 72.88±5.49 mg/L respectively. DMY (5, 10 and 20 mg/L) was low cytotoxicity. DMY (5, 10 and 20 mg/L) enhanced the sensitivity of K562/A02 cells to ADM in dose-dependent manner (r1=-0.62, r2=-0.71) and the reversal multiples was from 1.38 to 28.591. The relative concentrations of ADM in K562/A02 of DMY (5, 10 and 20 mg/L) group cells were significantly increased in dose-dependent manner compared with the control group (r=0.34). Compared with the control group, the expressions of drug resistance related protein P-gp, MRP1, GSTπ and BCL-2 were significantly decreased in dose-dependent manner in DMY (5, 10 and 20 mg/L) group (r1=-0.41, r2=-0.37, r3=-0.58, r=-0.46). Compared with the ADM group, the protein expressions of drug resistance related genes P-gp, MRP1, GSTπ and BCL-2 in DMY (5, 10 and 20 mg/L)+ADM group were significantly decreased in dose-dependent manner (r1=-0.55, r2=-0.41, r3 =-0.38, r4=-0.44).

CONCLUSIONDMY enhances the sensitivity of K562/A02 cells to ADM, its mechanism may be related with decrease of P-gp, MRP1, GSTπ and BCL-2 expressions.