Aim To investigate the effects of com-bined treatment of SAHA and TRAIL on human breast cancer ER positive cell line MCF-7 . Methods MCF-7 cells were treated with SAHA and/or TRAIL. The inhibitory rates were detected by real-time cell prolifer-ation assays. Morphology changes of MCF-7 cells were observed through time-lapse live cell imaging acquisi-tion. Results Real-time cell proliferation assays showed that the anti-tumor efficacy of SAHA was sig-nificantly enhanced in combination with TRAIL. The results of time-lapse live cell imaging acquisition dem-onstrated that, with treatment of SAHA and TRAIL, the growth inhibition of MCF-7 cells was more obvious than that of in TRAIL or SAHA treatment alone. Con-clusion The combination treatment of SAHA and TRAIL has a synergistic effect of growth inhibition on breast cancer MCF-7 cells.

Objective To analyze the effects of gentongping pill combined with physiotherapy on the patients with vertebral artery type of cervical spondylosis. Methods 134 patients with vertebral artery type of cervical spondylosis were grouped into two groups, the observation group and the control group. The two groups were treated with physiotherapy and the observation group was treated with Gentongping pill. The effects in the two groups were evaluated. Results The total efficiency in the observation group was higher than that in the control group(P<0.05). After treatment, the symptom score, CASCS score, mean blood flow velocity in left vertebral artery and right vertebral artery, TNF-α and IL-6 level in the observation group were better than those in the control group (all P<0.05). There was no obvious adverse reaction in the two groups. Conclusion Itcan improve the therapeutic effect which Gentongping pill is used on the treatment of patients with vertebral artery type of cervical spondylosis.

The medical equipment benefit evaluation analysis has become an important constituent of cost and medical equipment management. It can not only use the economic benefit index to estimate the service condition of equipment, but also form the comprehensive evaluation system with basic of economic benefit evaluation and auxiliary of science study. Based on comprehensive evaluation result, to search the weakness of equipment utilization and promote the overall development of hospitals and clinics orderly.

Experiment of pharmacy will inevitably cause a waste of resources and environmental pollution. Therefore, it is necessary to explore some green teaching method in the experiment of pharma-ceutics. Based on the long-term teaching experience, this paper took the preparation of yiyuan powder, the cold fever granules and tables, and subing dropping as examples to compare the differences between the traditional teaching method and the green teaching method in the aspects of improving the basic operating skills, saving materials and reagents and using the micro experiments. The results showed that compared with the conventional teaching, green teaching method in pharmaceutics experiment process can significantly reduce the reagent and the amount of raw materials, so as to enables students to cultivate green, energy saving, environmental awareness while mastering the pharmaceutical theory and technology.

10.3969/j.issn.2095-4344.2013.25.026

MicroRNAs are a kind of small endogenous non-protein-coding single-stranded RNA,which are 18 to 25 nucleotides long.They either regulate expression of specific genes through the degradation of mRNA or repress expression of target protein of mRNA postoperation by binding with the 3' untranslated region of targeting mRNAs.It produces important physiological effects on cell proliferation, differentiation and apoptosis.The use of experimental biology and bioinformatics methods for research of tumor-associated miRNAs has become research focus.In this paper, the role of miRNAs in the oncogenesis, development, metastasis and prognosis of gallbladder cancer is mainly introduced.

Objective To design an intravenous indwelling needle in battle field aid.Methods Several concavity were made on the hollow tube inner surface posterior puncture needle case,and some corresponding convexity uplifted peripheral needle handle pistons posterior puncture needle core.Revolution could be avoided because the needle handle pistons were just embedded in hollow tube concavity.It was convenient to operate and decreased the operator's orientation transformation by the needle's handhold vane adopting rectangle instead of butterfly shape.Results The improved puncture needle was stable and the rate of success of intravenous puncture was increased.Conclusion The improved venous retaining needle can be applied in battle field aid.

Toll-like receptors (TLRs), a large family consisting of at least 10 members, are evolutionarily conserved to recognize pathogen-associated molecular patterns (PAMPs). TLRs activation not only initiates innate immunity, but also regulates enhance antigen-specific acquired immunity, and thus associates innate and adaptive immunity. In recent years, studies on the TLRs signaling, especially their negative regulation, rapidly progressed. TLRs signaling pathway and their roles in regulating immune responses against invading pathogens were reviewed.

OBJECTIVE:To investigate the regulation effects of oleanolic acid on the mitochondrial nitric oxide synthase(mt-NOS) in human umbilical vein endothelial cells (HUVECs) with oxidative damage. METHODS:HUVECs in exponential phase were divided into normal group,model group and oleanolic acid low,medium and high dose groups(5,20 and 35 μmol/L). After drug acting for 24 h,all groups were given culture solution containing 100 μg/ml ox-LDL to reproduce oxidative damage except normal group. CCK-8 was used to detect cell viability. The mitochondria in cells were extrated,enzyme chemical method was used to detect mtNOS activity and mtNO content,fluorescence microplate method was used to detect fluorescence intensity of reactive oxygen species(ROS),and western blot was used to detect expression of cytochrome C(Cyto-C). RESULTS:Compared with nor-mal group,the cell viability in model group was decreased;mtNOS activity,mtNO content,ROS fluorescence intensity and Cy-to-C protein expression were increased,with significant differences (P<0.05). Compared with model group,the cell viability in oleanolic acid low,medium and high dose groups was increased;mtNOS activity,mtNO content,ROS fluorescence intensity and Cyto-C protein expression were decreased,with significant differences(P<0.05),and they had positive correlation with concentra-tions. CONCLUSIONS:Oleanolic acid can decrease the mtNOS activity of HUVECs,reduce the production of mtNO and Cyto-C, by a mechanism that may be related to the decrease of ROS expression.

Constitution is an important factor to determine the susceptibility and the occurrence of disease. This article analyzed causes and characteristics of damp-heat constitution, the change of damp-heat constitution to damp-heat syndrome, the relationship between damp-heat constitution and the occurrence and development of diabetes mellitus. It reached the conclusion that damp-heat constitution is one of the important factors of diabetes mellitus, that damp-heat syndrome is always accompanied with the development of diabetes, and proposed the treatment methods of lifestyle intervention, elimination of pathogens through purgation and diuresis, nourishing yin and dredging the collaterals. It also proposed the new ideas and thoughts of early intervention in damp-heat constitution and distinguishing constitution therapy for the prevention and treatment of diabetes mellitus.