OBJECTIVE: To determine the heat effect on anti-inflammatory action in knee osteoarthritis in experimental rabbit during sand treatment in Uyghur medicine. METHODS: We established an indoor experimental platform for the sand treatment and collected the continuously changing data skin temperature in the rabbits and the temperature of each layer of sand.All the data were collected by the apparatus of 2700 type multichannel data collection. EMK was injected into the joint of right knees, and then the rabbits were randomly divided into 3 groups: a sand therapy group, a fenbid therapy group,and a blank control group. From the 15th day after the articular injection to the 30th day,the rabbits were treated with sand once daily; fenbid, once daily; and nothing in the 3 groups,respectively. At the end of the experiment, the serum and the joint fluid of the diseased joints were obtained to test IL-1beta, TNF-alpha,and NO level; the cartilage was obtained for the histopathologic measurement. RESULTS: At the beginning,skin temperature went up,then fell down gradually. Compared with the fenbid group and the blank control group, the severity of cartilage in the sand therapy group declined significantly. The IL-beta and TNF-alpha level in the joint fluid and NO level in the serum of the sand therapy group decreased significantly. CONCLUSION The sand treatment is effective,which may be related to the decreasing of cytokine in the local joints by the heat effect.
Animals ; Hot Temperature ; therapeutic use ; Interleukin-1beta ; metabolism ; Male ; Medicine, Chinese Traditional ; methods ; Nitric Oxide ; blood ; Osteoarthritis ; metabolism ; therapy ; Rabbits ; Random Allocation ; Silicon Dioxide ; Skin Temperature ; Tumor Necrosis Factor-alpha ; metabolism