1.Change of taste preference and taste bud after unilateral lingual nerve transection in rat.
Yoon Tae KIM ; Seung Ho JEON ; Hak Ryol YEOM ; Jin Han KANG ; Kang Min AHN ; Sung Min KIM ; Jeong won JAHNG ; Kyung Pyo PARK ; Jong Ho LEE
Journal of the Korean Association of Oral and Maxillofacial Surgeons 2005;31(6):515-525
PURPOSE OF STUDY: Lingual nerve damage can be caused by surgery or trauma such as physical irriatation, radiation, chemotherapy, infection and viral infection. Once nerve damage occurred, patients sometimes complain taste change and loss of taste along with serious disturbance of tongue. The purpose of this study was to evaluate the effects of unilateral lingual nerve transection on taste as well as on the maintenance of taste buds. MATERIALS AND METHODS: Male Sprague-Dawley rats weighing 220-250g received unilateral transection of lingual nerve, subjected to the preference test for various taste solutions (0.1M NaCl, 0.1M sucrose, 0.01M QHCl, or 0.01M HCl) with two bottle test paradigm at 2, 4, 6, or 8 weeks after the operation. Tongue was fixed with 8% paraformaldehyde. After fixation, they were observed with scanning electron microscope(JSM-840A(R), JEOL, JAPAN) and counted the number of the dorsal surface of the fungiform papilla for changes of fungiform papilla. And, Fungiform papilla were obtained from coronal sections of the anterior tongue(cryosection). After cryosection, immunostaining with Galpha gust(I-20)(Santa Cruz Biotechnology, USA), PLCbeta2(Q-15)(Santa Cruz Biotechnology, USA), and T1R1(Alpha Diagnostic International, USA) were done. Immunofluorescence of labeled taste bud cells was examined by confocal microscopy(F92-300., Olympus, JAPAN). RESULTS: The preference score for salty and sweet tended to be higher in the operated rats with statistical significance, compared to the sham rats. Fungiform papilla counting were decreased after lingual nerve transaction. In 2 weeks, maximum differences occurred. Gustducin and T1R1 expressions of taste receptor in 2 and 4 weeks were decreased. PLCbeta2 were not expressed in both experimental and control group. CONCLUSION: This study demonstrated that the taste recognition for sweet and salty taste changed by week 2 and 4 after unilateral lingual nerve transection. However, regeneration related taste was occurred in the presence of preserving mesoneurial tissue and the time was 6 weeks. Our results demonstrated that unilateral lingual nerve damage caused morphological and numerical change of fungiform papilla. It should be noted in our study that lingual nerve transection resulted in not only morphological and numerical change but also functional change of fungiform papillae.
Animals
;
Biotechnology
;
Drug Therapy
;
Fluorescent Antibody Technique
;
Humans
;
Lingual Nerve Injuries*
;
Lingual Nerve*
;
Male
;
Rats*
;
Rats, Sprague-Dawley
;
Regeneration
;
Sucrose
;
Taste Buds*
;
Tongue
2.Long term evaluation of volume change in free vascularized fibular flap mandible reconstruction.
Yoon Tae KIM ; Seung Ho JEON ; Hak Ryol YEOM ; Kang Min AHN ; Hoon MYOUNG ; Soon Jung HWANG ; Byoung Moo SEO ; Jin Young CHOI ; Pill Hoon CHOUNG ; Myung Jin KIM ; Jong Ho LEE
Journal of the Korean Association of Oral and Maxillofacial Surgeons 2006;32(2):138-141
INTRODUCTION: In recent years, vascularized, i.e., living bone grafts, have been widely applied in the field of oral and maxillofacial surgery, as a method of treatment of congenital or acquired non-unions, and a large defects in mandible. The vascularized fibular graft has been especially used for this purpose because of its shape and mechanical strength. The postoperative hypertrophy of grafted fibula is of particular interest to us. MATERIAL AND METHODS: This study was undertaken to determine the volume change(indirect methods) and radiographic appearance of a free vascularized fibular graft as it responds to the mechanical and physiologic features of its new environment. In order to elucidate the long term effect on fibular mass after mandibular reconstruction, change in various method of volume change was utilized as indirect measure of change in long-term. RESULTS: The younger the patient, the more prominent and rapid the hypertrophy of the graft. the hypertrophy of the graft never exceeded the diameter of the recipient bone, except for callus enlargement after stress fracture of the grafted bone. CONCLUSION: Etiologic explanations for this phenomenon have not been clarified in the previeous literature. some of the factors implicated include a periosteal reaction or new bone formation, as seen at the onset of bone union after a fracture in a child, a reaction to the mechanical loading on the graft and a reaction to the circulatory changes resulting from the grafting procedure.
Bony Callus
;
Child
;
Fibula
;
Fractures, Stress
;
Humans
;
Hypertrophy
;
Mandible*
;
Mandibular Reconstruction
;
Osteogenesis
;
Surgery, Oral
;
Transplants
3.Long term evaluation of volume change in free vascularized fibular flap mandible reconstruction.
Yoon Tae KIM ; Seung Ho JEON ; Hak Ryol YEOM ; Kang Min AHN ; Hoon MYOUNG ; Soon Jung HWANG ; Byoung Moo SEO ; Jin Young CHOI ; Pill Hoon CHOUNG ; Myung Jin KIM ; Jong Ho LEE
Journal of the Korean Association of Oral and Maxillofacial Surgeons 2006;32(2):138-141
INTRODUCTION: In recent years, vascularized, i.e., living bone grafts, have been widely applied in the field of oral and maxillofacial surgery, as a method of treatment of congenital or acquired non-unions, and a large defects in mandible. The vascularized fibular graft has been especially used for this purpose because of its shape and mechanical strength. The postoperative hypertrophy of grafted fibula is of particular interest to us. MATERIAL AND METHODS: This study was undertaken to determine the volume change(indirect methods) and radiographic appearance of a free vascularized fibular graft as it responds to the mechanical and physiologic features of its new environment. In order to elucidate the long term effect on fibular mass after mandibular reconstruction, change in various method of volume change was utilized as indirect measure of change in long-term. RESULTS: The younger the patient, the more prominent and rapid the hypertrophy of the graft. the hypertrophy of the graft never exceeded the diameter of the recipient bone, except for callus enlargement after stress fracture of the grafted bone. CONCLUSION: Etiologic explanations for this phenomenon have not been clarified in the previeous literature. some of the factors implicated include a periosteal reaction or new bone formation, as seen at the onset of bone union after a fracture in a child, a reaction to the mechanical loading on the graft and a reaction to the circulatory changes resulting from the grafting procedure.
Bony Callus
;
Child
;
Fibula
;
Fractures, Stress
;
Humans
;
Hypertrophy
;
Mandible*
;
Mandibular Reconstruction
;
Osteogenesis
;
Surgery, Oral
;
Transplants
4.Implant installation using vertical distraction osteogenesis at a severely atrophied edentulous mandible
Hak Ryol YEOM ; Seung Ho JEON ; Yoon Tae KIM ; Jun Young PAENG ; Kang Min AHN ; Hoon MYUNG ; Soon Jung HWANG ; Byoung Moo SEO ; Jin Young CHOI ; Jong Ho LEE ; Pill Hoon CHOUNG ; Myung Jin KIM
Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons 2006;28(2):154-165