The patients with cor pulmonale in release stage have been treated and cared with many methods.The results showed that there are obvious recuperatio of respiratory symptoms and lung function and evident strength of the immune function of the body and movement of diaphragm in the patients of treatment group with cor pulmonale compared with control group.The studies suggested that the rehabilitation treatment and care plays an important role in treating patients with cor pulmonale,particularly postponing and prevention acute attack.

Objective To study the influence of infection again to hepatifis B sufferer get disease turn into by human cytomegalovirus(HCMV)afterbirth incubates-activate. Methods The serum levels of HCMV-DNA and HBV-DNA were determined by real-time fluorescent ration PCR, and the HCMV-IgG, IgM level were determined by ELISA in 162 serums from hepatitis patients HBsAg reported the masculine. Results The masculine rate of HCMV-DNA and HCMV-IgM, and the mean vahe of HCMV-DNA ration were much consilient: controls＜hepatitis B sufferer＜hepatocirrhosis＜liver cancer. The masculine rate of HCMV-DNA and HCMV-IgM in hepatitis B HBV-DNA positive patients was higher in hepatitis B HBV-DNA negative patients(P＜0.01). Conclusion Under the multiple infection appearance, it has the certain association with connection which HCMV afterbirth incubates-activate the infection again to hepatitis B get disease turn into.

Objective To establish a serological diagnostic method about NSCLC by investigating the expres-sion of 3 tumor markers of the sulfate glycosaminoglyean fragments(SGF), TSGF and CEA in NSCLC patients' serum. Methods To assay the level of SGF,TSGF and CEA in 50 NSCLC patients with latex particleagglutiation test,colori-metry of biochemistry and CLIA, respectively. Compared the clinical value of different examination combination way in NSCLC diagnosis value. Results There was high difference value between the Ⅰ - Ⅱ stage and the Ⅲ time,the Ⅲ time and the Ⅳ time about the level of SGF(P <0.05) in NSCLC patients. The specificity between SGF,TSGF and CEA each single item and the SGF and TSGF combination examination were not significant(P > 0.05). The semitivity between the SGF and TSGF combination and the SGF,TSGF and CEA combination was not significant(P >0.05), its specificity had significant difference(P <0.05). Conclusions There are higher expression about the 3 tumor mark-ers in NSCLC patients' serum. In the different combination examination, SGF and TSGF combined has great clinical value than other combinations to diagnosis NSCLC.

Objective MiR-375 is lowly expressed in esophageal squamous cancer cells and the downstream target gene of miR-375 remains unclear .This paper discusses the role of miR-375 in regulating the expression of short stature homobox 2 ( SHOX2) in human esophageal squamous cancer cells . Methods The bioinformatics software TargetScan , miRanda, PicTar, miRTarget2, and PITA were used to predict the assumptive targets of miR-375 in SHOX2.Then, two recombinant luciferase gene report plasmids containing wild pSHOX2 3′UTR ( pSHOX2-375-WT ) and mutant pSHOX2 3′UTR ( pSHOX2-375-mut ) were constructed , sequenced , and identified.Human esophageal squamous cancer cells were co-transfected with miR-375 mimics and pSHOX2-375-WT or pSHOX2-375-mut, respectively , and divided into 7 groups: pmiR, pSHOX2-375-WT, pSHOX2-375-WT +miR-375, pSHOX2-375-WT +miR-NC, pSHOX2-375-mut, pSHOX2-375-mut+miR-375, and pSHOX2-375-mut+miR-NC, each subjected to the measurement of luciferase activity .The expressions of SHOX 2 mRNA and protein were de-termined after transfection of the esophageal squamous cancer cells with miR-375 mimics, and so were the expressions of miR-375 and SHOX2 in the esophageal squamous carcinoma tissue samples obtained postoperatively . Results Prediction with the five software showed only one conserved function site of miR-375 in SHOX2 3′UTR at 1156-1170 bp.Luciferase activity was significantly lower in the pSHOX2-375-WT+miR-375 group (0.261 ±0.036) than in the pmiR (1.818 ±0.061), pSHOX2-375-WT (1.820 ±0.086), pSHOX2-375-WT+miR-NC (1.851 ±0.094), pSHOX2-375-mut (1.861 ±0.059), pSHOX2-375-mut +miR-375 (1.896 ± 0.048), and pSHOX2-375-mut+miR-NC group (1.760 ±0.062) ( P<0.01).SHOX2 mRNA and protein expressions were sup-pressed by the overexpression of miR-375 in the EC9706 cells.The expression of miR-375 was decreased, while that of SHOX2 in-creased in the esophageal squamous carcinoma tissue as compared with the normal esophageal tissue . Conclusion MiR-375 regu-lates the expression of the SHOX 2 gen in esophageal squamous cancer cells .

recurrence of half a year.Results The rate of symptom improvement in D group was significantly better than the other three groups(P <0.05).Over time,there was no statistical difference between four groups.The recurrence rate of D group (0.00%)was obviously lower than A group (11.76%),the B group (2.00%)and the C group (3.85%)(P <0.05).Conclusion Microscope with the traditional Chinese medicine is a good choice in preventing postoperative the rate of symptom and recurrence.

Objective To explore the correlation between hepatitis B virus large surface protein(HBV-LP)and hepatitis B virus deoxyribonucleic acid(HBV-DNA),analyze the clinical significance of detecting the two index dynamically for diagnosis and treatment of hepatitis B.Methods Enzyme linked immunosorbent assay(ELISA)and fluorescent quantitation polymerase chain reaction(FQ-PCR)were used to detect the levels of HBV-LP and HBV-DNA in serum specimen of 230 hepatitis B patients.Results There was a positive correlation between the content of HBV-LP and copy numbers of HBV-DNA in serum of hepatitis B positive cases(r=0.84,P＜0.01).The positive rate was 84.78％ in HBV-LP and 84.35％ in HBV-DNA of 230 HBV positive cases.As a result there was no significant difference(P＞0.05)in this study.Therefore,the positive rate was 63.63 ％(35/55)in HBV-LP and 58.18％(32/55)in HBV-DNA of 55 HBV negative cases.There was also no significant difference(P＞0.05)in this study.However,the positive rate of HBV-LP(82.47％)was higher than HBE(52.06％)in the 194 HBV-DNA positive cases.There was significantly different(P＜0.01).Conclusion HBV-LP and HBV-DNA are the significant index of the degree of hepatitis B virus replication in hepatitis B positive cases,especially in determining the virus replication and prognosis of treatment in HBe Ag negative,providing the reliable laboratory data for the clinical diagnosis and treatment.

Objective To study the relationship of serum leptin and inflammation with nutrition status in continuous ambulatory peritoneal dialysis(CAPD) patients.Methods 30 CAPD patients and 30 healthy controls were enrolled in the study.Leptin was measured by ELISA,inflammatory factors C reactive protein(CRP),IL-6,TNF-?.These patients were classified to three nutritional categories based on SGA normalnutrition,mild-to-moderate malnutrition,and severe malnutrition.Results CAPD group had significantly higher serum leptin,peritoneal dialysis fluid leptin,insulin,CRP,IL-6 and TNF-?,lower serum Alb than the control group.IL-6 was significantly positive correlation with TNF-?.Serum leptin was no correlation with Alb,CRP,IL-6 and TNF-?.Conclusion Serum leptin and inflammation are closely related to the occurrence of malnutrition in CAPD.

Objective To explore the relationship between homocystine(HCY) and diabetic nephropathy and uremia in the patients with type 2 diabetes mellitus.Methods 20 cases with type 2-DM,20 cases with incipient diabetic nephropathy and 17 cases with uremia were collected.The plasma levels of HCY were measured by FPIA,the fasting blood sugar were measured by standard automated clinical chemistry laboratory methods.The plasma levels of HCY were compared with each other with t test.Results The plasma levels of HCY between the type 2-DM and diabetic nephropathy were significantly different,(26.16?12.31)?mol/L vs (8.79?3.51)?mol/L,P

Objective To discuss the normal reference value of 7 parameters and its clinical significance of granulofilocytes.Methods 7 parameters of granulofilocyte using Coulter LH 755 automatic blood cytoanalyze were analyzed in 150 cases of healty people and the changes of HLR and IRF were examined in 100 cases of anaemic patients.Results There were significant difference between two groups in granulofilocyte absolute value(RET#) and high light scatter reticulocyte absolute value(HLR#)(P0.05).The mean of them in different of anaemia was 0.80%,4.01%,1.9%,0.33% and 0.30% respectively(P

Objective To explore the clinical application effect of squamous cell carcinoma antigen (SCC) ,cancer embryo antigen (CEA) and carbohydrate antigen 125(CA125) chemiluminescence combined detection in early diagnosis of lung cancer .Methods The SCC ,CEA and CA125 levels in the patients with early stage lung cancer ,benign lung diseases and healthy subjects undergoing physical examination were detected by using the automatic chemiluminescence immune analyzer .Results The concentrations of three tumor markers in the lung cancer group were significantly higher than those in the benign disease group and control group (P<0 .05);t he concentrations of CEA and CA125 in the adenocarcinoma patients with were significantly higher than those in the patients with squamous cell carcinoma and small cell carcinoma (P<0 .05) .The concentration of SCC in the patients with squamous cell carcinoma was significantly higher than that in the patients with adenocarcinoma and small cell lung cancer (P<0 .05) .The sen-sitivity ,specificity ,positive predictive value and negative predictive value of combined detection of three tumor markers were signifi-cantly better than that of single tumor marker ,and the difference was statistically significant (P<0 .05) .Conclusion The combined detection of SCC ,CEA and CA125 can be used in the early diagnosis of lung cancer and has the promotion value .