The priming of puberty is date from the increase of the secretion of hypothalamic gonadotropin-releasing hormone (GnRH). Recently,Kisspeptins/ GPR54 signaling were found critical to promote the secretion of GnRH and the onset of puberty. KISSI expresses in special area of hypothalamus and is regulated by sex steroids in the estrous cycle.Kisspeptins transitive steroid feedback signals to GnRH cells, especially the positive feedback signal which causes preovulatory GnRH / LH secretion. Kisspeptins bind KISS1R in the hypothalamic GnRH neurons and evoke the release of GnRH and the onset of puberty.

The essential nutrients include carbohydrate,protein,lipids,minerals,vitamins and water.Recently,dietary fiber has been listed as an essential component for nutrition and health.Dietary fiber plays an important role on the health of children,such as reducing constipation,preventing overweight and obesity,etc.This article reviews recent progress in research of the recommended allowance for dietary fiber in childhood.

Objective To explore the clinical effect of octreotide combined with propranlolum in the treatment of portal hypertension combined upper alimentary canal haemorrhage.Methods 63 cases with portal hypertension upper gastrointestinal hemorrhage were randomly divided into two groups,both of the two groups were given same basic medical care,while then one group was treated with octreotide combined with propranlolum,and the other group was given pituitrin.Results There was statistical difference in defecate occult blood disappear time,total effective rate between treatment group and control group (P ＜ 0.05).Conclusion Octreotide combined with propranlolum was more effective to the patients with portal hypertension upper gastrointestinal hemorrhage than pituitrin.

Objective To explore the prevalence of prehypertension and the risk factors of prehypertension in young and middle aged population of Pinghu area in Shenzhen .so as to provide the scientific basis for prevention and control of hypertension .Methods 1 498 residents who were young and middle aged in Pinghu Area in Shenzhen were recruited .The prevalence was calculated and the risk factors were analyzed with logistic regression .Results The incidence of prehypertension was 42 .90% in the population . The incidence of prehypertension in male(51 .82% ) was significantly higher than that in female(28 .27% ) .The mean levels of fast-ing plasma glucose ,total cholesterol ,triglycerides ,LDL-C ,body mass index were higher in prehypertension group than that in nor-mal blood pressure group ,HDL-C was lower than that in the normal blood pressure group .Logistic regression analysis showed that that abdominal obesity ,overweight ,triglycerides and HDL-C were the risk factors of prehypertension in men ,while abdominal obe-sity ,overweight and triglycerides were the risk factors of prehypertension in women .Conclusion Prevalence of prehypertension is higher in young and middle aged individuals of Pinghu area in Shenzhen .The abdominal obesity ,overweight and triglycerides are the most important risk factors of prehypertension .

Objective To evaluate the efficacy of mifepristone combined with cardiopulmonary suppository combined with psychological intervention in the termination of scar uterine pregnancy.Methods60 cases of maternal women with scarring uterine pregnancy during our period from March 2015 to December 2016 were selected as the experimental group in the course of this study.Maternal 60 with no scarring uterine pregnancy was selected as the control in this study group.Related medical staff need to strengthen communication with patients, understand the negative thoughts of the patient's heart, use their expertise to solve doubts for patients, eliminate anxiety and anxiety, improve patient treatment compliance, the establishment of patient treatment confidence;experimental group and control group of patients Were treated with mifepristone, oral use, the patient in the third day of drug treatment should be tested card pregnant suppository to take, the drug selected fasting medication, placed in the vagina on the dome.To compare the therapeutic effect of the experimental group and the control group and the adverse reaction.ResultsAfter treatment, 60 cases in the experimental group, the total effective number of cases in the treatment of patients with a total of 55 cases, the number of invalid cases in the treatment of patients, the effective rate was 91.7%.The control group of 60 patients, the total effective number of cases for the treatment of 48 cases, the number of invalid cases in the treatment of patients, the effective rate was 80.0%.The results showed that there was significant difference between the experimental group and the control group (P<0.05).After treatment, there was no significant difference in the time of vaginal discharge, the amount of vaginal bleeding and the time of uterine contraction.In the control group, there were 8 cases of adverse reactions, the probability was 13.3%, in the experimental group, the number of cases of adverse reactions occurred in the number of cases, the probability was 6.7%.There was significant difference between the experimental group and the control group in the incidence of adverse reactions(P<0.05).ConclusionMifepristone combined with carboprost suppository in the treatment of psychological intervention combined with the termination of good uterine scar effect, low incidence rate of adverse reaction, high treatment efficiency, high safety, with further clinical promotion and application significance.

Spinal cord injury(SCI) is a serious nervous system trauma,which may consequently lead to various degrees of paralysis and toilet obstacles,and its disability and the high cost heavily burdened the families and community.Therefore,the research on spinal cord tissue regeneration and the repair after injury has important practical significance.A large number of experimental studies have shown that neurotrophic factor plays an important role in the nerve tissue repair process.This article reviews new research progress about the effects of neurotrophic factor in this field.

OBJECTIVE:To explore the effect of different plasmapheresis supplement timing on therapeutic efficacy of toxic liver injury. METHODS:96 patients with toxic liver injury and divided into group A,B ,C and D with 32 cases in each group ac-cording to different plasmapheresis supplement timing. All patients received plasmapheresis supplement based on routine treatment. In group A,synchronized fluid replacement was 100% fresh plasma;in group B,synchronized fluid replacement was 40% normal saline firstly,and then 60%fresh plasma;in group C,substitute liquid was given till the in vitro blood reached 12%of circulation amount,supplement order as 40% normal saline for the first supplement,and then add 60% fresh plasma. The clinical symptoms and signs,liver function,prothrombin activity and blood biochemical indicators were observed in each group before and after treat-ment. RESULTS:Plasmapheresis supplement had good therapeutic efficacy on toxic liver injury;but the time of clinical symptoms and signs disappearance in group C was significantly shorter than that in group A and B,and the group B was significantly shorter the group A,with statistical significance (P<0.05). The plasma bilirubin,prothrombin activity,albumin levels of 3 groups after treatment were significantly better than before,with statistical significance(P<0.05);the group C was better than the group A and B,and the group B was better than the group A,with statistical significance(P<0.05). Compared with before treatment,the lev-els of AST and ALT in 3 groups decreased significantly after treatment,with statistical significance(P<0.05);the group B and C were better than the group A,with statistical significance(P<0.05);the group B and C was similar to each other,without statisti-cal significance(P>0.05). CONCLUSIONS:Different plasmapheresis supplement timing have different effect on toxic liver injury. The supplement method that giving 40% normal saline for the first supplement,and then add 60% fresh plasma when in vitro blood reach 12%of circulation amount has more significant effect.

BACKGROUND:We tried to combine 8-bromo-7-methoxychrysin and sorafenib in order to offset the tolerance of hepatocelular cancer stem cels to sorafenib, thereby comprehensively improving the therapeutic efficacy on hepatocelular carcinoma. OBJECTIVE:To observe the effects of 8-bromo-7-methoxychrysin, sorafenib and their combination on apoptosis of liver cancer stem-like cels SMMC-7721, and to analyze their mechanisms. METHODS:SMMC-7721 cels were treated with 8-bromo-7-methoxychrysin, sorafenib alone and their combination for 24 hours. Then, flow cytometry was used to detect cel apoptosis and western blot assay was used to determine nuclear factor-κB protein expression. RESULTS AND CONCLUSION:Compared with 8-bromo-7-methoxychrysin group and sorafenib group, the apoptotic rates of SMMC-7721 cels were significantly enhanced after treatment with the combination of 8-bromo-7-methoxychrysin group and sorafenib (2.5, 5, 10, 50 μmol/L), and meanwhile, the protein expression of nuclear factor-κB was down-regulated significantly. These findings indicate that the combined therapy of 8-bromo-7-methoxychrysin group and sorafenib can enhance the apoptosis of SMMC-7721 cels, which may be associated with down-regulation of nuclear factor-κB protein.

Objective To compare the chnical curative effect between pendant laparoscopic and traditional laparoscopic apphed in uterine fibroids divest,and analyze the risk factors of recurrence after resection of uterine fibrnids.Methods According to the different methods,130 patients with uterine fibroids were divided into suspension group (pendant laparoscopic uterine fibroids divest) and control group (traditional laparoscopic uterine fibroids divest),and 65 cases in each group.The intraoperative blood loss,operative time,exhaust time,heating duration,laparoscopic drainage were recorded,and the complications and the factors of recurrence were evaluated.Results The operative time,intraoperative blood loss,laparoscopic drainage in suspension group was less than that in control group [(53.5 ±20.9) min vs.(100.8 ± 26.7) min,(92.7 ± 18.9) ml vs.(168.4 ± 19.6) ml,(94.3 ± 13.8) ml vs.(186.5 ± 24.7) ml],the complications rate in suspension group was lower than that in control group [3.08％ (2/65) vs.33.85％ (22/65)],and there was significant difference (P ＜ 0.05).Followed up for 1-3 years,the recurrence rate was 29.23％ (38/130),3 cases were recurred within 3-6 months in control group,no recent recurrence in suspension group.Among them,the menarche age ≤13 years old,multiple myoma,operation age ＜ 35 years old were correlated with recurrence (P＜ 0.01 or ＜ 0.05).Conclusions Pendant laparoscopic uterine fibroids divest is fast and safe in operation and the prognosis effect significantly,faint pain degrees,myoma resection completely,short hospitalization period,less postoperative recurrence and other advantages,is worthy of reference for clinical promotion.The menarehe age ≤ 13 years old,multiple myoma,operation age ＜ 35 years old are correlated with recurrence.

This experiment confirmed that the cause of rupture of the anastomosis in gastroenterostomy did not depend upon the layers of suture The rupture of anastomosis did not occur between the needle suture points if the submucosa was properly sutured, it may occur when the needle suture hole was gradually enlarged and connected each other So, it is reasonable that the distance of suture points should be kept about 0 5cm If the distance is too short or the suture layers are too many, it is not good for the healing of the anastomosis