1.Reconstruction of large vermilion defects with buccinator myomucosal flap.
Jingjian HAN ; Yanyong ZHAO ; Dongshuo JI ; Mei YANG ; Ge LIU ; Haiyue JIANG
Chinese Journal of Plastic Surgery 2014;30(4):248-251
OBJECTIVETo investigate a novel method for the reconstruction of large vermilion defects.
METHODSBased on the size and shape of the defects, a buccinator myomucosal flap pedicled with the junction of buccinator and orbicularis oris in the oral commisure was designed and rotated to reconstruct the large vermilion defects. The upper bound of the flap is at least 1 cm away from the stensen's duct. The width is about 2.5-3.0 cm, and the length is as far as to arrive the raphe pterygomancibularis. The donate site is directly closed primarily. There is no need for secondary pedicle division.
RESULTSFrom July 2003 to April 2013, 14 cases with large vermilion defects was reconstructed with this method. No flap necrosis occurred with primary healing. 5 cases were followed up with an average follow up period of 1 year (0.5-3 years). The apprearance and function of the reconstructed vermilion were satisfactory without any apparent donor site defect. The patients were satisfied with both the functional and cosmetic results.
CONCLUSIONThe buccinator myomucosal flap is a simple and ideal method for reconstruction of large vermilion defects, especially for the defects closed to the commisure.
Adolescent ; Adult ; Child ; Facial Muscles ; surgery ; Female ; Follow-Up Studies ; Humans ; Lip ; surgery ; Male ; Mouth Mucosa ; surgery ; Surgical Flaps ; Young Adult
2.Protective effect of histone deacetylase inhibitor MS-275 on acute liver failure induced by D-galactosamine and lipopolysaccharide in mice
Haiyue ZHANG ; Xun LI ; Qian ZHANG ; Feifei LIU ; Luwen WANG ; Zuojiong GONG
Chinese Journal of Clinical Infectious Diseases 2016;9(4):349-354
Objective To investigate the effect of MS-275, an histone deacetylase ( HDAC ) inhibitor, on acute liver failure ( ALF ) induced by D-galactosamine and lipopolysaccharide in mice. Methods Thirty specific pathogen free (SPF) C57BL/6 male mice were randomly and equally divided into control, ALF model and MS-275 groups. ALF model was induced by D-galactosamine ( D-Gal ) and lipopolysaccharide (LPS), and the mice in MS-275 group received MS-275 (1 mg/kg) at 2 h before the induction of ALF.Serum and liver samples of mice were obtained at 24 h after ALF induction.The serum levels of ALT, AST, TBil and tumor necrosis factor-alpha ( TNF-α) , interferon γ( IFNγ) , interleukin ( IL )-1β, high mobility group box 1 ( HMGB1 ) were tested by biochemical methods or ELISA kit, respectively.The expression of HDAC1, HDAC3, acetylation of histone H3, H4, P65, acetylation and phosphorylation of P65 in liver were detected by Western blotting.The changes of histology in liver was detected by HE staining, and the translocation of P65 in liver was detected by immunohistochemistry. Comparison of variables among the groups was performed using t test.Results MS-275 inhibited the infiltration of inflammatory cells and improved the pathological changes of liver tissue.Compared with ALF group, serum ALT, AST, TBil levels were decreased in MS-275 group ( t =-22.215, -11.914 and-12.160, all P<0.05), but still higher than those in the control group (t=14.852, 11.692 and 8.333, all P<0.05); serum TNF-α, IFNγ, IL-1β, HMGB1 levels were also significantly decreased in MS-275 group (t=-7.926, -3.427, -2.475 and -5.920, all P<0.05), but TNF-αand IFNγwere still higher than those in the control group (t=5.541 and 5.514, all P<0.05).Compared with control group, the expression of class I HDAC in liver tissue was significantly decreased in MS-275 group ( t=-3.676 and-10.576, P<0.05), while the expressions of acetylation of histone H3, H4 and P65 were significantly increased (t=3.976, 5.559 and 4.588, all P<0.05).MS-275 inhibited the translocation of P65 from cytoplasm to the nucleus.Conclusion MS-275 can protect liver from acute failure in mice through enhancing the acetylation levels of non-histones.
3.Comparison of human and animal oral microbiota by Illumina sequencing of 16S rRNA tags
Dongshu GU ; Bangzhu CHEN ; Xia JIANG ; Haiyue LIU ; Bayaer NASHUN ; Hongwei ZHOU ; Weiwang GU
Chinese Journal of Comparative Medicine 2016;26(8):96-102
Objective To provide original reference data for oral ecosystem research, Tibet minipigs, beagle dogs, rhesus monkey, New Zealand white rabbits and Wistar rats were selected to study their respective characteristics of oral microbial mmunities and compared with normal data of humans.Methods Total DNA was extracted from the specimens of oral microbial communities of Tibet minipigs, beagle dogs, rhesus monkey, New Zealand white rabbits and Wistar rats, and used to amplify 16S rRNA V4 fragments with labeled universal primers.The diversity and structure of microbial communities from those animals were compared with that of humans using BIPES and QIIME analysis after Illumina sequencing of 16S rRNA V4 fragments.Results The richness of the oral microbial communities of humans and the five species of laboratory animals was significantly different (P <0.05).Different species of animals have their own unique oral flora, among which the oral flora of the monkey is the most similar to that of humans.Conclusions Among the five species of laboratory animals, the oral microbial communities of rhesus monkeys and humans have highest similarity. Specifically, the Fusobacterium and Porphyromonas levels of rhesus monkeys is most similar to those of humans.Our findings indicate that rhesus monkeys may be suitable animal model for studies of human oral microbial communities.Tibet minipigs may be suitable animal model for Proteobacteria studies, while beagle dogs may be appropriate for modeling of diseases related to Spirochaetes.
4.The application of individual earmuffs in microtia reconstruction using tissue expander
Xuehong LIU ; Haiyue JIANG ; Qinghua YANG ; Bo PAN ; Hongxing ZHUANG ; Caixia HAN ; Chunhua WANG ; Chunhua ZHANG ; Guihong LI ; Yanchun LIU ; Mei TONG ; Guihua QI
Chinese Journal of Practical Nursing 2009;25(35):8-10
Objective To explore the protective affect of individual earmuffs in microtia reconstruction using tissue expander.Methods 95 patients performed the implantation of tissue expander had been grouped into the experimental group(49 cases)and the control group(46 cases).Patients in the experimental group wore individual earmuffs,whereas patients in the control group wore traditional earmuffs.The survey including the information of patients' experience in wearing the earmuffs was carried out on the day when patients were given auricular reconstruction.Data collected from the two groups were analyzed to evaluate the aspects of permeability and safety.Results Patients in the experimental group complained less discomfort in sultry in permeability and tinnitus than patients in the control group.From the perspective of safety,there was no complaints of mosquitoes climbing into the earmuffs from the two groups.Because of the good adhesiveness of the individual earmuffs to the skin,the incidence of redness of skin in the experimen tal group was significantly lower than that in the control group.Conclusions Individual earmuff is a safe and comfortable nursing appliance which is practical for clinical application because of its good permeability,adhesiveness and less incidence of redness of skin.
5.Determination of seven components in Pifubingxuedu tablet by HPLC method
Zhihui SHI ; Huimin XIAO ; Yue HE ; Yang LIU ; Haiyue LIU ; Xu YANG ; Siwang WANG
Journal of Pharmaceutical Practice 2017;35(5):427-432
Objective To establish a HPLC method for simultaneous determination of seven constituents in Pifubingxuedu tablet.Methods The determination was performed on Kromasil-C18 column (4.6 mm × 250 mm, 5 μm).The mobile phase was acetonitrile-0.5% phosphoric acid aqueous solution with a flow rate of 0.8ml/min.The detector was PDA and detection wavelength was set at 245,325,403 nm, respectively.Results A method has been established for the determination of chlorogenic acid, hydroxy Safflower Pigment A, paeoniflorin, forsythiaside A, ferulic acid, berberine and glycyrrhizin acid in one run by HPLC.Their average contents and RSD in each Pifubingxuedu tablet were 0.299 5 mg/tablet (2.25%);0.700 0 mg/tablet(1.33%);0.429 2 mg/tablet (1.21%);0.039 1 mg/tablet (2.34%);0.014 8 mg/tablet(2.23%);0.209 0 mg/tablet (2.06%);0.272 7 mg/tablet (2.68%), respectively.Conclusion The established method is simple, convenient, accurate and reliable.It is suitable for the quality control of Pifubingxuedu tablet.
6. Biological characteristics of chondrocytes derived from auricles of different ages
Yi WU ; Kexin DONG ; Zheng YANG ; Xia LIU ; Ran XIAO ; Haiyue JIANG
Chinese Journal of Plastic Surgery 2019;35(4):331-340
Objective:
To investigate the tissue structure, chondrocyte characteristics, and the differential expression of related genes and cell surface markers of auricular cartilage of patients in different ages, in order to provide a basis for the age selection of tissue engineered cartilage repair defects.
Methods:
The auricular cartilage tissue was obtained from 22 patients with microtia in the Plastic Surgery Hospital, Chinese Academy of Medical Science, ranged from 6 to 28 years old, and divided into the child group (6-12 years old), the adolescent group (13-18 years old) and the adult group (21-28 years old). The proliferation and differentiation features of chondrocytes which from different-aged patients were detected. Furthermore, quantitative real-time PCR was used to detect the differences in the expression of genes related to cell proliferation and chondrocyte extracellular matrix. Flow cytometry, immunofluorescence and immunohistochemistry were used to detect the differences in the expression of mesenchymal stem cell markers CD90, CD44, CD73 and CD105 in chondrocytes. SPSS Statistics 21.0 software was used to process statistics.
Results:
The proliferation capability of auricular chondrocytes of children was stronger than adolescents and adults, the child group vs the adult group
7. Application of Bmp5 short ear mouse model in the study of congenital microtia
Wei LIU ; Haiyue JIANG ; Qinghua YANG ; Leren HE ; Bo PAN ; Lin LIN
Chinese Journal of Plastic Surgery 2019;35(12):1258-1262
Objective:
To study the construction of
8.Family analysis of a child with Short-rib polydactyly syndrome type III due to variant of DYNC2H1 gene.
Haiyue ZHAO ; Leilei LI ; Ruizhi LIU ; Xiao YANG
Chinese Journal of Medical Genetics 2022;39(8):881-883
OBJECTIVE:
To report on the clinical characteristics of a family of short-rib polydactyly syndrome type III and its pathogenic variants.
METHODS:
Muscle samples from the the third fetus was collected after the induction of labor, and peripheral blood samples of its parents and grandparents were also collected. Whole exome sequencing (WES) was carried out for the pedigree. Candidate variants were verified by Sanger sequencing of the family.
RESULTS:
The proband was found to harbor a c.9819+1G>A variant and a c.4625C>A variant of the DYNC2H1 gene, which were respectively inherited from its mother and father. Sanger sequencing verified that the family has fit the autosomal recessive inheritance.
CONCLUSION
The c.9819+1G>A and c.4625C>A variants of the DYNC2H1 gene probably underlay the short-rib polydactyly syndrome type 3 in the proband.
Child
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Cytoplasmic Dyneins/genetics*
;
Humans
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Mutation
;
Pedigree
;
Ribs
;
Short Rib-Polydactyly Syndrome/genetics*
9. Application of Prkra lear-3J mouse as an animal model in the study of congenital microtia
Wei LIU ; Lin LIN ; Qinghua YANG ; Bo PAN ; Leren HE ; Haiyue JIANG
Chinese Journal of Plastic Surgery 2019;35(8):804-808
Objective:
To explore the feasibility of using
10. Treatment and outcome of postoperative infection of rib cartilage framework in ear reconstruction
Hengyun SUN ; Yanyong ZHAO ; Haiyue JIANG ; Qinghua YANG ; Leren HE ; Bo PAN ; Lin LIN ; Jingjian HAN ; Ying LIU
Chinese Journal of Plastic Surgery 2017;33(3):171-174
Objective:
To investigate treatment and outcome of rib cartilage framework in ear reconstruction.
Methods:
12 cases of rib cartilage framework infection in ear reconstruction were retrospectively analysed in the latest four years. Lab examination results showed that staphylococcus aureus were found in 5 cases, coagulase negative staphylococcus in 3 cases, Klebsiella pneumonia in 2 cases, aeromonas hydrophila in 1 case and no bacteria were found in 1 case with regular culture. Debridement, systemic antibiotic therapy, saline irrigations and unobstructed drainage were utilized to treat the infection.
Results:
The average duration of dressing change was 35 days in 12 cases (12-67 days), of which six cases were cured leaving no obvious or mild change of cartilage framework. Cartilage framework was totally damaged by infection in one case, so the framework had to be removed and debridement was then carried out to control infection. Secondary repair should be taken at least 6 months later. In the rest 5 cases, frameworks were taken out in the early stage of infection. The infected portion of the cartilage was removed and the healthy part was buried subcutaneously in the chest. The expanded postauricular flap and fascia were smoothened. Secondary repair should be performed after 6 months.
Conclusions
Effective debridement, irrigations and drainage can be used to control infection of cartilage framework and maintain normal contour and structure of reconstructed auricle. With regards to severe infection, framework should be removed as early as possible and infected portion of cartilage should be cleared out, while healthy part could be used for secondary reconstruction of auricular contour after complete control of infection.